Protein

MIA_05264_1

Length
577 amino acids

Browser: contig07:1160405-1162139+

Protein function

EGGNOG:0PIRESPB4ATP-binding RNA helicase involved in the biogenesis of 60S ribosomal subunits. Required for the normal formation of 18S rRNA through the processing of pre-rRNAs at sites A0, A1 and A2, and the normal formation of 25S and 5.8S rRNAs through the processing of pre-rRNAs at sites C1 and C2 (By similarity)
SGD closest match:S000001894SPB4ATP-dependent rRNA helicase SPB4
CGD closest match:CAL0000185825SPB4ATP-dependent rRNA helicase SPB4

Protein alignments

%idAln lengthE-value
MCA_03808_158.772%5700.0MCA_03808_1
A0A0J9XGA5_GEOCN55.575%5830.0RNA helicase OS=Geotrichum candidum GN=BN980_GECA15s01143g PE=3 SV=1
A0A167EIP6_9ASCO55.823%4987.44e-176RNA helicase OS=Sugiyamaella lignohabitans GN=SPB4 PE=3 SV=1
A0A060T4X7_BLAAD50.085%5912.74e-166RNA helicase OS=Blastobotrys adeninivorans GN=GNLVRS02_ARAD1C06644g PE=3 SV=1
A0A1E3PH78_9ASCO48.205%5854.62e-163RNA helicase OS=Nadsonia fulvescens var. elongata DSM 6958 GN=NADFUDRAFT_52397 PE=3 SV=1
UniRef50_A0A1E3Q2R153.830%4839.73e-156RNA helicase n=4 Tax=Saccharomycetales TaxID=4892 RepID=A0A1E3Q2R1_LIPST
SPB4_YARLI45.514%6136.99e-152ATP-dependent rRNA helicase SPB4 OS=Yarrowia lipolytica (strain CLIB 122 / E 150) GN=SPB4 PE=3 SV=1
SPB4_YEAST48.163%4901.47e-138ATP-dependent rRNA helicase SPB4 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=SPB4 PE=1 SV=1
A0A1E4T9F7_9ASCO47.028%5726.19e-139RNA helicase (Fragment) OS=Tortispora caseinolytica NRRL Y-17796 GN=CANCADRAFT_14848 PE=3 SV=1
SPB4_CANAL46.429%5045.58e-129ATP-dependent rRNA helicase SPB4 OS=Candida albicans (strain SC5314 / ATCC MYA-2876) GN=SPB4 PE=3 SV=1

Mitochondrial localization by mitoprotII

Probability of mitochondrial location: 0.0296

Protein family membership

None predicted.

Domains and repeats

  1. Domain
  2. Domain
  3. Domain
1 100 200 300 400 500 577

Detailed signature matches

  1. Domain IPR014001
    Helicase superfamily 1/2, ATP-binding domain
    1. PS51192 (HELICASE_A...)
    2. SM00487 (ultradead3)
  2. Domain IPR011545
    DEAD/DEAH box helicase domain
    1. PF00270 (DEAD)
  3. Domain IPR027417
    P-loop containing nucleoside triphosphate hydrolase
    1. SSF52540 (P-loop co...)
  4. Domain IPR001650
    Helicase, C-terminal
    1. PS51194 (HELICASE_CTER)
    2. SM00490 (helicmild6)
    3. PF00271 (Helicase_C)
  5. Domain IPR025313
    Domain of unknown function DUF4217
    1. SM01178 (DUF4217_3)
    2. PF13959 (DUF4217)
  6. SITE IPR000629
    ATP-dependent RNA helicase DEAD-box, conserved site
    1. PS00039 (DEAD_ATP_H...)
Unintegrated signatures no IPR
Unintegrated signatures
  1. Coil
  2. G3DSA:3.40.50...
  3. cd00079 (HELICc)
  4. mobidb-lite (disord...)

Residue annotation

  1. nucleotide binding...
  2. ATP-binding site c...

Protein sequence

>MIA_05264_1
MTPVQASVIPLFCGNKDVVVEAVTGSGKTLAFLIPVIERIMKIEESLKPGQTHSVIISPTRELAQQIYQVLTSILKYCPD
EKLSNGKKRRKVRAQLVIGGEKTSYSDVQIYMSRIPNILIGTPGRLLELFNSPQTKLSQVDSMVLDEADRLLELGFDVSV
KTIIGMLPQQKRAGLFSATMSDAVNDIAQIGLRNPFRISVKVGGNNSQLDRRIPLSLGISYAVLTPNQKLPLLLDMIEKE
SFKKAIVYLPTCNGVNYFYHILTFLLGKNSSGEEILSHKNLHSLHGKLIPSARKKSLEKFSSSVSKSVLLTTDVAARGLD
IPDVDLVIQYDPPADYNVFLHRSGRAGRAGKKGNSVVFLNKGNEEGYVDFMAVKMVDMQEYKVDITKLPDPYANDNVRNW
ILKDRARFDMAVKAYVSYVRFYSKHTATSIFRFHSFDFVENAKGYGLFRLPGMPELKSISPDNLPKDGWLGPPVDMDEYS
YLNEKKENERQKQLKEKKKEEHLKDKKSKEKQKPLWLERKENAEKRREKRKLRHEAKLRNTQNDNSSDDEETVEDWKDLV
RERKGKRTKTDSSFDDL

GO term prediction

Biological Process

None predicted.

Molecular Function

GO:0003676 nucleic acid binding
GO:0005524 ATP binding

Cellular Component

None predicted.