MIA_06421_1

Protein

Length

1,298 amino acids

Protein function

EGGNOG:	0QDZF		Reverse transcriptase (RNA-dependent DNA polymerase)
CGD closest match:	CAL0000185137	orf19.2669	Uncharacterized protein

Protein alignments

	%id	Aln length	E-value
UniRef50_A5DXY5	45.110%	501	1.69e-131	Uncharacterized protein n=2 Tax=Lodderomyces elongisporus (strain ATCC 11503 / CBS 2605 / JCM 1781 / NBRC 1676 / NRRL YB-4239) TaxID=379508 RepID=A5DXY5_LODEL
MCA_01665_1	37.702%	496	4.97e-94	MCA_01665_1
A0A161HJI4_9ASCO	26.721%	494	1.05e-47	Gag-pol fusion protein OS=Sugiyamaella lignohabitans GN=AWJ20_4398 PE=4 SV=1
YCH4_YEAST	33.225%	307	2.90e-50	Putative transposon Ty5-1 protein YCL074W OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=TY5A PE=5 SV=2
A0A1D8PLS1_CANAL	23.929%	560	7.26e-38	Uncharacterized protein OS=Candida albicans (strain SC5314 / ATCC MYA-2876) GN=orf19.2669 PE=4 SV=1

Mitochondrial localization by mitoprotII

Probability of mitochondrial location: 0.0207

Protein family membership

None predicted.

Domains and repeats

Domain
178 - 229
GAG-pre-integrase domain (IPR025724)
238 - 442
Ribonuclease H-like domain (IPR012337)
Integrase, catalytic core (IPR001584)
814 - 972
Reverse transcriptase, RNA-dependent DNA polymerase (IPR013103)
976 - 1,032
Reverse transcriptase, RNA-dependent DNA polymerase (IPR013103)

1 200 400 600 800 1000 1298

Detailed signature matches

IPR025724

GAG-pre-integrase domain
1. PF13976 (gag_pre-in...)
  
  178 - 229
  
  Pfam
  PF13976 (gag_pre-integrs)
IPR012337

Ribonuclease H-like domain
1. SSF53098 (Ribonucle...)
  
  243 - 420
  
  SUPERFAMILY
  SSF53098 (Ribonuclease H-like)
2. G3DSA:3.30.42...
  
  238 - 442
  
  GENE3D
  G3DSA:3.30.420.10 (G3DSA:3.30.420.10)
IPR001584

Integrase, catalytic core
1. PF00665 (rve)
  
  243 - 361
  
  Pfam
  PF00665 (rve)
2. PS50994 (INTEGRASE)
  
  241 - 411
  
  PROSITE profiles
  PS50994 (INTEGRASE)
IPR013103

Reverse transcriptase, RNA-dependent DNA polymerase
1. PF07727 (RVT_2)
  
  976 - 1,032
  
  Pfam
  PF07727 (RVT_2)
  
  814 - 972
  
  Pfam
  PF07727 (RVT_2)

no IPR

Unintegrated signatures

CYTOPLASMIC_D... (C...)

48 - 58

PHOBIUS
CYTOPLASMIC_DOMAIN (Cytoplasmic domain)
NON_CYTOPLASM... (N...)

85 - 1,298

PHOBIUS
NON_CYTOPLASMIC_DOMAIN (Non cytoplasmic domain)

1 - 24

PHOBIUS
NON_CYTOPLASMIC_DOMAIN (Non cytoplasmic domain)
SSF56672 (DNA/RNA p...)

813 - 1,012

SUPERFAMILY
SSF56672 (DNA/RNA polymerases)

1,042 - 1,217

SUPERFAMILY
SSF56672 (DNA/RNA polymerases)
TMhelix

21 - 43

TMHMM
TMhelix (TMhelix)
TRANSMEMBRANE (Tran...)

59 - 84

PHOBIUS
TRANSMEMBRANE (Transmembrane region)

25 - 47

PHOBIUS
TRANSMEMBRANE (Transmembrane region)
cd09272 (RNase_HI_R...)

1,118 - 1,249

CDD
cd09272 (RNase_HI_RT_Ty1)
mobidb-lite (disord...)

522 - 631

MobiDB Lite
mobidb-lite (disorder_prediction)

Residue annotation

active site cd09272

1,122D

active site
1,122D, 1,158E, 1,192D, 1,241D
CDD
cd09272 (RNase_HI_RT_Ty1)

1,158E

active site
1,122D, 1,158E, 1,192D, 1,241D
CDD
cd09272 (RNase_HI_RT_Ty1)

1,192D

active site
1,122D, 1,158E, 1,192D, 1,241D
CDD
cd09272 (RNase_HI_RT_Ty1)

1,241D

active site
1,122D, 1,158E, 1,192D, 1,241D
CDD
cd09272 (RNase_HI_RT_Ty1)
RNA/DNA hybrid bin...

1,122D

RNA/DNA hybrid binding site
1,122D, 1,123A, 1,124D, 1,125Y, 1,153S, 1,154N, 1,155V, 1,158E, 1,192D, 1,218V, 1,245K
CDD
cd09272 (RNase_HI_RT_Ty1)

1,123A

RNA/DNA hybrid binding site
1,122D, 1,123A, 1,124D, 1,125Y, 1,153S, 1,154N, 1,155V, 1,158E, 1,192D, 1,218V, 1,245K
CDD
cd09272 (RNase_HI_RT_Ty1)

1,124D

RNA/DNA hybrid binding site
1,122D, 1,123A, 1,124D, 1,125Y, 1,153S, 1,154N, 1,155V, 1,158E, 1,192D, 1,218V, 1,245K
CDD
cd09272 (RNase_HI_RT_Ty1)

1,125Y

RNA/DNA hybrid binding site
1,122D, 1,123A, 1,124D, 1,125Y, 1,153S, 1,154N, 1,155V, 1,158E, 1,192D, 1,218V, 1,245K
CDD
cd09272 (RNase_HI_RT_Ty1)

1,153S

RNA/DNA hybrid binding site
1,122D, 1,123A, 1,124D, 1,125Y, 1,153S, 1,154N, 1,155V, 1,158E, 1,192D, 1,218V, 1,245K
CDD
cd09272 (RNase_HI_RT_Ty1)

1,154N

RNA/DNA hybrid binding site
1,122D, 1,123A, 1,124D, 1,125Y, 1,153S, 1,154N, 1,155V, 1,158E, 1,192D, 1,218V, 1,245K
CDD
cd09272 (RNase_HI_RT_Ty1)

1,155V

RNA/DNA hybrid binding site
1,122D, 1,123A, 1,124D, 1,125Y, 1,153S, 1,154N, 1,155V, 1,158E, 1,192D, 1,218V, 1,245K
CDD
cd09272 (RNase_HI_RT_Ty1)

1,158E

RNA/DNA hybrid binding site
1,122D, 1,123A, 1,124D, 1,125Y, 1,153S, 1,154N, 1,155V, 1,158E, 1,192D, 1,218V, 1,245K
CDD
cd09272 (RNase_HI_RT_Ty1)

1,192D

RNA/DNA hybrid binding site
1,122D, 1,123A, 1,124D, 1,125Y, 1,153S, 1,154N, 1,155V, 1,158E, 1,192D, 1,218V, 1,245K
CDD
cd09272 (RNase_HI_RT_Ty1)

1,218V

RNA/DNA hybrid binding site
1,122D, 1,123A, 1,124D, 1,125Y, 1,153S, 1,154N, 1,155V, 1,158E, 1,192D, 1,218V, 1,245K
CDD
cd09272 (RNase_HI_RT_Ty1)

1,245K

RNA/DNA hybrid binding site
1,122D, 1,123A, 1,124D, 1,125Y, 1,153S, 1,154N, 1,155V, 1,158E, 1,192D, 1,218V, 1,245K
CDD
cd09272 (RNase_HI_RT_Ty1)

Protein sequence

>MIA_06421_1
MADPIVDPSSSVTHPTDTKKSALTFTYLFVFAFLLLSLILLVLLHLFPFLDSRQSVHQAFVAESAASAEAHLVVGLILLT
LSMIPEPPATSATTSPTSLIFCKAVGDIVVDVLGQDGNLLIVLYFEMFSIAGGCQFIENLDHISAVENGTTRVHAIAMGD
LYFCRFRVVLPSKPVHEVFAIEPSANLWHQRLGHASVEVLKKLSKSLSVKPTHFEVVDSHKCDACLGGKATSLPFTGTTE
KATRPLELLVSDLSGPHVATPVGHRYVLTIMDFYSRYSYVELLVKKSDASLAIRNYIARCESYFSGDSRGNRVSYFHSDN
GGEYISKDLEQFFVSKGIKHTFTVPHTPQQNGVAERLNRTLFEKAKSMLLYSHAPDYLWGEAVKCANYLRNRLPSRTTGG
VCPLQLWTGKPPSYQHMRVFGCRANVVIADAKRESKLSSNIDPGVFVGYSLDRTAYRVLVDSSIVEARDVYFDESQFPFM
KSDKELSSNASGIAFGIGCGSGSMSGPRADCNDSGTGSSVGLNSIAGSNSSSGSSFGSRRSLPDVTSVSGSGSSASSTSG
STGSSPSPSLSSSPRSSSSLVVHKPRSVRRILPSSSAPLESPTLPSLPSSPILPTSDQDPSVSSPIGPSRSDLLQVGSPL
VNLITRVILGFRNEGVISGFTRIPPSVELEIVPLDQEPPSSDLVKSLDQAGVSVDTKDDTQAILPKSRSRVIAVRLPSVT
PAKRPSSHDFVAPPSKHRCDDSAKLAASSVVPAKRRAEQESISYRRLKSLRFEYDDVALLVFTDPTTFEQAMSSEDAEFW
LEACVLEMSSLNRNRTWDLVDLPPGRKAINSKWVFKIKRKADGSIERYKARLVCIGFSQVEGIDYTETFAPVVRYETVRI
VLAIAAQFGFQVHHMDVETAFLNGDLKEDIYMRQPKGFIVKGQESKVCHLNKSLYGLKQAPLCWNEKIHGALVKLGFVRN
ESDYGVYTKGRGSAMSSLSSMFKMKDLGLVEQFLGMRVKQSPYHITVDVCRYIVDMLEEFGMQNCSSVKTPLPTRDLSDF
SESDAAADPSLYRSIIGKLIYAANCARPDLAVAVSFLCRYMQNPKSMHMEAAKHTLRYLKGSAQLGLEYRAQKVYKLVGY
SDADYAQDKQDRKSFTGYVFILSGGPITWLVESNVESEYMSLSDASKECFWINQLLSLCQIPVPLPVTMFEDNQGCIALA
QNPVFHRRTKHIDVRYHVVRHYIRSGVIHLEYLDTQVMLADMFTKNLGRVKFETLRGLLGMKAVGDSATRGGVEHAMLSQ
TSACENCWEIMVDGLCLF

GO term prediction

Biological Process

GO:0015074 DNA integration

Molecular Function

GO:0003676 nucleic acid binding

Cellular Component