MIA_05807_1

Protein

Length

492 amino acids

Protein function

EGGNOG:	0PH73	MSY1	tyrosyl-tRNA synthetase
SGD closest match:	S000006018	MSY1	Tyrosine--tRNA ligase, mitochondrial
CGD closest match:	CAL0000176983	orf19.109	Tyrosine--tRNA ligase

Protein alignments

	%id	Aln length	E-value
MCA_00503_1	61.494%	522	0.0	MCA_00503_1
A0A0J9XJ15_GEOCN	59.748%	477	0.0	Tyrosine--tRNA ligase OS=Geotrichum candidum GN=BN980_GECA18s00593g PE=3 SV=1
A0A060T3W9_BLAAD	56.592%	493	0.0	Tyrosine--tRNA ligase OS=Blastobotrys adeninivorans GN=GNLVRS02_ARAD1C37092g PE=3 SV=1
A0A161HN82_9ASCO	59.629%	431	0.0	Tyrosine--tRNA ligase OS=Sugiyamaella lignohabitans GN=MSY1 PE=3 SV=1
UniRef50_A0A161HN82	59.629%	431	0.0	Tyrosine--tRNA ligase n=3 Tax=Saccharomycetales TaxID=4892 RepID=A0A161HN82_9ASCO
Q59WU2_CANAL	48.085%	470	3.11e-152	Tyrosine--tRNA ligase OS=Candida albicans (strain SC5314 / ATCC MYA-2876) GN=orf19.109 PE=3 SV=1
A0A1E3PQ19_9ASCO	45.022%	462	7.43e-146	Tyrosine--tRNA ligase OS=Nadsonia fulvescens var. elongata DSM 6958 GN=NADFUDRAFT_81843 PE=3 SV=1
A0A1E4TKN4_9ASCO	45.043%	464	1.53e-136	Tyrosine--tRNA ligase OS=Tortispora caseinolytica NRRL Y-17796 GN=CANCADRAFT_84858 PE=3 SV=1
SYYM_YEAST	44.731%	465	3.30e-133	Tyrosine--tRNA ligase, mitochondrial OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=MSY1 PE=1 SV=1
Q6C474_YARLI	42.704%	466	4.84e-127	Tyrosine--tRNA ligase OS=Yarrowia lipolytica (strain CLIB 122 / E 150) GN=YALI0_E29139g PE=3 SV=1

Mitochondrial localization by mitoprotII

Probability of mitochondrial location: 0.9355
Predicted cleavage: 27

Protein family membership

Aminoacyl-tRNA synthetase, class Ic (IPR002305)
- Tyrosine-tRNA ligase (IPR002307)

Domains and repeats

Domain
32 - 293
Rossmann-like alpha/beta/alpha sandwich fold (IPR014729)
395 - 492
RNA-binding S4 domain (IPR002942)

1 50 100 150 200 250 300 350 400 450 492

Detailed signature matches

IPR002305

Aminoacyl-tRNA synthetase, class Ic
1. PF00579 (tRNA-synt_1b)
  
  63 - 387
  
  Pfam
  PF00579 (tRNA-synt_1b)
IPR002307

Tyrosine-tRNA ligase
1. TIGR00234
  
  40 - 489
  
  TIGRFAMs
  TIGR00234 (TIGR00234)
2. cd00805 (TyrRS_core)
  
  66 - 373
  
  CDD
  cd00805 (TyrRS_core)
3. PR01040 (TRNASYNTHTYR)
  
  209 - 224
  
  PRINTS
  PR01040 (TRNASYNTHTYR)
  
  230 - 252
  
  PRINTS
  PR01040 (TRNASYNTHTYR)
  
  78 - 100
  
  PRINTS
  PR01040 (TRNASYNTHTYR)
IPR024107

Tyrosine-tRNA ligase, bacterial-type, type 1
1. MF_02006 (Tyr_tRNA_...)
  
  37 - 490
  
  HAMAP
  MF_02006 (Tyr_tRNA_synth_type1)
IPR014729

Rossmann-like alpha/beta/alpha sandwich fold
1. G3DSA:3.40.50...
  
  32 - 293
  
  GENE3D
  G3DSA:3.40.50.620 (G3DSA:3.40.50.620)
IPR002942

RNA-binding S4 domain
1. G3DSA:3.10.29...
  
  395 - 492
  
  GENE3D
  G3DSA:3.10.290.10 (G3DSA:3.10.290.10)
IPR001412

Aminoacyl-tRNA synthetase, class I, conserved site
1. PS00178 (AA_TRNA_LI...)
  
  74 - 84
  
  PROSITE patterns
  PS00178 (AA_TRNA_LIGASE_I)

no IPR

Unintegrated signatures

G3DSA:1.10.24...

294 - 394

GENE3D
G3DSA:1.10.240.10 (G3DSA:1.10.240.10)
SSF52374 (Nucleotid...)

39 - 381

SUPERFAMILY
SSF52374 (Nucleotidylyl transferase)

Residue annotation

active site cd00805

69Y

active site
69Y, 71G, 72A, 73D, 80H, 82G, 83N, 217Y, 221Q, 224D, 237Q, 239G, 240G, 243Q, 292L, 293L, 300K, 301F, 302G, 303K
CDD
cd00805 (TyrRS_core)

71G

active site
69Y, 71G, 72A, 73D, 80H, 82G, 83N, 217Y, 221Q, 224D, 237Q, 239G, 240G, 243Q, 292L, 293L, 300K, 301F, 302G, 303K
CDD
cd00805 (TyrRS_core)

72A

active site
69Y, 71G, 72A, 73D, 80H, 82G, 83N, 217Y, 221Q, 224D, 237Q, 239G, 240G, 243Q, 292L, 293L, 300K, 301F, 302G, 303K
CDD
cd00805 (TyrRS_core)

73D

active site
69Y, 71G, 72A, 73D, 80H, 82G, 83N, 217Y, 221Q, 224D, 237Q, 239G, 240G, 243Q, 292L, 293L, 300K, 301F, 302G, 303K
CDD
cd00805 (TyrRS_core)

80H

active site
69Y, 71G, 72A, 73D, 80H, 82G, 83N, 217Y, 221Q, 224D, 237Q, 239G, 240G, 243Q, 292L, 293L, 300K, 301F, 302G, 303K
CDD
cd00805 (TyrRS_core)

82G

active site
69Y, 71G, 72A, 73D, 80H, 82G, 83N, 217Y, 221Q, 224D, 237Q, 239G, 240G, 243Q, 292L, 293L, 300K, 301F, 302G, 303K
CDD
cd00805 (TyrRS_core)

83N

active site
69Y, 71G, 72A, 73D, 80H, 82G, 83N, 217Y, 221Q, 224D, 237Q, 239G, 240G, 243Q, 292L, 293L, 300K, 301F, 302G, 303K
CDD
cd00805 (TyrRS_core)

217Y

active site
69Y, 71G, 72A, 73D, 80H, 82G, 83N, 217Y, 221Q, 224D, 237Q, 239G, 240G, 243Q, 292L, 293L, 300K, 301F, 302G, 303K
CDD
cd00805 (TyrRS_core)

221Q

active site
69Y, 71G, 72A, 73D, 80H, 82G, 83N, 217Y, 221Q, 224D, 237Q, 239G, 240G, 243Q, 292L, 293L, 300K, 301F, 302G, 303K
CDD
cd00805 (TyrRS_core)

224D

active site
69Y, 71G, 72A, 73D, 80H, 82G, 83N, 217Y, 221Q, 224D, 237Q, 239G, 240G, 243Q, 292L, 293L, 300K, 301F, 302G, 303K
CDD
cd00805 (TyrRS_core)

237Q

active site
69Y, 71G, 72A, 73D, 80H, 82G, 83N, 217Y, 221Q, 224D, 237Q, 239G, 240G, 243Q, 292L, 293L, 300K, 301F, 302G, 303K
CDD
cd00805 (TyrRS_core)

239G

active site
69Y, 71G, 72A, 73D, 80H, 82G, 83N, 217Y, 221Q, 224D, 237Q, 239G, 240G, 243Q, 292L, 293L, 300K, 301F, 302G, 303K
CDD
cd00805 (TyrRS_core)

240G

active site
69Y, 71G, 72A, 73D, 80H, 82G, 83N, 217Y, 221Q, 224D, 237Q, 239G, 240G, 243Q, 292L, 293L, 300K, 301F, 302G, 303K
CDD
cd00805 (TyrRS_core)

243Q

active site
69Y, 71G, 72A, 73D, 80H, 82G, 83N, 217Y, 221Q, 224D, 237Q, 239G, 240G, 243Q, 292L, 293L, 300K, 301F, 302G, 303K
CDD
cd00805 (TyrRS_core)

292L

active site
69Y, 71G, 72A, 73D, 80H, 82G, 83N, 217Y, 221Q, 224D, 237Q, 239G, 240G, 243Q, 292L, 293L, 300K, 301F, 302G, 303K
CDD
cd00805 (TyrRS_core)

293L

active site
69Y, 71G, 72A, 73D, 80H, 82G, 83N, 217Y, 221Q, 224D, 237Q, 239G, 240G, 243Q, 292L, 293L, 300K, 301F, 302G, 303K
CDD
cd00805 (TyrRS_core)

300K

active site
69Y, 71G, 72A, 73D, 80H, 82G, 83N, 217Y, 221Q, 224D, 237Q, 239G, 240G, 243Q, 292L, 293L, 300K, 301F, 302G, 303K
CDD
cd00805 (TyrRS_core)

301F

active site
69Y, 71G, 72A, 73D, 80H, 82G, 83N, 217Y, 221Q, 224D, 237Q, 239G, 240G, 243Q, 292L, 293L, 300K, 301F, 302G, 303K
CDD
cd00805 (TyrRS_core)

302G

active site
69Y, 71G, 72A, 73D, 80H, 82G, 83N, 217Y, 221Q, 224D, 237Q, 239G, 240G, 243Q, 292L, 293L, 300K, 301F, 302G, 303K
CDD
cd00805 (TyrRS_core)

303K

active site
69Y, 71G, 72A, 73D, 80H, 82G, 83N, 217Y, 221Q, 224D, 237Q, 239G, 240G, 243Q, 292L, 293L, 300K, 301F, 302G, 303K
CDD
cd00805 (TyrRS_core)
HIGH motif cd00805

80H

HIGH motif
80H, 81V, 82G, 83N
CDD
cd00805 (TyrRS_core)

81V

HIGH motif
80H, 81V, 82G, 83N
CDD
cd00805 (TyrRS_core)

82G

HIGH motif
80H, 81V, 82G, 83N
CDD
cd00805 (TyrRS_core)

83N

HIGH motif
80H, 81V, 82G, 83N
CDD
cd00805 (TyrRS_core)
dimer interface cd...

107A

dimer interface
107A, 110I, 111V, 178M, 179L, 181F, 182L, 184T, 186G, 187R, 189I, 190R, 191V, 192G, 194M, 195L, 213N, 215F, 216T, 219V, 220L
CDD
cd00805 (TyrRS_core)

110I

dimer interface
107A, 110I, 111V, 178M, 179L, 181F, 182L, 184T, 186G, 187R, 189I, 190R, 191V, 192G, 194M, 195L, 213N, 215F, 216T, 219V, 220L
CDD
cd00805 (TyrRS_core)

111V

dimer interface
107A, 110I, 111V, 178M, 179L, 181F, 182L, 184T, 186G, 187R, 189I, 190R, 191V, 192G, 194M, 195L, 213N, 215F, 216T, 219V, 220L
CDD
cd00805 (TyrRS_core)

178M

dimer interface
107A, 110I, 111V, 178M, 179L, 181F, 182L, 184T, 186G, 187R, 189I, 190R, 191V, 192G, 194M, 195L, 213N, 215F, 216T, 219V, 220L
CDD
cd00805 (TyrRS_core)

179L

dimer interface
107A, 110I, 111V, 178M, 179L, 181F, 182L, 184T, 186G, 187R, 189I, 190R, 191V, 192G, 194M, 195L, 213N, 215F, 216T, 219V, 220L
CDD
cd00805 (TyrRS_core)

181F

dimer interface
107A, 110I, 111V, 178M, 179L, 181F, 182L, 184T, 186G, 187R, 189I, 190R, 191V, 192G, 194M, 195L, 213N, 215F, 216T, 219V, 220L
CDD
cd00805 (TyrRS_core)

182L

dimer interface
107A, 110I, 111V, 178M, 179L, 181F, 182L, 184T, 186G, 187R, 189I, 190R, 191V, 192G, 194M, 195L, 213N, 215F, 216T, 219V, 220L
CDD
cd00805 (TyrRS_core)

184T

dimer interface
107A, 110I, 111V, 178M, 179L, 181F, 182L, 184T, 186G, 187R, 189I, 190R, 191V, 192G, 194M, 195L, 213N, 215F, 216T, 219V, 220L
CDD
cd00805 (TyrRS_core)

186G

dimer interface
107A, 110I, 111V, 178M, 179L, 181F, 182L, 184T, 186G, 187R, 189I, 190R, 191V, 192G, 194M, 195L, 213N, 215F, 216T, 219V, 220L
CDD
cd00805 (TyrRS_core)

187R

dimer interface
107A, 110I, 111V, 178M, 179L, 181F, 182L, 184T, 186G, 187R, 189I, 190R, 191V, 192G, 194M, 195L, 213N, 215F, 216T, 219V, 220L
CDD
cd00805 (TyrRS_core)

189I

dimer interface
107A, 110I, 111V, 178M, 179L, 181F, 182L, 184T, 186G, 187R, 189I, 190R, 191V, 192G, 194M, 195L, 213N, 215F, 216T, 219V, 220L
CDD
cd00805 (TyrRS_core)

190R

dimer interface
107A, 110I, 111V, 178M, 179L, 181F, 182L, 184T, 186G, 187R, 189I, 190R, 191V, 192G, 194M, 195L, 213N, 215F, 216T, 219V, 220L
CDD
cd00805 (TyrRS_core)

191V

dimer interface
107A, 110I, 111V, 178M, 179L, 181F, 182L, 184T, 186G, 187R, 189I, 190R, 191V, 192G, 194M, 195L, 213N, 215F, 216T, 219V, 220L
CDD
cd00805 (TyrRS_core)

192G

dimer interface
107A, 110I, 111V, 178M, 179L, 181F, 182L, 184T, 186G, 187R, 189I, 190R, 191V, 192G, 194M, 195L, 213N, 215F, 216T, 219V, 220L
CDD
cd00805 (TyrRS_core)

194M

dimer interface
107A, 110I, 111V, 178M, 179L, 181F, 182L, 184T, 186G, 187R, 189I, 190R, 191V, 192G, 194M, 195L, 213N, 215F, 216T, 219V, 220L
CDD
cd00805 (TyrRS_core)

195L

dimer interface
107A, 110I, 111V, 178M, 179L, 181F, 182L, 184T, 186G, 187R, 189I, 190R, 191V, 192G, 194M, 195L, 213N, 215F, 216T, 219V, 220L
CDD
cd00805 (TyrRS_core)

213N

dimer interface
107A, 110I, 111V, 178M, 179L, 181F, 182L, 184T, 186G, 187R, 189I, 190R, 191V, 192G, 194M, 195L, 213N, 215F, 216T, 219V, 220L
CDD
cd00805 (TyrRS_core)

215F

dimer interface
107A, 110I, 111V, 178M, 179L, 181F, 182L, 184T, 186G, 187R, 189I, 190R, 191V, 192G, 194M, 195L, 213N, 215F, 216T, 219V, 220L
CDD
cd00805 (TyrRS_core)

216T

dimer interface
107A, 110I, 111V, 178M, 179L, 181F, 182L, 184T, 186G, 187R, 189I, 190R, 191V, 192G, 194M, 195L, 213N, 215F, 216T, 219V, 220L
CDD
cd00805 (TyrRS_core)

219V

dimer interface
107A, 110I, 111V, 178M, 179L, 181F, 182L, 184T, 186G, 187R, 189I, 190R, 191V, 192G, 194M, 195L, 213N, 215F, 216T, 219V, 220L
CDD
cd00805 (TyrRS_core)

220L

dimer interface
107A, 110I, 111V, 178M, 179L, 181F, 182L, 184T, 186G, 187R, 189I, 190R, 191V, 192G, 194M, 195L, 213N, 215F, 216T, 219V, 220L
CDD
cd00805 (TyrRS_core)
KMSKS motif cd00805

300K

KMSKS motif
300K, 301F, 302G, 303K, 304S
CDD
cd00805 (TyrRS_core)

301F

KMSKS motif
300K, 301F, 302G, 303K, 304S
CDD
cd00805 (TyrRS_core)

302G

KMSKS motif
300K, 301F, 302G, 303K, 304S
CDD
cd00805 (TyrRS_core)

303K

KMSKS motif
300K, 301F, 302G, 303K, 304S
CDD
cd00805 (TyrRS_core)

304S

KMSKS motif
300K, 301F, 302G, 303K, 304S
CDD
cd00805 (TyrRS_core)

Protein sequence

>MIA_05807_1
MLSFRQFLTPRVSSQVFKLGISTRCYSSENVSSDLKTPLLQHLRDRNLIASVTNESVEKLLETEKVALYCGADPSAQSLH
VGNLIPLIILLHFRARGHTSISLVGGATGIVGDPSGRSTERAQMEADTRANNISKIRGQLETFLERGKQYASTHGYPAER
FGPRVSTNNADWWRDMGMLQFLGTYGRHIRVGQMLARDSVKNRMESEQGIGFNEFTYQVLQAYDFWHLHNTFGCRIQVGG
NDQWGNITAGIDLISRLRSEILKNEKAQSQKSSSLPKVSKESDPAYGITVPLLTTPSGEKFGKSAGNAVWIDPNMTKPYE
LYQYFVKAPDSVVENYLKMFTFISLEKISTIMAQHVENESLRHAQRILAKEVTDFVHGPGSGDNSAVMSEILFGNAESYD
ADTILAAFSSQGLVQQIPKSELVGNSWRSVLASVTGKSKSEISRLLKGKGVYNGLQRKSITEANIEESDFIEGKLLLVRV
GKASYYIIQATN

GO term prediction

Biological Process

GO:0006418 tRNA aminoacylation for protein translation
GO:0006437 tyrosyl-tRNA aminoacylation

Molecular Function

GO:0000166 nucleotide binding
GO:0003723 RNA binding
GO:0004812 aminoacyl-tRNA ligase activity
GO:0004831 tyrosine-tRNA ligase activity
GO:0005524 ATP binding

Cellular Component

GO:0005737 cytoplasm

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Entry type

Status

Per-residue features