Protein

MIA_05544_1

Length
769 amino acids

Browser: contig08:776861-779221-

Protein function

EGGNOG:0PFYDFG09744.1tRNA (Cytosine-5-)-methyltransferase
SGD closest match:S000000120NCL1Multisite-specific tRNA:(cytosine-C(5))-methyltransferase
CGD closest match:CAL0000192993CAALFM_CR04300WAtRNA (Cytosine-5-)-methyltransferase

Protein alignments

%idAln lengthE-value
MCA_02073_164.324%7400.0MCA_02073_1
A0A060T8Y2_BLAAD48.564%7310.0ARAD1D11154p OS=Blastobotrys adeninivorans GN=GNLVRS02_ARAD1D11154g PE=3 SV=1
A0A161HMS9_9ASCO47.749%7330.0Ncl1p OS=Sugiyamaella lignohabitans GN=NCL1 PE=3 SV=1
A0A0J9X5A0_GEOCN47.303%7230.0Similar to Saccharomyces cerevisiae YBL024W NCL1 S-adenosyl-L-methionine-dependent tRNA: m5C-methyltransferase OS=Geotrichum candidum GN=BN980_GECA02s09063g PE=3 SV=1
A0A1E3PQQ8_9ASCO48.232%7070.0S-adenosyl-L-methionine-dependent methyltransferase OS=Nadsonia fulvescens var. elongata DSM 6958 GN=NADFUDRAFT_20543 PE=3 SV=1
Q6C6S1_YARLI47.862%7250.0YALI0E06765p OS=Yarrowia lipolytica (strain CLIB 122 / E 150) GN=YALI0_E06765g PE=3 SV=1
UniRef50_Q6C6S147.862%7250.0YALI0E06765p n=3 Tax=Saccharomycetales TaxID=4892 RepID=Q6C6S1_YARLI
A0A1D8PSR2_CANAL44.711%7090.0tRNA (Cytosine-5-)-methyltransferase OS=Candida albicans (strain SC5314 / ATCC MYA-2876) GN=CAALFM_CR04300WA PE=3 SV=1
NCL1_YEAST44.789%7100.0Multisite-specific tRNA:(cytosine-C(5))-methyltransferase OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=NCL1 PE=1 SV=1
A0A1E4TKQ4_9ASCO45.137%6580.0Uncharacterized protein OS=Tortispora caseinolytica NRRL Y-17796 GN=CANCADRAFT_84943 PE=3 SV=1

Mitochondrial localization by mitoprotII

Probability of mitochondrial location: 0.6587
Predicted cleavage: 13

Protein family membership

Domains and repeats

  1. Domain
1 100 200 300 400 500 600 700 769

Detailed signature matches

  1. Family IPR023267
    RNA (C5-cytosine) methyltransferase
    1. PR02008 (RCMTFAMILY)
  2. Family IPR023270
    tRNA (C5-cytosine) methyltransferase, NCL1
    1. PR02011 (RCMTNCL1)
  3. Domain IPR029063
    S-adenosyl-L-methionine-dependent methyltransferase
    1. SSF53335 (S-adenosy...)
  4. Domain IPR001678
    SAM-dependent methyltransferase RsmB/NOP2-type
    1. PF01189 (Methyltr_R...)
    2. PS51686 (SAM_MT_RSM...)
  5. SITE IPR018314
    Bacterial Fmu (Sun)/eukaryotic nucleolar NOL1/Nop2p, conserved site
    1. PS01153 (NOL1_NOP2_SUN)
Unintegrated signatures no IPR
Unintegrated signatures
  1. Coil
  2. G3DSA:3.40.50...
  3. cd02440 (AdoMet_MTases)
  4. mobidb-lite (disord...)

Residue annotation

  1. S-adenosylmethioni...

Protein sequence

>MIA_05544_1
MGKKNMRSRARTSGNWESAVKENEKWEAYYKGQQNLFTDQADFDQFKKACQQPLPLTFRITSSAPACAEVQQLIENDYVK
HTQNIVFNGENIPPPKPLPFYTAKGLAWQVDVGKQVIRRSKEFSRLQRFLVVETDAGNIYRQEAVSMIPPLFLDVQPDNA
VIDLCAAPGSKTGQLIEALHRDGPSSVPSGFVLANDSDYKRAHMLIHQIKRLNSPNLVVTNHDAQMFPKMKTASGDVLKF
DRVLCDVPCSGDATMRKNINVWTDWTIGNGNGLHATQINILLRGIQLLKPGGRLVYSTCSLNPIENEAVVAEALRRCGSS
LALLDVSSNLPELVRRPGVSTWRVQNKAGQWVDPPSNESASSSDDPKPAVTHTSKHLLPSFFPPSEAEAKLFGLDKCVRV
YPHLQNTGGFFIAVFEKLDTKKRPAELDQQDVKKPKSEETPAAEEIIAEKPVDTPAEETTAEKPVDAPAEETTAEKPADT
PAAEKPADSAATAPKEQIPVSSQTDEEVFRFIPSSNELLKSIWDFYKFNTTTFPGGRNTLLVRNSTGIPSRAIYYVSPSV
KPLLELNDQKVKFVHAGLRMFSKQRSPDSPCPWRIHAEAISIVAPHVARDGSGRFHVAKDINTLRLLVTKEFPNFAAVKE
VDEDLAAFIEHLPEGTFILEIPRIGQTSTLVSQSVLDVPLLYPVWRGKTSVNLMLAKQDLAELVHRVFGEENIKTRETNN
NGAGVKKAKPEEKPSEAEDKAEEKAEEKAEEKAEEKTEEKTEEKTEEKN

GO term prediction

Biological Process

None predicted.

Molecular Function

GO:0003723 RNA binding
GO:0008168 methyltransferase activity
GO:0016428 tRNA (cytosine-5-)-methyltransferase activity

Cellular Component

None predicted.