MIA_05532_1

Protein

Length

875 amino acids

Protein function

EGGNOG:	0PFTQ	GPH1	Phosphorylase is an important allosteric enzyme in carbohydrate metabolism. Enzymes from different sources differ in their regulatory mechanisms and in their natural substrates. However, all known phosphorylases share catalytic and structural properties (By similarity)
SGD closest match:	S000006364	GPH1	Glycogen phosphorylase
CGD closest match:	CAL0000189831	GPH1	Alpha-1,4 glucan phosphorylase

Protein alignments

	%id	Aln length
MCA_02090_1	86.121%	807	MCA_02090_1
A0A0J9X5Z1_GEOCN	79.886%	875	Alpha-1,4 glucan phosphorylase OS=Geotrichum candidum GN=BN980_GECA03s04388g PE=3 SV=1
A0A167DDG6_9ASCO	73.734%	849	Alpha-1,4 glucan phosphorylase OS=Sugiyamaella lignohabitans GN=GPH1 PE=3 SV=1
A0A060T314_BLAAD	72.459%	846	Alpha-1,4 glucan phosphorylase OS=Blastobotrys adeninivorans GN=GNLVRS02_ARAD1C29238g PE=3 SV=1
A0A1E3PQD2_9ASCO	72.620%	851	Alpha-1,4 glucan phosphorylase OS=Nadsonia fulvescens var. elongata DSM 6958 GN=NADFUDRAFT_40802 PE=3 SV=1
Q6C2Y0_YARLI	72.203%	849	Alpha-1,4 glucan phosphorylase OS=Yarrowia lipolytica (strain CLIB 122 / E 150) GN=YALI0_F04169g PE=3 SV=1
UniRef50_A0A1B8AG69	67.887%	847	Alpha-1,4 glucan phosphorylase n=15 Tax=leotiomyceta TaxID=716546 RepID=A0A1B8AG69_FUSPO
A0A1D8PQQ3_CANAL	62.514%	883	Alpha-1,4 glucan phosphorylase OS=Candida albicans (strain SC5314 / ATCC MYA-2876) GN=GPH1 PE=3 SV=1
PHSG_YEAST	61.635%	881	Glycogen phosphorylase OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=GPH1 PE=1 SV=4

Mitochondrial localization by mitoprotII

Probability of mitochondrial location: 0.0601

Protein family membership

Glycosyl transferase, family 35 (IPR000811)
- Glycogen/starch/alpha-glucan phosphorylase (IPR011833)

Domains and repeats

None predicted.

Detailed signature matches

IPR000811

Glycosyl transferase, family 35
1. PIRSF000460 (Glucan...)
  
  52 - 875
  
  PIRSF
  PIRSF000460 (Glucan_phosphorylase_GlgP)
2. PF00343 (Phosphorylase)
  
  155 - 869
  
  Pfam
  PF00343 (Phosphorylase)
IPR011833

Glycogen/starch/alpha-glucan phosphorylase
1. TIGR02093
  
  66 - 869
  
  TIGRFAMs
  TIGR02093 (TIGR02093)
2. cd04300 (GT1_Glycog...)
  
  70 - 869
  
  CDD
  cd04300 (GT1_Glycogen_Phosphorylase)
IPR035090

Phosphorylase pyridoxal-phosphate attachment site
1. PS00102 (PHOSPHORYLASE)
  
  716 - 728
  
  PROSITE patterns
  PS00102 (PHOSPHORYLASE)

no IPR

Unintegrated signatures

Coil

874 - 875

COILS
Coil (Coil)
G3DSA:3.40.50...

56 - 536

GENE3D
G3DSA:3.40.50.2000 (G3DSA:3.40.50.2000)

537 - 854

GENE3D
G3DSA:3.40.50.2000 (G3DSA:3.40.50.2000)
SSF53756 (UDP-Glyco...)

32 - 872

SUPERFAMILY
SSF53756 (UDP-Glycosyltransferase/glycogen phosphorylase)

Residue annotation

homodimer interfac...

70Q

homodimer interface
70Q, 72E, 74V, 75R, 77V, 78E, 79T, 102R, 103D, 104R, 235R, 329N, 332F
CDD
cd04300 (GT1_Glycogen_Phosphorylase)

72E

homodimer interface
70Q, 72E, 74V, 75R, 77V, 78E, 79T, 102R, 103D, 104R, 235R, 329N, 332F
CDD
cd04300 (GT1_Glycogen_Phosphorylase)

74V

homodimer interface
70Q, 72E, 74V, 75R, 77V, 78E, 79T, 102R, 103D, 104R, 235R, 329N, 332F
CDD
cd04300 (GT1_Glycogen_Phosphorylase)

75R

homodimer interface
70Q, 72E, 74V, 75R, 77V, 78E, 79T, 102R, 103D, 104R, 235R, 329N, 332F
CDD
cd04300 (GT1_Glycogen_Phosphorylase)

77V

homodimer interface
70Q, 72E, 74V, 75R, 77V, 78E, 79T, 102R, 103D, 104R, 235R, 329N, 332F
CDD
cd04300 (GT1_Glycogen_Phosphorylase)

78E

homodimer interface
70Q, 72E, 74V, 75R, 77V, 78E, 79T, 102R, 103D, 104R, 235R, 329N, 332F
CDD
cd04300 (GT1_Glycogen_Phosphorylase)

79T

homodimer interface
70Q, 72E, 74V, 75R, 77V, 78E, 79T, 102R, 103D, 104R, 235R, 329N, 332F
CDD
cd04300 (GT1_Glycogen_Phosphorylase)

102R

homodimer interface
70Q, 72E, 74V, 75R, 77V, 78E, 79T, 102R, 103D, 104R, 235R, 329N, 332F
CDD
cd04300 (GT1_Glycogen_Phosphorylase)

103D

homodimer interface
70Q, 72E, 74V, 75R, 77V, 78E, 79T, 102R, 103D, 104R, 235R, 329N, 332F
CDD
cd04300 (GT1_Glycogen_Phosphorylase)

104R

homodimer interface
70Q, 72E, 74V, 75R, 77V, 78E, 79T, 102R, 103D, 104R, 235R, 329N, 332F
CDD
cd04300 (GT1_Glycogen_Phosphorylase)

235R

homodimer interface
70Q, 72E, 74V, 75R, 77V, 78E, 79T, 102R, 103D, 104R, 235R, 329N, 332F
CDD
cd04300 (GT1_Glycogen_Phosphorylase)

329N

homodimer interface
70Q, 72E, 74V, 75R, 77V, 78E, 79T, 102R, 103D, 104R, 235R, 329N, 332F
CDD
cd04300 (GT1_Glycogen_Phosphorylase)

332F

homodimer interface
70Q, 72E, 74V, 75R, 77V, 78E, 79T, 102R, 103D, 104R, 235R, 329N, 332F
CDD
cd04300 (GT1_Glycogen_Phosphorylase)
active site pocket...

130E

active site pocket
130E, 132L, 176G, 177G, 178L, 327Y, 330D, 332F, 339R, 378D, 380H, 416H, 417T, 421E, 524N, 531W, 609K, 610R, 612H, 613E, 614Y, 615K, 652K, 657Y, 659M, 693N, 694V, 716E, 718S, 719G, 720T, 721S, 724K
CDD
cd04300 (GT1_Glycogen_Phosphorylase)

132L

active site pocket
130E, 132L, 176G, 177G, 178L, 327Y, 330D, 332F, 339R, 378D, 380H, 416H, 417T, 421E, 524N, 531W, 609K, 610R, 612H, 613E, 614Y, 615K, 652K, 657Y, 659M, 693N, 694V, 716E, 718S, 719G, 720T, 721S, 724K
CDD
cd04300 (GT1_Glycogen_Phosphorylase)

176G

active site pocket
130E, 132L, 176G, 177G, 178L, 327Y, 330D, 332F, 339R, 378D, 380H, 416H, 417T, 421E, 524N, 531W, 609K, 610R, 612H, 613E, 614Y, 615K, 652K, 657Y, 659M, 693N, 694V, 716E, 718S, 719G, 720T, 721S, 724K
CDD
cd04300 (GT1_Glycogen_Phosphorylase)

177G

active site pocket
130E, 132L, 176G, 177G, 178L, 327Y, 330D, 332F, 339R, 378D, 380H, 416H, 417T, 421E, 524N, 531W, 609K, 610R, 612H, 613E, 614Y, 615K, 652K, 657Y, 659M, 693N, 694V, 716E, 718S, 719G, 720T, 721S, 724K
CDD
cd04300 (GT1_Glycogen_Phosphorylase)

178L

active site pocket
130E, 132L, 176G, 177G, 178L, 327Y, 330D, 332F, 339R, 378D, 380H, 416H, 417T, 421E, 524N, 531W, 609K, 610R, 612H, 613E, 614Y, 615K, 652K, 657Y, 659M, 693N, 694V, 716E, 718S, 719G, 720T, 721S, 724K
CDD
cd04300 (GT1_Glycogen_Phosphorylase)

327Y

active site pocket
130E, 132L, 176G, 177G, 178L, 327Y, 330D, 332F, 339R, 378D, 380H, 416H, 417T, 421E, 524N, 531W, 609K, 610R, 612H, 613E, 614Y, 615K, 652K, 657Y, 659M, 693N, 694V, 716E, 718S, 719G, 720T, 721S, 724K
CDD
cd04300 (GT1_Glycogen_Phosphorylase)

330D

active site pocket
130E, 132L, 176G, 177G, 178L, 327Y, 330D, 332F, 339R, 378D, 380H, 416H, 417T, 421E, 524N, 531W, 609K, 610R, 612H, 613E, 614Y, 615K, 652K, 657Y, 659M, 693N, 694V, 716E, 718S, 719G, 720T, 721S, 724K
CDD
cd04300 (GT1_Glycogen_Phosphorylase)

332F

active site pocket
130E, 132L, 176G, 177G, 178L, 327Y, 330D, 332F, 339R, 378D, 380H, 416H, 417T, 421E, 524N, 531W, 609K, 610R, 612H, 613E, 614Y, 615K, 652K, 657Y, 659M, 693N, 694V, 716E, 718S, 719G, 720T, 721S, 724K
CDD
cd04300 (GT1_Glycogen_Phosphorylase)

339R

active site pocket
130E, 132L, 176G, 177G, 178L, 327Y, 330D, 332F, 339R, 378D, 380H, 416H, 417T, 421E, 524N, 531W, 609K, 610R, 612H, 613E, 614Y, 615K, 652K, 657Y, 659M, 693N, 694V, 716E, 718S, 719G, 720T, 721S, 724K
CDD
cd04300 (GT1_Glycogen_Phosphorylase)

378D

active site pocket
130E, 132L, 176G, 177G, 178L, 327Y, 330D, 332F, 339R, 378D, 380H, 416H, 417T, 421E, 524N, 531W, 609K, 610R, 612H, 613E, 614Y, 615K, 652K, 657Y, 659M, 693N, 694V, 716E, 718S, 719G, 720T, 721S, 724K
CDD
cd04300 (GT1_Glycogen_Phosphorylase)

380H

active site pocket
130E, 132L, 176G, 177G, 178L, 327Y, 330D, 332F, 339R, 378D, 380H, 416H, 417T, 421E, 524N, 531W, 609K, 610R, 612H, 613E, 614Y, 615K, 652K, 657Y, 659M, 693N, 694V, 716E, 718S, 719G, 720T, 721S, 724K
CDD
cd04300 (GT1_Glycogen_Phosphorylase)

416H

active site pocket
130E, 132L, 176G, 177G, 178L, 327Y, 330D, 332F, 339R, 378D, 380H, 416H, 417T, 421E, 524N, 531W, 609K, 610R, 612H, 613E, 614Y, 615K, 652K, 657Y, 659M, 693N, 694V, 716E, 718S, 719G, 720T, 721S, 724K
CDD
cd04300 (GT1_Glycogen_Phosphorylase)

417T

active site pocket
130E, 132L, 176G, 177G, 178L, 327Y, 330D, 332F, 339R, 378D, 380H, 416H, 417T, 421E, 524N, 531W, 609K, 610R, 612H, 613E, 614Y, 615K, 652K, 657Y, 659M, 693N, 694V, 716E, 718S, 719G, 720T, 721S, 724K
CDD
cd04300 (GT1_Glycogen_Phosphorylase)

421E

active site pocket
130E, 132L, 176G, 177G, 178L, 327Y, 330D, 332F, 339R, 378D, 380H, 416H, 417T, 421E, 524N, 531W, 609K, 610R, 612H, 613E, 614Y, 615K, 652K, 657Y, 659M, 693N, 694V, 716E, 718S, 719G, 720T, 721S, 724K
CDD
cd04300 (GT1_Glycogen_Phosphorylase)

524N

active site pocket
130E, 132L, 176G, 177G, 178L, 327Y, 330D, 332F, 339R, 378D, 380H, 416H, 417T, 421E, 524N, 531W, 609K, 610R, 612H, 613E, 614Y, 615K, 652K, 657Y, 659M, 693N, 694V, 716E, 718S, 719G, 720T, 721S, 724K
CDD
cd04300 (GT1_Glycogen_Phosphorylase)

531W

active site pocket
130E, 132L, 176G, 177G, 178L, 327Y, 330D, 332F, 339R, 378D, 380H, 416H, 417T, 421E, 524N, 531W, 609K, 610R, 612H, 613E, 614Y, 615K, 652K, 657Y, 659M, 693N, 694V, 716E, 718S, 719G, 720T, 721S, 724K
CDD
cd04300 (GT1_Glycogen_Phosphorylase)

609K

active site pocket
130E, 132L, 176G, 177G, 178L, 327Y, 330D, 332F, 339R, 378D, 380H, 416H, 417T, 421E, 524N, 531W, 609K, 610R, 612H, 613E, 614Y, 615K, 652K, 657Y, 659M, 693N, 694V, 716E, 718S, 719G, 720T, 721S, 724K
CDD
cd04300 (GT1_Glycogen_Phosphorylase)

610R

active site pocket
130E, 132L, 176G, 177G, 178L, 327Y, 330D, 332F, 339R, 378D, 380H, 416H, 417T, 421E, 524N, 531W, 609K, 610R, 612H, 613E, 614Y, 615K, 652K, 657Y, 659M, 693N, 694V, 716E, 718S, 719G, 720T, 721S, 724K
CDD
cd04300 (GT1_Glycogen_Phosphorylase)

612H

active site pocket
130E, 132L, 176G, 177G, 178L, 327Y, 330D, 332F, 339R, 378D, 380H, 416H, 417T, 421E, 524N, 531W, 609K, 610R, 612H, 613E, 614Y, 615K, 652K, 657Y, 659M, 693N, 694V, 716E, 718S, 719G, 720T, 721S, 724K
CDD
cd04300 (GT1_Glycogen_Phosphorylase)

613E

active site pocket
130E, 132L, 176G, 177G, 178L, 327Y, 330D, 332F, 339R, 378D, 380H, 416H, 417T, 421E, 524N, 531W, 609K, 610R, 612H, 613E, 614Y, 615K, 652K, 657Y, 659M, 693N, 694V, 716E, 718S, 719G, 720T, 721S, 724K
CDD
cd04300 (GT1_Glycogen_Phosphorylase)

614Y

active site pocket
130E, 132L, 176G, 177G, 178L, 327Y, 330D, 332F, 339R, 378D, 380H, 416H, 417T, 421E, 524N, 531W, 609K, 610R, 612H, 613E, 614Y, 615K, 652K, 657Y, 659M, 693N, 694V, 716E, 718S, 719G, 720T, 721S, 724K
CDD
cd04300 (GT1_Glycogen_Phosphorylase)

615K

active site pocket
130E, 132L, 176G, 177G, 178L, 327Y, 330D, 332F, 339R, 378D, 380H, 416H, 417T, 421E, 524N, 531W, 609K, 610R, 612H, 613E, 614Y, 615K, 652K, 657Y, 659M, 693N, 694V, 716E, 718S, 719G, 720T, 721S, 724K
CDD
cd04300 (GT1_Glycogen_Phosphorylase)

652K

active site pocket
130E, 132L, 176G, 177G, 178L, 327Y, 330D, 332F, 339R, 378D, 380H, 416H, 417T, 421E, 524N, 531W, 609K, 610R, 612H, 613E, 614Y, 615K, 652K, 657Y, 659M, 693N, 694V, 716E, 718S, 719G, 720T, 721S, 724K
CDD
cd04300 (GT1_Glycogen_Phosphorylase)

657Y

active site pocket
130E, 132L, 176G, 177G, 178L, 327Y, 330D, 332F, 339R, 378D, 380H, 416H, 417T, 421E, 524N, 531W, 609K, 610R, 612H, 613E, 614Y, 615K, 652K, 657Y, 659M, 693N, 694V, 716E, 718S, 719G, 720T, 721S, 724K
CDD
cd04300 (GT1_Glycogen_Phosphorylase)

659M

active site pocket
130E, 132L, 176G, 177G, 178L, 327Y, 330D, 332F, 339R, 378D, 380H, 416H, 417T, 421E, 524N, 531W, 609K, 610R, 612H, 613E, 614Y, 615K, 652K, 657Y, 659M, 693N, 694V, 716E, 718S, 719G, 720T, 721S, 724K
CDD
cd04300 (GT1_Glycogen_Phosphorylase)

693N

active site pocket
130E, 132L, 176G, 177G, 178L, 327Y, 330D, 332F, 339R, 378D, 380H, 416H, 417T, 421E, 524N, 531W, 609K, 610R, 612H, 613E, 614Y, 615K, 652K, 657Y, 659M, 693N, 694V, 716E, 718S, 719G, 720T, 721S, 724K
CDD
cd04300 (GT1_Glycogen_Phosphorylase)

694V

active site pocket
130E, 132L, 176G, 177G, 178L, 327Y, 330D, 332F, 339R, 378D, 380H, 416H, 417T, 421E, 524N, 531W, 609K, 610R, 612H, 613E, 614Y, 615K, 652K, 657Y, 659M, 693N, 694V, 716E, 718S, 719G, 720T, 721S, 724K
CDD
cd04300 (GT1_Glycogen_Phosphorylase)

716E

active site pocket
130E, 132L, 176G, 177G, 178L, 327Y, 330D, 332F, 339R, 378D, 380H, 416H, 417T, 421E, 524N, 531W, 609K, 610R, 612H, 613E, 614Y, 615K, 652K, 657Y, 659M, 693N, 694V, 716E, 718S, 719G, 720T, 721S, 724K
CDD
cd04300 (GT1_Glycogen_Phosphorylase)

718S

active site pocket
130E, 132L, 176G, 177G, 178L, 327Y, 330D, 332F, 339R, 378D, 380H, 416H, 417T, 421E, 524N, 531W, 609K, 610R, 612H, 613E, 614Y, 615K, 652K, 657Y, 659M, 693N, 694V, 716E, 718S, 719G, 720T, 721S, 724K
CDD
cd04300 (GT1_Glycogen_Phosphorylase)

719G

active site pocket
130E, 132L, 176G, 177G, 178L, 327Y, 330D, 332F, 339R, 378D, 380H, 416H, 417T, 421E, 524N, 531W, 609K, 610R, 612H, 613E, 614Y, 615K, 652K, 657Y, 659M, 693N, 694V, 716E, 718S, 719G, 720T, 721S, 724K
CDD
cd04300 (GT1_Glycogen_Phosphorylase)

720T

active site pocket
130E, 132L, 176G, 177G, 178L, 327Y, 330D, 332F, 339R, 378D, 380H, 416H, 417T, 421E, 524N, 531W, 609K, 610R, 612H, 613E, 614Y, 615K, 652K, 657Y, 659M, 693N, 694V, 716E, 718S, 719G, 720T, 721S, 724K
CDD
cd04300 (GT1_Glycogen_Phosphorylase)

721S

active site pocket
130E, 132L, 176G, 177G, 178L, 327Y, 330D, 332F, 339R, 378D, 380H, 416H, 417T, 421E, 524N, 531W, 609K, 610R, 612H, 613E, 614Y, 615K, 652K, 657Y, 659M, 693N, 694V, 716E, 718S, 719G, 720T, 721S, 724K
CDD
cd04300 (GT1_Glycogen_Phosphorylase)

724K

active site pocket
130E, 132L, 176G, 177G, 178L, 327Y, 330D, 332F, 339R, 378D, 380H, 416H, 417T, 421E, 524N, 531W, 609K, 610R, 612H, 613E, 614Y, 615K, 652K, 657Y, 659M, 693N, 694V, 716E, 718S, 719G, 720T, 721S, 724K
CDD
cd04300 (GT1_Glycogen_Phosphorylase)

Protein sequence

>MIA_05532_1
MSLFKNYGGVSPAAAVDEEEEVFVPRRHKRTFTGFTPRELKAIDSTIPIGCRNTWKKFAIGEFSSKDQFQAEFVRHVETT
LARSLYNCDELAAYQAASQAIRDRLIVAWNKTQQKHTLVDPKRIYYLSLEFLMGRAMDNAMLNLEVKKVTGDGVSEFGFR
LEDLIEQEKDAALGNGGLGRLAACFIDSLSTLNYPGWGYGLRYQYGIFKQKIVDGYQVEQPDYWLTFNNPWEIPRAEIAI
DVMFYGYVRKTTDDEGSQKKVWEGGQIVQAVAYDFPVPGYGTENVNNLRLWSSKPNRAFDFAKFNEGAYNQALRDQQEAE
TISAVLYPNDNFYSGKELRLKQQYFWVAASLSDIVRRFKKTHRPWNEFSDQIAIQLNDTHPTLAIPELQRILVDREGLPW
DEAWGIVKETFGYTNHTVLPEALEKWPVNLIGNLLPRHLEIIYDINLKFLQEVSRKFPGDSDLVRDVSLIEEGGNRQVRM
AYLAIVGSHKVNGVAELHSELIRTTIFKNFVKIYGPDKFTNVTNGVTPRRWLHQANPELSALIAEKLGGYKYLNNLGQLK
ELLKYKDDPAFQEKWTATKRANKARLAALVKSQTGVELNLDALFDIQVKRIHEYKRQQMNIFQVIHRYLRLKLMTPEELE
KIPPRVSIFGGKSAPGYYMAKNIIKLINSVGQVVNNDESIKDKLKVIFIEDYNVSKAEIIIPASDISEHISTAGTEASGT
SNMKFVLNGGLIIGTVDGANIEITREITEDNIFLFGHLAEEIEDLRLAHRTGATTLDPQLAKVFKAIESGTFGDATIYND
LINSIAGYGSDHYLVSDDFSSYLDAQDKVDEAFLDKKKWVVKSIEAVANMGFFSSDRAIDEYAETIWNIEPVPLK

GO term prediction

Biological Process

GO:0005975 carbohydrate metabolic process

Molecular Function

GO:0004645 phosphorylase activity
GO:0008184 glycogen phosphorylase activity
GO:0030170 pyridoxal phosphate binding

Cellular Component