Protein
MCA_03324_1
Length
619 amino acids
Gene name: PRP28
Description: Pre-mRNA-splicing ATP-dependent RNA helicase PRP28
Browser: contigB:4022593-4024453+
RNA-seq: read pairs 1100, FPKM 21.9, percentile rank 43.8% (100% = highest expression)
Protein function
Annotation: | PRP28 | Pre-mRNA-splicing ATP-dependent RNA helicase PRP28 | |
---|---|---|---|
KEGG: | K12858 | DDX23 | ATP-dependent RNA helicase DDX23/PRP28 [EC:3.6.4.13] |
EGGNOG: | 0PFDF | PRP28 | ATP-dependent RNA helicase involved in mRNA splicing. May destabilize the U1 5'-splice site duplex to permit an effective competition for the 5'-splice site by the U6 snRNA, resulting in the switch between U1 and U6 at the 5'-splice site. May also act to unwind the U4 U6 base-pairing interaction in the U4 U6 U5 snRNP, facilitating the first covalent step of splicing |
SGD closest match: | S000002651 | PRP28 | Pre-mRNA-splicing ATP-dependent RNA helicase PRP28 |
CGD closest match: | CAL0000181927 | PRP28 | Pre-mRNA-splicing ATP-dependent RNA helicase PRP28 |
Protein alignments
%id | Aln length | E-value | ||
---|---|---|---|---|
A0A0J9X8F2_GEOCN | 56.74% | 571 | 0.0 | Similar to Saccharomyces cerevisiae YDR243C PRP28 RNA helicase in the DEAD-box family OS=Geotrichum candidum GN=BN980_GECA05s02265g PE=3 SV=1 |
A0A167DKT1_9ASCO | 48.62% | 652 | 0.0 | Prp28p OS=Sugiyamaella lignohabitans GN=PRP28 PE=3 SV=1 |
UniRef50_A0A167DKT1 | 48.62% | 652 | 0.0 | Prp28p n=3 Tax=Opisthokonta TaxID=33154 RepID=A0A167DKT1_9ASCO |
MIA_05646_1 | 51.45% | 620 | 0.0 | MIA_05646_1 |
A0A060TJI5_BLAAD | 49.92% | 649 | 0.0 | ARAD1D47036p OS=Blastobotrys adeninivorans GN=GNLVRS02_ARAD1D47036g PE=3 SV=1 |
A0A1E3PQ74_9ASCO | 47.15% | 596 | 1e-174 | DEAD-domain-containing protein OS=Nadsonia fulvescens var. elongata DSM 6958 GN=NADFUDRAFT_82027 PE=3 SV=1 |
PRP28_YARLI | 45.30% | 627 | 1e-171 | Pre-mRNA-splicing ATP-dependent RNA helicase PRP28 OS=Yarrowia lipolytica (strain CLIB 122 / E 150) GN=PRP28 PE=3 SV=1 |
A0A1E4TG60_9ASCO | 50.63% | 476 | 1e-153 | Uncharacterized protein (Fragment) OS=Tortispora caseinolytica NRRL Y-17796 GN=CANCADRAFT_11392 PE=3 SV=1 |
PRP28_CANAL | 40.87% | 531 | 2e-130 | Pre-mRNA-splicing ATP-dependent RNA helicase PRP28 OS=Candida albicans (strain SC5314 / ATCC MYA-2876) GN=PRP28 PE=3 SV=2 |
PRP28_YEAST | 39.00% | 541 | 8e-117 | Pre-mRNA-splicing ATP-dependent RNA helicase PRP28 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=PRP28 PE=1 SV=2 |
Mitochondrial localization by mitoprotII
Probability of mitochondrial location: 0.1636
Protein family membership
None predicted.
Domains and repeats
-
Domain
-
Domain
-
Domain
1
100
200
300
400
500
619
Detailed signature matches
no IPR
Unintegrated signatures
-
-
cd00079 (HELICc)
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cd00268 (DEADc)
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mobidb-lite (disord...)
Residue annotation
-
ATP binding site c...
-
Mg++ binding site ...
-
motif III cd00268
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nucleotide binding...
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ATP-binding site c...
Protein sequence
>MCA_03324_1 MSRSTPISVEELLRKKKEEANAQKQPRFLTKAQREALKAASSSTAKVTKSASSSIINKPRLPKPIEDKQNEKPITKQKKA PSQQRHDKFRFDWGENEDTLDKNDPLYALASTSSTITNSRNEYNNPRNSKASNGAIDWDDVHWSKKPIEKMKERDWRIFR EDFSIAIKGAVISSKSDEDNKNAKKTAPKPIRYWKESSIPSQILDIVHELRYSQPTPIQRAAIPFALAGRDVIGVAETGS GKTASFVIPLLATILDIQQSNYEYNAKNDGPLAVILAPTRELAQQIEGETMKFCTPLGLRCSSIVGGHALEEQVHYLQQG VEIIIATPGRLVDLIERKAIALGECRYVVLDEADRMIDLGFEDQVNAILSALPKTTMASGSTRQTMMYTATWPRAIERLA ENYLRHPAIVTIGTAGQATDRVEQRVMFIQSEERRAETLCTILRNGLPSNSNSLPDSEKSGRSDSHRRNRSNQNDLPFPP PIIVFVNYKRTCDFVVKKLNSEGWRTSIMHGSRSQEQREAALASLRDGHSSILVATDVAGRGIDVPNVSLVVNFQMAKNI EDYTHRIGRTGRAGKYGVAVTFLGRDEDVNVLTDLKSMIEKSPISKVPDELRRLTVKKF
GO term prediction
Biological Process
None predicted.
Molecular Function
GO:0003676 nucleic acid binding
GO:0005524 ATP binding
Cellular Component
None predicted.