MCA_03721_1

Protein

Length

2,331 amino acids

Gene name: BEM2

Description: GTPase-activating protein BEM2/IPL2

Browser: contigC:875465-882461+

RNA-seq: read pairs 2999, FPKM 15.9, percentile rank 35.3% (100% = highest expression)

Protein function

Annotation:		BEM2	GTPase-activating protein BEM2/IPL2
KEGG:	K19844	BEM2	GTPase-activating protein BEM2
EGGNOG:	0QDGB	BEM2	rho GTPase activating protein
SGD closest match:	S000000957	BEM2	GTPase-activating protein BEM2/IPL2
CGD closest match:	CAL0000181133	BEM2	Bem2p

Protein alignments

	%id	Aln length	E-value
A0A0J9X429_GEOCN	51.20%	2084	0.0	Similar to Saccharomyces cerevisiae YER155C BEM2 Rho GTPase activating protein (RhoGAP) involved in the control of cytoskeleton organization and cellular morphogenesis OS=Geotrichum candidum GN=BN980_GECA02s02232g PE=4 SV=1
UniRef50_A0A0J9X429	51.20%	2084	0.0	Similar to Saccharomyces cerevisiae YER155C BEM2 Rho GTPase activating protein (RhoGAP) involved in the control of cytoskeleton organization and cellular morphogenesis n=2 Tax=Geotrichum candidum TaxID=1173061 RepID=A0A0J9X429_GEOCN
A0A060T9Q4_BLAAD	46.49%	1781	0.0	ARAD1B01078p OS=Blastobotrys adeninivorans GN=GNLVRS02_ARAD1B01078g PE=4 SV=1
MIA_04865_1	65.69%	1023	0.0	MIA_04865_1
A0A167EVN4_9ASCO	42.71%	1777	0.0	Bem2p OS=Sugiyamaella lignohabitans GN=BEM2 PE=4 SV=1
Q6BZP3_YARLI	43.47%	1010	0.0	YALI0F32043p OS=Yarrowia lipolytica (strain CLIB 122 / E 150) GN=YALI0_F32043g PE=4 SV=2
A0A1E3PR96_9ASCO	41.42%	1026	0.0	Uncharacterized protein (Fragment) OS=Nadsonia fulvescens var. elongata DSM 6958 GN=NADFUDRAFT_7965 PE=4 SV=1
BEM2_YEAST	28.46%	1300	2e-124	GTPase-activating protein BEM2/IPL2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=BEM2 PE=1 SV=1
Q5AGW7_CANAL	25.51%	537	9e-41	Bem2p OS=Candida albicans (strain SC5314 / ATCC MYA-2876) GN=BEM2 PE=4 SV=1
A0A1E4TKX8_9ASCO	40.12%	162	2e-27	Uncharacterized protein OS=Tortispora caseinolytica NRRL Y-17796 GN=CANCADRAFT_998 PE=4 SV=1

Mitochondrial localization by mitoprotII

Probability of mitochondrial location: 0.0587
Predicted cleavage: 24

Protein family membership

None predicted.

Domains and repeats

Domain
38 - 225
PH domain-like (IPR011993)
550 - 828
Ras guanine-nucleotide exchange factors catalytic domain (IPR001895)
1,139 - 1,282
Ras-like guanine nucleotide exchange factor, N-terminal (IPR000651)
1,696 - 1,887
PH domain-like (IPR011993)
1,894 - 2,089
Rho GTPase activation protein (IPR008936)
Rho GTPase-activating protein domain (IPR000198)
Domain
408 - 480
Ras guanine nucleotide exchange factor domain (IPR023578)
562 - 835
Ras guanine nucleotide exchange factor domain (IPR023578)
1,083 - 1,101
Ras guanine nucleotide exchange factor domain (IPR023578)
1,141 - 1,201
Ras guanine nucleotide exchange factor domain (IPR023578)
1,237 - 1,385
Ras guanine nucleotide exchange factor domain (IPR023578)
1,432 - 1,481
Ras guanine nucleotide exchange factor domain (IPR023578)
1,611 - 1,661
Ras guanine nucleotide exchange factor domain (IPR023578)

1 500 1000 1500 2000 2331

Detailed signature matches

IPR011993

PH domain-like
1. G3DSA:2.30.29.30
  
  38 - 225
  
  GENE3D
  G3DSA:2.30.29.30 (G3DSA:2.30.29.30)
  
  1,696 - 1,887
  
  GENE3D
  G3DSA:2.30.29.30 (G3DSA:2.30.29.30)
2. SSF50729 (PH domain...)
  
  1,826 - 1,885
  
  SUPERFAMILY
  SSF50729 (PH domain-like)
IPR023578

Ras guanine nucleotide exchange factor domain
1. SSF48366 (Ras GEF)
  
  1,611 - 1,661
  
  SUPERFAMILY
  SSF48366 (Ras GEF)
  
  562 - 835
  
  SUPERFAMILY
  SSF48366 (Ras GEF)
  
  408 - 480
  
  SUPERFAMILY
  SSF48366 (Ras GEF)
  
  1,237 - 1,385
  
  SUPERFAMILY
  SSF48366 (Ras GEF)
  
  1,432 - 1,481
  
  SUPERFAMILY
  SSF48366 (Ras GEF)
  
  1,141 - 1,201
  
  SUPERFAMILY
  SSF48366 (Ras GEF)
  
  1,083 - 1,101
  
  SUPERFAMILY
  SSF48366 (Ras GEF)
IPR001895

Ras guanine-nucleotide exchange factors catalytic domain
1. PF00617 (RasGEF)
  
  634 - 740
  
  Pfam
  PF00617 (RasGEF)
2. SM00147 (RasGEF_3)
  
  564 - 825
  
  SMART
  SM00147 (RasGEF_3)
3. PS50009 (RASGEF_CAT)
  
  568 - 824
  
  PROSITE profiles
  PS50009 (RASGEF_CAT)
4. G3DSA:1.10.84...
  
  550 - 828
  
  GENE3D
  G3DSA:1.10.840.10 (G3DSA:1.10.840.10)
IPR000651

Ras-like guanine nucleotide exchange factor, N-terminal
1. cd06224 (REM)
  
  1,139 - 1,282
  
  CDD
  cd06224 (REM)
2. PF00618 (RasGEF_N)
  
  1,149 - 1,278
  
  Pfam
  PF00618 (RasGEF_N)
IPR008936

Rho GTPase activation protein
1. SSF48350 (GTPase ac...)
  
  1,894 - 2,089
  
  SUPERFAMILY
  SSF48350 (GTPase activation domain, GAP)
IPR000198

Rho GTPase-activating protein domain
1. SM00324 (RhoGAP_3)
  
  1,911 - 2,086
  
  SMART
  SM00324 (RhoGAP_3)
2. PF00620 (RhoGAP)
  
  1,914 - 2,063
  
  Pfam
  PF00620 (RhoGAP)
3. PS50238 (RHOGAP)
  
  1,900 - 2,089
  
  PROSITE profiles
  PS50238 (RHOGAP)

no IPR

Unintegrated signatures

Coil

1,738 - 1,758

COILS
Coil (Coil)
G3DSA:1.10.55...

1,890 - 2,089

GENE3D
G3DSA:1.10.555.10 (G3DSA:1.10.555.10)
G3DSA:1.20.87...

1,066 - 1,659

GENE3D
G3DSA:1.20.870.10 (G3DSA:1.20.870.10)
cd00159 (RhoGAP)

1,914 - 2,082

CDD
cd00159 (RhoGAP)
mobidb-lite (disord...)

344 - 411

MobiDB Lite
mobidb-lite (disorder_prediction)

870 - 927

MobiDB Lite
mobidb-lite (disorder_prediction)

1 - 74

MobiDB Lite
mobidb-lite (disorder_prediction)

1,037 - 1,057

MobiDB Lite
mobidb-lite (disorder_prediction)

1,740 - 1,774

MobiDB Lite
mobidb-lite (disorder_prediction)

158 - 261

MobiDB Lite
mobidb-lite (disorder_prediction)

512 - 551

MobiDB Lite
mobidb-lite (disorder_prediction)

2,272 - 2,331

MobiDB Lite
mobidb-lite (disorder_prediction)

2,122 - 2,173

MobiDB Lite
mobidb-lite (disorder_prediction)

2,200 - 2,230

MobiDB Lite
mobidb-lite (disorder_prediction)

Residue annotation

GTPase interaction...

1,141P

GTPase interaction site
1,141P, 1,245A, 1,249I, 1,250G, 1,253E, 1,256Y, 1,257L
CDD
cd06224 (REM)

1,245A

GTPase interaction site
1,141P, 1,245A, 1,249I, 1,250G, 1,253E, 1,256Y, 1,257L
CDD
cd06224 (REM)

1,249I

GTPase interaction site
1,141P, 1,245A, 1,249I, 1,250G, 1,253E, 1,256Y, 1,257L
CDD
cd06224 (REM)

1,250G

GTPase interaction site
1,141P, 1,245A, 1,249I, 1,250G, 1,253E, 1,256Y, 1,257L
CDD
cd06224 (REM)

1,253E

GTPase interaction site
1,141P, 1,245A, 1,249I, 1,250G, 1,253E, 1,256Y, 1,257L
CDD
cd06224 (REM)

1,256Y

GTPase interaction site
1,141P, 1,245A, 1,249I, 1,250G, 1,253E, 1,256Y, 1,257L
CDD
cd06224 (REM)

1,257L

GTPase interaction site
1,141P, 1,245A, 1,249I, 1,250G, 1,253E, 1,256Y, 1,257L
CDD
cd06224 (REM)
catalytic residue ...

1,936R

catalytic residue
1,936R
CDD
cd00159 (RhoGAP)
GTPase interaction...

1,936R

GTPase interaction site
1,936R, 1,975K, 1,979R, 2,048N, 2,051I, 2,052V, 2,075Q
CDD
cd00159 (RhoGAP)

1,975K

GTPase interaction site
1,936R, 1,975K, 1,979R, 2,048N, 2,051I, 2,052V, 2,075Q
CDD
cd00159 (RhoGAP)

1,979R

GTPase interaction site
1,936R, 1,975K, 1,979R, 2,048N, 2,051I, 2,052V, 2,075Q
CDD
cd00159 (RhoGAP)

2,048N

GTPase interaction site
1,936R, 1,975K, 1,979R, 2,048N, 2,051I, 2,052V, 2,075Q
CDD
cd00159 (RhoGAP)

2,051I

GTPase interaction site
1,936R, 1,975K, 1,979R, 2,048N, 2,051I, 2,052V, 2,075Q
CDD
cd00159 (RhoGAP)

2,052V

GTPase interaction site
1,936R, 1,975K, 1,979R, 2,048N, 2,051I, 2,052V, 2,075Q
CDD
cd00159 (RhoGAP)

2,075Q

GTPase interaction site
1,936R, 1,975K, 1,979R, 2,048N, 2,051I, 2,052V, 2,075Q
CDD
cd00159 (RhoGAP)

Protein sequence

>MCA_03721_1
MTHSHQHPTSSQGLPTSAGSSRAGSPLSAAPTTASTLNNQSSKYSNFASSKEALDQQSNNVNNNNTSNSSSLIRASTKES
IASVSENFHSPQHQDLNISKEGWLNKLNSSTSALSSFSSSAYSSSPSASSPWSSANQNNWRLTRAVLKDGVLTISKPPSD
LAVKSFDSSVPSQPPSNYANSHPEYYPSSSNNSILSSNTLTNTNTTSSSSSHLKRIVSSTHSTLLPGKSNRSGSHSFNGS
SKSHHQQQQHNNSNHYQNQQHPIHQLGTPTATRLFFKGNEPHIDLEYDRGKIVGGTDEAICHTILFGPSESFAKTSVLLL
PLLTDIVSAIDMFTLYSTSVSHLASSLPPSQQPRSGSVSSTVTNNNNNNNGSPPPGSPSHASIVNDTSSTLNGKSLTSPS
QSSLSSTQTNSPPNIVSRLKLVIETIQDNFPGMLLDNTIFSVFMRLVESVSYHDDTVATDLKMSVFMKQKSMTEILSYAS
HQEPIMWSGVQVNISETVTERLHFILNRYEHSNSSHDPSNVTQYNNLPTSGSTSNISLPPPPQSNHHSASQSSKFITSIT
PDLILDLDVDIFANQIYHFHLAFSKDWSPTSDISLLFSTKYNYNRHSPLVFDSTNIHFLGDLLIDHLFNISNRVDNSYRG
KILTYWINLGNALKNCGDMVGWLAIATVICSIPVLRLRGSWCYVSSEIRDRVTKEWAPVVFDLERRLMISDMSRKSTFHV
LAPQGIGITYPKERVVPFFGDLCVKYEEGSTYKQCESRLNRIRTAFERWDYYLENIPQNDTFEPLPDAVPLIQRLLYALL
AHHFEAPIMPLESILKLSLAIEPNYFGQYLKFHYSQKPALTTGSHFPLIFNSVVPSFNLLSKNSLLAATAPQQNSTKRTL
RPSSSRTGDFRSGSIRHQSISGTSTSSLSSSPINSISSPTLNSPGTHSSFPTITNYIPLTTGYAEIDNPNRQYMASVIDR
HPFVKDVRDVLNVNAKIFHIFDDIVIKTFDDFPKINSTTIDNPLSDDPSVLGSTQTIATGNNGGSRPGSIVLVEGNAAKK
SSGSGSRRVSSQFASAPSNLNSPVLPDLFDAQTAEPIEATSVSIETVVKAANLERLVDILVISVGDFSSFVNKDDLTKYL
DNAIKHSPNQISSAILKSTPPTFVIDMDIHTMTFFATYRSFCSPSVLLESFRKRFVGAQSAALSISAATSKSDDTGSVHN
APMVPNANDVKINVSHSTSHNDVHFPNWDPTCNADPETIDWRIVAQIQIGVLEACYLWVSQYFSDFANDLVARDQFLDLL
RNFEVELQTWKDAGVLLRDDYKYYYDTIEALHKKVRKLFIKKSYRPIDIKKLLPIFPVGIHYENLPHNGDIQTLESLIDD
IDCIAAEYFSMVSLRDWMEVFETLETQGADVTGFFRYRPPHSSNEDEIIVQDIYVYMETLFKASPDERTILNFPRPIREL
FRLHTNLVYYFTSQISDSTIKRDERVGRMTSILKILGICKQKMAGADIFASESNNNVLNFYSMSEESRTTENLTPIISDS
GNSIHYGSNSTTTISPHIPSFIETAVAAAVARPESRGFANSWLQASKEISKQFGGPFSGSANSIESIVPDIPISLLKRSI
NEYDGTYEGLKPLTPCVGWLVERMLEAVCYIPNMCIENPKLINFDKRRYIYNLVVNIMNMKTGLTTLDARFNGASMDTPS
LLEFSKRISYLVNPVKGLYHLDRRVARDAASRELKEYPRASSKNKVFTVYVASEVEKLKRDQRQRDTVERQAKEQKKANT
KMSKMSGESSGSSLVDRKISKSRFGGLLKAVRPISMAFSGSFTPPAEKTIHPDDLPELGFMGDARFKQVGHLKLIDCDIS
HVKSARDKTLFKVSYKGADHWFQALSESDAEDWVRSLDLARKHANLLAVMSPTSAKVFGVPVRVVCEREGTMVPRVVETL
LNEIEERGLTEVGIYRIPGSLASVNALKAAFDSGEDVNMQDDRWFDVNTVTGCFKLYLRELPEPLLTYELMDSFVSCGAY
GNTPEGISRLRSCVVKLPPANYNLLKRLVEHLFKVTEHGDENKMHAVNLAIVFSMSLLPSSSSTAMMNSELGSMQTILKT
MILSHDKIFGDYVEDQGNYEAEAPIATIDTLSSADLEDENDVTVTNTNNNNSSSNSSAHPPVHDSLSFTKSSLVQSEENA
QTPSTAPQIPTNIIQTSNDDTKFFDHESSSFINNEAGETIQHSSNTGSHQEASDNSNYEVTSGSNNNTITENSEVEKLED
AMSRSMLTIPARAASRLQAEGRNKRFSSLILSSDFSAPGSPSDRPEAIESNNDISMIQESPGKEEYNDQSTTTTDTSKKS
NRDSYTEVSAY

GO term prediction

Biological Process

GO:0007165 signal transduction
GO:0007264 small GTPase mediated signal transduction

Molecular Function

GO:0005085 guanyl-nucleotide exchange factor activity

Cellular Component