Protein

MCA_06302_1

Length
728 amino acids

Gene name: TYW1

Description: S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase

Browser: contigD:3800331-3802640+

RNA-seq: read pairs 1356, FPKM 23.0, percentile rank 45.2% (100% = highest expression)

Protein function

Annotation:TYW1S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase
KEGG:K15449TYW1 tRNA wybutosine-synthesizing protein 1 [EC:4.1.3.44]
EGGNOG:0PGWZPGUG_05777flavodoxin and radical SAM domain protein
SGD closest match:S000006128TYW1S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase
CGD closest match:CAL0000201526orf19.3470Putative tRNA 4-demethylwyosine synthase

Protein alignments

%idAln lengthE-value
MIA_06295_165.97%6730.0MIA_06295_1
A0A0J9XJY0_GEOCN63.60%7060.0Similar to Saccharomyces cerevisiae YPL207W TYW1 Protein required for the synthesis of wybutosine OS=Geotrichum candidum GN=BN980_GECA24s00516g PE=4 SV=1
A0A161HMT6_9ASCO58.87%7440.0Tyw1p OS=Sugiyamaella lignohabitans GN=TYW1 PE=4 SV=1
A0A1E3PUG5_9ASCO58.44%7050.0Uncharacterized protein OS=Nadsonia fulvescens var. elongata DSM 6958 GN=NADFUDRAFT_39863 PE=4 SV=1
A0A060TI78_BLAAD60.42%7100.0ARAD1D36762p OS=Blastobotrys adeninivorans GN=GNLVRS02_ARAD1D36762g PE=4 SV=1
Q6C3V9_YARLI56.83%7320.0YALI0E31779p OS=Yarrowia lipolytica (strain CLIB 122 / E 150) GN=YALI0_E31779g PE=4 SV=1
UniRef50_Q6C3V956.83%7320.0YALI0E31779p n=9 Tax=Ascomycota TaxID=4890 RepID=Q6C3V9_YARLI
A0A1E4TB72_9ASCO58.62%6210.0Uncharacterized protein (Fragment) OS=Tortispora caseinolytica NRRL Y-17796 GN=CANCADRAFT_18953 PE=4 SV=1
A0A1D8PPU8_CANAL53.58%6980.0Putative tRNA 4-demethylwyosine synthase OS=Candida albicans (strain SC5314 / ATCC MYA-2876) GN=orf19.3470 PE=4 SV=1
TYW1_YEAST49.37%7090.0S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=TYW1 PE=1 SV=1

Mitochondrial localization by mitoprotII

Probability of mitochondrial location: 0.0034

Protein family membership

Domains and repeats

  1. Domain
  2. Domain
1 100 200 300 400 500 600 728

Detailed signature matches

  1. Family IPR001094
    Flavodoxin-like
    1. PR00369 (FLAVODOXIN)
  2. Family IPR034556
    S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase
    1. SFLDG01071 (tRNA_wy...)
  3. Domain IPR029039
    Flavoprotein-like domain
    1. SSF52218 (Flavoprot...)
    2. G3DSA:3.40.50...
  4. Domain IPR008254
    Flavodoxin/nitric oxide synthase
    1. PS50902 (FLAVODOXIN...)
    2. PF00258 (Flavodoxin_1)
  5. Domain IPR013785
    Aldolase-type TIM barrel
    1. G3DSA:3.20.20.70
  6. Domain IPR007197
    Radical SAM
    1. PF04055 (Radical_SAM)
    2. SFLDS00029 (Radical...)
  7. Domain IPR013917
    tRNA wybutosine-synthesis
    1. PF08608 (Wyosine_form)
Unintegrated signatures no IPR
Unintegrated signatures
  1. NON_CYTOPLASM... (N...)
  2. SFLDF00284 (tRNA_wy...)
  3. SIGNAL_PEPTIDE (Sig...)
  4. SIGNAL_PEPTID... (S...)
  5. SIGNAL_PEPTID... (S...)
  6. SIGNAL_PEPTID... (S...)
  7. SSF102114 (Radical ...)
  8. SignalP-noTM
  9. cd01335 (Radical_SAM)
  10. mobidb-lite (disord...)

Residue annotation

  1. SFLDF00284
  2. SFLDG01071
  3. SFLDS00029
  4. FeS/SAM binding si...

Protein sequence

>MCA_06302_1
MGIPLFLFAFVIVIAINLIPVPEDTHNEKAKKVEKPIQISKPVKETKQNPILAVDFTTSSSSAVPSSRKLNSTKRAPKVF
TAKSSIAVTKQLQKLTNNNASNAHLLESPIHIFYSTLTGTSERIANQVFKRLSSDETPILKNRKIYMDNIDYIEDLDDYF
ATPKKKNEDDAPEIYLFIVPSYETESPLDFFIESLKENYNDFRLDSRPLGKSIAGFCVFGIGDRESWPESEGKFCYQAMQ
VDKYLSKLGGRRIFPVGTGCVKTDLENQIDTWVTRFLSTLEEGNSIAEPIDIADSDDEEDVDEDFEEGSDDLSSGESTLI
DVEDIGNSIEATKKSRKINKLSSENTVKEMVAMNSPTYKALTKQGYTVVGSHSGVKICRWTKSALRGRGSCYKFSFYGIR
SHLCMETTPSLSCSNKCVFCWRHGTNPVGTTWRWKVDPPDVIFQGALEGHYKKIKQMRGVPGVVAERFEEAFQVKHCALS
LVGEPIFYPHINEFVNLLHGQGISSFLVCNAQHPEQLKNMKRVTQLYVSIDAPNKEALRKVDRPLNRDFWERFLECLDIL
REKKTQRTVFRLTLVAGYNMDDYDEYAELIARGEPCFVEVKGATFCGTSKSNPLTMKNVPFYKEVVEFVENLNAALARRG
LDYGIAAEHAHSCCILMAQGRFKIEDRWHTVIDYKKFFELLKKEEEEGIEFGPMDYLQPTASWAIYGSKEGGFSPEDVRH
RRGKVVEE

GO term prediction

Biological Process

GO:0008033 tRNA processing
GO:0055114 oxidation-reduction process

Molecular Function

GO:0003824 catalytic activity
GO:0010181 FMN binding
GO:0051536 iron-sulfur cluster binding
GO:0051539 4 iron, 4 sulfur cluster binding

Cellular Component

None predicted.