Protein

MIA_05652_1

Length
329 amino acids

Browser: contig08:1007981-1008971+

Protein function

EGGNOG:0PG5BHydroxymethylglutaryl-CoA lyase
SGD closest match:S000002341LYS20Homocitrate synthase, cytosolic isozyme
CGD closest match:CAL0000190246LYS21Homocitrate synthase

Protein alignments

%idAln lengthE-value
A0A0J9XGB3_GEOCN71.523%3021.98e-151Uncharacterized protein OS=Geotrichum candidum GN=BN980_GECA13s00648g PE=4 SV=1
MCA_03189_169.079%3047.82e-142MCA_03189_1
A0A060T3U2_BLAAD65.347%3032.67e-135ARAD1C42812p OS=Blastobotrys adeninivorans GN=GNLVRS02_ARAD1C42812g PE=4 SV=1
A0A167FIV1_9ASCO65.461%3047.69e-135Uncharacterized protein OS=Sugiyamaella lignohabitans GN=AWJ20_2986 PE=4 SV=1
Q6CDN6_YARLI61.462%3011.12e-128YALI0B22550p OS=Yarrowia lipolytica (strain CLIB 122 / E 150) GN=YALI0_B22550g PE=4 SV=1
A0A1E4T9F9_9ASCO58.007%2811.30e-115Uncharacterized protein OS=Tortispora caseinolytica NRRL Y-17796 GN=CANCADRAFT_4552 PE=4 SV=1
UniRef50_E0VUT154.122%2791.13e-107Hydroxymethylglutaryl-CoA lyase, putative n=41 Tax=cellular organisms TaxID=131567 RepID=E0VUT1_PEDHC
A0A1E3PFI7_9ASCO49.677%3101.19e-107Hydroxymethylglutaryl-CoA lyase OS=Nadsonia fulvescens var. elongata DSM 6958 GN=NADFUDRAFT_47465 PE=4 SV=1
HOSC_YEAST23.171%2461.09e-06Homocitrate synthase, cytosolic isozyme OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=LYS20 PE=1 SV=1
Q59VG1_CANAL22.764%2462.91e-06Homocitrate synthase OS=Candida albicans (strain SC5314 / ATCC MYA-2876) GN=LYS21 PE=3 SV=1

Mitochondrial localization by mitoprotII

Probability of mitochondrial location: 0.9512
Predicted cleavage: 30

Protein family membership

None predicted.

Domains and repeats

  1. Domain
1 50 100 150 200 250 300 329

Detailed signature matches

  1. Domain IPR013785
    Aldolase-type TIM barrel
    1. G3DSA:3.20.20.70
  2. Domain IPR000891
    Pyruvate carboxyltransferase
    1. PF00682 (HMGL-like)
    2. PS50991 (PYR_CT)
  3. SITE IPR000138
    Hydroxymethylglutaryl-CoA lyase, active site
    1. PS01062 (HMG_COA_LYASE)
Unintegrated signatures no IPR
Unintegrated signatures
  1. SSF51569 (Aldolase)
  2. cd07938 (DRE_TIM_HMGL)

Residue annotation

  1. catalytic residues...
  2. active site cd07938
  3. metal binding site...

Protein sequence

>MIA_05652_1
MMKVNNRGIHNFFKVVPQFRSFSSNSQNFVKIVDVSPRDGLQNEPTIVPTSVKLELIDRLTKANVPSIEITSFVSPKWVP
QMADANDVLRAVQSKYEGNEISYPILIPNTKGLERALETGVPIQEIAIFGAASEGFSKKNTNTSVDEGIKVLKEVANLAH
SKGIKVRGYLSTVIGCPYDGPTDPKRVAELSQQYLEEIGCYELSLGDTIGVGTIKTTENMLTEVFKKVHPSKIAIHAHDT
YGQGVANVLKAVEMGVRVVDASVAGLGGCPYARGATGNVSTEDIVYALHNSGYQTGIDLISLSKTGEWISSSINRSNASR
AGKAIFSKL

GO term prediction

Biological Process

None predicted.

Molecular Function

GO:0003824 catalytic activity
GO:0004419 hydroxymethylglutaryl-CoA lyase activity

Cellular Component

None predicted.