MCA_02602_1

Protein

Length

1,406 amino acids

Gene name: LYS2

Description: L-2-aminoadipate reductase

Browser: contigB:1792882-1797103-

RNA-seq: read pairs 2468, FPKM 21.7, percentile rank 43.5% (100% = highest expression)

Protein function

Annotation:		LYS2	L-2-aminoadipate reductase
KEGG:	K00143	LYS2	L-2-aminoadipate reductase [EC:1.2.1.95]
EGGNOG:	0PIYP	LYS2	L-aminoadipate-semialdehyde dehydrogenase, large subunit
SGD closest match:	S000000319	LYS2	L-2-aminoadipate reductase
CGD closest match:	CAL0000183678	LYS2	L-aminoadipate-semialdehyde dehydrogenase

Protein alignments

	%id	Aln length
MIA_05378_1	75.57%	1404	MIA_05378_1
A0A0J9XK20_GEOCN	63.64%	1419	Similar to Saccharomyces cerevisiae YBR115C LYS2 Alpha aminoadipate reductase OS=Geotrichum candidum GN=BN980_GECA27s00912g PE=4 SV=1
A0A1E3PQM7_9ASCO	61.12%	1417	L-aminoadipate-semialdehyde dehydrogenase OS=Nadsonia fulvescens var. elongata DSM 6958 GN=NADFUDRAFT_80905 PE=4 SV=1
A0A167FJS3_9ASCO	60.95%	1416	L-aminoadipate-semialdehyde dehydrogenase OS=Sugiyamaella lignohabitans GN=LYS2 PE=4 SV=1
A0A060TAU4_BLAAD	58.45%	1408	ARAD1D22814p OS=Blastobotrys adeninivorans GN=GNLVRS02_ARAD1D22814g PE=4 SV=1
Q6C6T5_YARLI	58.75%	1423	YALI0E06457p OS=Yarrowia lipolytica (strain CLIB 122 / E 150) GN=YALI0_E06457g PE=4 SV=1
UniRef50_A0A0E9NLW3	57.32%	1408	Uncharacterized protein n=12 Tax=Fungi TaxID=4751 RepID=A0A0E9NLW3_9ASCO
A0A1D8PCV7_CANAL	56.76%	1420	L-aminoadipate-semialdehyde dehydrogenase OS=Candida albicans (strain SC5314 / ATCC MYA-2876) GN=LYS2 PE=4 SV=1
A0A1E4TLC7_9ASCO	56.92%	1409	Uncharacterized protein OS=Tortispora caseinolytica NRRL Y-17796 GN=CANCADRAFT_1144 PE=4 SV=1
LYS2_YEAST	53.21%	1417	L-2-aminoadipate reductase OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=LYS2 PE=1 SV=2

Mitochondrial localization by mitoprotII

Probability of mitochondrial location: 0.1616

Protein family membership

L-2-aminoadipate reductase (IPR014397)

Domains and repeats

Domain
253 - 744
AMP-dependent synthetase/ligase (IPR000873)
Amino acid adenylation domain (IPR010071)
860 - 936
Phosphopantetheine binding ACP domain (IPR009081)
Polyketide synthase, phosphopantetheine-binding domain (IPR020806)
991 - 1,372
NAD(P)-binding domain (IPR016040)
Male sterility, NAD-binding (IPR013120)
Thioester reductase-like domain (IPR010080)

1 200 400 600 800 1000 1200 1406

Detailed signature matches

IPR014397

L-2-aminoadipate reductase
1. TIGR03443
  
  8 - 1,405
  
  TIGRFAMs
  TIGR03443 (TIGR03443)
IPR000873

AMP-dependent synthetase/ligase
1. PF00501 (AMP-binding)
  
  253 - 721
  
  Pfam
  PF00501 (AMP-binding)
IPR010071

Amino acid adenylation domain
1. TIGR01733
  
  283 - 744
  
  TIGRFAMs
  TIGR01733 (TIGR01733)
IPR009081

Phosphopantetheine binding ACP domain
1. PF00550 (PP-binding)
  
  868 - 931
  
  Pfam
  PF00550 (PP-binding)
2. SSF47336 (ACP-like)
  
  860 - 932
  
  SUPERFAMILY
  SSF47336 (ACP-like)
3. PS50075 (ACP_DOMAIN)
  
  865 - 933
  
  PROSITE profiles
  PS50075 (ACP_DOMAIN)
IPR020806

Polyketide synthase, phosphopantetheine-binding domain
1. SM00823 (PKS_PP)
  
  866 - 936
  
  SMART
  SM00823 (PKS_PP)
IPR016040

NAD(P)-binding domain
1. SSF51735 (NAD(P)-bi...)
  
  991 - 1,279
  
  SUPERFAMILY
  SSF51735 (NAD(P)-binding Rossmann-fold domains)
IPR013120

Male sterility, NAD-binding
1. PF07993 (NAD_binding_4)
  
  994 - 1,242
  
  Pfam
  PF07993 (NAD_binding_4)
IPR010080

Thioester reductase-like domain
1. TIGR01746
  
  991 - 1,372
  
  TIGRFAMs
  TIGR01746 (TIGR01746)
2. cd05235 (SDR_e1)
  
  991 - 1,291
  
  CDD
  cd05235 (SDR_e1)
IPR020845

AMP-binding, conserved site
1. PS00455 (AMP_BINDING)
  
  432 - 443
  
  PROSITE patterns
  PS00455 (AMP_BINDING)

no IPR

Unintegrated signatures

G3DSA:1.10.12...

872 - 939

GENE3D
G3DSA:1.10.1200.10 (G3DSA:1.10.1200.10)
G3DSA:2.30.38.10

606 - 714

GENE3D
G3DSA:2.30.38.10 (G3DSA:2.30.38.10)
G3DSA:3.30.30...

716 - 871

GENE3D
G3DSA:3.30.300.30 (G3DSA:3.30.300.30)
G3DSA:3.40.50...

990 - 1,340

GENE3D
G3DSA:3.40.50.720 (G3DSA:3.40.50.720)
G3DSA:3.40.50...

247 - 420

GENE3D
G3DSA:3.40.50.980 (G3DSA:3.40.50.980)

421 - 597

GENE3D
G3DSA:3.40.50.980 (G3DSA:3.40.50.980)
PIRSF001617 (Alpha-AR)

3 - 1,406

PIRSF
PIRSF001617 (Alpha-AR)
SSF52777 (CoA-depen...)

4 - 214

SUPERFAMILY
SSF52777 (CoA-dependent acyltransferases)
SSF56801 (Acetyl-Co...)

225 - 662

SUPERFAMILY
SSF56801 (Acetyl-CoA synthetase-like)

691 - 877

SUPERFAMILY
SSF56801 (Acetyl-CoA synthetase-like)
cd05930 (A_NRPS)

261 - 836

CDD
cd05930 (A_NRPS)
mobidb-lite (disord...)

939 - 958

MobiDB Lite
mobidb-lite (disorder_prediction)

Residue annotation

acyl-activating en...

432L

acyl-activating enzyme (AAE) consensus motif
432L, 435T, 436S, 437G, 438S, 439E, 440G, 442P, 443K
CDD
cd05930 (A_NRPS)

435T

acyl-activating enzyme (AAE) consensus motif
432L, 435T, 436S, 437G, 438S, 439E, 440G, 442P, 443K
CDD
cd05930 (A_NRPS)

436S

acyl-activating enzyme (AAE) consensus motif
432L, 435T, 436S, 437G, 438S, 439E, 440G, 442P, 443K
CDD
cd05930 (A_NRPS)

437G

acyl-activating enzyme (AAE) consensus motif
432L, 435T, 436S, 437G, 438S, 439E, 440G, 442P, 443K
CDD
cd05930 (A_NRPS)

438S

acyl-activating enzyme (AAE) consensus motif
432L, 435T, 436S, 437G, 438S, 439E, 440G, 442P, 443K
CDD
cd05930 (A_NRPS)

439E

acyl-activating enzyme (AAE) consensus motif
432L, 435T, 436S, 437G, 438S, 439E, 440G, 442P, 443K
CDD
cd05930 (A_NRPS)

440G

acyl-activating enzyme (AAE) consensus motif
432L, 435T, 436S, 437G, 438S, 439E, 440G, 442P, 443K
CDD
cd05930 (A_NRPS)

442P

acyl-activating enzyme (AAE) consensus motif
432L, 435T, 436S, 437G, 438S, 439E, 440G, 442P, 443K
CDD
cd05930 (A_NRPS)

443K

acyl-activating enzyme (AAE) consensus motif
432L, 435T, 436S, 437G, 438S, 439E, 440G, 442P, 443K
CDD
cd05930 (A_NRPS)
AMP binding site c...

435T

AMP binding site
435T, 436S, 550G, 551D, 572N, 573M, 574Y, 575G, 576T, 577T, 607A, 698D, 710C, 713R, 830K
CDD
cd05930 (A_NRPS)

436S

AMP binding site
435T, 436S, 550G, 551D, 572N, 573M, 574Y, 575G, 576T, 577T, 607A, 698D, 710C, 713R, 830K
CDD
cd05930 (A_NRPS)

550G

AMP binding site
435T, 436S, 550G, 551D, 572N, 573M, 574Y, 575G, 576T, 577T, 607A, 698D, 710C, 713R, 830K
CDD
cd05930 (A_NRPS)

551D

AMP binding site
435T, 436S, 550G, 551D, 572N, 573M, 574Y, 575G, 576T, 577T, 607A, 698D, 710C, 713R, 830K
CDD
cd05930 (A_NRPS)

572N

AMP binding site
435T, 436S, 550G, 551D, 572N, 573M, 574Y, 575G, 576T, 577T, 607A, 698D, 710C, 713R, 830K
CDD
cd05930 (A_NRPS)

573M

AMP binding site
435T, 436S, 550G, 551D, 572N, 573M, 574Y, 575G, 576T, 577T, 607A, 698D, 710C, 713R, 830K
CDD
cd05930 (A_NRPS)

574Y

AMP binding site
435T, 436S, 550G, 551D, 572N, 573M, 574Y, 575G, 576T, 577T, 607A, 698D, 710C, 713R, 830K
CDD
cd05930 (A_NRPS)

575G

AMP binding site
435T, 436S, 550G, 551D, 572N, 573M, 574Y, 575G, 576T, 577T, 607A, 698D, 710C, 713R, 830K
CDD
cd05930 (A_NRPS)

576T

AMP binding site
435T, 436S, 550G, 551D, 572N, 573M, 574Y, 575G, 576T, 577T, 607A, 698D, 710C, 713R, 830K
CDD
cd05930 (A_NRPS)

577T

AMP binding site
435T, 436S, 550G, 551D, 572N, 573M, 574Y, 575G, 576T, 577T, 607A, 698D, 710C, 713R, 830K
CDD
cd05930 (A_NRPS)

607A

AMP binding site
435T, 436S, 550G, 551D, 572N, 573M, 574Y, 575G, 576T, 577T, 607A, 698D, 710C, 713R, 830K
CDD
cd05930 (A_NRPS)

698D

AMP binding site
435T, 436S, 550G, 551D, 572N, 573M, 574Y, 575G, 576T, 577T, 607A, 698D, 710C, 713R, 830K
CDD
cd05930 (A_NRPS)

710C

AMP binding site
435T, 436S, 550G, 551D, 572N, 573M, 574Y, 575G, 576T, 577T, 607A, 698D, 710C, 713R, 830K
CDD
cd05930 (A_NRPS)

713R

AMP binding site
435T, 436S, 550G, 551D, 572N, 573M, 574Y, 575G, 576T, 577T, 607A, 698D, 710C, 713R, 830K
CDD
cd05930 (A_NRPS)

830K

AMP binding site
435T, 436S, 550G, 551D, 572N, 573M, 574Y, 575G, 576T, 577T, 607A, 698D, 710C, 713R, 830K
CDD
cd05930 (A_NRPS)
putative NAD(P) bi...

996G

putative NAD(P) binding site
996G, 998T, 999G, 1,000Y, 1,001L, 1,022V, 1,023R, 1,024A, 1,084N, 1,085G, 1,086A, 1,100P, 1,123V, 1,124S, 1,125S, 1,166Y, 1,170K, 1,193P, 1,194G, 1,195Y, 1,196V
CDD
cd05235 (SDR_e1)

998T

putative NAD(P) binding site
996G, 998T, 999G, 1,000Y, 1,001L, 1,022V, 1,023R, 1,024A, 1,084N, 1,085G, 1,086A, 1,100P, 1,123V, 1,124S, 1,125S, 1,166Y, 1,170K, 1,193P, 1,194G, 1,195Y, 1,196V
CDD
cd05235 (SDR_e1)

999G

putative NAD(P) binding site
996G, 998T, 999G, 1,000Y, 1,001L, 1,022V, 1,023R, 1,024A, 1,084N, 1,085G, 1,086A, 1,100P, 1,123V, 1,124S, 1,125S, 1,166Y, 1,170K, 1,193P, 1,194G, 1,195Y, 1,196V
CDD
cd05235 (SDR_e1)

1,000Y

putative NAD(P) binding site
996G, 998T, 999G, 1,000Y, 1,001L, 1,022V, 1,023R, 1,024A, 1,084N, 1,085G, 1,086A, 1,100P, 1,123V, 1,124S, 1,125S, 1,166Y, 1,170K, 1,193P, 1,194G, 1,195Y, 1,196V
CDD
cd05235 (SDR_e1)

1,001L

putative NAD(P) binding site
996G, 998T, 999G, 1,000Y, 1,001L, 1,022V, 1,023R, 1,024A, 1,084N, 1,085G, 1,086A, 1,100P, 1,123V, 1,124S, 1,125S, 1,166Y, 1,170K, 1,193P, 1,194G, 1,195Y, 1,196V
CDD
cd05235 (SDR_e1)

1,022V

putative NAD(P) binding site
996G, 998T, 999G, 1,000Y, 1,001L, 1,022V, 1,023R, 1,024A, 1,084N, 1,085G, 1,086A, 1,100P, 1,123V, 1,124S, 1,125S, 1,166Y, 1,170K, 1,193P, 1,194G, 1,195Y, 1,196V
CDD
cd05235 (SDR_e1)

1,023R

putative NAD(P) binding site
996G, 998T, 999G, 1,000Y, 1,001L, 1,022V, 1,023R, 1,024A, 1,084N, 1,085G, 1,086A, 1,100P, 1,123V, 1,124S, 1,125S, 1,166Y, 1,170K, 1,193P, 1,194G, 1,195Y, 1,196V
CDD
cd05235 (SDR_e1)

1,024A

putative NAD(P) binding site
996G, 998T, 999G, 1,000Y, 1,001L, 1,022V, 1,023R, 1,024A, 1,084N, 1,085G, 1,086A, 1,100P, 1,123V, 1,124S, 1,125S, 1,166Y, 1,170K, 1,193P, 1,194G, 1,195Y, 1,196V
CDD
cd05235 (SDR_e1)

1,084N

putative NAD(P) binding site
996G, 998T, 999G, 1,000Y, 1,001L, 1,022V, 1,023R, 1,024A, 1,084N, 1,085G, 1,086A, 1,100P, 1,123V, 1,124S, 1,125S, 1,166Y, 1,170K, 1,193P, 1,194G, 1,195Y, 1,196V
CDD
cd05235 (SDR_e1)

1,085G

putative NAD(P) binding site
996G, 998T, 999G, 1,000Y, 1,001L, 1,022V, 1,023R, 1,024A, 1,084N, 1,085G, 1,086A, 1,100P, 1,123V, 1,124S, 1,125S, 1,166Y, 1,170K, 1,193P, 1,194G, 1,195Y, 1,196V
CDD
cd05235 (SDR_e1)

1,086A

putative NAD(P) binding site
996G, 998T, 999G, 1,000Y, 1,001L, 1,022V, 1,023R, 1,024A, 1,084N, 1,085G, 1,086A, 1,100P, 1,123V, 1,124S, 1,125S, 1,166Y, 1,170K, 1,193P, 1,194G, 1,195Y, 1,196V
CDD
cd05235 (SDR_e1)

1,100P

putative NAD(P) binding site
996G, 998T, 999G, 1,000Y, 1,001L, 1,022V, 1,023R, 1,024A, 1,084N, 1,085G, 1,086A, 1,100P, 1,123V, 1,124S, 1,125S, 1,166Y, 1,170K, 1,193P, 1,194G, 1,195Y, 1,196V
CDD
cd05235 (SDR_e1)

1,123V

putative NAD(P) binding site
996G, 998T, 999G, 1,000Y, 1,001L, 1,022V, 1,023R, 1,024A, 1,084N, 1,085G, 1,086A, 1,100P, 1,123V, 1,124S, 1,125S, 1,166Y, 1,170K, 1,193P, 1,194G, 1,195Y, 1,196V
CDD
cd05235 (SDR_e1)

1,124S

putative NAD(P) binding site
996G, 998T, 999G, 1,000Y, 1,001L, 1,022V, 1,023R, 1,024A, 1,084N, 1,085G, 1,086A, 1,100P, 1,123V, 1,124S, 1,125S, 1,166Y, 1,170K, 1,193P, 1,194G, 1,195Y, 1,196V
CDD
cd05235 (SDR_e1)

1,125S

putative NAD(P) binding site
996G, 998T, 999G, 1,000Y, 1,001L, 1,022V, 1,023R, 1,024A, 1,084N, 1,085G, 1,086A, 1,100P, 1,123V, 1,124S, 1,125S, 1,166Y, 1,170K, 1,193P, 1,194G, 1,195Y, 1,196V
CDD
cd05235 (SDR_e1)

1,166Y

putative NAD(P) binding site
996G, 998T, 999G, 1,000Y, 1,001L, 1,022V, 1,023R, 1,024A, 1,084N, 1,085G, 1,086A, 1,100P, 1,123V, 1,124S, 1,125S, 1,166Y, 1,170K, 1,193P, 1,194G, 1,195Y, 1,196V
CDD
cd05235 (SDR_e1)

1,170K

putative NAD(P) binding site
996G, 998T, 999G, 1,000Y, 1,001L, 1,022V, 1,023R, 1,024A, 1,084N, 1,085G, 1,086A, 1,100P, 1,123V, 1,124S, 1,125S, 1,166Y, 1,170K, 1,193P, 1,194G, 1,195Y, 1,196V
CDD
cd05235 (SDR_e1)

1,193P

putative NAD(P) binding site
996G, 998T, 999G, 1,000Y, 1,001L, 1,022V, 1,023R, 1,024A, 1,084N, 1,085G, 1,086A, 1,100P, 1,123V, 1,124S, 1,125S, 1,166Y, 1,170K, 1,193P, 1,194G, 1,195Y, 1,196V
CDD
cd05235 (SDR_e1)

1,194G

putative NAD(P) binding site
996G, 998T, 999G, 1,000Y, 1,001L, 1,022V, 1,023R, 1,024A, 1,084N, 1,085G, 1,086A, 1,100P, 1,123V, 1,124S, 1,125S, 1,166Y, 1,170K, 1,193P, 1,194G, 1,195Y, 1,196V
CDD
cd05235 (SDR_e1)

1,195Y

putative NAD(P) binding site
996G, 998T, 999G, 1,000Y, 1,001L, 1,022V, 1,023R, 1,024A, 1,084N, 1,085G, 1,086A, 1,100P, 1,123V, 1,124S, 1,125S, 1,166Y, 1,170K, 1,193P, 1,194G, 1,195Y, 1,196V
CDD
cd05235 (SDR_e1)

1,196V

putative NAD(P) binding site
996G, 998T, 999G, 1,000Y, 1,001L, 1,022V, 1,023R, 1,024A, 1,084N, 1,085G, 1,086A, 1,100P, 1,123V, 1,124S, 1,125S, 1,166Y, 1,170K, 1,193P, 1,194G, 1,195Y, 1,196V
CDD
cd05235 (SDR_e1)
active site cd05235

1,101N

active site
1,101N, 1,125S, 1,166Y, 1,170K
CDD
cd05235 (SDR_e1)

1,125S

active site
1,101N, 1,125S, 1,166Y, 1,170K
CDD
cd05235 (SDR_e1)

1,166Y

active site
1,101N, 1,125S, 1,166Y, 1,170K
CDD
cd05235 (SDR_e1)

1,170K

active site
1,101N, 1,125S, 1,166Y, 1,170K
CDD
cd05235 (SDR_e1)
putative substrate...

1,125S

putative substrate binding site
1,125S, 1,166Y, 1,195Y, 1,210F, 1,221L
CDD
cd05235 (SDR_e1)

1,166Y

putative substrate binding site
1,125S, 1,166Y, 1,195Y, 1,210F, 1,221L
CDD
cd05235 (SDR_e1)

1,195Y

putative substrate binding site
1,125S, 1,166Y, 1,195Y, 1,210F, 1,221L
CDD
cd05235 (SDR_e1)

1,210F

putative substrate binding site
1,125S, 1,166Y, 1,195Y, 1,210F, 1,221L
CDD
cd05235 (SDR_e1)

1,221L

putative substrate binding site
1,125S, 1,166Y, 1,195Y, 1,210F, 1,221L
CDD
cd05235 (SDR_e1)

Protein sequence

>MCA_02602_1
MSEESLQKWAEKLASPTLSVIPFDFSATDDKIVEATYETEIPKSFATNLQQLASVLGKQLDLPNLEPFHIAVIAYMILLR
KLTGDEDIIVGSDNEKGQFSIFRANITAETTLGSVIDSVAQLSKLSKSDPVTLKSIVNYIQKKSNLDSAPSLFNTSFSRN
LVDDSFLDKPLSVFFDGSKLTFHYNSLLFKKERIEIMAHQLYKIVAEINSSSDILNVKCCTIPLITDIEREVLPDPTSNL
HWPDFGGAIHDIFANNADKFPERTCVVETANVFDNSSKERVFTYKQINEASNVVAHHLIAKGITHGDVVMIYAYRGVDFV
VAVMGVLKAGATFSAIDPAYPPARQNIYLSVAKPKGLIVLEKAGTLDSEVREYLDTKLNLIAEIPALRLLDDGTVVGDLS
ISDISKDPFAPQQSLKSQRTGVIVGPDSTPTLSFTSGSEGIPKGVKGRHFSLTYYFPWMSQRFNLSENDKFTMLSGIAHD
PIQRDMFTPLFLGARLLVPTSDDIGTPGALANWMARHGATVTHLTPAMGQLLSAQAVADIPSLHHAFFVGDILTKRDCVR
LQKLARNVAIVNMYGTTETQRSVSYFEIPSITNDSSFLLSQKDIMPAGQGMLNVQLLVVNRNDRNQLCAVGEVGEIYVRA
GGLAEAYLELPDMTAQKFVNNWLVKPDQWKALDAAIPDDVPWKKYWFGSRDRMYRTGDLGRYLPDGNVECCGRADDQVKI
RGFRIELGEIDTHLSRHPLVRENVTLVRRNKDEEPVLVSYIVPQSSEMIKEFFSDDVEVEESEDPMVKGLVKYRHLTKDI
REYLKTKLASYAVPTVIVPLIKMPLNPNGKVDKPALPFPDTAQLNAAARYSTAASGEASAALTKMEETVRDVWLEVLPHP
HSIESSDSFFDIGGHSILATSMIFTLRKKLATDVPLGLIYSHPTLADFAHELERLTSDDSNDYDLARPSTSDKESKNKTV
DYSGDFDTLKDTLAPSYKGISSPLSKTGEYTVFLTGSTGYLGAFIVRELLERETKIKIIAQVRAKSVEHGMERIKNSSIA
YGLWKDSYADAITPFTGSIEEKQFGLSNEEWAKLADSVDVIIHNGALVHWVYPYSTLRGPNVIGTINIMELCSLGKPKLF
SFVSSTSTVDTTHYVKLSDSIVSEGGKGIPESDDLEGSRYGLGTGYGQSKWVAEKLIRYAGDNLGLRGCIVRPGYVFGDS
KTGVMNTDDFLARMMKGCIQLGSIPNIHNTVNMVPVDHVARVVVASAFHPPEPEHVSVAHVTSHPRIRFHQYLGALATYG
YDVKLDEYVPWRVALEKYVVGQSRDNALFPLLHFVLDNLPQNTKAPELDDTNASKALSLDAEWVGKDYSAGMGIDVKQLG
IYLSYLIQIGFIEKPQKKGEAEIPEIKLSKESLEMLGNVGGRGTKA

GO term prediction

Biological Process

GO:0008152 metabolic process
GO:0009085 lysine biosynthetic process

Molecular Function

GO:0003824 catalytic activity
GO:0004043 L-aminoadipate-semialdehyde dehydrogenase activity
GO:0031177 phosphopantetheine binding

Cellular Component

None predicted.

Filter view on

Entry type

Status

Per-residue features