Protein

MIA_05678_1

Length
144 amino acids

Browser: contig09:45905-46340-

Protein function

EGGNOG:0PRABATG12Ubiquitin-like protein involved in cytoplasm to vacuole transport (Cvt), autophagy vesicles formation, mitophagy, and nucleophagy. Conjugation with atg5 through an ubiquitin-like conjugating system involving also atg7 as an E1-like activating enzyme and atg10 as an E2-like conjugating enzyme, is essential for its function. The atg12-atg5 conjugate functions as an E3-like enzyme which is required for lipidation of atg8 and atg8 association to the vesicle membranes
SGD closest match:S000000421ATG12Ubiquitin-like protein ATG12
CGD closest match:CAL0000188918ATG12Ubiquitin-like protein ATG12

Protein alignments

%idAln lengthE-value
MCA_01982_167.816%871.52e-43MCA_01982_1
A0A0J9X4W1_GEOCN66.667%873.92e-38Ubiquitin-like protein ATG12 OS=Geotrichum candidum GN=BN980_GECA03s02540g PE=3 SV=1
UniRef50_Q2UMW651.724%872.43e-24Ubiquitin-like protein ATG12 n=17 Tax=leotiomyceta TaxID=716546 RepID=ATG12_ASPOR
ATG12_YARLI56.667%901.70e-24Ubiquitin-like protein ATG12 OS=Yarrowia lipolytica (strain CLIB 122 / E 150) GN=ATG12 PE=3 SV=1
ATG12_YEAST45.977%874.37e-22Ubiquitin-like protein ATG12 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=ATG12 PE=1 SV=1
A0A1E3PLF2_9ASCO40.426%941.50e-18Ubiquitin-like protein ATG12 (Fragment) OS=Nadsonia fulvescens var. elongata DSM 6958 GN=NADFUDRAFT_11512 PE=3 SV=1
ATG12_CANAL44.828%872.77e-16Ubiquitin-like protein ATG12 OS=Candida albicans (strain SC5314 / ATCC MYA-2876) GN=ATG12 PE=3 SV=1

Mitochondrial localization by mitoprotII

Probability of mitochondrial location: 0.0130

Protein family membership

Domains and repeats

  1. Domain
1 20 40 60 80 100 120 144

Detailed signature matches

  1. Family IPR007242
    Ubiquitin-like protein Atg12
    1. PF04110 (APG12)
    2. cd01612 (APG12_C)
  2. Domain IPR029071
    Ubiquitin-related domain
    1. SSF54236 (Ubiquitin...)
Unintegrated signatures no IPR
Unintegrated signatures
  1. G3DSA:3.10.20.90
  2. mobidb-lite (disord...)

Protein sequence

>MIA_05678_1
MSSTAVQIPDDDDSDNQRAGPPPVSLSASVVLAALPADSSQLLSRTAAAAASTAAATKITLRLRPIGSAAPLKQSVYRIS
GGQTFAGLVKFLRKQLKCPPSQSIFCYINNSFAPPLDEPLINLYHNFSVDDHLIISYCYTVAFG

GO term prediction

Biological Process

GO:0000045 autophagosome assembly

Molecular Function

None predicted.

Cellular Component

GO:0005737 cytoplasm