MIA_06334_1

Protein

Length

337 amino acids

Protein function

EGGNOG:	0PFIW	ZTA1	Quinone oxidoreductase
SGD closest match:	S000000250	ZTA1	Probable quinone oxidoreductase
CGD closest match:	CAL0000175885	orf19.2262	NADPH:quinone reductase

Protein alignments

	%id	Aln length	E-value
MCA_03423_1	48.148%	324	7.37e-109	MCA_03423_1
A0A060TAN2_BLAAD	43.787%	338	1.50e-94	ARAD1D20988p OS=Blastobotrys adeninivorans GN=GNLVRS02_ARAD1D20988g PE=4 SV=1
UniRef50_A0A0F8B6W8	43.731%	327	6.56e-85	Putative quinone oxidoreductase n=74 Tax=cellular organisms TaxID=131567 RepID=A0A0F8B6W8_CERFI
A0A0J9X5L3_GEOCN	44.207%	328	9.81e-88	Similar to Saccharomyces cerevisiae YBR046C ZTA1 NADPH-dependent quinone reductase OS=Geotrichum candidum GN=BN980_GECA02s05213g PE=4 SV=1
A0A167EY42_9ASCO	42.249%	329	7.72e-83	Zta1p OS=Sugiyamaella lignohabitans GN=ZTA1 PE=4 SV=1
QOR_YEAST	39.414%	307	2.06e-78	Probable quinone oxidoreductase OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=ZTA1 PE=1 SV=1
Q59Z38_CANAL	37.209%	344	5.99e-78	NADPH:quinone reductase OS=Candida albicans (strain SC5314 / ATCC MYA-2876) GN=orf19.2262 PE=4 SV=1
A0A1E3PMT2_9ASCO	39.349%	338	2.89e-77	NAD(P)-binding protein OS=Nadsonia fulvescens var. elongata DSM 6958 GN=NADFUDRAFT_82990 PE=4 SV=1
A0A1E4TC10_9ASCO	40.260%	308	1.27e-70	Uncharacterized protein OS=Tortispora caseinolytica NRRL Y-17796 GN=CANCADRAFT_137045 PE=4 SV=1
Q6CEW4_YARLI	28.065%	310	1.43e-19	YALI0B12386p OS=Yarrowia lipolytica (strain CLIB 122 / E 150) GN=YALI0_B12386g PE=4 SV=1

Mitochondrial localization by mitoprotII

Probability of mitochondrial location: 0.9640
Predicted cleavage: 23

Protein family membership

None predicted.

Domains and repeats

Domain
14 - 158
GroES-like (IPR011032)
Alcohol dehydrogenase, N-terminal (IPR013154)
165 - 290
Alcohol dehydrogenase, C-terminal (IPR013149)
Domain
24 - 335
Polyketide synthase, enoylreductase domain (IPR020843)
Domain
127 - 286
NAD(P)-binding domain (IPR016040)

1 50 100 150 200 250 300 337

Detailed signature matches

IPR011032

GroES-like
1. SSF50129 (GroES-like)
  
  14 - 158
  
  SUPERFAMILY
  SSF50129 (GroES-like)
IPR020843

Polyketide synthase, enoylreductase domain
1. SM00829 (PKS_ER_nam...)
  
  24 - 335
  
  SMART
  SM00829 (PKS_ER_names_mod)
IPR013154

Alcohol dehydrogenase, N-terminal
1. PF08240 (ADH_N)
  
  44 - 101
  
  Pfam
  PF08240 (ADH_N)
IPR016040

NAD(P)-binding domain
1. SSF51735 (NAD(P)-bi...)
  
  127 - 286
  
  SUPERFAMILY
  SSF51735 (NAD(P)-binding Rossmann-fold domains)
IPR013149

Alcohol dehydrogenase, C-terminal
1. PF00107 (ADH_zinc_N)
  
  165 - 290
  
  Pfam
  PF00107 (ADH_zinc_N)

no IPR

Unintegrated signatures

G3DSA:3.40.50...

151 - 321

GENE3D
G3DSA:3.40.50.720 (G3DSA:3.40.50.720)
G3DSA:3.90.18...

3 - 150

GENE3D
G3DSA:3.90.180.10 (G3DSA:3.90.180.10)
cd05286 (QOR2)

19 - 337

CDD
cd05286 (QOR2)

Residue annotation

NADP binding site ...

55N

NADP binding site
55N, 56F, 60Y, 136L, 140T, 143T, 161A, 164G, 165G, 166T, 185T, 186S, 190K, 205Y, 229S, 230V, 251Y, 252G, 253D, 254S, 255S, 275R, 276H, 277S, 278L, 325E, 327G
CDD
cd05286 (QOR2)

56F

NADP binding site
55N, 56F, 60Y, 136L, 140T, 143T, 161A, 164G, 165G, 166T, 185T, 186S, 190K, 205Y, 229S, 230V, 251Y, 252G, 253D, 254S, 255S, 275R, 276H, 277S, 278L, 325E, 327G
CDD
cd05286 (QOR2)

60Y

NADP binding site
55N, 56F, 60Y, 136L, 140T, 143T, 161A, 164G, 165G, 166T, 185T, 186S, 190K, 205Y, 229S, 230V, 251Y, 252G, 253D, 254S, 255S, 275R, 276H, 277S, 278L, 325E, 327G
CDD
cd05286 (QOR2)

136L

NADP binding site
55N, 56F, 60Y, 136L, 140T, 143T, 161A, 164G, 165G, 166T, 185T, 186S, 190K, 205Y, 229S, 230V, 251Y, 252G, 253D, 254S, 255S, 275R, 276H, 277S, 278L, 325E, 327G
CDD
cd05286 (QOR2)

140T

NADP binding site
55N, 56F, 60Y, 136L, 140T, 143T, 161A, 164G, 165G, 166T, 185T, 186S, 190K, 205Y, 229S, 230V, 251Y, 252G, 253D, 254S, 255S, 275R, 276H, 277S, 278L, 325E, 327G
CDD
cd05286 (QOR2)

143T

NADP binding site
55N, 56F, 60Y, 136L, 140T, 143T, 161A, 164G, 165G, 166T, 185T, 186S, 190K, 205Y, 229S, 230V, 251Y, 252G, 253D, 254S, 255S, 275R, 276H, 277S, 278L, 325E, 327G
CDD
cd05286 (QOR2)

161A

NADP binding site
55N, 56F, 60Y, 136L, 140T, 143T, 161A, 164G, 165G, 166T, 185T, 186S, 190K, 205Y, 229S, 230V, 251Y, 252G, 253D, 254S, 255S, 275R, 276H, 277S, 278L, 325E, 327G
CDD
cd05286 (QOR2)

164G

NADP binding site
55N, 56F, 60Y, 136L, 140T, 143T, 161A, 164G, 165G, 166T, 185T, 186S, 190K, 205Y, 229S, 230V, 251Y, 252G, 253D, 254S, 255S, 275R, 276H, 277S, 278L, 325E, 327G
CDD
cd05286 (QOR2)

165G

NADP binding site
55N, 56F, 60Y, 136L, 140T, 143T, 161A, 164G, 165G, 166T, 185T, 186S, 190K, 205Y, 229S, 230V, 251Y, 252G, 253D, 254S, 255S, 275R, 276H, 277S, 278L, 325E, 327G
CDD
cd05286 (QOR2)

166T

NADP binding site
55N, 56F, 60Y, 136L, 140T, 143T, 161A, 164G, 165G, 166T, 185T, 186S, 190K, 205Y, 229S, 230V, 251Y, 252G, 253D, 254S, 255S, 275R, 276H, 277S, 278L, 325E, 327G
CDD
cd05286 (QOR2)

185T

NADP binding site
55N, 56F, 60Y, 136L, 140T, 143T, 161A, 164G, 165G, 166T, 185T, 186S, 190K, 205Y, 229S, 230V, 251Y, 252G, 253D, 254S, 255S, 275R, 276H, 277S, 278L, 325E, 327G
CDD
cd05286 (QOR2)

186S

NADP binding site
55N, 56F, 60Y, 136L, 140T, 143T, 161A, 164G, 165G, 166T, 185T, 186S, 190K, 205Y, 229S, 230V, 251Y, 252G, 253D, 254S, 255S, 275R, 276H, 277S, 278L, 325E, 327G
CDD
cd05286 (QOR2)

190K

NADP binding site
55N, 56F, 60Y, 136L, 140T, 143T, 161A, 164G, 165G, 166T, 185T, 186S, 190K, 205Y, 229S, 230V, 251Y, 252G, 253D, 254S, 255S, 275R, 276H, 277S, 278L, 325E, 327G
CDD
cd05286 (QOR2)

205Y

NADP binding site
55N, 56F, 60Y, 136L, 140T, 143T, 161A, 164G, 165G, 166T, 185T, 186S, 190K, 205Y, 229S, 230V, 251Y, 252G, 253D, 254S, 255S, 275R, 276H, 277S, 278L, 325E, 327G
CDD
cd05286 (QOR2)

229S

NADP binding site
55N, 56F, 60Y, 136L, 140T, 143T, 161A, 164G, 165G, 166T, 185T, 186S, 190K, 205Y, 229S, 230V, 251Y, 252G, 253D, 254S, 255S, 275R, 276H, 277S, 278L, 325E, 327G
CDD
cd05286 (QOR2)

230V

NADP binding site
55N, 56F, 60Y, 136L, 140T, 143T, 161A, 164G, 165G, 166T, 185T, 186S, 190K, 205Y, 229S, 230V, 251Y, 252G, 253D, 254S, 255S, 275R, 276H, 277S, 278L, 325E, 327G
CDD
cd05286 (QOR2)

251Y

NADP binding site
55N, 56F, 60Y, 136L, 140T, 143T, 161A, 164G, 165G, 166T, 185T, 186S, 190K, 205Y, 229S, 230V, 251Y, 252G, 253D, 254S, 255S, 275R, 276H, 277S, 278L, 325E, 327G
CDD
cd05286 (QOR2)

252G

NADP binding site
55N, 56F, 60Y, 136L, 140T, 143T, 161A, 164G, 165G, 166T, 185T, 186S, 190K, 205Y, 229S, 230V, 251Y, 252G, 253D, 254S, 255S, 275R, 276H, 277S, 278L, 325E, 327G
CDD
cd05286 (QOR2)

253D

NADP binding site
55N, 56F, 60Y, 136L, 140T, 143T, 161A, 164G, 165G, 166T, 185T, 186S, 190K, 205Y, 229S, 230V, 251Y, 252G, 253D, 254S, 255S, 275R, 276H, 277S, 278L, 325E, 327G
CDD
cd05286 (QOR2)

254S

NADP binding site
55N, 56F, 60Y, 136L, 140T, 143T, 161A, 164G, 165G, 166T, 185T, 186S, 190K, 205Y, 229S, 230V, 251Y, 252G, 253D, 254S, 255S, 275R, 276H, 277S, 278L, 325E, 327G
CDD
cd05286 (QOR2)

255S

NADP binding site
55N, 56F, 60Y, 136L, 140T, 143T, 161A, 164G, 165G, 166T, 185T, 186S, 190K, 205Y, 229S, 230V, 251Y, 252G, 253D, 254S, 255S, 275R, 276H, 277S, 278L, 325E, 327G
CDD
cd05286 (QOR2)

275R

NADP binding site
55N, 56F, 60Y, 136L, 140T, 143T, 161A, 164G, 165G, 166T, 185T, 186S, 190K, 205Y, 229S, 230V, 251Y, 252G, 253D, 254S, 255S, 275R, 276H, 277S, 278L, 325E, 327G
CDD
cd05286 (QOR2)

276H

NADP binding site
55N, 56F, 60Y, 136L, 140T, 143T, 161A, 164G, 165G, 166T, 185T, 186S, 190K, 205Y, 229S, 230V, 251Y, 252G, 253D, 254S, 255S, 275R, 276H, 277S, 278L, 325E, 327G
CDD
cd05286 (QOR2)

277S

NADP binding site
55N, 56F, 60Y, 136L, 140T, 143T, 161A, 164G, 165G, 166T, 185T, 186S, 190K, 205Y, 229S, 230V, 251Y, 252G, 253D, 254S, 255S, 275R, 276H, 277S, 278L, 325E, 327G
CDD
cd05286 (QOR2)

278L

NADP binding site
55N, 56F, 60Y, 136L, 140T, 143T, 161A, 164G, 165G, 166T, 185T, 186S, 190K, 205Y, 229S, 230V, 251Y, 252G, 253D, 254S, 255S, 275R, 276H, 277S, 278L, 325E, 327G
CDD
cd05286 (QOR2)

325E

NADP binding site
55N, 56F, 60Y, 136L, 140T, 143T, 161A, 164G, 165G, 166T, 185T, 186S, 190K, 205Y, 229S, 230V, 251Y, 252G, 253D, 254S, 255S, 275R, 276H, 277S, 278L, 325E, 327G
CDD
cd05286 (QOR2)

327G

NADP binding site
55N, 56F, 60Y, 136L, 140T, 143T, 161A, 164G, 165G, 166T, 185T, 186S, 190K, 205Y, 229S, 230V, 251Y, 252G, 253D, 254S, 255S, 275R, 276H, 277S, 278L, 325E, 327G
CDD
cd05286 (QOR2)
dimer interface cd...

148A

dimer interface
148A, 244K, 245R, 258I, 259A, 260P, 261I, 262E, 263I, 270S, 271V, 272K, 273V, 274V, 275R, 276H, 277S, 280N, 281Y
CDD
cd05286 (QOR2)

244K

dimer interface
148A, 244K, 245R, 258I, 259A, 260P, 261I, 262E, 263I, 270S, 271V, 272K, 273V, 274V, 275R, 276H, 277S, 280N, 281Y
CDD
cd05286 (QOR2)

245R

dimer interface
148A, 244K, 245R, 258I, 259A, 260P, 261I, 262E, 263I, 270S, 271V, 272K, 273V, 274V, 275R, 276H, 277S, 280N, 281Y
CDD
cd05286 (QOR2)

258I

dimer interface
148A, 244K, 245R, 258I, 259A, 260P, 261I, 262E, 263I, 270S, 271V, 272K, 273V, 274V, 275R, 276H, 277S, 280N, 281Y
CDD
cd05286 (QOR2)

259A

dimer interface
148A, 244K, 245R, 258I, 259A, 260P, 261I, 262E, 263I, 270S, 271V, 272K, 273V, 274V, 275R, 276H, 277S, 280N, 281Y
CDD
cd05286 (QOR2)

260P

dimer interface
148A, 244K, 245R, 258I, 259A, 260P, 261I, 262E, 263I, 270S, 271V, 272K, 273V, 274V, 275R, 276H, 277S, 280N, 281Y
CDD
cd05286 (QOR2)

261I

dimer interface
148A, 244K, 245R, 258I, 259A, 260P, 261I, 262E, 263I, 270S, 271V, 272K, 273V, 274V, 275R, 276H, 277S, 280N, 281Y
CDD
cd05286 (QOR2)

262E

dimer interface
148A, 244K, 245R, 258I, 259A, 260P, 261I, 262E, 263I, 270S, 271V, 272K, 273V, 274V, 275R, 276H, 277S, 280N, 281Y
CDD
cd05286 (QOR2)

263I

dimer interface
148A, 244K, 245R, 258I, 259A, 260P, 261I, 262E, 263I, 270S, 271V, 272K, 273V, 274V, 275R, 276H, 277S, 280N, 281Y
CDD
cd05286 (QOR2)

270S

dimer interface
148A, 244K, 245R, 258I, 259A, 260P, 261I, 262E, 263I, 270S, 271V, 272K, 273V, 274V, 275R, 276H, 277S, 280N, 281Y
CDD
cd05286 (QOR2)

271V

dimer interface
148A, 244K, 245R, 258I, 259A, 260P, 261I, 262E, 263I, 270S, 271V, 272K, 273V, 274V, 275R, 276H, 277S, 280N, 281Y
CDD
cd05286 (QOR2)

272K

dimer interface
148A, 244K, 245R, 258I, 259A, 260P, 261I, 262E, 263I, 270S, 271V, 272K, 273V, 274V, 275R, 276H, 277S, 280N, 281Y
CDD
cd05286 (QOR2)

273V

dimer interface
148A, 244K, 245R, 258I, 259A, 260P, 261I, 262E, 263I, 270S, 271V, 272K, 273V, 274V, 275R, 276H, 277S, 280N, 281Y
CDD
cd05286 (QOR2)

274V

dimer interface
148A, 244K, 245R, 258I, 259A, 260P, 261I, 262E, 263I, 270S, 271V, 272K, 273V, 274V, 275R, 276H, 277S, 280N, 281Y
CDD
cd05286 (QOR2)

275R

dimer interface
148A, 244K, 245R, 258I, 259A, 260P, 261I, 262E, 263I, 270S, 271V, 272K, 273V, 274V, 275R, 276H, 277S, 280N, 281Y
CDD
cd05286 (QOR2)

276H

dimer interface
148A, 244K, 245R, 258I, 259A, 260P, 261I, 262E, 263I, 270S, 271V, 272K, 273V, 274V, 275R, 276H, 277S, 280N, 281Y
CDD
cd05286 (QOR2)

277S

dimer interface
148A, 244K, 245R, 258I, 259A, 260P, 261I, 262E, 263I, 270S, 271V, 272K, 273V, 274V, 275R, 276H, 277S, 280N, 281Y
CDD
cd05286 (QOR2)

280N

dimer interface
148A, 244K, 245R, 258I, 259A, 260P, 261I, 262E, 263I, 270S, 271V, 272K, 273V, 274V, 275R, 276H, 277S, 280N, 281Y
CDD
cd05286 (QOR2)

281Y

dimer interface
148A, 244K, 245R, 258I, 259A, 260P, 261I, 262E, 263I, 270S, 271V, 272K, 273V, 274V, 275R, 276H, 277S, 280N, 281Y
CDD
cd05286 (QOR2)

Protein sequence

>MIA_06334_1
MAQPRPLRRASTFPITQTAVRIHGPNQDPKVDIDASLPVLGEYDILVKNQYCGINFVDLYYIKGTRAIAQYPFTLGREAA
GTVIKTGAKVDKFGVGDSVVYIADGAYAQHTAVDERGRVARIADFVSAKEAAASLLQGLTAITLVREAYEIKPGDWVLVH
AAAGGTGSLVVQMAAKAGARVIGVTSVEAKVATVRENGAEAVVCYKTEDIATRVRELTGGRGVDAVFDSVGNASYKSSMD
ALAKRGTFVSYGDSSGVIAPIEIAALGEKSVKVVRHSLGNYVGTPEEWTKFTEEYWTTLKHGDLRVRVTKEYALEEVPQA
LKDLESGTTTGKLVVKV

GO term prediction

Biological Process

GO:0055114 oxidation-reduction process

Molecular Function

GO:0016491 oxidoreductase activity

Cellular Component