Protein

MIA_05896_1

Length
689 amino acids

Browser: contig09:642907-644977+

Protein function

EGGNOG:0PH1KLYS4Responsible for the dehydration of cis-homoaconitate to homoisocitric acid (By similarity)
SGD closest match:S000002642LYS4Homoaconitase, mitochondrial
CGD closest match:CAL0000195924LYS4Homoaconitase, mitochondrial

Protein alignments

%idAln lengthE-value
MCA_04705_185.965%6840.0MCA_04705_1
A0A0J9XHA5_GEOCN79.536%6890.0Homoaconitase, mitochondrial OS=Geotrichum candidum GN=BN980_GECA16s02177g PE=3 SV=1
LYS4_YARLI77.616%6880.0Homoaconitase, mitochondrial OS=Yarrowia lipolytica (strain CLIB 122 / E 150) GN=LYS4 PE=3 SV=1
A0A167CU71_9ASCO76.304%6710.0Homoaconitase, mitochondrial OS=Sugiyamaella lignohabitans GN=LYS4 PE=3 SV=1
A0A060TC05_BLAAD74.926%6780.0Homoaconitase, mitochondrial OS=Blastobotrys adeninivorans GN=GNLVRS02_ARAD1B12980g PE=3 SV=1
A0A1E3PKR7_9ASCO73.837%6880.0Homoaconitase, mitochondrial OS=Nadsonia fulvescens var. elongata DSM 6958 GN=NADFUDRAFT_51169 PE=3 SV=1
LYS4_CANAL71.366%6810.0Homoaconitase, mitochondrial OS=Candida albicans (strain SC5314 / ATCC MYA-2876) GN=LYS4 PE=3 SV=1
A0A1E4TDG5_9ASCO69.839%6830.0Homoaconitase, mitochondrial OS=Tortispora caseinolytica NRRL Y-17796 GN=CANCADRAFT_144314 PE=3 SV=1
LYS4_YEAST69.297%6970.0Homoaconitase, mitochondrial OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=LYS4 PE=1 SV=1
UniRef50_P4936769.297%6970.0Homoaconitase, mitochondrial n=264 Tax=root TaxID=1 RepID=LYS4_YEAST

Mitochondrial localization by mitoprotII

Probability of mitochondrial location: 0.9537
Predicted cleavage: 28

Protein family membership

Domains and repeats

  1. Domain
  2. Domain
1 100 200 300 400 500 600 689

Detailed signature matches

  1. Family IPR004418
    Homoaconitase, mitochondrial
    1. TIGR00139
  2. Domain IPR015931
    Aconitase/3-isopropylmalate dehydratase large subunit, alpha/beta/alpha, subdomain 1/3
    1. G3DSA:3.30.49...
  3. Domain IPR001030
    Aconitase/3-isopropylmalate dehydratase large subunit, alpha/beta/alpha domain
    1. PR00415 (ACONITASE)
    2. SSF53732 (Aconitase...)
    3. PF00330 (Aconitase)
  4. Domain IPR015932
    Aconitase/3-isopropylmalate dehydratase large subunit, alpha/beta/alpha, subdomain 2
    1. G3DSA:3.40.10...
  5. Domain IPR015928
    Aconitase/3-isopropylmalate dehydratase, swivel
    1. G3DSA:3.20.19.10
    2. SSF52016 (LeuD/IlvD...)
  6. Domain IPR000573
    Aconitase A/isopropylmalate dehydratase small subunit, swivel domain
    1. PF00694 (Aconitase_C)
  7. SITE IPR018136
    Aconitase family, 4Fe-4S cluster binding site
    1. PS00450 (ACONITASE_1)
    2. PS01244 (ACONITASE_2)
Unintegrated signatures no IPR
Unintegrated signatures
  1. cd01582 (Homoaconitase)
  2. cd01674 (Homoaconit...)

Residue annotation

  1. substrate binding ...
  2. ligand binding sit...
  3. substrate binding ...

Protein sequence

>MIA_05896_1
MISSIRLQSFARSMITRRTLATAAGIPQNLTEKIVQKYAVGLAPGKKVQSGDYVSVQPSHCMTHDNSWPVATKFMGLGAT
RVKDTNQVVLTLDHDVQNKSAKNLEKYRNIENFAKKQGLDFYPAGRGIGHQIMVEEGYAFPYNLTVASDSHSNTYGGVGC
LGTPIVRTDAASVWATGQTWWQVPPVAKVELKGKLPAGASGKDVIVALCGIFNNDEVLNHAIEFVGDGLEHLSVDERLTI
ANMTTEWGALSGLFPVDNVLINWYKNRLLVLPQPHPRVNAETVEAFQKAQIHADADAKYAKKLTLDLSTVSPIVSGPNSV
KVSTSLAELSAKNISIQKAYLVSCTNSRLSDIEAAAKVIKGRKVADGVEFYVGAASSVVQKDAEKNGAWQTLLEAGAIPL
PAGCGPCIGLGAGLLKDGEVGISATNRNFKGRMGSRDALAYLASPEVVAASAVLGKIGFPEEIEGKTPVNTGGIKHSIEY
PAADASAAPAEAEGSAASSGSILEGFPEVIEGELVLCDADNINTDAIYPGKYTYEDDVPKEKMAQVCMENYDPDFGSKTH
AGDVIISGFNFGTGSSREQAATCILARGIQLVVAGSFGNIFSRNSINNALLTLEIPALINMLREKYGENKEVTQRTGWFI
KWDVKSSTVIVTDGKNGPVVLKQKVGELGKNLQEIIVKGGLEGWVKAQL

GO term prediction

Biological Process

GO:0008152 metabolic process
GO:0009085 lysine biosynthetic process

Molecular Function

GO:0004409 homoaconitate hydratase activity
GO:0051539 4 iron, 4 sulfur cluster binding

Cellular Component

None predicted.