MIA_04865_1

Protein

Length

2,464 amino acids

Protein function

EGGNOG:	0QDGB	BEM2	rho GTPase activating protein
SGD closest match:	S000000957	BEM2	GTPase-activating protein BEM2/IPL2
CGD closest match:	CAL0000181133	BEM2	Bem2p

Protein alignments

	%id	Aln length	E-value
A0A0J9X429_GEOCN	53.322%	1731	0.0	Similar to Saccharomyces cerevisiae YER155C BEM2 Rho GTPase activating protein (RhoGAP) involved in the control of cytoskeleton organization and cellular morphogenesis OS=Geotrichum candidum GN=BN980_GECA02s02232g PE=4 SV=1
UniRef50_A0A0J9X429	53.322%	1731	0.0	Similar to Saccharomyces cerevisiae YER155C BEM2 Rho GTPase activating protein (RhoGAP) involved in the control of cytoskeleton organization and cellular morphogenesis n=2 Tax=Geotrichum candidum TaxID=1173061 RepID=A0A0J9X429_GEOCN
MCA_03721_1	66.014%	1021	0.0	MCA_03721_1
A0A060T9Q4_BLAAD	44.368%	1163	0.0	ARAD1B01078p OS=Blastobotrys adeninivorans GN=GNLVRS02_ARAD1B01078g PE=4 SV=1
A0A167EVN4_9ASCO	43.409%	1138	0.0	Bem2p OS=Sugiyamaella lignohabitans GN=BEM2 PE=4 SV=1
A0A1E3PR96_9ASCO	39.029%	1112	0.0	Uncharacterized protein (Fragment) OS=Nadsonia fulvescens var. elongata DSM 6958 GN=NADFUDRAFT_7965 PE=4 SV=1
Q6BZP3_YARLI	38.447%	1082	0.0	YALI0F32043p OS=Yarrowia lipolytica (strain CLIB 122 / E 150) GN=YALI0_F32043g PE=4 SV=2
BEM2_YEAST	27.981%	1233	7.95e-112	GTPase-activating protein BEM2/IPL2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=BEM2 PE=1 SV=1
Q5AGW7_CANAL	24.704%	1012	4.86e-71	Bem2p OS=Candida albicans (strain SC5314 / ATCC MYA-2876) GN=BEM2 PE=4 SV=1
A0A1E4TKX8_9ASCO	38.095%	168	4.74e-23	Uncharacterized protein OS=Tortispora caseinolytica NRRL Y-17796 GN=CANCADRAFT_998 PE=4 SV=1

Mitochondrial localization by mitoprotII

Probability of mitochondrial location: 0.0411
Predicted cleavage: 16

Protein family membership

None predicted.

Domains and repeats

Domain
234 - 356
Ras guanine nucleotide exchange factor domain (IPR023578)
399 - 442
Ras guanine nucleotide exchange factor domain (IPR023578)
485 - 703
Ras guanine nucleotide exchange factor domain (IPR023578)
885 - 900
Ras guanine nucleotide exchange factor domain (IPR023578)
937 - 998
Ras guanine nucleotide exchange factor domain (IPR023578)
1,034 - 1,198
Ras guanine nucleotide exchange factor domain (IPR023578)
1,383 - 1,463
Ras guanine nucleotide exchange factor domain (IPR023578)
1,465 - 1,659
PH domain-like (IPR011993)
1,668 - 1,868
Rho GTPase activation protein (IPR008936)
Rho GTPase-activating protein domain (IPR000198)
Domain
407 - 693
Ras guanine-nucleotide exchange factors catalytic domain (IPR001895)
947 - 1,079
Ras-like guanine nucleotide exchange factor, N-terminal (IPR000651)

1 500 1000 1500 2000 2464

Detailed signature matches

IPR023578

Ras guanine nucleotide exchange factor domain
1. SSF48366 (Ras GEF)
  
  399 - 442
  
  SUPERFAMILY
  SSF48366 (Ras GEF)
  
  485 - 703
  
  SUPERFAMILY
  SSF48366 (Ras GEF)
  
  234 - 356
  
  SUPERFAMILY
  SSF48366 (Ras GEF)
  
  937 - 998
  
  SUPERFAMILY
  SSF48366 (Ras GEF)
  
  1,034 - 1,198
  
  SUPERFAMILY
  SSF48366 (Ras GEF)
  
  885 - 900
  
  SUPERFAMILY
  SSF48366 (Ras GEF)
  
  1,383 - 1,463
  
  SUPERFAMILY
  SSF48366 (Ras GEF)
IPR001895

Ras guanine-nucleotide exchange factors catalytic domain
1. PF00617 (RasGEF)
  
  493 - 598
  
  Pfam
  PF00617 (RasGEF)
2. SM00147 (RasGEF_3)
  
  413 - 681
  
  SMART
  SM00147 (RasGEF_3)
3. G3DSA:1.10.84...
  
  407 - 693
  
  GENE3D
  G3DSA:1.10.840.10 (G3DSA:1.10.840.10)
IPR000651

Ras-like guanine nucleotide exchange factor, N-terminal
1. cd06224 (REM)
  
  950 - 1,079
  
  CDD
  cd06224 (REM)
2. PF00618 (RasGEF_N)
  
  947 - 1,075
  
  Pfam
  PF00618 (RasGEF_N)
IPR011993

PH domain-like
1. G3DSA:2.30.29.30
  
  1,465 - 1,658
  
  GENE3D
  G3DSA:2.30.29.30 (G3DSA:2.30.29.30)
2. SSF50729 (PH domain...)
  
  1,602 - 1,659
  
  SUPERFAMILY
  SSF50729 (PH domain-like)
IPR008936

Rho GTPase activation protein
1. SSF48350 (GTPase ac...)
  
  1,668 - 1,868
  
  SUPERFAMILY
  SSF48350 (GTPase activation domain, GAP)
IPR000198

Rho GTPase-activating protein domain
1. SM00324 (RhoGAP_3)
  
  1,684 - 1,865
  
  SMART
  SM00324 (RhoGAP_3)
2. PF00620 (RhoGAP)
  
  1,687 - 1,842
  
  Pfam
  PF00620 (RhoGAP)
3. PS50238 (RHOGAP)
  
  1,673 - 1,868
  
  PROSITE profiles
  PS50238 (RHOGAP)

no IPR

Unintegrated signatures

Coil

2,459 - 2,464

COILS
Coil (Coil)

1,508 - 1,538

COILS
Coil (Coil)
G3DSA:1.10.55...

1,659 - 1,871

GENE3D
G3DSA:1.10.555.10 (G3DSA:1.10.555.10)
G3DSA:1.20.87...

863 - 1,429

GENE3D
G3DSA:1.20.870.10 (G3DSA:1.20.870.10)
cd00159 (RhoGAP)

1,687 - 1,862

CDD
cd00159 (RhoGAP)
mobidb-lite (disord...)

2,300 - 2,337

MobiDB Lite
mobidb-lite (disorder_prediction)

93 - 132

MobiDB Lite
mobidb-lite (disorder_prediction)

2,164 - 2,262

MobiDB Lite
mobidb-lite (disorder_prediction)

731 - 766

MobiDB Lite
mobidb-lite (disorder_prediction)

2,443 - 2,464

MobiDB Lite
mobidb-lite (disorder_prediction)

184 - 228

MobiDB Lite
mobidb-lite (disorder_prediction)

1 - 60

MobiDB Lite
mobidb-lite (disorder_prediction)

1,876 - 1,950

MobiDB Lite
mobidb-lite (disorder_prediction)

1,528 - 1,554

MobiDB Lite
mobidb-lite (disorder_prediction)

Residue annotation

GTPase interaction...

991P

GTPase interaction site
991P, 1,046I, 1,047G, 1,050E, 1,053H, 1,054L
CDD
cd06224 (REM)

1,046I

GTPase interaction site
991P, 1,046I, 1,047G, 1,050E, 1,053H, 1,054L
CDD
cd06224 (REM)

1,047G

GTPase interaction site
991P, 1,046I, 1,047G, 1,050E, 1,053H, 1,054L
CDD
cd06224 (REM)

1,050E

GTPase interaction site
991P, 1,046I, 1,047G, 1,050E, 1,053H, 1,054L
CDD
cd06224 (REM)

1,053H

GTPase interaction site
991P, 1,046I, 1,047G, 1,050E, 1,053H, 1,054L
CDD
cd06224 (REM)

1,054L

GTPase interaction site
991P, 1,046I, 1,047G, 1,050E, 1,053H, 1,054L
CDD
cd06224 (REM)
catalytic residue ...

1,709R

catalytic residue
1,709R
CDD
cd00159 (RhoGAP)
GTPase interaction...

1,709R

GTPase interaction site
1,709R, 1,748K, 1,752R, 1,827N, 1,830I, 1,831V, 1,854Q
CDD
cd00159 (RhoGAP)

1,748K

GTPase interaction site
1,709R, 1,748K, 1,752R, 1,827N, 1,830I, 1,831V, 1,854Q
CDD
cd00159 (RhoGAP)

1,752R

GTPase interaction site
1,709R, 1,748K, 1,752R, 1,827N, 1,830I, 1,831V, 1,854Q
CDD
cd00159 (RhoGAP)

1,827N

GTPase interaction site
1,709R, 1,748K, 1,752R, 1,827N, 1,830I, 1,831V, 1,854Q
CDD
cd00159 (RhoGAP)

1,830I

GTPase interaction site
1,709R, 1,748K, 1,752R, 1,827N, 1,830I, 1,831V, 1,854Q
CDD
cd00159 (RhoGAP)

1,831V

GTPase interaction site
1,709R, 1,748K, 1,752R, 1,827N, 1,830I, 1,831V, 1,854Q
CDD
cd00159 (RhoGAP)

1,854Q

GTPase interaction site
1,709R, 1,748K, 1,752R, 1,827N, 1,830I, 1,831V, 1,854Q
CDD
cd00159 (RhoGAP)

Protein sequence

>MIA_04865_1
MYPSRFPTSPPVSRSASPLQPVASAPDQPSGSNQGNNSTPANASAHHNYPHPLHHQQQQHFTDMVSLRKDGWLNKLNAQS
GLTAFAGSSLPSMSLGNSSNPSLAPSTTNGTLNASLTTSSSNHTSSSSTSSWKLTRASLKSGVLTISKPPSDLAVRAFDS
SVPSQPLSVYAAAHPDYYPLTATVTSSSSSNNTATQNAASHRHAASIAKASSSKQPSSSTIHYLGTDPHPDLEYDRGRIV
GGSDEAICHTILFGQSTSVTKTSVLLLPLLTDIVGAMEILTLYAQTPAVQVSRLRLVIETIQNSFPGMLLDNAIFTAFLR
LVESVSCHDDAVANELKRSVVLKQKSMTEILSYASHQEHIMWSVSQPSVNEALSDRLHAIMNKHEHPHRNQHPHQQPPFQ
PSSLLQEITPDLILDLNVDVFAKQIYHFHLAFSKDWSPTTDISLLFTTKYTYNRHSPLVFDSTNIHFLGNLLLDHLFTYR
GHSSSNHHPISNAYRGKIISYWINLGNALKNCGDMVGWLAIATVICSIPVLRLRSSWCYVPPDTRDRVTKEWAPVVFDLE
RRLMISDMTRKSTFHVLAPQGIGMTYPKERVVPFFGDLCVKYEEGSSYKQCEIRLNRIRTAFDRWKHYLEQIPQNDTFEP
LPEAIPILQRLLYALLANHFSAPVQTPEDVLRLSLAIEPNFFGTYLKNHYSQRLPLQTGSYMPLLFTIVLPSYRLFAKTA
LLSACSTLFTSSSSFSSKRPLRPSSSRSGNSDSKQASRNGSGLSSSAISSTSIASISSSTTLSPAMSSPNASALSTSGVA
ELDFSSRNFIAHCRSSNALLKDVGDVLNVATRIYHLSDDIILKGFDEDSATTVPMERNNLPLALPLDGSQQQQQLANEQT
LIINTTVKAANLERLVDILVLGVGDFSTFVNPDDMARYPLPLPPQSHAPGSDLSINNTIFKVDIDIHTMTFFATYRSFCS
PSVLLESFRKRFVGAQSAALSISATNKRSLPPPPQNRHQSQHMQQGHVMSQQQQQIHLDSHFPNWDPTCDADPESIDWVI
VAQIQIGVLEACHLWVSQYFSDFANDLRARDQFLDLLRNFEVELQTWKDAGVLLRDDYKYYYDTIEALHKKVRKLFIKKS
YRPIDIKKLLPSFPQADRYENLLPNGDVKSLEALVDDLDTVAAEYFSMINLRDWMEVFEILETQSVTLTGLFNYRSMGSS
NDDELVITDIFAYMESLYKASSEERLLYSFPRPLRELFKLRNHIIHYFGSQISDPTIKRDDRVARMMSILKILGIARTRM
APLDVFSTDYPSLNEDAKSFVDDDSHSSSVDQTYPISPHIASFVESAVAAAIVRPESRAFANSWLLASKEICKQFSGIFT
GQITTIDSVVPDISYTLLARRSPLTPCVGWLLERLLEIVCYIPNMCVENSNLINFDKRRYTYILLTNILETKSSLQTFEA
RFNATEGNPALQEFSKRISYIVHPTDGLYHLDRRVAREAASRELKDYPRSASKNRVFAIYVVAEAEKLKRDSRQREVIER
QAKELKKANSKMNKALSAGGSGQDASSISDRKTSKSRFGGFFKAVRPISMAFTGSFTPPTEKTIHPDDLPDIANMGDARF
KQITSIKLIDSEITHLKAARDRSLFKVTSKGTEYWFQALNETDAEEWVRYLELARKHAYFQATMSNSAKVFGVSIRIVTE
REGSFVPRVVETLLSEIEERGLEEVGIYRIPGSLASVNALKAAFDSGEDVNMKDDRWFDVNTVTGCFKLYLRELPEPLLT
YDLLDEFVACGHLGFGSTGAGTEEAIMRLHSCVARLPPANYHLLRRLVQHLNKVTQFGESNRMHAVNLAIVFSMSLLPSS
SSAAMMNADLGAMQTILKIMISSHERIFAEHLEPVLPEEETQNETIAPLRSETPVEEWGEHQQVSRQTTPRLSTPRQNTP
RLGSVTAPSPETLLQPTQSALPPPVTTSSSSSLASVLLNKKLNSSQVSLGNVPVEEDTRHYSAPLTTNTLRQARGHSMYF
EPGSVPISAAPVPILAAPVPVSSSSALPSLSVPVSEPPRVSTPVGTSLPSSVAATPVTPATQHLSQISVSPHYVTSPQPS
HMAPATPLQPTHVVIAPSPQRIVSSPLVVPTSPSPSMSSNVAGGYPNVLSSFTVPSSPGVINSSPLVEKPIEILSPAPDP
AVALTSAPNSAPTPALASVSSTSTSAPTPTHTPPSTSASESTSESALAPDSTLDSTSDSGPGPVLASTQAPAAPAVATAP
LPSQLQSQQHPSPVYTHENPTGRTGGGIVAAAIQYYNNASGAVTSTASGFGATNALSPLSVSSSSSSVSGGMGATIGSES
RPGSLQISTSSGRSSPQRSLISVVRGNGHVAALNSNSGAVLTTSKGSTVNVSGSFDENSKVGTLGIPEDSSLGVRSKRFS
SLVMGGANYHSTSSNNNGGYLDSNALDTNDSFGGVNLDNLEAVPEQELDQGVDARHVKVEDERK

GO term prediction

Biological Process

GO:0007165 signal transduction
GO:0007264 small GTPase mediated signal transduction

Molecular Function

GO:0005085 guanyl-nucleotide exchange factor activity

Cellular Component