Protein

MIA_06295_1

Length
738 amino acids

Browser: contig11:18388-20633+

Protein function

EGGNOG:0PGWZPGUG_05777flavodoxin and radical SAM domain protein
SGD closest match:S000006128TYW1S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase
CGD closest match:CAL0000201526orf19.3470Putative tRNA 4-demethylwyosine synthase

Protein alignments

%idAln lengthE-value
A0A0J9XJY0_GEOCN64.511%6960.0Similar to Saccharomyces cerevisiae YPL207W TYW1 Protein required for the synthesis of wybutosine OS=Geotrichum candidum GN=BN980_GECA24s00516g PE=4 SV=1
MCA_06302_165.973%6730.0MCA_06302_1
A0A1E3PUG5_9ASCO57.634%7270.0Uncharacterized protein OS=Nadsonia fulvescens var. elongata DSM 6958 GN=NADFUDRAFT_39863 PE=4 SV=1
A0A161HMT6_9ASCO57.796%7440.0Tyw1p OS=Sugiyamaella lignohabitans GN=TYW1 PE=4 SV=1
A0A060TI78_BLAAD62.219%6670.0ARAD1D36762p OS=Blastobotrys adeninivorans GN=GNLVRS02_ARAD1D36762g PE=4 SV=1
Q6C3V9_YARLI59.542%6550.0YALI0E31779p OS=Yarrowia lipolytica (strain CLIB 122 / E 150) GN=YALI0_E31779g PE=4 SV=1
UniRef50_Q6C3V959.542%6550.0YALI0E31779p n=9 Tax=Ascomycota TaxID=4890 RepID=Q6C3V9_YARLI
A0A1E4TB72_9ASCO60.000%6000.0Uncharacterized protein (Fragment) OS=Tortispora caseinolytica NRRL Y-17796 GN=CANCADRAFT_18953 PE=4 SV=1
A0A1D8PPU8_CANAL55.504%6450.0Putative tRNA 4-demethylwyosine synthase OS=Candida albicans (strain SC5314 / ATCC MYA-2876) GN=orf19.3470 PE=4 SV=1
TYW1_YEAST50.218%6870.0S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=TYW1 PE=1 SV=1

Mitochondrial localization by mitoprotII

Probability of mitochondrial location: 0.1186

Protein family membership

Domains and repeats

  1. Domain
  2. Domain
1 100 200 300 400 500 600 738

Detailed signature matches

  1. Family IPR001094
    Flavodoxin-like
    1. PR00369 (FLAVODOXIN)
  2. Family IPR034556
    S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase
    1. SFLDG01071 (tRNA_wy...)
  3. Domain IPR029039
    Flavoprotein-like domain
    1. SSF52218 (Flavoprot...)
    2. G3DSA:3.40.50...
  4. Domain IPR008254
    Flavodoxin/nitric oxide synthase
    1. PS50902 (FLAVODOXIN...)
    2. PF00258 (Flavodoxin_1)
  5. Domain IPR013785
    Aldolase-type TIM barrel
    1. G3DSA:3.20.20.70
  6. Domain IPR007197
    Radical SAM
    1. PF04055 (Radical_SAM)
    2. SFLDS00029 (Radical...)
  7. Domain IPR013917
    tRNA wybutosine-synthesis
    1. PF08608 (Wyosine_form)
Unintegrated signatures no IPR
Unintegrated signatures
  1. SFLDF00284 (tRNA_wy...)
  2. SSF102114 (Radical ...)
  3. cd01335 (Radical_SAM)
  4. mobidb-lite (disord...)

Residue annotation

  1. SFLDG01071
  2. SFLDF00284
  3. SFLDS00029
  4. FeS/SAM binding si...

Protein sequence

>MIA_06295_1
MGKTLFIYEFIYFAVNILDAWHFARPVVLSAVLVFGIYKYWEFNTSNLNNQETSSNPTPNKESSKKDIINDNEKNFKEKS
LNNDTTVVKTYPLAIDFTTTENNNPKEEKVKTKRAPKVFSAKKSIAVTRKLEKLKSEIYPASLNSPVYIFYSTLTGNSEK
IAKLAYQQLSQVLNQQPTLLNIDYIADLDNYFVAPTIQDPQRPPVYLFIVPSYETESPLDYFIELLKETYNDFRVDSRSL
SSIAGFSVFGIGDRENWPEPSKYCYQGKIIDKWMSKLGAPRLFPLGMGCVKSDLNQEFDLWLQFLISTLKEEAILPEPSS
IADSDSESDDEDIESNSNATLVDVEDIGSSIEATAKSRNPDASVIKEMVGKSTPTFTALTKQGYTVVGSHSGVKICRWTK
SALRGRGSCYKYSFYGIKSHLCMETTPSLACSSKCVFCWRHGTNPVGTTWRWQVDSPEVIFEGALEGHYKKIKQMKGVPG
VVASRFAEAFQVRHCALSLVGEPIFYPYINEFVNLLHSQRISSFLVCNAQHPDELSRLKRVTQLYVSVDASNEESLKKVD
RPLHRDFWGRFLKCLDILREKKEQRTVFRLTLVSGYNMSNYDEYAKLIARGEPCFVEIKGATFCGTSKESTLTMKNIPFY
QEVKEFVENLNAALGRLGLEYGIAAEHAHSCCILIAQNKFKINGVWHTVIDYDKFFDLLEAGKEFGPMDYVAPTQSWAIY
GSEEGGFSPNDSRFVKKK

GO term prediction

Biological Process

GO:0008033 tRNA processing
GO:0055114 oxidation-reduction process

Molecular Function

GO:0003824 catalytic activity
GO:0010181 FMN binding
GO:0051536 iron-sulfur cluster binding
GO:0051539 4 iron, 4 sulfur cluster binding

Cellular Component

None predicted.