MCA_02090_1

Protein

Length

811 amino acids

Gene name: GPH1

Description: Glycogen phosphorylase

Browser: contigB:207072-209508+

RNA-seq: read pairs 35163, FPKM 535.1, percentile rank 94.5% (100% = highest expression)

Protein function

Annotation:		GPH1	Glycogen phosphorylase
KEGG:	K00688	PYG	glycogen phosphorylase [EC:2.4.1.1]
EGGNOG:	0PFTQ	GPH1	Phosphorylase is an important allosteric enzyme in carbohydrate metabolism. Enzymes from different sources differ in their regulatory mechanisms and in their natural substrates. However, all known phosphorylases share catalytic and structural properties (By similarity)
SGD closest match:	S000006364	GPH1	Glycogen phosphorylase
CGD closest match:	CAL0000189831	GPH1	Alpha-1,4 glucan phosphorylase

Protein alignments

	%id	Aln length
MIA_05532_1	86.12%	807	MIA_05532_1
A0A0J9X5Z1_GEOCN	83.04%	808	Alpha-1,4 glucan phosphorylase OS=Geotrichum candidum GN=BN980_GECA03s04388g PE=3 SV=1
A0A167DDG6_9ASCO	74.60%	807	Alpha-1,4 glucan phosphorylase OS=Sugiyamaella lignohabitans GN=GPH1 PE=3 SV=1
A0A060T314_BLAAD	73.33%	806	Alpha-1,4 glucan phosphorylase OS=Blastobotrys adeninivorans GN=GNLVRS02_ARAD1C29238g PE=3 SV=1
A0A1E3PQD2_9ASCO	73.18%	809	Alpha-1,4 glucan phosphorylase OS=Nadsonia fulvescens var. elongata DSM 6958 GN=NADFUDRAFT_40802 PE=3 SV=1
Q6C2Y0_YARLI	73.89%	808	Alpha-1,4 glucan phosphorylase OS=Yarrowia lipolytica (strain CLIB 122 / E 150) GN=YALI0_F04169g PE=3 SV=1
UniRef50_A0A1B8AG69	71.68%	805	Alpha-1,4 glucan phosphorylase n=15 Tax=leotiomyceta TaxID=716546 RepID=A0A1B8AG69_FUSPO
A0A1D8PQQ3_CANAL	66.67%	825	Alpha-1,4 glucan phosphorylase OS=Candida albicans (strain SC5314 / ATCC MYA-2876) GN=GPH1 PE=3 SV=1
PHSG_YEAST	65.12%	840	Glycogen phosphorylase OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=GPH1 PE=1 SV=4

Mitochondrial localization by mitoprotII

Probability of mitochondrial location: 0.3808

Protein family membership

Glycosyl transferase, family 35 (IPR000811)
- Glycogen/starch/alpha-glucan phosphorylase (IPR011833)

Domains and repeats

None predicted.

Detailed signature matches

IPR000811

Glycosyl transferase, family 35
1. PIRSF000460 (Glucan...)
  
  1 - 811
  
  PIRSF
  PIRSF000460 (Glucan_phosphorylase_GlgP)
2. PF00343 (Phosphorylase)
  
  92 - 806
  
  Pfam
  PF00343 (Phosphorylase)
IPR011833

Glycogen/starch/alpha-glucan phosphorylase
1. TIGR02093
  
  4 - 806
  
  TIGRFAMs
  TIGR02093 (TIGR02093)
2. cd04300 (GT1_Glycog...)
  
  7 - 806
  
  CDD
  cd04300 (GT1_Glycogen_Phosphorylase)
IPR035090

Phosphorylase pyridoxal-phosphate attachment site
1. PS00102 (PHOSPHORYLASE)
  
  653 - 665
  
  PROSITE patterns
  PS00102 (PHOSPHORYLASE)

no IPR

Unintegrated signatures

G3DSA:3.40.50...

474 - 791

GENE3D
G3DSA:3.40.50.2000 (G3DSA:3.40.50.2000)

2 - 473

GENE3D
G3DSA:3.40.50.2000 (G3DSA:3.40.50.2000)
SSF53756 (UDP-Glyco...)

4 - 809

SUPERFAMILY
SSF53756 (UDP-Glycosyltransferase/glycogen phosphorylase)

Residue annotation

homodimer interfac...

7Q

homodimer interface
7Q, 9E, 11V, 12R, 14V, 15E, 16T, 39R, 40D, 41R, 172R, 266N, 269F
CDD
cd04300 (GT1_Glycogen_Phosphorylase)

9E

homodimer interface
7Q, 9E, 11V, 12R, 14V, 15E, 16T, 39R, 40D, 41R, 172R, 266N, 269F
CDD
cd04300 (GT1_Glycogen_Phosphorylase)

11V

homodimer interface
7Q, 9E, 11V, 12R, 14V, 15E, 16T, 39R, 40D, 41R, 172R, 266N, 269F
CDD
cd04300 (GT1_Glycogen_Phosphorylase)

12R

homodimer interface
7Q, 9E, 11V, 12R, 14V, 15E, 16T, 39R, 40D, 41R, 172R, 266N, 269F
CDD
cd04300 (GT1_Glycogen_Phosphorylase)

14V

homodimer interface
7Q, 9E, 11V, 12R, 14V, 15E, 16T, 39R, 40D, 41R, 172R, 266N, 269F
CDD
cd04300 (GT1_Glycogen_Phosphorylase)

15E

homodimer interface
7Q, 9E, 11V, 12R, 14V, 15E, 16T, 39R, 40D, 41R, 172R, 266N, 269F
CDD
cd04300 (GT1_Glycogen_Phosphorylase)

16T

homodimer interface
7Q, 9E, 11V, 12R, 14V, 15E, 16T, 39R, 40D, 41R, 172R, 266N, 269F
CDD
cd04300 (GT1_Glycogen_Phosphorylase)

39R

homodimer interface
7Q, 9E, 11V, 12R, 14V, 15E, 16T, 39R, 40D, 41R, 172R, 266N, 269F
CDD
cd04300 (GT1_Glycogen_Phosphorylase)

40D

homodimer interface
7Q, 9E, 11V, 12R, 14V, 15E, 16T, 39R, 40D, 41R, 172R, 266N, 269F
CDD
cd04300 (GT1_Glycogen_Phosphorylase)

41R

homodimer interface
7Q, 9E, 11V, 12R, 14V, 15E, 16T, 39R, 40D, 41R, 172R, 266N, 269F
CDD
cd04300 (GT1_Glycogen_Phosphorylase)

172R

homodimer interface
7Q, 9E, 11V, 12R, 14V, 15E, 16T, 39R, 40D, 41R, 172R, 266N, 269F
CDD
cd04300 (GT1_Glycogen_Phosphorylase)

266N

homodimer interface
7Q, 9E, 11V, 12R, 14V, 15E, 16T, 39R, 40D, 41R, 172R, 266N, 269F
CDD
cd04300 (GT1_Glycogen_Phosphorylase)

269F

homodimer interface
7Q, 9E, 11V, 12R, 14V, 15E, 16T, 39R, 40D, 41R, 172R, 266N, 269F
CDD
cd04300 (GT1_Glycogen_Phosphorylase)
active site pocket...

67E

active site pocket
67E, 69L, 113G, 114G, 115L, 264Y, 267D, 269F, 276R, 315D, 317H, 353H, 354T, 358E, 461N, 468W, 546K, 547R, 549H, 550E, 551Y, 552K, 589K, 594Y, 596M, 630N, 631V, 653E, 655S, 656G, 657T, 658S, 661K
CDD
cd04300 (GT1_Glycogen_Phosphorylase)

69L

active site pocket
67E, 69L, 113G, 114G, 115L, 264Y, 267D, 269F, 276R, 315D, 317H, 353H, 354T, 358E, 461N, 468W, 546K, 547R, 549H, 550E, 551Y, 552K, 589K, 594Y, 596M, 630N, 631V, 653E, 655S, 656G, 657T, 658S, 661K
CDD
cd04300 (GT1_Glycogen_Phosphorylase)

113G

active site pocket
67E, 69L, 113G, 114G, 115L, 264Y, 267D, 269F, 276R, 315D, 317H, 353H, 354T, 358E, 461N, 468W, 546K, 547R, 549H, 550E, 551Y, 552K, 589K, 594Y, 596M, 630N, 631V, 653E, 655S, 656G, 657T, 658S, 661K
CDD
cd04300 (GT1_Glycogen_Phosphorylase)

114G

active site pocket
67E, 69L, 113G, 114G, 115L, 264Y, 267D, 269F, 276R, 315D, 317H, 353H, 354T, 358E, 461N, 468W, 546K, 547R, 549H, 550E, 551Y, 552K, 589K, 594Y, 596M, 630N, 631V, 653E, 655S, 656G, 657T, 658S, 661K
CDD
cd04300 (GT1_Glycogen_Phosphorylase)

115L

active site pocket
67E, 69L, 113G, 114G, 115L, 264Y, 267D, 269F, 276R, 315D, 317H, 353H, 354T, 358E, 461N, 468W, 546K, 547R, 549H, 550E, 551Y, 552K, 589K, 594Y, 596M, 630N, 631V, 653E, 655S, 656G, 657T, 658S, 661K
CDD
cd04300 (GT1_Glycogen_Phosphorylase)

264Y

active site pocket
67E, 69L, 113G, 114G, 115L, 264Y, 267D, 269F, 276R, 315D, 317H, 353H, 354T, 358E, 461N, 468W, 546K, 547R, 549H, 550E, 551Y, 552K, 589K, 594Y, 596M, 630N, 631V, 653E, 655S, 656G, 657T, 658S, 661K
CDD
cd04300 (GT1_Glycogen_Phosphorylase)

267D

active site pocket
67E, 69L, 113G, 114G, 115L, 264Y, 267D, 269F, 276R, 315D, 317H, 353H, 354T, 358E, 461N, 468W, 546K, 547R, 549H, 550E, 551Y, 552K, 589K, 594Y, 596M, 630N, 631V, 653E, 655S, 656G, 657T, 658S, 661K
CDD
cd04300 (GT1_Glycogen_Phosphorylase)

269F

active site pocket
67E, 69L, 113G, 114G, 115L, 264Y, 267D, 269F, 276R, 315D, 317H, 353H, 354T, 358E, 461N, 468W, 546K, 547R, 549H, 550E, 551Y, 552K, 589K, 594Y, 596M, 630N, 631V, 653E, 655S, 656G, 657T, 658S, 661K
CDD
cd04300 (GT1_Glycogen_Phosphorylase)

276R

active site pocket
67E, 69L, 113G, 114G, 115L, 264Y, 267D, 269F, 276R, 315D, 317H, 353H, 354T, 358E, 461N, 468W, 546K, 547R, 549H, 550E, 551Y, 552K, 589K, 594Y, 596M, 630N, 631V, 653E, 655S, 656G, 657T, 658S, 661K
CDD
cd04300 (GT1_Glycogen_Phosphorylase)

315D

active site pocket
67E, 69L, 113G, 114G, 115L, 264Y, 267D, 269F, 276R, 315D, 317H, 353H, 354T, 358E, 461N, 468W, 546K, 547R, 549H, 550E, 551Y, 552K, 589K, 594Y, 596M, 630N, 631V, 653E, 655S, 656G, 657T, 658S, 661K
CDD
cd04300 (GT1_Glycogen_Phosphorylase)

317H

active site pocket
67E, 69L, 113G, 114G, 115L, 264Y, 267D, 269F, 276R, 315D, 317H, 353H, 354T, 358E, 461N, 468W, 546K, 547R, 549H, 550E, 551Y, 552K, 589K, 594Y, 596M, 630N, 631V, 653E, 655S, 656G, 657T, 658S, 661K
CDD
cd04300 (GT1_Glycogen_Phosphorylase)

353H

active site pocket
67E, 69L, 113G, 114G, 115L, 264Y, 267D, 269F, 276R, 315D, 317H, 353H, 354T, 358E, 461N, 468W, 546K, 547R, 549H, 550E, 551Y, 552K, 589K, 594Y, 596M, 630N, 631V, 653E, 655S, 656G, 657T, 658S, 661K
CDD
cd04300 (GT1_Glycogen_Phosphorylase)

354T

active site pocket
67E, 69L, 113G, 114G, 115L, 264Y, 267D, 269F, 276R, 315D, 317H, 353H, 354T, 358E, 461N, 468W, 546K, 547R, 549H, 550E, 551Y, 552K, 589K, 594Y, 596M, 630N, 631V, 653E, 655S, 656G, 657T, 658S, 661K
CDD
cd04300 (GT1_Glycogen_Phosphorylase)

358E

active site pocket
67E, 69L, 113G, 114G, 115L, 264Y, 267D, 269F, 276R, 315D, 317H, 353H, 354T, 358E, 461N, 468W, 546K, 547R, 549H, 550E, 551Y, 552K, 589K, 594Y, 596M, 630N, 631V, 653E, 655S, 656G, 657T, 658S, 661K
CDD
cd04300 (GT1_Glycogen_Phosphorylase)

461N

active site pocket
67E, 69L, 113G, 114G, 115L, 264Y, 267D, 269F, 276R, 315D, 317H, 353H, 354T, 358E, 461N, 468W, 546K, 547R, 549H, 550E, 551Y, 552K, 589K, 594Y, 596M, 630N, 631V, 653E, 655S, 656G, 657T, 658S, 661K
CDD
cd04300 (GT1_Glycogen_Phosphorylase)

468W

active site pocket
67E, 69L, 113G, 114G, 115L, 264Y, 267D, 269F, 276R, 315D, 317H, 353H, 354T, 358E, 461N, 468W, 546K, 547R, 549H, 550E, 551Y, 552K, 589K, 594Y, 596M, 630N, 631V, 653E, 655S, 656G, 657T, 658S, 661K
CDD
cd04300 (GT1_Glycogen_Phosphorylase)

546K

active site pocket
67E, 69L, 113G, 114G, 115L, 264Y, 267D, 269F, 276R, 315D, 317H, 353H, 354T, 358E, 461N, 468W, 546K, 547R, 549H, 550E, 551Y, 552K, 589K, 594Y, 596M, 630N, 631V, 653E, 655S, 656G, 657T, 658S, 661K
CDD
cd04300 (GT1_Glycogen_Phosphorylase)

547R

active site pocket
67E, 69L, 113G, 114G, 115L, 264Y, 267D, 269F, 276R, 315D, 317H, 353H, 354T, 358E, 461N, 468W, 546K, 547R, 549H, 550E, 551Y, 552K, 589K, 594Y, 596M, 630N, 631V, 653E, 655S, 656G, 657T, 658S, 661K
CDD
cd04300 (GT1_Glycogen_Phosphorylase)

549H

active site pocket
67E, 69L, 113G, 114G, 115L, 264Y, 267D, 269F, 276R, 315D, 317H, 353H, 354T, 358E, 461N, 468W, 546K, 547R, 549H, 550E, 551Y, 552K, 589K, 594Y, 596M, 630N, 631V, 653E, 655S, 656G, 657T, 658S, 661K
CDD
cd04300 (GT1_Glycogen_Phosphorylase)

550E

active site pocket
67E, 69L, 113G, 114G, 115L, 264Y, 267D, 269F, 276R, 315D, 317H, 353H, 354T, 358E, 461N, 468W, 546K, 547R, 549H, 550E, 551Y, 552K, 589K, 594Y, 596M, 630N, 631V, 653E, 655S, 656G, 657T, 658S, 661K
CDD
cd04300 (GT1_Glycogen_Phosphorylase)

551Y

active site pocket
67E, 69L, 113G, 114G, 115L, 264Y, 267D, 269F, 276R, 315D, 317H, 353H, 354T, 358E, 461N, 468W, 546K, 547R, 549H, 550E, 551Y, 552K, 589K, 594Y, 596M, 630N, 631V, 653E, 655S, 656G, 657T, 658S, 661K
CDD
cd04300 (GT1_Glycogen_Phosphorylase)

552K

active site pocket
67E, 69L, 113G, 114G, 115L, 264Y, 267D, 269F, 276R, 315D, 317H, 353H, 354T, 358E, 461N, 468W, 546K, 547R, 549H, 550E, 551Y, 552K, 589K, 594Y, 596M, 630N, 631V, 653E, 655S, 656G, 657T, 658S, 661K
CDD
cd04300 (GT1_Glycogen_Phosphorylase)

589K

active site pocket
67E, 69L, 113G, 114G, 115L, 264Y, 267D, 269F, 276R, 315D, 317H, 353H, 354T, 358E, 461N, 468W, 546K, 547R, 549H, 550E, 551Y, 552K, 589K, 594Y, 596M, 630N, 631V, 653E, 655S, 656G, 657T, 658S, 661K
CDD
cd04300 (GT1_Glycogen_Phosphorylase)

594Y

active site pocket
67E, 69L, 113G, 114G, 115L, 264Y, 267D, 269F, 276R, 315D, 317H, 353H, 354T, 358E, 461N, 468W, 546K, 547R, 549H, 550E, 551Y, 552K, 589K, 594Y, 596M, 630N, 631V, 653E, 655S, 656G, 657T, 658S, 661K
CDD
cd04300 (GT1_Glycogen_Phosphorylase)

596M

active site pocket
67E, 69L, 113G, 114G, 115L, 264Y, 267D, 269F, 276R, 315D, 317H, 353H, 354T, 358E, 461N, 468W, 546K, 547R, 549H, 550E, 551Y, 552K, 589K, 594Y, 596M, 630N, 631V, 653E, 655S, 656G, 657T, 658S, 661K
CDD
cd04300 (GT1_Glycogen_Phosphorylase)

630N

active site pocket
67E, 69L, 113G, 114G, 115L, 264Y, 267D, 269F, 276R, 315D, 317H, 353H, 354T, 358E, 461N, 468W, 546K, 547R, 549H, 550E, 551Y, 552K, 589K, 594Y, 596M, 630N, 631V, 653E, 655S, 656G, 657T, 658S, 661K
CDD
cd04300 (GT1_Glycogen_Phosphorylase)

631V

active site pocket
67E, 69L, 113G, 114G, 115L, 264Y, 267D, 269F, 276R, 315D, 317H, 353H, 354T, 358E, 461N, 468W, 546K, 547R, 549H, 550E, 551Y, 552K, 589K, 594Y, 596M, 630N, 631V, 653E, 655S, 656G, 657T, 658S, 661K
CDD
cd04300 (GT1_Glycogen_Phosphorylase)

653E

active site pocket
67E, 69L, 113G, 114G, 115L, 264Y, 267D, 269F, 276R, 315D, 317H, 353H, 354T, 358E, 461N, 468W, 546K, 547R, 549H, 550E, 551Y, 552K, 589K, 594Y, 596M, 630N, 631V, 653E, 655S, 656G, 657T, 658S, 661K
CDD
cd04300 (GT1_Glycogen_Phosphorylase)

655S

active site pocket
67E, 69L, 113G, 114G, 115L, 264Y, 267D, 269F, 276R, 315D, 317H, 353H, 354T, 358E, 461N, 468W, 546K, 547R, 549H, 550E, 551Y, 552K, 589K, 594Y, 596M, 630N, 631V, 653E, 655S, 656G, 657T, 658S, 661K
CDD
cd04300 (GT1_Glycogen_Phosphorylase)

656G

active site pocket
67E, 69L, 113G, 114G, 115L, 264Y, 267D, 269F, 276R, 315D, 317H, 353H, 354T, 358E, 461N, 468W, 546K, 547R, 549H, 550E, 551Y, 552K, 589K, 594Y, 596M, 630N, 631V, 653E, 655S, 656G, 657T, 658S, 661K
CDD
cd04300 (GT1_Glycogen_Phosphorylase)

657T

active site pocket
67E, 69L, 113G, 114G, 115L, 264Y, 267D, 269F, 276R, 315D, 317H, 353H, 354T, 358E, 461N, 468W, 546K, 547R, 549H, 550E, 551Y, 552K, 589K, 594Y, 596M, 630N, 631V, 653E, 655S, 656G, 657T, 658S, 661K
CDD
cd04300 (GT1_Glycogen_Phosphorylase)

658S

active site pocket
67E, 69L, 113G, 114G, 115L, 264Y, 267D, 269F, 276R, 315D, 317H, 353H, 354T, 358E, 461N, 468W, 546K, 547R, 549H, 550E, 551Y, 552K, 589K, 594Y, 596M, 630N, 631V, 653E, 655S, 656G, 657T, 658S, 661K
CDD
cd04300 (GT1_Glycogen_Phosphorylase)

661K

active site pocket
67E, 69L, 113G, 114G, 115L, 264Y, 267D, 269F, 276R, 315D, 317H, 353H, 354T, 358E, 461N, 468W, 546K, 547R, 549H, 550E, 551Y, 552K, 589K, 594Y, 596M, 630N, 631V, 653E, 655S, 656G, 657T, 658S, 661K
CDD
cd04300 (GT1_Glycogen_Phosphorylase)

Protein sequence

>MCA_02090_1
MIHQKFQAEFVRHVETTLARSLFNCDEFAAYQAASQAIRDRLIVAWNKTQQKHTSADPKRIYYLSLEFLMGRAMDNAMLN
LDVKQVTREGVSEFGFRLEDLIEQEKDAALGNGGLGRLAACFIDSLSTLNYPGWGYGLRYQYGIFKQKIVDGYQVELPDY
WLTFNNPWEIPRSEIAIDITFYGYVRRVTDDDGKQKKVWEGGELVQAVAYDFPVPGYGTENVNNLRLWSSKPNREFDFAK
FNEGAYDQAVQDQQRAETISAVLYPNDNFYSGKELRLKQQYFWVAASLSDIVRRFKKSQRPWREFSDQIAIQLNDTHPTL
AIPELQRILVDREGLDWDEAWGIVTKTFGYTNHTVLPEALEKWSVDLVGNLLPRHLEIIYDINFNFLQEVSKKFPNDPDL
IRRVSIIEEGTNRQVRMAFLAIIGSHKVNGVAELHSELIKTTIFKDFVKIYGEDKFTNVTNGVTPRRWLRQANPTLSSLI
ADKLGGYDYINKLGDLKQLLKFKDDPKFQDQWAATKRANKAQLAALVKSQTGVELNLDALFDIQVKRIHEYKRQQMNIFS
VIHRYLKIKAMTPEERKKVVPRVSIFGGKSAPGYYMAKVIIKLINAVGSVVNNDEDIGDLLKVIFVADYNVSKAEIIIPA
SDISEHISTAGTEASGTSNMKFVLNGGLIIGTVDGANIEITREIGESNIFLFGHLSESVEDLRNSHQFGHESLNPDLAKV
FESIQSGTFGDPNIYSGLIDSIAGHGSDYYLVSDDFASYLEAHDKVDAAFVDKKSWIVKSITAVANMGFFSSDRAIDEYA
ENIWNVEPVPL

GO term prediction

Biological Process

GO:0005975 carbohydrate metabolic process

Molecular Function

GO:0004645 phosphorylase activity
GO:0008184 glycogen phosphorylase activity
GO:0030170 pyridoxal phosphate binding

Cellular Component