MIA_06414_1

Protein

Length

1,372 amino acids

Protein function

EGGNOG:	0QDZF		Reverse transcriptase (RNA-dependent DNA polymerase)
CGD closest match:	CAL0000185137	orf19.2669	Uncharacterized protein

Protein alignments

	%id	Aln length	E-value
UniRef50_A5DXY5	48.104%	501	2.21e-143	Uncharacterized protein n=2 Tax=Lodderomyces elongisporus (strain ATCC 11503 / CBS 2605 / JCM 1781 / NBRC 1676 / NRRL YB-4239) TaxID=379508 RepID=A5DXY5_LODEL
MCA_01665_1	38.835%	515	1.54e-100	MCA_01665_1
A0A161HJI4_9ASCO	29.167%	480	6.15e-56	Gag-pol fusion protein OS=Sugiyamaella lignohabitans GN=AWJ20_4398 PE=4 SV=1
YCH4_YEAST	38.380%	284	8.99e-62	Putative transposon Ty5-1 protein YCL074W OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=TY5A PE=5 SV=2
A0A1D8PLS1_CANAL	26.101%	613	4.70e-47	Uncharacterized protein OS=Candida albicans (strain SC5314 / ATCC MYA-2876) GN=orf19.2669 PE=4 SV=1

Mitochondrial localization by mitoprotII

Probability of mitochondrial location: 0.7771
Predicted cleavage: 25

Protein family membership

None predicted.

Domains and repeats

Domain
307 - 511
Ribonuclease H-like domain (IPR012337)
Integrase, catalytic core (IPR001584)
866 - 1,108
Reverse transcriptase, RNA-dependent DNA polymerase (IPR013103)

1 200 400 600 800 1000 1200 1372

Detailed signature matches

IPR012337

Ribonuclease H-like domain
1. SSF53098 (Ribonucle...)
  
  312 - 489
  
  SUPERFAMILY
  SSF53098 (Ribonuclease H-like)
2. G3DSA:3.30.42...
  
  307 - 511
  
  GENE3D
  G3DSA:3.30.420.10 (G3DSA:3.30.420.10)
IPR001584

Integrase, catalytic core
1. PF00665 (rve)
  
  312 - 430
  
  Pfam
  PF00665 (rve)
2. PS50994 (INTEGRASE)
  
  310 - 480
  
  PROSITE profiles
  PS50994 (INTEGRASE)
IPR013103

Reverse transcriptase, RNA-dependent DNA polymerase
1. PF07727 (RVT_2)
  
  866 - 1,108
  
  Pfam
  PF07727 (RVT_2)

no IPR

Unintegrated signatures

CYTOPLASMIC_D... (C...)

1 - 26

PHOBIUS
CYTOPLASMIC_DOMAIN (Cytoplasmic domain)
NON_CYTOPLASM... (N...)

51 - 1,372

PHOBIUS
NON_CYTOPLASMIC_DOMAIN (Non cytoplasmic domain)
SSF56672 (DNA/RNA p...)

865 - 1,088

SUPERFAMILY
SSF56672 (DNA/RNA polymerases)

1,118 - 1,300

SUPERFAMILY
SSF56672 (DNA/RNA polymerases)
TMhelix

20 - 42

TMHMM
TMhelix (TMhelix)
TRANSMEMBRANE (Tran...)

27 - 50

PHOBIUS
TRANSMEMBRANE (Transmembrane region)
cd09272 (RNase_HI_R...)

1,194 - 1,332

CDD
cd09272 (RNase_HI_RT_Ty1)
mobidb-lite (disord...)

585 - 738

MobiDB Lite
mobidb-lite (disorder_prediction)

792 - 821

MobiDB Lite
mobidb-lite (disorder_prediction)

Residue annotation

active site cd09272

1,198D

active site
1,198D, 1,241E, 1,275D, 1,324D
CDD
cd09272 (RNase_HI_RT_Ty1)

1,241E

active site
1,198D, 1,241E, 1,275D, 1,324D
CDD
cd09272 (RNase_HI_RT_Ty1)

1,275D

active site
1,198D, 1,241E, 1,275D, 1,324D
CDD
cd09272 (RNase_HI_RT_Ty1)

1,324D

active site
1,198D, 1,241E, 1,275D, 1,324D
CDD
cd09272 (RNase_HI_RT_Ty1)
RNA/DNA hybrid bin...

1,198D

RNA/DNA hybrid binding site
1,198D, 1,199A, 1,200D, 1,201Y, 1,236S, 1,237S, 1,238V, 1,241E, 1,275D, 1,301V, 1,328K
CDD
cd09272 (RNase_HI_RT_Ty1)

1,199A

RNA/DNA hybrid binding site
1,198D, 1,199A, 1,200D, 1,201Y, 1,236S, 1,237S, 1,238V, 1,241E, 1,275D, 1,301V, 1,328K
CDD
cd09272 (RNase_HI_RT_Ty1)

1,200D

RNA/DNA hybrid binding site
1,198D, 1,199A, 1,200D, 1,201Y, 1,236S, 1,237S, 1,238V, 1,241E, 1,275D, 1,301V, 1,328K
CDD
cd09272 (RNase_HI_RT_Ty1)

1,201Y

RNA/DNA hybrid binding site
1,198D, 1,199A, 1,200D, 1,201Y, 1,236S, 1,237S, 1,238V, 1,241E, 1,275D, 1,301V, 1,328K
CDD
cd09272 (RNase_HI_RT_Ty1)

1,236S

RNA/DNA hybrid binding site
1,198D, 1,199A, 1,200D, 1,201Y, 1,236S, 1,237S, 1,238V, 1,241E, 1,275D, 1,301V, 1,328K
CDD
cd09272 (RNase_HI_RT_Ty1)

1,237S

RNA/DNA hybrid binding site
1,198D, 1,199A, 1,200D, 1,201Y, 1,236S, 1,237S, 1,238V, 1,241E, 1,275D, 1,301V, 1,328K
CDD
cd09272 (RNase_HI_RT_Ty1)

1,238V

RNA/DNA hybrid binding site
1,198D, 1,199A, 1,200D, 1,201Y, 1,236S, 1,237S, 1,238V, 1,241E, 1,275D, 1,301V, 1,328K
CDD
cd09272 (RNase_HI_RT_Ty1)

1,241E

RNA/DNA hybrid binding site
1,198D, 1,199A, 1,200D, 1,201Y, 1,236S, 1,237S, 1,238V, 1,241E, 1,275D, 1,301V, 1,328K
CDD
cd09272 (RNase_HI_RT_Ty1)

1,275D

RNA/DNA hybrid binding site
1,198D, 1,199A, 1,200D, 1,201Y, 1,236S, 1,237S, 1,238V, 1,241E, 1,275D, 1,301V, 1,328K
CDD
cd09272 (RNase_HI_RT_Ty1)

1,301V

RNA/DNA hybrid binding site
1,198D, 1,199A, 1,200D, 1,201Y, 1,236S, 1,237S, 1,238V, 1,241E, 1,275D, 1,301V, 1,328K
CDD
cd09272 (RNase_HI_RT_Ty1)

1,328K

RNA/DNA hybrid binding site
1,198D, 1,199A, 1,200D, 1,201Y, 1,236S, 1,237S, 1,238V, 1,241E, 1,275D, 1,301V, 1,328K
CDD
cd09272 (RNase_HI_RT_Ty1)

Protein sequence

>MIA_06414_1
MLKSATTLHAQQFHLCLNRWSVRMMAFVCLVLIFWSTQLLTLHLVILFVWPTAVDPCSSVVDSLVDSVVEPFPSDVTSLA
DPVVDSCSFDIDSMADPIVDPSSSSVHQAFVAESAASAEAHLVVGPDTFDFIHDTGATSHICNNISYFTNLRPTSTTIRG
LGSSSAKAVGDIVVDVLGQDGNPFDRVVLRDVLYVPNIPRNLFAARRAIAGGCQFIENLDHISAVENGTTRVHAIAMGDL
YFCRFRVVLPSKPVHEVFAIEPSANLGISDLAMPSLSVKPTHFEVVDSHKCDACLGGKATSLPFTGTTEKATRPLELLVS
DLSGPHVATPVGHRYVLTIMDFYSRYSYVELLVKKSDASLAIRNYIARCESYFSGDSRGNRVSYFHSDNGGEYISKDLEQ
FFVSKGIKHTFTVPHTPQQNGVAERLNRTLFEKAKSMLLYSHAPDYLWGEAVKCANYLRNRLPSRTTGGVCPLQLWTGKP
PSYQHMRVFGCRANVVIADAKRESKLSSNIDPGVFVGYSLDRTAYRIVEARDVYFDESQFPFMKSDKELSSNASGIAFGI
GCGSGSMSGPRADCNDSGTGSSVGLNSIAGSNSSSGSSFGSRRSLPDVTSVSGSGSSASSTSGSTGSSPSPSLSSSPRSS
SSLVVHKPRSVRRILPSSSAPLESPTLPSLPSSPILPSSDQDPSVSSPIGPSQSDLSQVGSPSGESDNSGDSGISQRGGD
IGFHPDVIASSPSPPSVELEIVPLDQEPPSSDLVKSLDQAGVSVDTKDDTQAILPKSRSRVVAVRLPSVTPAKRPSSHDF
VAPPSKHRCDDSAKQPASSVVPAKRRLNKSPFLIDVLNLFVLTMSSEDAEFWLEACVVEMSSLKRNRTWDLVDLPPGRKA
INSKWVFKIKRKADGSIERYKARLVCIGFSQVEGIDYTETFAPVVRYETVRIVLAIAAQFGFQVHHMDVETAFLNGDLKE
DIYMRQPKGFIVKGQESKVCHLNKSLYGLKQAPLCWNEKIHGALVKLGFVRNESDYGVYTKGRGSAMVIIALYVDDLLIA
GSSTHAIANTKSSLSSMFKMKDLGLVEQFLGMRVKQSPYHITVDVCRYIVDMLEEFGMQNCSSVKTPLPTRDLSDFSESD
AAADPSLYRSIIGKLIYAANCARPDLAVAVSFLCRYMQNPKSMHMEAAKHTLRYLKGSAQLGLEYRAQKVYKLVGYSDAD
YAQDKQDRKSFTGYVFILSGGPITWACRKQVSPASSSVESEYMSLSDASKECFWINQLLSLCQIPVPLPVTMFEDNQGCI
ALAQNPVFHRRTKHIDVRYHVVRHYIRSGVIHLEYLDTQVMLADMFTKNLGRVKFETLRGLLGMKAVGDSATREVLSMQC
CRRLQHVKIVGK

GO term prediction

Biological Process

GO:0015074 DNA integration

Molecular Function

GO:0003676 nucleic acid binding

Cellular Component