MIA_05846_1

Protein

Length

2,086 amino acids

Protein function

EGGNOG:	0PG3R	SEC7	guanyl-nucleotide exchange factor
SGD closest match:	S000002577	SEC7	Protein transport protein SEC7
CGD closest match:	CAL0000201823	SEC7	Arf family guanine nucleotide exchange factor

Protein alignments

	%id	Aln length
MCA_04003_1	69.219%	1972	MCA_04003_1
A0A0J9X8V4_GEOCN	61.686%	1874	Similar to Saccharomyces cerevisiae YDR170C SEC7 Guanine nucleotide exchange factor (GEF) for ADP ribosylation factors involved in proliferation of the Golgi, intra-Golgi and ER-to-Golgi transport OS=Geotrichum candidum GN=BN980_GECA04s06247g PE=4 SV=1
UniRef50_A0A0J9X7S5	57.250%	1738	Similar to Saccharomyces cerevisiae YDR170C SEC7 Guanine nucleotide exchange factor (GEF) for ADP ribosylation factors involved in proliferation of the Golgi, intra-Golgi and ER-to-Golgi transport n=2 Tax=Geotrichum candidum TaxID=1173061 RepID=A0A0J9X7S5_GEOCN
A0A060T737_BLAAD	51.451%	1895	ARAD1C25608p OS=Blastobotrys adeninivorans GN=GNLVRS02_ARAD1C25608g PE=4 SV=1
A0A1E3PSN5_9ASCO	50.402%	1865	Uncharacterized protein OS=Nadsonia fulvescens var. elongata DSM 6958 GN=NADFUDRAFT_54895 PE=4 SV=1
Q6C820_YARLI	45.381%	1862	YALI0D23463p OS=Yarrowia lipolytica (strain CLIB 122 / E 150) GN=YALI0_D23463g PE=4 SV=1
A0A167F5J2_9ASCO	58.518%	1174	Arf family guanine nucleotide exchange factor SEC7 OS=Sugiyamaella lignohabitans GN=SEC7 PE=4 SV=1
SEC7_YEAST	40.956%	1946	Protein transport protein SEC7 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=SEC7 PE=1 SV=2
Q5ANF9_CANAL	46.436%	1529	Arf family guanine nucleotide exchange factor OS=Candida albicans (strain SC5314 / ATCC MYA-2876) GN=SEC7 PE=4 SV=1
A0A1E4TCD9_9ASCO	38.921%	1891	Uncharacterized protein OS=Tortispora caseinolytica NRRL Y-17796 GN=CANCADRAFT_139370 PE=4 SV=1

Mitochondrial localization by mitoprotII

Probability of mitochondrial location: 0.0287

Protein family membership

None predicted.

Domains and repeats

Domain
281 - 428
Mon2, dimerisation and cyclophilin-binding domain (IPR032629)
505 - 671
Guanine nucleotide exchange factor, N-terminal (IPR032691)
817 - 1,011
Sec7 domain (IPR000904)
1,083 - 1,920
Armadillo-type fold (IPR016024)
Armadillo-like helical (IPR011989)
1,990 - 2,078
Armadillo-type fold (IPR016024)
Domain
901 - 1,019
Sec7 domain, alpha orthogonal bundle (IPR023394)
1,390 - 1,472
Sec7, C-terminal (IPR015403)

1 200 400 600 800 1000 1200 1400 1600 1800 2086

Detailed signature matches

IPR032629

Mon2, dimerisation and cyclophilin-binding domain
1. PF16213 (DCB)
  
  281 - 428
  
  Pfam
  PF16213 (DCB)
IPR032691

Guanine nucleotide exchange factor, N-terminal
1. PF12783 (Sec7_N)
  
  505 - 671
  
  Pfam
  PF12783 (Sec7_N)
IPR000904

Sec7 domain
1. SM00222 (sec7_5)
  
  823 - 1,009
  
  SMART
  SM00222 (sec7_5)
2. SSF48425 (Sec7 domain)
  
  817 - 1,011
  
  SUPERFAMILY
  SSF48425 (Sec7 domain)
3. PS50190 (SEC7)
  
  819 - 1,007
  
  PROSITE profiles
  PS50190 (SEC7)
4. PF01369 (Sec7)
  
  827 - 1,009
  
  Pfam
  PF01369 (Sec7)
IPR023394

Sec7 domain, alpha orthogonal bundle
1. G3DSA:1.10.10...
  
  901 - 1,019
  
  GENE3D
  G3DSA:1.10.1000.11 (G3DSA:1.10.1000.11)
IPR016024

Armadillo-type fold
1. SSF48371 (ARM repeat)
  
  1,354 - 1,703
  
  SUPERFAMILY
  SSF48371 (ARM repeat)
  
  1,990 - 2,078
  
  SUPERFAMILY
  SSF48371 (ARM repeat)
  
  1,780 - 1,805
  
  SUPERFAMILY
  SSF48371 (ARM repeat)
IPR011989

Armadillo-like helical
1. G3DSA:1.25.10.10
  
  1,083 - 1,920
  
  GENE3D
  G3DSA:1.25.10.10 (G3DSA:1.25.10.10)
IPR015403

Sec7, C-terminal
1. PF09324 (DUF1981)
  
  1,390 - 1,472
  
  Pfam
  PF09324 (DUF1981)

no IPR

Unintegrated signatures

Coil

820 - 840

COILS
Coil (Coil)
G3DSA:1.10.22...

805 - 900

GENE3D
G3DSA:1.10.220.20 (G3DSA:1.10.220.20)
cd00171 (Sec7)

826 - 1,009

CDD
cd00171 (Sec7)
mobidb-lite (disord...)

777 - 815

MobiDB Lite
mobidb-lite (disorder_prediction)

1 - 219

MobiDB Lite
mobidb-lite (disorder_prediction)

1,223 - 1,246

MobiDB Lite
mobidb-lite (disorder_prediction)

1,818 - 1,842

MobiDB Lite
mobidb-lite (disorder_prediction)

434 - 465

MobiDB Lite
mobidb-lite (disorder_prediction)

1,748 - 1,776

MobiDB Lite
mobidb-lite (disorder_prediction)

Residue annotation

active site/putati...

917F

active site/putative ARF binding site
917F, 918R, 919L, 920P, 921G, 922E, 923A, 924Q, 953V, 954L, 955A, 956Y, 957S, 958V, 959I, 960M, 961L, 962N, 963T, 964D, 965A, 966H
CDD
cd00171 (Sec7)

918R

active site/putative ARF binding site
917F, 918R, 919L, 920P, 921G, 922E, 923A, 924Q, 953V, 954L, 955A, 956Y, 957S, 958V, 959I, 960M, 961L, 962N, 963T, 964D, 965A, 966H
CDD
cd00171 (Sec7)

919L

active site/putative ARF binding site
917F, 918R, 919L, 920P, 921G, 922E, 923A, 924Q, 953V, 954L, 955A, 956Y, 957S, 958V, 959I, 960M, 961L, 962N, 963T, 964D, 965A, 966H
CDD
cd00171 (Sec7)

920P

active site/putative ARF binding site
917F, 918R, 919L, 920P, 921G, 922E, 923A, 924Q, 953V, 954L, 955A, 956Y, 957S, 958V, 959I, 960M, 961L, 962N, 963T, 964D, 965A, 966H
CDD
cd00171 (Sec7)

921G

active site/putative ARF binding site
917F, 918R, 919L, 920P, 921G, 922E, 923A, 924Q, 953V, 954L, 955A, 956Y, 957S, 958V, 959I, 960M, 961L, 962N, 963T, 964D, 965A, 966H
CDD
cd00171 (Sec7)

922E

active site/putative ARF binding site
917F, 918R, 919L, 920P, 921G, 922E, 923A, 924Q, 953V, 954L, 955A, 956Y, 957S, 958V, 959I, 960M, 961L, 962N, 963T, 964D, 965A, 966H
CDD
cd00171 (Sec7)

923A

active site/putative ARF binding site
917F, 918R, 919L, 920P, 921G, 922E, 923A, 924Q, 953V, 954L, 955A, 956Y, 957S, 958V, 959I, 960M, 961L, 962N, 963T, 964D, 965A, 966H
CDD
cd00171 (Sec7)

924Q

active site/putative ARF binding site
917F, 918R, 919L, 920P, 921G, 922E, 923A, 924Q, 953V, 954L, 955A, 956Y, 957S, 958V, 959I, 960M, 961L, 962N, 963T, 964D, 965A, 966H
CDD
cd00171 (Sec7)

953V

active site/putative ARF binding site
917F, 918R, 919L, 920P, 921G, 922E, 923A, 924Q, 953V, 954L, 955A, 956Y, 957S, 958V, 959I, 960M, 961L, 962N, 963T, 964D, 965A, 966H
CDD
cd00171 (Sec7)

954L

active site/putative ARF binding site
917F, 918R, 919L, 920P, 921G, 922E, 923A, 924Q, 953V, 954L, 955A, 956Y, 957S, 958V, 959I, 960M, 961L, 962N, 963T, 964D, 965A, 966H
CDD
cd00171 (Sec7)

955A

active site/putative ARF binding site
917F, 918R, 919L, 920P, 921G, 922E, 923A, 924Q, 953V, 954L, 955A, 956Y, 957S, 958V, 959I, 960M, 961L, 962N, 963T, 964D, 965A, 966H
CDD
cd00171 (Sec7)

956Y

active site/putative ARF binding site
917F, 918R, 919L, 920P, 921G, 922E, 923A, 924Q, 953V, 954L, 955A, 956Y, 957S, 958V, 959I, 960M, 961L, 962N, 963T, 964D, 965A, 966H
CDD
cd00171 (Sec7)

957S

active site/putative ARF binding site
917F, 918R, 919L, 920P, 921G, 922E, 923A, 924Q, 953V, 954L, 955A, 956Y, 957S, 958V, 959I, 960M, 961L, 962N, 963T, 964D, 965A, 966H
CDD
cd00171 (Sec7)

958V

active site/putative ARF binding site
917F, 918R, 919L, 920P, 921G, 922E, 923A, 924Q, 953V, 954L, 955A, 956Y, 957S, 958V, 959I, 960M, 961L, 962N, 963T, 964D, 965A, 966H
CDD
cd00171 (Sec7)

959I

active site/putative ARF binding site
917F, 918R, 919L, 920P, 921G, 922E, 923A, 924Q, 953V, 954L, 955A, 956Y, 957S, 958V, 959I, 960M, 961L, 962N, 963T, 964D, 965A, 966H
CDD
cd00171 (Sec7)

960M

active site/putative ARF binding site
917F, 918R, 919L, 920P, 921G, 922E, 923A, 924Q, 953V, 954L, 955A, 956Y, 957S, 958V, 959I, 960M, 961L, 962N, 963T, 964D, 965A, 966H
CDD
cd00171 (Sec7)

961L

active site/putative ARF binding site
917F, 918R, 919L, 920P, 921G, 922E, 923A, 924Q, 953V, 954L, 955A, 956Y, 957S, 958V, 959I, 960M, 961L, 962N, 963T, 964D, 965A, 966H
CDD
cd00171 (Sec7)

962N

active site/putative ARF binding site
917F, 918R, 919L, 920P, 921G, 922E, 923A, 924Q, 953V, 954L, 955A, 956Y, 957S, 958V, 959I, 960M, 961L, 962N, 963T, 964D, 965A, 966H
CDD
cd00171 (Sec7)

963T

active site/putative ARF binding site
917F, 918R, 919L, 920P, 921G, 922E, 923A, 924Q, 953V, 954L, 955A, 956Y, 957S, 958V, 959I, 960M, 961L, 962N, 963T, 964D, 965A, 966H
CDD
cd00171 (Sec7)

964D

active site/putative ARF binding site
917F, 918R, 919L, 920P, 921G, 922E, 923A, 924Q, 953V, 954L, 955A, 956Y, 957S, 958V, 959I, 960M, 961L, 962N, 963T, 964D, 965A, 966H
CDD
cd00171 (Sec7)

965A

active site/putative ARF binding site
917F, 918R, 919L, 920P, 921G, 922E, 923A, 924Q, 953V, 954L, 955A, 956Y, 957S, 958V, 959I, 960M, 961L, 962N, 963T, 964D, 965A, 966H
CDD
cd00171 (Sec7)

966H

active site/putative ARF binding site
917F, 918R, 919L, 920P, 921G, 922E, 923A, 924Q, 953V, 954L, 955A, 956Y, 957S, 958V, 959I, 960M, 961L, 962N, 963T, 964D, 965A, 966H
CDD
cd00171 (Sec7)

Protein sequence

>MIA_05846_1
MADDSETAAPAAAPAAAKPESTSESLEHEPPIQHDEPAQEPTPATNLAENEDSNNNDDDNEPPVSPVEPSSTPSTEAPPS
PSSVQPTAGSESSTQEPIIEQETANTDSQLDSQQEQEDNSEDGLKNDKVAPPELSGATIPINVTSSSEQDSVPAPASSAP
TDALPDTPSYPAFPAPVSSTAAATPSDPSSSTATVDDDVANENATVSPTRTGRRSRRSPSLALSLANVTVSGQTIVSSSV
FIKKALSTIKAHKSTASHPALDGSVTKALSIFNDNTLPPPSVVFEPLRIVCSQSSSNDLKVVALDCIGKLFSFSYLVDMD
SPLPPTPSTQDPTSGITQPPRVPIIDRAIKTVCDCFQGEYTDPRVELQIIKALMAAVLNDDLIAHGATLLRAIRQTYTIF
VLSHTMTNQATAQATLSQMIGVIFDRVKSVIKNSPSSQKASEEPENESTPQLEKTVATAPESSSIHGKLTLRQMENMGST
AELERVKEEVTSTPTDAADENDLFAKDAFLVFRTLCRLSEKTLEGDSQDNRSHGMRSKLLSLHLIHTILKSHMSLFLSHE
VLIRSNLKGDETFLVSVKDYICSTLARNAASINPAVFEISTEIFFLVVSNLRFQFKKEIEVFFSEIYFPIAEMKTSTYHQ
KQYFLSIVQKLCNDPRALVEIYLNYDCDSSSTLNIYEQLIDFLVRLAVTPVHLTVLQFQQYHELKNKPIAHYMLSSSPAI
AITNFQLNAGLHETLPYPIEHALKMTALESLVAVLRSLLTWSQRGIAAVSASLNQADEETQKRLRSSSQAGDDTPENDSK
SILSTATGSTTVVGDDPSQFQNLKIKKNALQDAIRQFNVKPKRGIKALLDTGIIESKNPEDIARFLLTTEGLDKALIGEY
LGEGDPENIAIMHAFVDLMDFTDAKFVAALRTFLQSFRLPGEAQKIDRYMLKFAERYTLNNPSIFSNADTAYVLAYSVIM
LNTDAHSPQVKNRMTLDDFVRNNRGINDGQDMPKDFLAEIYNEIQQNEIKLQSEQHAALLSSDGQQNTSGGGFASNLGNI
LAGRDLQREAYLQASREMANKTEQLFKELVSGDGKRRTAESTFYSASHIEHVKPMFEVAWMSFLAGLSGPFQQSEDPDVV
KLCLEGFKLSIRIACLFDIDLARISFVSALTQFTNLQNLSEMKQKNVDAVKQLLQTALSDGNLLRSSWKEILTCISQLER
FQLISSGIEAGAIPDVTNARFASRKPSSDTLNSGRSRSSNAPGLFSRHSTQTAYNTQYSANGALATHYGDKPFTYSPELA
QELQSREVILDMDKIFTNSALLSGDAIMDFVRALTEVSGEEIASSGQSEHPRMFSLQKMVDISYYNMNRIRVEWSRLWVI
MGKQFNVVCCHQNTNVVFFALDSLRQLSIRFFDLEELTHFKFQKEFLEPFAYVMEHNNNASVKDMVLQCLQQMVLAKSDK
IKSGWKTMFATFSIAAKQQYGHVVTTTYDIVRRIYKERLNEVIAQDDFGNLIQCFSDLAKNQRFQKPSLHAVDLLRNTIP
RILELRDEELKRNAGGELTIDFYNRYWFPVLFALHDIIMNGEDLEVRSRALNYMFDTLTEYGSGFTPEFWDSVCKKLLFP
TFVVLKNRSEMARFNTQDDISVWLSTTMIQALRNMIALLSHYFEVLSGMLDGFLELLTTCIIQENDTVSRIGSSCLQQLI
QQNVDKLQESHWKTIVDTIENLFKLTTASELFGDYSDIDDPVDQDGSKISVNGGNSTAEVPVDSHGVEEHLSAPMSPSEA
SNSTSTKQVRRKTSWSDVNHNRQKRFRKTIVKCILQLLMIDTVDEVLQRQEINQIDETANGNGSTVPSRTQSSNGEAKNT
PPPALVIRKQVKVVGTEIYEKMPVSQLLRVLVLLRKSFIFAHRFNSNKELRTNLWRLGFMKQLPNLLKQESLSAHVYISV
LIRLYTEDSKLYGQLLAAGDATKLATPIVVENYDKQFPSKLIAAEARNYAPLDDDLEPKTIEIESTTELKAYVEGQLIPM
LSDLLAIFDRLDYNDARAIQAWTPVIVSILEGLLTFSQSDFSKVIEGMYPGILGIMNRDMSPTLRLMLQSTLKRVGKVLF
KGKSKS

GO term prediction

Biological Process

GO:0032012 regulation of ARF protein signal transduction

Molecular Function

GO:0005086 ARF guanyl-nucleotide exchange factor activity
GO:0005488 binding

Cellular Component