Protein

MIA_05646_1

Length
581 amino acids

Browser: contig08:997097-998866-

Protein function

EGGNOG:0PFDFPRP28ATP-dependent RNA helicase involved in mRNA splicing. May destabilize the U1 5'-splice site duplex to permit an effective competition for the 5'-splice site by the U6 snRNA, resulting in the switch between U1 and U6 at the 5'-splice site. May also act to unwind the U4 U6 base-pairing interaction in the U4 U6 U5 snRNP, facilitating the first covalent step of splicing
SGD closest match:S000002651PRP28Pre-mRNA-splicing ATP-dependent RNA helicase PRP28
CGD closest match:CAL0000181927PRP28Pre-mRNA-splicing ATP-dependent RNA helicase PRP28

Protein alignments

%idAln lengthE-value
A0A0J9X8F2_GEOCN56.847%6280.0Similar to Saccharomyces cerevisiae YDR243C PRP28 RNA helicase in the DEAD-box family OS=Geotrichum candidum GN=BN980_GECA05s02265g PE=3 SV=1
A0A060TJI5_BLAAD55.842%5820.0ARAD1D47036p OS=Blastobotrys adeninivorans GN=GNLVRS02_ARAD1D47036g PE=3 SV=1
MCA_03324_152.581%6200.0MCA_03324_1
A0A167DKT1_9ASCO53.535%5940.0Prp28p OS=Sugiyamaella lignohabitans GN=PRP28 PE=3 SV=1
UniRef50_A0A167DKT153.535%5940.0Prp28p n=3 Tax=Opisthokonta TaxID=33154 RepID=A0A167DKT1_9ASCO
A0A1E3PQ74_9ASCO54.991%5310.0DEAD-domain-containing protein OS=Nadsonia fulvescens var. elongata DSM 6958 GN=NADFUDRAFT_82027 PE=3 SV=1
PRP28_YARLI48.705%5790.0Pre-mRNA-splicing ATP-dependent RNA helicase PRP28 OS=Yarrowia lipolytica (strain CLIB 122 / E 150) GN=PRP28 PE=3 SV=1
A0A1E4TG60_9ASCO54.916%4173.23e-164Uncharacterized protein (Fragment) OS=Tortispora caseinolytica NRRL Y-17796 GN=CANCADRAFT_11392 PE=3 SV=1
PRP28_CANAL47.867%4228.54e-137Pre-mRNA-splicing ATP-dependent RNA helicase PRP28 OS=Candida albicans (strain SC5314 / ATCC MYA-2876) GN=PRP28 PE=3 SV=2
PRP28_YEAST39.425%5913.38e-123Pre-mRNA-splicing ATP-dependent RNA helicase PRP28 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=PRP28 PE=1 SV=2

Mitochondrial localization by mitoprotII

Probability of mitochondrial location: 0.1230

Protein family membership

None predicted.

Domains and repeats

  1. Domain
  2. Domain
  3. Domain
1 100 200 300 400 500 581

Detailed signature matches

  1. Domain IPR027417
    P-loop containing nucleoside triphosphate hydrolase
    1. SSF52540 (P-loop co...)
  2. Domain IPR014014
    RNA helicase, DEAD-box type, Q motif
    1. PS51195 (Q_MOTIF)
  3. Domain IPR014001
    Helicase superfamily 1/2, ATP-binding domain
    1. PS51192 (HELICASE_A...)
    2. SM00487 (ultradead3)
  4. Domain IPR011545
    DEAD/DEAH box helicase domain
    1. PF00270 (DEAD)
  5. Domain IPR001650
    Helicase, C-terminal
    1. PS51194 (HELICASE_CTER)
    2. SM00490 (helicmild6)
    3. PF00271 (Helicase_C)
  6. SITE IPR000629
    ATP-dependent RNA helicase DEAD-box, conserved site
    1. PS00039 (DEAD_ATP_H...)
Unintegrated signatures no IPR
Unintegrated signatures
  1. G3DSA:3.40.50...
  2. cd00079 (HELICc)
  3. cd00268 (DEADc)
  4. mobidb-lite (disord...)

Residue annotation

  1. ATP binding site c...
  2. Mg++ binding site ...
  3. motif III cd00268
  4. nucleotide binding...
  5. ATP-binding site c...

Protein sequence

>MIA_05646_1
MSSSNKITPISINELIKKKKEESERQKFPVFLSKTQRDELARERSNISCNKDKKTARPDFLQQSGNAKIPASHINLIKPN
LNKKTLAPKPGQPKKKETKFKFEWNSQDDTLDLNDPLYFSKSKIGSNEKLENEITKRHNKLTSGFNLRNTKRLKVDYNEL
HWSEKPLGEMKERDWRIFREDFDILTKGGNIPHPIRFWDESDIPSELLKIIHILGYKEPTPIQRAAIPIALSGRDVIGIA
ETGSGKTASFVIPLLASIMKFPILNELTKRDGPYAIILAPTRELAQQIETETKKFLKPLEFTCMSIVGGHSIEEQIHKLN
LGAEIIIATPGRLLDTLDRKILVLSQCRYVVMDEADRMIDLGFEEQVNGILNALPSDKEANFDNMSISNFKGKSWQTMMY
TATWPRAIERLSEKYLSAPGVVTIGNTGQVTERVEQRTEFIGSEEKRLQRLLKILNSGTFKPPIIIFVNYKRNCENLAQE
LNYHKWRTVTMHGSKSQDQREASLAQLRSGEADCLVATDVAGRGIDISNVSLVVNFQMAKTIENYTHRIGRTGRAGMSGV
AITFLGPEDEEVIPHLKRSSI

GO term prediction

Biological Process

None predicted.

Molecular Function

GO:0003676 nucleic acid binding
GO:0005524 ATP binding

Cellular Component

None predicted.