MIA_05440_1

Protein

Length

2,077 amino acids

Protein function

EGGNOG:	0Q0WA	FAS1	fatty acid synthase
SGD closest match:	S000001665	FAS1	Fatty acid synthase subunit beta
CGD closest match:	CAL0000183162	FAS1	Tetrafunctional fatty acid synthase subunit

Protein alignments

	%id	Aln length
MCA_00734_1	82.12%	2081	MCA_00734_1
A0A0J9XBS8_GEOCN	76.85%	2078	Similar to Saccharomyces cerevisiae YKL182W FAS1 Beta subunit of fatty acid synthetase OS=Geotrichum candidum GN=BN980_GECA08s03893g PE=4 SV=1
A0A060T4D9_BLAAD	73.26%	2079	ARAD1A16258p OS=Blastobotrys adeninivorans GN=GNLVRS02_ARAD1A16258g PE=4 SV=1
A0A167D8A8_9ASCO	72.62%	2082	Tetrafunctional fatty acid synthase subunit FAS1 OS=Sugiyamaella lignohabitans GN=FAS1 PE=4 SV=1
A0A1E3PLT4_9ASCO	68.10%	2088	S-acyl fatty acid synthase thioesterase OS=Nadsonia fulvescens var. elongata DSM 6958 GN=FAS1 PE=4 SV=1
FAS1_YARLI	66.86%	2088	Fatty acid synthase subunit beta OS=Yarrowia lipolytica (strain CLIB 122 / E 150) GN=FAS1 PE=3 SV=2
Q5A4W7_CANAL	62.29%	2066	Tetrafunctional fatty acid synthase subunit OS=Candida albicans (strain SC5314 / ATCC MYA-2876) GN=FAS1 PE=4 SV=1
A0A1E4TI19_9ASCO	61.01%	2085	Uncharacterized protein OS=Tortispora caseinolytica NRRL Y-17796 GN=CANCADRAFT_32053 PE=4 SV=1
UniRef50_K0KL43	61.36%	2078	Fatty acid synthase n=39 Tax=saccharomyceta TaxID=716545 RepID=K0KL43_WICCF
FAS1_YEAST	57.67%	2072	Fatty acid synthase subunit beta OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=FAS1 PE=1 SV=2

Mitochondrial localization by mitoprotII

Probability of mitochondrial location: 0.0380

Protein family membership

Fatty acid synthase, beta subunit, fungi (IPR016452)

Domains and repeats

Domain
166 - 327
Acyl transferase/acyl hydrolase/lysophospholipase (IPR016035)
435 - 531
Acyl transferase/acyl hydrolase/lysophospholipase (IPR016035)
574 - 1,115
Aldolase-type TIM barrel (IPR013785)
1,305 - 1,436
HotDog domain (IPR029069)
1,545 - 1,682
HotDog domain (IPR029069)
MaoC-like domain (IPR002539)
1,689 - 2,062
Acyl transferase/acyl hydrolase/lysophospholipase (IPR016035)
Acyl transferase (IPR014043)
Polyketide synthase, acyl transferase domain (IPR020801)
Domain
169 - 397
Starter unit:ACP transacylase (IPR032088)
1,030 - 1,086
Domain of unknown function DUF1729 (IPR013565)
Domain
591 - 611
Fatty acid synthase (IPR003965)
617 - 635
Fatty acid synthase (IPR003965)
716 - 734
Fatty acid synthase (IPR003965)
736 - 755
Fatty acid synthase (IPR003965)
924 - 942
Fatty acid synthase (IPR003965)
1,024 - 1,048
Fatty acid synthase (IPR003965)
1,571 - 1,593
Fatty acid synthase (IPR003965)
1,593 - 1,614
Fatty acid synthase (IPR003965)
1,679 - 1,698
Fatty acid synthase (IPR003965)
1,700 - 1,723
Fatty acid synthase (IPR003965)
1,822 - 1,841
Fatty acid synthase (IPR003965)
1,858 - 1,879
Fatty acid synthase (IPR003965)

1 200 400 600 800 1000 1200 1400 1600 1800 2077

Detailed signature matches

IPR016452

Fatty acid synthase, beta subunit, fungi
1. PIRSF005562 (FAS_ye...)
  
  11 - 2,076
  
  PIRSF
  PIRSF005562 (FAS_yeast_beta)
IPR016035

Acyl transferase/acyl hydrolase/lysophospholipase
1. SSF52151 (FabD/lyso...)
  
  166 - 327
  
  SUPERFAMILY
  SSF52151 (FabD/lysophospholipase-like)
  
  435 - 531
  
  SUPERFAMILY
  SSF52151 (FabD/lysophospholipase-like)
  
  2,000 - 2,045
  
  SUPERFAMILY
  SSF52151 (FabD/lysophospholipase-like)
  
  1,795 - 1,871
  
  SUPERFAMILY
  SSF52151 (FabD/lysophospholipase-like)
  
  1,689 - 1,740
  
  SUPERFAMILY
  SSF52151 (FabD/lysophospholipase-like)
IPR032088

Starter unit:ACP transacylase
1. PF16073 (SAT)
  
  169 - 397
  
  Pfam
  PF16073 (SAT)
IPR013785

Aldolase-type TIM barrel
1. G3DSA:3.20.20.70
  
  574 - 1,115
  
  GENE3D
  G3DSA:3.20.20.70 (G3DSA:3.20.20.70)
IPR003965

Fatty acid synthase
1. PR01483 (FASYNTHASE)
  
  591 - 611
  
  PRINTS
  PR01483 (FASYNTHASE)
  
  716 - 734
  
  PRINTS
  PR01483 (FASYNTHASE)
  
  1,858 - 1,879
  
  PRINTS
  PR01483 (FASYNTHASE)
  
  924 - 942
  
  PRINTS
  PR01483 (FASYNTHASE)
  
  617 - 635
  
  PRINTS
  PR01483 (FASYNTHASE)
  
  1,593 - 1,614
  
  PRINTS
  PR01483 (FASYNTHASE)
  
  1,679 - 1,698
  
  PRINTS
  PR01483 (FASYNTHASE)
  
  736 - 755
  
  PRINTS
  PR01483 (FASYNTHASE)
  
  1,024 - 1,048
  
  PRINTS
  PR01483 (FASYNTHASE)
  
  1,571 - 1,593
  
  PRINTS
  PR01483 (FASYNTHASE)
  
  1,822 - 1,841
  
  PRINTS
  PR01483 (FASYNTHASE)
  
  1,700 - 1,723
  
  PRINTS
  PR01483 (FASYNTHASE)
IPR013565

Domain of unknown function DUF1729
1. PF08354 (DUF1729)
  
  1,030 - 1,086
  
  Pfam
  PF08354 (DUF1729)
IPR029069

HotDog domain
1. SSF54637 (Thioester...)
  
  1,570 - 1,682
  
  SUPERFAMILY
  SSF54637 (Thioesterase/thiol ester dehydrase-isomerase)
  
  1,305 - 1,436
  
  SUPERFAMILY
  SSF54637 (Thioesterase/thiol ester dehydrase-isomerase)
IPR002539

MaoC-like domain
1. PF01575 (MaoC_dehyd...)
  
  1,545 - 1,668
  
  Pfam
  PF01575 (MaoC_dehydratas)
IPR014043

Acyl transferase
1. PF00698 (Acyl_transf_1)
  
  1,689 - 2,062
  
  Pfam
  PF00698 (Acyl_transf_1)
IPR020801

Polyketide synthase, acyl transferase domain
1. SM00827 (PKS_AT)
  
  1,690 - 2,044
  
  SMART
  SM00827 (PKS_AT)

no IPR

Unintegrated signatures

G3DSA:3.10.12...

1,309 - 1,453

GENE3D
G3DSA:3.10.129.10 (G3DSA:3.10.129.10)

1,567 - 1,685

GENE3D
G3DSA:3.10.129.10 (G3DSA:3.10.129.10)
G3DSA:3.40.36...

1,686 - 1,981

GENE3D
G3DSA:3.40.366.10 (G3DSA:3.40.366.10)

162 - 535

GENE3D
G3DSA:3.40.366.10 (G3DSA:3.40.366.10)
PF13452 (MaoC_dehyd...)

1,302 - 1,426

Pfam
PF13452 (MaoC_dehydrat_N)
SSF51395 (FMN-linke...)

584 - 831

SUPERFAMILY
SSF51395 (FMN-linked oxidoreductases)
cd03447 (FAS_MaoC)

1,560 - 1,685

CDD
cd03447 (FAS_MaoC)

Residue annotation

putative active si...

1,578A

putative active site
1,578A, 1,583D, 1,585N, 1,588H, 1,603I, 1,606G, 1,607M
CDD
cd03447 (FAS_MaoC)

1,583D

putative active site
1,578A, 1,583D, 1,585N, 1,588H, 1,603I, 1,606G, 1,607M
CDD
cd03447 (FAS_MaoC)

1,585N

putative active site
1,578A, 1,583D, 1,585N, 1,588H, 1,603I, 1,606G, 1,607M
CDD
cd03447 (FAS_MaoC)

1,588H

putative active site
1,578A, 1,583D, 1,585N, 1,588H, 1,603I, 1,606G, 1,607M
CDD
cd03447 (FAS_MaoC)

1,603I

putative active site
1,578A, 1,583D, 1,585N, 1,588H, 1,603I, 1,606G, 1,607M
CDD
cd03447 (FAS_MaoC)

1,606G

putative active site
1,578A, 1,583D, 1,585N, 1,588H, 1,603I, 1,606G, 1,607M
CDD
cd03447 (FAS_MaoC)

1,607M

putative active site
1,578A, 1,583D, 1,585N, 1,588H, 1,603I, 1,606G, 1,607M
CDD
cd03447 (FAS_MaoC)
putative catalytic...

1,583D

putative catalytic site
1,583D, 1,585N, 1,588H, 1,606G
CDD
cd03447 (FAS_MaoC)

1,585N

putative catalytic site
1,583D, 1,585N, 1,588H, 1,606G
CDD
cd03447 (FAS_MaoC)

1,588H

putative catalytic site
1,583D, 1,585N, 1,588H, 1,606G
CDD
cd03447 (FAS_MaoC)

1,606G

putative catalytic site
1,583D, 1,585N, 1,588H, 1,606G
CDD
cd03447 (FAS_MaoC)

Protein sequence

>MIA_05440_1
MQPVTGVSTPHSAASLRPINLAHGSIEHTLLVPTSLYLNFTQIRDQFYTSLPPPTDDFVADDEPASPTELLASFLGFVAK
TVVDEPGPYDEVLALALTEFETRFLRGNNVHAVATALQASPDSNTTTAKVKNVVRSYYAARLAANRPIKSHESALFRAIE
DGQASIYAIFGGQGNSEDYFQELVEIYSVYGDLISDFIQLLADKILSLFRTHKDAKKIYTNGFDIMKWLKSPDSTPDVDY
LVFAPVSVPIIGIIQLAHFAITAKVLGKTPGEIRKYFSGATGHSQGLISALAIGSSESWESFEKEALKAITILFYIGFYA
QRFYPITSLTPTILEDSVAEGEGKPSPMLSVRDLSRSQLESFIKDTNKHLPKEKHIGISLVNGARNMVVTGPPQSLYGLN
LALRKAKAPSGLNQSRVPHSQRKLRITNKFLPITSPFHSHLLKDATPLVLEALRDAKVSYVSKDIKIPVYDTFDGSDLRD
SKEPIIDRIIELINFYPVNWEVATKFTSTHIVDFGPGGVSGLGPLTYKNKEGTGVRIILGGVLDGSLNNEEYGYKQELFD
RDSRAVKFAIDWVKEYSPKLISASNNIFVDTKFSRLLGRPPIMVPGMTPTTIHPEFVASTLNAGYHIELAGGGYFDPKVM
TKAIEKVEKSTSPGVGITINLIYVNPFMMNWGIPLIKTLRAKGYPVEGLTIGAGVPSLDVANEYIRDLGLKHVSFKPGST
DAINAVIAIAAANPTFPVVLQWTGGRGGGHHSFEDFHAPILQTYARIRRQPNIILVAGSGFGSHTDTYPYLTGEWSTAYH
YPPMPFDGVLFGSRVMVAKEAFTSPDAKTAIVDAPGVEDKDWERTYKVPTGGVITVNSEMGEPIHKLATRGVLFWHELDN
TIFNLPKPKRLEALKSKRAYIIKRLNDDFQKTWFGRNAEGNTVDLEDMTYAEVVSRCVELCYVKKESRWIDVSLRNFTGD
VIRRLEERFTSEAGQQAVLQSFAQLDEPFKLLDSLFAKYPESTKQLINAQDKDHFLILCRRPTQKPVPFVPVLDDNFEFY
FKKDSLWQSEDLAAVVGEDVGRTCILQGPVAVRWSTEKDVPVKDILDSVHNGLIESLLKDLYANDKKKIPTTEAFGLPAS
QSSSDIEVGSNVIVSPQGSSVSYKIASNETFLPSINDWIDLIVADKPGWFKALLTTDIIVQDTKHRPNQIRQLFTPIQGL
NVDIANADSDKLTVTVSELIHGKRTNVITVSKTASDTIKVELIEERTFERAPVALELLYQYRPEFGFAPIAEVMTDRNTR
IKNFYWRVWFGSKESINLDIDVTKPIPGDNTVVDVKAIADFVHAVGNNGEAYVSRPGKTTLAPLDFAIVAGWKAIIKAIF
PKVIDGDILKLVHLSNSFNMVPGAEPLKEGDAVVSNAILKSVVNQETGKVVSVTGVISREGKPVMEVTSEFFYRGDYNDF
ENTFERKNEAPIQISLQTPKDVAVLCSKEWFQLDNTDIELLGQTLTFRTESFVKYKNNTVFSSVETTGQVLLELPSKEII
QIGTVEYEAGESHGNPVIDYLERNGKTIELPVYFENAIPLNGGTELILRSPASNEPYANVSGDYNPIHVSRIFSKYANLP
GTITHGMYSSAAVRSLVEVWAAENNASRVRAFKCSFVGMVLPNDDIQVKLEHVGMINGRKIVKVECINVESQSPVLTGEA
EVEQPTSSYVFTGQGSQEQGMGMDLYESSPVAREVWDRADKHFVNNYGFSIVDIVKNNPKELTVHFGGPRGNAIRENYIS
MMFESIDESGQIKSEKIFKDITENTDFYTFKSPTGLLSATQFTQPALTLMEKASFEDMKSKGLVAANSSFAGHSLGEYSA
LASLGDVMPIESLVEVVFYRGMTMQVAVPRDSQGRSNYGMCAVNPSRISPTFDDAALRFVVDHISQQTKWLLEIVNYNVE
NTQYVAAGDLRALDTLTNVLNFLKIQKIDIVKLQQQLSVEKVKEHLTEIVEDVAQKSLAKPQPIDLERGFAVIPLKGISV
PFHSSYLRGGVKPFQRFLVKKIPQNAVKPANLINKYIPNLTAKPFEITKEYFEEVYKLTGSDKIKSVLDNWEKYESS

GO term prediction

Biological Process

GO:0006633 fatty acid biosynthetic process
GO:0008152 metabolic process
GO:0055114 oxidation-reduction process

Molecular Function

GO:0003824 catalytic activity
GO:0004312 fatty acid synthase activity
GO:0004318 enoyl-[acyl-carrier-protein] reductase (NADH) activity

Cellular Component

GO:0005835 fatty acid synthase complex

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Entry type

Status

Per-residue features