MIA_05378_1

Protein

Length

1,399 amino acids

Protein function

EGGNOG:	0PIYP	LYS2	L-aminoadipate-semialdehyde dehydrogenase, large subunit
SGD closest match:	S000000319	LYS2	L-2-aminoadipate reductase
CGD closest match:	CAL0000183678	LYS2	L-aminoadipate-semialdehyde dehydrogenase

Protein alignments

	%id	Aln length
MCA_02602_1	75.730%	1405	MCA_02602_1
A0A0J9XK20_GEOCN	63.983%	1416	Similar to Saccharomyces cerevisiae YBR115C LYS2 Alpha aminoadipate reductase OS=Geotrichum candidum GN=BN980_GECA27s00912g PE=4 SV=1
A0A1E3PQM7_9ASCO	61.435%	1408	L-aminoadipate-semialdehyde dehydrogenase OS=Nadsonia fulvescens var. elongata DSM 6958 GN=NADFUDRAFT_80905 PE=4 SV=1
A0A167FJS3_9ASCO	60.623%	1412	L-aminoadipate-semialdehyde dehydrogenase OS=Sugiyamaella lignohabitans GN=LYS2 PE=4 SV=1
Q6C6T5_YARLI	58.645%	1417	YALI0E06457p OS=Yarrowia lipolytica (strain CLIB 122 / E 150) GN=YALI0_E06457g PE=4 SV=1
A0A060TAU4_BLAAD	58.626%	1397	ARAD1D22814p OS=Blastobotrys adeninivorans GN=GNLVRS02_ARAD1D22814g PE=4 SV=1
A0A1D8PCV7_CANAL	56.667%	1410	L-aminoadipate-semialdehyde dehydrogenase OS=Candida albicans (strain SC5314 / ATCC MYA-2876) GN=LYS2 PE=4 SV=1
UniRef50_W1Q8V3	56.085%	1405	L-aminoadipate-semialdehyde dehydrogenase large subunit n=3 Tax=Saccharomycetales TaxID=4892 RepID=W1Q8V3_OGAPD
A0A1E4TLC7_9ASCO	56.112%	1399	Uncharacterized protein OS=Tortispora caseinolytica NRRL Y-17796 GN=CANCADRAFT_1144 PE=4 SV=1
LYS2_YEAST	53.551%	1408	L-2-aminoadipate reductase OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=LYS2 PE=1 SV=2

Mitochondrial localization by mitoprotII

Probability of mitochondrial location: 0.2517

Protein family membership

L-2-aminoadipate reductase (IPR014397)

Domains and repeats

Domain
249 - 739
AMP-dependent synthetase/ligase (IPR000873)
Amino acid adenylation domain (IPR010071)
855 - 930
Phosphopantetheine binding ACP domain (IPR009081)
Polyketide synthase, phosphopantetheine-binding domain (IPR020806)
982 - 1,364
NAD(P)-binding domain (IPR016040)
Male sterility, NAD-binding (IPR013120)
Thioester reductase-like domain (IPR010080)

1 200 400 600 800 1000 1200 1399

Detailed signature matches

IPR014397

L-2-aminoadipate reductase
1. TIGR03443
  
  11 - 1,397
  
  TIGRFAMs
  TIGR03443 (TIGR03443)
IPR000873

AMP-dependent synthetase/ligase
1. PF00501 (AMP-binding)
  
  249 - 716
  
  Pfam
  PF00501 (AMP-binding)
IPR010071

Amino acid adenylation domain
1. TIGR01733
  
  279 - 739
  
  TIGRFAMs
  TIGR01733 (TIGR01733)
IPR009081

Phosphopantetheine binding ACP domain
1. PF00550 (PP-binding)
  
  863 - 926
  
  Pfam
  PF00550 (PP-binding)
2. SSF47336 (ACP-like)
  
  856 - 927
  
  SUPERFAMILY
  SSF47336 (ACP-like)
3. PS50075 (ACP_DOMAIN)
  
  855 - 927
  
  PROSITE profiles
  PS50075 (ACP_DOMAIN)
IPR020806

Polyketide synthase, phosphopantetheine-binding domain
1. SM00823 (PKS_PP)
  
  860 - 930
  
  SMART
  SM00823 (PKS_PP)
IPR016040

NAD(P)-binding domain
1. SSF51735 (NAD(P)-bi...)
  
  982 - 1,269
  
  SUPERFAMILY
  SSF51735 (NAD(P)-binding Rossmann-fold domains)
IPR013120

Male sterility, NAD-binding
1. PF07993 (NAD_binding_4)
  
  985 - 1,234
  
  Pfam
  PF07993 (NAD_binding_4)
IPR010080

Thioester reductase-like domain
1. TIGR01746
  
  982 - 1,364
  
  TIGRFAMs
  TIGR01746 (TIGR01746)
2. cd05235 (SDR_e1)
  
  982 - 1,283
  
  CDD
  cd05235 (SDR_e1)
IPR020845

AMP-binding, conserved site
1. PS00455 (AMP_BINDING)
  
  428 - 439
  
  PROSITE patterns
  PS00455 (AMP_BINDING)

no IPR

Unintegrated signatures

G3DSA:1.10.12...

867 - 933

GENE3D
G3DSA:1.10.1200.10 (G3DSA:1.10.1200.10)
G3DSA:2.30.38.10

602 - 709

GENE3D
G3DSA:2.30.38.10 (G3DSA:2.30.38.10)
G3DSA:3.30.30...

711 - 866

GENE3D
G3DSA:3.30.300.30 (G3DSA:3.30.300.30)
G3DSA:3.40.50...

981 - 1,338

GENE3D
G3DSA:3.40.50.720 (G3DSA:3.40.50.720)
G3DSA:3.40.50...

242 - 433

GENE3D
G3DSA:3.40.50.980 (G3DSA:3.40.50.980)

434 - 591

GENE3D
G3DSA:3.40.50.980 (G3DSA:3.40.50.980)
PIRSF001617 (Alpha-AR)

5 - 1,399

PIRSF
PIRSF001617 (Alpha-AR)
SSF52777 (CoA-depen...)

5 - 213

SUPERFAMILY
SSF52777 (CoA-dependent acyltransferases)
SSF56801 (Acetyl-Co...)

792 - 845

SUPERFAMILY
SSF56801 (Acetyl-CoA synthetase-like)

686 - 759

SUPERFAMILY
SSF56801 (Acetyl-CoA synthetase-like)

209 - 658

SUPERFAMILY
SSF56801 (Acetyl-CoA synthetase-like)
cd05930 (A_NRPS)

257 - 832

CDD
cd05930 (A_NRPS)

Residue annotation

acyl-activating en...

428L

acyl-activating enzyme (AAE) consensus motif
428L, 431T, 432S, 433G, 434S, 435E, 436G, 438P, 439K
CDD
cd05930 (A_NRPS)

431T

acyl-activating enzyme (AAE) consensus motif
428L, 431T, 432S, 433G, 434S, 435E, 436G, 438P, 439K
CDD
cd05930 (A_NRPS)

432S

acyl-activating enzyme (AAE) consensus motif
428L, 431T, 432S, 433G, 434S, 435E, 436G, 438P, 439K
CDD
cd05930 (A_NRPS)

433G

acyl-activating enzyme (AAE) consensus motif
428L, 431T, 432S, 433G, 434S, 435E, 436G, 438P, 439K
CDD
cd05930 (A_NRPS)

434S

acyl-activating enzyme (AAE) consensus motif
428L, 431T, 432S, 433G, 434S, 435E, 436G, 438P, 439K
CDD
cd05930 (A_NRPS)

435E

acyl-activating enzyme (AAE) consensus motif
428L, 431T, 432S, 433G, 434S, 435E, 436G, 438P, 439K
CDD
cd05930 (A_NRPS)

436G

acyl-activating enzyme (AAE) consensus motif
428L, 431T, 432S, 433G, 434S, 435E, 436G, 438P, 439K
CDD
cd05930 (A_NRPS)

438P

acyl-activating enzyme (AAE) consensus motif
428L, 431T, 432S, 433G, 434S, 435E, 436G, 438P, 439K
CDD
cd05930 (A_NRPS)

439K

acyl-activating enzyme (AAE) consensus motif
428L, 431T, 432S, 433G, 434S, 435E, 436G, 438P, 439K
CDD
cd05930 (A_NRPS)
AMP binding site c...

431T

AMP binding site
431T, 432S, 546G, 547D, 568N, 569M, 570Y, 571G, 572T, 573T, 603A, 693D, 705C, 708R, 826K
CDD
cd05930 (A_NRPS)

432S

AMP binding site
431T, 432S, 546G, 547D, 568N, 569M, 570Y, 571G, 572T, 573T, 603A, 693D, 705C, 708R, 826K
CDD
cd05930 (A_NRPS)

546G

AMP binding site
431T, 432S, 546G, 547D, 568N, 569M, 570Y, 571G, 572T, 573T, 603A, 693D, 705C, 708R, 826K
CDD
cd05930 (A_NRPS)

547D

AMP binding site
431T, 432S, 546G, 547D, 568N, 569M, 570Y, 571G, 572T, 573T, 603A, 693D, 705C, 708R, 826K
CDD
cd05930 (A_NRPS)

568N

AMP binding site
431T, 432S, 546G, 547D, 568N, 569M, 570Y, 571G, 572T, 573T, 603A, 693D, 705C, 708R, 826K
CDD
cd05930 (A_NRPS)

569M

AMP binding site
431T, 432S, 546G, 547D, 568N, 569M, 570Y, 571G, 572T, 573T, 603A, 693D, 705C, 708R, 826K
CDD
cd05930 (A_NRPS)

570Y

AMP binding site
431T, 432S, 546G, 547D, 568N, 569M, 570Y, 571G, 572T, 573T, 603A, 693D, 705C, 708R, 826K
CDD
cd05930 (A_NRPS)

571G

AMP binding site
431T, 432S, 546G, 547D, 568N, 569M, 570Y, 571G, 572T, 573T, 603A, 693D, 705C, 708R, 826K
CDD
cd05930 (A_NRPS)

572T

AMP binding site
431T, 432S, 546G, 547D, 568N, 569M, 570Y, 571G, 572T, 573T, 603A, 693D, 705C, 708R, 826K
CDD
cd05930 (A_NRPS)

573T

AMP binding site
431T, 432S, 546G, 547D, 568N, 569M, 570Y, 571G, 572T, 573T, 603A, 693D, 705C, 708R, 826K
CDD
cd05930 (A_NRPS)

603A

AMP binding site
431T, 432S, 546G, 547D, 568N, 569M, 570Y, 571G, 572T, 573T, 603A, 693D, 705C, 708R, 826K
CDD
cd05930 (A_NRPS)

693D

AMP binding site
431T, 432S, 546G, 547D, 568N, 569M, 570Y, 571G, 572T, 573T, 603A, 693D, 705C, 708R, 826K
CDD
cd05930 (A_NRPS)

705C

AMP binding site
431T, 432S, 546G, 547D, 568N, 569M, 570Y, 571G, 572T, 573T, 603A, 693D, 705C, 708R, 826K
CDD
cd05930 (A_NRPS)

708R

AMP binding site
431T, 432S, 546G, 547D, 568N, 569M, 570Y, 571G, 572T, 573T, 603A, 693D, 705C, 708R, 826K
CDD
cd05930 (A_NRPS)

826K

AMP binding site
431T, 432S, 546G, 547D, 568N, 569M, 570Y, 571G, 572T, 573T, 603A, 693D, 705C, 708R, 826K
CDD
cd05930 (A_NRPS)
putative NAD(P) bi...

987G

putative NAD(P) binding site
987G, 989T, 990G, 991Y, 992L, 1,014V, 1,015R, 1,016A, 1,076N, 1,077G, 1,078A, 1,092P, 1,115V, 1,116S, 1,117S, 1,158Y, 1,162K, 1,185P, 1,186G, 1,187Y, 1,188V
CDD
cd05235 (SDR_e1)

989T

putative NAD(P) binding site
987G, 989T, 990G, 991Y, 992L, 1,014V, 1,015R, 1,016A, 1,076N, 1,077G, 1,078A, 1,092P, 1,115V, 1,116S, 1,117S, 1,158Y, 1,162K, 1,185P, 1,186G, 1,187Y, 1,188V
CDD
cd05235 (SDR_e1)

990G

putative NAD(P) binding site
987G, 989T, 990G, 991Y, 992L, 1,014V, 1,015R, 1,016A, 1,076N, 1,077G, 1,078A, 1,092P, 1,115V, 1,116S, 1,117S, 1,158Y, 1,162K, 1,185P, 1,186G, 1,187Y, 1,188V
CDD
cd05235 (SDR_e1)

991Y

putative NAD(P) binding site
987G, 989T, 990G, 991Y, 992L, 1,014V, 1,015R, 1,016A, 1,076N, 1,077G, 1,078A, 1,092P, 1,115V, 1,116S, 1,117S, 1,158Y, 1,162K, 1,185P, 1,186G, 1,187Y, 1,188V
CDD
cd05235 (SDR_e1)

992L

putative NAD(P) binding site
987G, 989T, 990G, 991Y, 992L, 1,014V, 1,015R, 1,016A, 1,076N, 1,077G, 1,078A, 1,092P, 1,115V, 1,116S, 1,117S, 1,158Y, 1,162K, 1,185P, 1,186G, 1,187Y, 1,188V
CDD
cd05235 (SDR_e1)

1,014V

putative NAD(P) binding site
987G, 989T, 990G, 991Y, 992L, 1,014V, 1,015R, 1,016A, 1,076N, 1,077G, 1,078A, 1,092P, 1,115V, 1,116S, 1,117S, 1,158Y, 1,162K, 1,185P, 1,186G, 1,187Y, 1,188V
CDD
cd05235 (SDR_e1)

1,015R

putative NAD(P) binding site
987G, 989T, 990G, 991Y, 992L, 1,014V, 1,015R, 1,016A, 1,076N, 1,077G, 1,078A, 1,092P, 1,115V, 1,116S, 1,117S, 1,158Y, 1,162K, 1,185P, 1,186G, 1,187Y, 1,188V
CDD
cd05235 (SDR_e1)

1,016A

putative NAD(P) binding site
987G, 989T, 990G, 991Y, 992L, 1,014V, 1,015R, 1,016A, 1,076N, 1,077G, 1,078A, 1,092P, 1,115V, 1,116S, 1,117S, 1,158Y, 1,162K, 1,185P, 1,186G, 1,187Y, 1,188V
CDD
cd05235 (SDR_e1)

1,076N

putative NAD(P) binding site
987G, 989T, 990G, 991Y, 992L, 1,014V, 1,015R, 1,016A, 1,076N, 1,077G, 1,078A, 1,092P, 1,115V, 1,116S, 1,117S, 1,158Y, 1,162K, 1,185P, 1,186G, 1,187Y, 1,188V
CDD
cd05235 (SDR_e1)

1,077G

putative NAD(P) binding site
987G, 989T, 990G, 991Y, 992L, 1,014V, 1,015R, 1,016A, 1,076N, 1,077G, 1,078A, 1,092P, 1,115V, 1,116S, 1,117S, 1,158Y, 1,162K, 1,185P, 1,186G, 1,187Y, 1,188V
CDD
cd05235 (SDR_e1)

1,078A

putative NAD(P) binding site
987G, 989T, 990G, 991Y, 992L, 1,014V, 1,015R, 1,016A, 1,076N, 1,077G, 1,078A, 1,092P, 1,115V, 1,116S, 1,117S, 1,158Y, 1,162K, 1,185P, 1,186G, 1,187Y, 1,188V
CDD
cd05235 (SDR_e1)

1,092P

putative NAD(P) binding site
987G, 989T, 990G, 991Y, 992L, 1,014V, 1,015R, 1,016A, 1,076N, 1,077G, 1,078A, 1,092P, 1,115V, 1,116S, 1,117S, 1,158Y, 1,162K, 1,185P, 1,186G, 1,187Y, 1,188V
CDD
cd05235 (SDR_e1)

1,115V

putative NAD(P) binding site
987G, 989T, 990G, 991Y, 992L, 1,014V, 1,015R, 1,016A, 1,076N, 1,077G, 1,078A, 1,092P, 1,115V, 1,116S, 1,117S, 1,158Y, 1,162K, 1,185P, 1,186G, 1,187Y, 1,188V
CDD
cd05235 (SDR_e1)

1,116S

putative NAD(P) binding site
987G, 989T, 990G, 991Y, 992L, 1,014V, 1,015R, 1,016A, 1,076N, 1,077G, 1,078A, 1,092P, 1,115V, 1,116S, 1,117S, 1,158Y, 1,162K, 1,185P, 1,186G, 1,187Y, 1,188V
CDD
cd05235 (SDR_e1)

1,117S

putative NAD(P) binding site
987G, 989T, 990G, 991Y, 992L, 1,014V, 1,015R, 1,016A, 1,076N, 1,077G, 1,078A, 1,092P, 1,115V, 1,116S, 1,117S, 1,158Y, 1,162K, 1,185P, 1,186G, 1,187Y, 1,188V
CDD
cd05235 (SDR_e1)

1,158Y

putative NAD(P) binding site
987G, 989T, 990G, 991Y, 992L, 1,014V, 1,015R, 1,016A, 1,076N, 1,077G, 1,078A, 1,092P, 1,115V, 1,116S, 1,117S, 1,158Y, 1,162K, 1,185P, 1,186G, 1,187Y, 1,188V
CDD
cd05235 (SDR_e1)

1,162K

putative NAD(P) binding site
987G, 989T, 990G, 991Y, 992L, 1,014V, 1,015R, 1,016A, 1,076N, 1,077G, 1,078A, 1,092P, 1,115V, 1,116S, 1,117S, 1,158Y, 1,162K, 1,185P, 1,186G, 1,187Y, 1,188V
CDD
cd05235 (SDR_e1)

1,185P

putative NAD(P) binding site
987G, 989T, 990G, 991Y, 992L, 1,014V, 1,015R, 1,016A, 1,076N, 1,077G, 1,078A, 1,092P, 1,115V, 1,116S, 1,117S, 1,158Y, 1,162K, 1,185P, 1,186G, 1,187Y, 1,188V
CDD
cd05235 (SDR_e1)

1,186G

putative NAD(P) binding site
987G, 989T, 990G, 991Y, 992L, 1,014V, 1,015R, 1,016A, 1,076N, 1,077G, 1,078A, 1,092P, 1,115V, 1,116S, 1,117S, 1,158Y, 1,162K, 1,185P, 1,186G, 1,187Y, 1,188V
CDD
cd05235 (SDR_e1)

1,187Y

putative NAD(P) binding site
987G, 989T, 990G, 991Y, 992L, 1,014V, 1,015R, 1,016A, 1,076N, 1,077G, 1,078A, 1,092P, 1,115V, 1,116S, 1,117S, 1,158Y, 1,162K, 1,185P, 1,186G, 1,187Y, 1,188V
CDD
cd05235 (SDR_e1)

1,188V

putative NAD(P) binding site
987G, 989T, 990G, 991Y, 992L, 1,014V, 1,015R, 1,016A, 1,076N, 1,077G, 1,078A, 1,092P, 1,115V, 1,116S, 1,117S, 1,158Y, 1,162K, 1,185P, 1,186G, 1,187Y, 1,188V
CDD
cd05235 (SDR_e1)
active site cd05235

1,093N

active site
1,093N, 1,117S, 1,158Y, 1,162K
CDD
cd05235 (SDR_e1)

1,117S

active site
1,093N, 1,117S, 1,158Y, 1,162K
CDD
cd05235 (SDR_e1)

1,158Y

active site
1,093N, 1,117S, 1,158Y, 1,162K
CDD
cd05235 (SDR_e1)

1,162K

active site
1,093N, 1,117S, 1,158Y, 1,162K
CDD
cd05235 (SDR_e1)
putative substrate...

1,117S

putative substrate binding site
1,117S, 1,158Y, 1,187Y, 1,202F, 1,213L
CDD
cd05235 (SDR_e1)

1,158Y

putative substrate binding site
1,117S, 1,158Y, 1,187Y, 1,202F, 1,213L
CDD
cd05235 (SDR_e1)

1,187Y

putative substrate binding site
1,117S, 1,158Y, 1,187Y, 1,202F, 1,213L
CDD
cd05235 (SDR_e1)

1,202F

putative substrate binding site
1,117S, 1,158Y, 1,187Y, 1,202F, 1,213L
CDD
cd05235 (SDR_e1)

1,213L

putative substrate binding site
1,117S, 1,158Y, 1,187Y, 1,202F, 1,213L
CDD
cd05235 (SDR_e1)

Protein sequence

>MIA_05378_1
MSSSANSLDLWAQKLASPTLSVLPSDFSPADSKIVEATYSTQAPASFLTNLATLAKDFSTSHQAQSFDVALAAYLILLRK
LTGDEDLLVSSDLPNKSFAFVRAPLTPETTLKDLLASIGEFFALAESNPATAKAIASYVQQKENLDSPPVYSSTAFSRHA
VDESFLNNALSVYFDGQSFSFHYNSLLFKKERVEIMAAQIYKIVAELAQSPNTKCCTIPLISEKELAVLPDPTSDLHWNE
FRGAIHDIFADNAEKFPDRTCVVETANIFNESSSERVFTYKQINEASNVVAHYLIANGIQHGDVVMIYAYRGVDFVVAVM
GTLKAGATFSAIDPAYPPARQNIYLSVAKPKGLIVLEKAGTLDKLVEDYISDNLSLKARAPALRILDNGTVVGNGELNPP
PQDIFGPFQEKKSQRTNVVVGPDSNPTLSFTSGSEGIPKGVKGRHFSLTYYFPWMSQRFNLSENDKFTMLSGIAHDPIQR
DMFTPLFLGARLLVPTSDDIGTPGALANWMAKHGATVTHLTPAMGQLLSAQAVADIPTLHHAFFVGDVLTKRDCVRLQKL
AQNVAIVNMYGTTETQRSVSYFEIPSVTTDAAYLLSQKDIMPAGQGMLNVQLLVVNRNDRNQLCGVGEVGEIYVRAGGLA
EAYLELPDMTAQKFVNNWLVDNSKWTALDVKVEGKPWTNHWFGSRDRMYRTGDLGRYLPDGNVECCGRADDQVKIRGFRI
ELGEIDTHLSRHPLVRENVTLVRRNKDEEPILVSYIVPQSFDKLKEYFDNVTDEEIDHPDPMVRGLVKYRHLTKDIRDYL
KTKLPTYSVPSVIVPLIKMPLNPNGKVDKPKLPFPDTAQLTAAAKHAGEEQSNAVFTKIESIIKEVWLDVLPHPHAILPS
DSFFDIGGHSILATSMIFALRKRLTLEVPLGLIYSHPVLSDFAAHIDKLSDDNNDYNLDRPGSSEKPKSLVSDYAADAEE
LKNTLAPKYLAPSLPLAKSSFTVFLTGATGYLGSFIVRELLQRTEIPVKIIAQVRAKTIEHGFERIKNSGIAYGIWEDSY
ADRITPFIGNIEEKQFGLDDKAWAALADEVDVIIHNGALVHWVYPYSTLRGPNVVGTVNIMELCSVGKPKLFTFVSSTST
VDTDHYVELSDSIISEGGKGIPESDNLDGSKSGLGNGYGQSKWVSEKLIRYAGEKLGLRGTIVRPGYVFGDSKSGAMNTD
DFLARMMKGCVQLGSIPNIHNTVNMVPVDHVARVVVASAFHPVSNTEVSVAHVTSHPRIRFHQFLGALAVYGYGVKIEEY
VPWRVALEKFVVGEAKDNALFPLLHFVLDNLPQNTKAPELDDTNAAASLKADAEWVGKDFSAGMGIDVKQLGVYLAYLIK
VGFLDAPKQKGQEAIPEITVSEASLELLSKVGGRGGSSN

GO term prediction

Biological Process

GO:0008152 metabolic process
GO:0009085 lysine biosynthetic process

Molecular Function

GO:0003824 catalytic activity
GO:0004043 L-aminoadipate-semialdehyde dehydrogenase activity
GO:0031177 phosphopantetheine binding

Cellular Component

None predicted.

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Entry type

Status

Per-residue features