Protein

MCA_02073_1

Length
780 amino acids

Gene name: NCL1

Description: Multisite-specific tRNA:(cytosine-C(5))-methyltransferase

Browser: contigB:157493-159836-

RNA-seq: read pairs 2801, FPKM 44.3, percentile rank 62.6% (100% = highest expression)

Protein function

Annotation:NCL1Multisite-specific tRNA:(cytosine-C(5))-methyltransferase
KEGG:K15334NCL1 multisite-specific tRNA:(cytosine-C5)-methyltransferase [EC:2.1.1.202]
EGGNOG:0PFYDFG09744.1tRNA (Cytosine-5-)-methyltransferase
SGD closest match:S000000120NCL1Multisite-specific tRNA:(cytosine-C(5))-methyltransferase
CGD closest match:CAL0000192993CAALFM_CR04300WAtRNA (Cytosine-5-)-methyltransferase

Protein alignments

%idAln lengthE-value
MIA_05544_164.49%7350.0MIA_05544_1
A0A060T8Y2_BLAAD50.56%7120.0ARAD1D11154p OS=Blastobotrys adeninivorans GN=GNLVRS02_ARAD1D11154g PE=3 SV=1
A0A0J9X5A0_GEOCN48.60%7140.0Similar to Saccharomyces cerevisiae YBL024W NCL1 S-adenosyl-L-methionine-dependent tRNA: m5C-methyltransferase OS=Geotrichum candidum GN=BN980_GECA02s09063g PE=3 SV=1
A0A161HMS9_9ASCO47.93%7240.0Ncl1p OS=Sugiyamaella lignohabitans GN=NCL1 PE=3 SV=1
UniRef50_A0A0J9XJE049.02%7120.0Similar to Saccharomyces cerevisiae YBL024W NCL1 S-adenosyl-L-methionine-dependent tRNA: m5C-methyltransferase n=1 Tax=Geotrichum candidum TaxID=1173061 RepID=A0A0J9XJE0_GEOCN
A0A1E3PQQ8_9ASCO47.12%7110.0S-adenosyl-L-methionine-dependent methyltransferase OS=Nadsonia fulvescens var. elongata DSM 6958 GN=NADFUDRAFT_20543 PE=3 SV=1
Q6C6S1_YARLI46.41%7390.0YALI0E06765p OS=Yarrowia lipolytica (strain CLIB 122 / E 150) GN=YALI0_E06765g PE=3 SV=1
A0A1D8PSR2_CANAL45.48%7190.0tRNA (Cytosine-5-)-methyltransferase OS=Candida albicans (strain SC5314 / ATCC MYA-2876) GN=CAALFM_CR04300WA PE=3 SV=1
NCL1_YEAST43.00%7070.0Multisite-specific tRNA:(cytosine-C(5))-methyltransferase OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=NCL1 PE=1 SV=1
A0A1E4TKQ4_9ASCO46.77%6650.0Uncharacterized protein OS=Tortispora caseinolytica NRRL Y-17796 GN=CANCADRAFT_84943 PE=3 SV=1

Mitochondrial localization by mitoprotII

Probability of mitochondrial location: 0.3753
Predicted cleavage: 12

Protein family membership

Domains and repeats

  1. Domain
1 100 200 300 400 500 600 700 780

Detailed signature matches

  1. Family IPR023267
    RNA (C5-cytosine) methyltransferase
    1. PR02008 (RCMTFAMILY)
  2. Family IPR023270
    tRNA (C5-cytosine) methyltransferase, NCL1
    1. PR02011 (RCMTNCL1)
  3. Domain IPR029063
    S-adenosyl-L-methionine-dependent methyltransferase
    1. SSF53335 (S-adenosy...)
  4. Domain IPR001678
    SAM-dependent methyltransferase RsmB/NOP2-type
    1. PF01189 (Methyltr_R...)
    2. PS51686 (SAM_MT_RSM...)
  5. SITE IPR018314
    Bacterial Fmu (Sun)/eukaryotic nucleolar NOL1/Nop2p, conserved site
    1. PS01153 (NOL1_NOP2_SUN)
Unintegrated signatures no IPR
Unintegrated signatures
  1. G3DSA:3.40.50...
  2. mobidb-lite (disord...)

Protein sequence

>MCA_02073_1
MGRRGGGRTRSNGNWETATKENAKWEAYYKAQPNLVSEEEWDAFKQACQSALPLTFRVTSSTVASEEIKSLIQNDYVKHT
QDIVFQGEKISPPKPLPFYEPLGSAWQIDVGKQVIRRSKEFSRLQRFLVVETEAGNIYRQEAVSMIPPLFLDVKPHHAVI
DLCAAPGSKTAQLVEFLHQEGPSSIPTGFVLANDSDYKRAHMLIHQVKRLNSPNLIVTNHDAQMFPRMKTTSGEIVKFDR
VLCDVPCSGDGTMRKNVNVWTDWKIGNGNGLHNTQLNILLRGIQMLKPGGRLVYSTCSLNPVENEAVVAEALRRCGNTIG
LVDVSNMLPGLKRKPGVDTWKVQNKAGEWIQNPANTTTLTEDPANDDLPAEVTPAIQSKNNLPNSLFPPSEEEKKIFALE
RCVRVYPHQQNTGGFFITVFEKFDTKKRATEDKAEEEQEPVEAKKPKIDETVEAGEAKTAETTEATEAKTDEKNETPAST
TTTTTTPKETVPVNGTAGEEVFRFLSSEYPDIVKIWKFYNIESTFPGGRSTLLVRNTTGIPSRAIYYVAPSVKPLLELND
HKVKFVHAGLRIFSIQKSDSPCPWRVHAEAINIVAPHINKNPTEGENRYIVAKHLDILKHLIETEFPLLESLKEVDEDVY
KRVSELEEGTFILEIPRTLKNPEQPKEGEQQDKEESSKKVWDNILYPIWRGKRSVNLMLAKQDLAELVHRVFGQENIKAR
KTNNNGGGKPKSQQQQQPQKQSQEQSDKPQITEVKEDAAEETTLVVEEVKQEETETKQES

GO term prediction

Biological Process

None predicted.

Molecular Function

GO:0003723 RNA binding
GO:0008168 methyltransferase activity
GO:0016428 tRNA (cytosine-5-)-methyltransferase activity

Cellular Component

None predicted.