MIA_05705_1

Protein

Length

161 amino acids

Protein function

EGGNOG:	0PP5Y	RIM1	Single-stranded DNA-binding protein
SGD closest match:	S000007222	RIM1	Single-stranded DNA-binding protein RIM1, mitochondrial
CGD closest match:	CAL0000182022	RIM1	Rim1p

Protein alignments

	%id	Aln length	E-value
A0A0J9X2H0_GEOCN	51.667%	120	1.18e-38	Similar to Saccharomyces cerevisiae YCR028C-A RIM1 Single-stranded DNA-binding protein essential for mitochondrial genome maintenance OS=Geotrichum candidum GN=BN980_GECA01s01737g PE=4 SV=1
UniRef50_A0A0J9X2H0	51.667%	120	2.42e-35	Similar to Saccharomyces cerevisiae YCR028C-A RIM1 Single-stranded DNA-binding protein essential for mitochondrial genome maintenance n=2 Tax=Saccharomycetales TaxID=4892 RepID=A0A0J9X2H0_GEOCN
MCA_01336_1	44.915%	118	6.70e-34	MCA_01336_1
A0A060T076_BLAAD	47.748%	111	4.47e-32	ARAD1C10626p OS=Blastobotrys adeninivorans GN=GNLVRS02_ARAD1C10626g PE=4 SV=1
A0A1E3PG35_9ASCO	47.664%	107	1.99e-30	Nucleic acid-binding protein OS=Nadsonia fulvescens var. elongata DSM 6958 GN=NADFUDRAFT_47575 PE=4 SV=1
A0A167FMY4_9ASCO	41.912%	136	1.31e-27	Rim1p OS=Sugiyamaella lignohabitans GN=RIM1 PE=4 SV=1
RIM1_YEAST	41.964%	112	1.01e-24	Single-stranded DNA-binding protein RIM1, mitochondrial OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=RIM1 PE=1 SV=1
A0A1E4TFS6_9ASCO	39.091%	110	6.30e-15	Uncharacterized protein OS=Tortispora caseinolytica NRRL Y-17796 GN=CANCADRAFT_31471 PE=4 SV=1
Q5AA01_CANAL	32.110%	109	1.15e-13	Rim1p OS=Candida albicans (strain SC5314 / ATCC MYA-2876) GN=RIM1 PE=4 SV=1

Mitochondrial localization by mitoprotII

Probability of mitochondrial location: 0.9160
Predicted cleavage: 46

Protein family membership

Primosome PriB/single-strand DNA-binding (IPR000424)

Domains and repeats

Domain
36 - 147
Nucleic acid-binding, OB-fold (IPR012340)

1 20 40 60 80 100 120 140 161

Detailed signature matches

IPR000424

Primosome PriB/single-strand DNA-binding
1. PS50935 (SSB)
  
  34 - 133
  
  PROSITE profiles
  PS50935 (SSB)
2. PF00436 (SSB)
  
  37 - 130
  
  Pfam
  PF00436 (SSB)
3. cd04496 (SSB_OBF)
  
  39 - 132
  
  CDD
  cd04496 (SSB_OBF)
IPR012340

Nucleic acid-binding, OB-fold
1. SSF50249 (Nucleic a...)
  
  36 - 147
  
  SUPERFAMILY
  SSF50249 (Nucleic acid-binding proteins)

no IPR

Unintegrated signatures

G3DSA:2.40.50...

22 - 140

GENE3D
G3DSA:2.40.50.140 (G3DSA:2.40.50.140)
PIRSF002070 (SSB)

33 - 161

PIRSF
PIRSF002070 (SSB)
mobidb-lite (disord...)

133 - 161

MobiDB Lite
mobidb-lite (disorder_prediction)

Residue annotation

dimer interface cd...

39M

dimer interface
39M, 40T, 41L, 42V, 67V, 68N, 69T, 75S, 77F, 99L, 108Q, 119I, 122L
CDD
cd04496 (SSB_OBF)

40T

dimer interface
39M, 40T, 41L, 42V, 67V, 68N, 69T, 75S, 77F, 99L, 108Q, 119I, 122L
CDD
cd04496 (SSB_OBF)

41L

dimer interface
39M, 40T, 41L, 42V, 67V, 68N, 69T, 75S, 77F, 99L, 108Q, 119I, 122L
CDD
cd04496 (SSB_OBF)

42V

dimer interface
39M, 40T, 41L, 42V, 67V, 68N, 69T, 75S, 77F, 99L, 108Q, 119I, 122L
CDD
cd04496 (SSB_OBF)

67V

dimer interface
39M, 40T, 41L, 42V, 67V, 68N, 69T, 75S, 77F, 99L, 108Q, 119I, 122L
CDD
cd04496 (SSB_OBF)

68N

dimer interface
39M, 40T, 41L, 42V, 67V, 68N, 69T, 75S, 77F, 99L, 108Q, 119I, 122L
CDD
cd04496 (SSB_OBF)

69T

dimer interface
39M, 40T, 41L, 42V, 67V, 68N, 69T, 75S, 77F, 99L, 108Q, 119I, 122L
CDD
cd04496 (SSB_OBF)

75S

dimer interface
39M, 40T, 41L, 42V, 67V, 68N, 69T, 75S, 77F, 99L, 108Q, 119I, 122L
CDD
cd04496 (SSB_OBF)

77F

dimer interface
39M, 40T, 41L, 42V, 67V, 68N, 69T, 75S, 77F, 99L, 108Q, 119I, 122L
CDD
cd04496 (SSB_OBF)

99L

dimer interface
39M, 40T, 41L, 42V, 67V, 68N, 69T, 75S, 77F, 99L, 108Q, 119I, 122L
CDD
cd04496 (SSB_OBF)

108Q

dimer interface
39M, 40T, 41L, 42V, 67V, 68N, 69T, 75S, 77F, 99L, 108Q, 119I, 122L
CDD
cd04496 (SSB_OBF)

119I

dimer interface
39M, 40T, 41L, 42V, 67V, 68N, 69T, 75S, 77F, 99L, 108Q, 119I, 122L
CDD
cd04496 (SSB_OBF)

122L

dimer interface
39M, 40T, 41L, 42V, 67V, 68N, 69T, 75S, 77F, 99L, 108Q, 119I, 122L
CDD
cd04496 (SSB_OBF)
ssDNA binding site...

44R

ssDNA binding site
44R, 45V, 46G, 52N, 62Q, 69T, 70S, 73H, 74T, 76W, 78K, 80V, 82F, 96K, 97G, 105S, 107S, 109Q, 110V, 123Q, 125V
CDD
cd04496 (SSB_OBF)

45V

ssDNA binding site
44R, 45V, 46G, 52N, 62Q, 69T, 70S, 73H, 74T, 76W, 78K, 80V, 82F, 96K, 97G, 105S, 107S, 109Q, 110V, 123Q, 125V
CDD
cd04496 (SSB_OBF)

46G

ssDNA binding site
44R, 45V, 46G, 52N, 62Q, 69T, 70S, 73H, 74T, 76W, 78K, 80V, 82F, 96K, 97G, 105S, 107S, 109Q, 110V, 123Q, 125V
CDD
cd04496 (SSB_OBF)

52N

ssDNA binding site
44R, 45V, 46G, 52N, 62Q, 69T, 70S, 73H, 74T, 76W, 78K, 80V, 82F, 96K, 97G, 105S, 107S, 109Q, 110V, 123Q, 125V
CDD
cd04496 (SSB_OBF)

62Q

ssDNA binding site
44R, 45V, 46G, 52N, 62Q, 69T, 70S, 73H, 74T, 76W, 78K, 80V, 82F, 96K, 97G, 105S, 107S, 109Q, 110V, 123Q, 125V
CDD
cd04496 (SSB_OBF)

69T

ssDNA binding site
44R, 45V, 46G, 52N, 62Q, 69T, 70S, 73H, 74T, 76W, 78K, 80V, 82F, 96K, 97G, 105S, 107S, 109Q, 110V, 123Q, 125V
CDD
cd04496 (SSB_OBF)

70S

ssDNA binding site
44R, 45V, 46G, 52N, 62Q, 69T, 70S, 73H, 74T, 76W, 78K, 80V, 82F, 96K, 97G, 105S, 107S, 109Q, 110V, 123Q, 125V
CDD
cd04496 (SSB_OBF)

73H

ssDNA binding site
44R, 45V, 46G, 52N, 62Q, 69T, 70S, 73H, 74T, 76W, 78K, 80V, 82F, 96K, 97G, 105S, 107S, 109Q, 110V, 123Q, 125V
CDD
cd04496 (SSB_OBF)

74T

ssDNA binding site
44R, 45V, 46G, 52N, 62Q, 69T, 70S, 73H, 74T, 76W, 78K, 80V, 82F, 96K, 97G, 105S, 107S, 109Q, 110V, 123Q, 125V
CDD
cd04496 (SSB_OBF)

76W

ssDNA binding site
44R, 45V, 46G, 52N, 62Q, 69T, 70S, 73H, 74T, 76W, 78K, 80V, 82F, 96K, 97G, 105S, 107S, 109Q, 110V, 123Q, 125V
CDD
cd04496 (SSB_OBF)

78K

ssDNA binding site
44R, 45V, 46G, 52N, 62Q, 69T, 70S, 73H, 74T, 76W, 78K, 80V, 82F, 96K, 97G, 105S, 107S, 109Q, 110V, 123Q, 125V
CDD
cd04496 (SSB_OBF)

80V

ssDNA binding site
44R, 45V, 46G, 52N, 62Q, 69T, 70S, 73H, 74T, 76W, 78K, 80V, 82F, 96K, 97G, 105S, 107S, 109Q, 110V, 123Q, 125V
CDD
cd04496 (SSB_OBF)

82F

ssDNA binding site
44R, 45V, 46G, 52N, 62Q, 69T, 70S, 73H, 74T, 76W, 78K, 80V, 82F, 96K, 97G, 105S, 107S, 109Q, 110V, 123Q, 125V
CDD
cd04496 (SSB_OBF)

96K

ssDNA binding site
44R, 45V, 46G, 52N, 62Q, 69T, 70S, 73H, 74T, 76W, 78K, 80V, 82F, 96K, 97G, 105S, 107S, 109Q, 110V, 123Q, 125V
CDD
cd04496 (SSB_OBF)

97G

ssDNA binding site
44R, 45V, 46G, 52N, 62Q, 69T, 70S, 73H, 74T, 76W, 78K, 80V, 82F, 96K, 97G, 105S, 107S, 109Q, 110V, 123Q, 125V
CDD
cd04496 (SSB_OBF)

105S

ssDNA binding site
44R, 45V, 46G, 52N, 62Q, 69T, 70S, 73H, 74T, 76W, 78K, 80V, 82F, 96K, 97G, 105S, 107S, 109Q, 110V, 123Q, 125V
CDD
cd04496 (SSB_OBF)

107S

ssDNA binding site
44R, 45V, 46G, 52N, 62Q, 69T, 70S, 73H, 74T, 76W, 78K, 80V, 82F, 96K, 97G, 105S, 107S, 109Q, 110V, 123Q, 125V
CDD
cd04496 (SSB_OBF)

109Q

ssDNA binding site
44R, 45V, 46G, 52N, 62Q, 69T, 70S, 73H, 74T, 76W, 78K, 80V, 82F, 96K, 97G, 105S, 107S, 109Q, 110V, 123Q, 125V
CDD
cd04496 (SSB_OBF)

110V

ssDNA binding site
44R, 45V, 46G, 52N, 62Q, 69T, 70S, 73H, 74T, 76W, 78K, 80V, 82F, 96K, 97G, 105S, 107S, 109Q, 110V, 123Q, 125V
CDD
cd04496 (SSB_OBF)

123Q

ssDNA binding site
44R, 45V, 46G, 52N, 62Q, 69T, 70S, 73H, 74T, 76W, 78K, 80V, 82F, 96K, 97G, 105S, 107S, 109Q, 110V, 123Q, 125V
CDD
cd04496 (SSB_OBF)

125V

ssDNA binding site
44R, 45V, 46G, 52N, 62Q, 69T, 70S, 73H, 74T, 76W, 78K, 80V, 82F, 96K, 97G, 105S, 107S, 109Q, 110V, 123Q, 125V
CDD
cd04496 (SSB_OBF)
tetramer (dimer of...

99L

tetramer (dimer of dimers) interface
99L, 101Y, 103E, 130T
CDD
cd04496 (SSB_OBF)

101Y

tetramer (dimer of dimers) interface
99L, 101Y, 103E, 130T
CDD
cd04496 (SSB_OBF)

103E

tetramer (dimer of dimers) interface
99L, 101Y, 103E, 130T
CDD
cd04496 (SSB_OBF)

130T

tetramer (dimer of dimers) interface
99L, 101Y, 103E, 130T
CDD
cd04496 (SSB_OBF)

Protein sequence

>MIA_05705_1
MNFFRNLSSIPRASSNFAGMASARAFSTTPVARSNVARMTLVGRVGSDIAVNNSKNNRSYIQYSLAVNTSKDHTSWFKIV
VFDPNQLQFMENYVRKGNLVYVEASASQQVYTAENERKITTLQLVQQSITKIKTKAPSSEEGEAVENAEAVEGSEEQQPH
A

GO term prediction

Biological Process

None predicted.

Molecular Function

GO:0003697 single-stranded DNA binding

Cellular Component