MIA_04855_1

Protein

Length

1,848 amino acids

Protein function

EGGNOG:	0QDZF		Reverse transcriptase (RNA-dependent DNA polymerase)
CGD closest match:	CAL0000185137	orf19.2669	Uncharacterized protein

Protein alignments

	%id	Aln length	E-value
UniRef50_A5DXY5	48.10%	501	4e-139	Uncharacterized protein n=2 Tax=Lodderomyces elongisporus (strain ATCC 11503 / CBS 2605 / JCM 1781 / NBRC 1676 / NRRL YB-4239) TaxID=379508 RepID=A5DXY5_LODEL
MCA_01665_1	39.11%	496	4e-99	MCA_01665_1
A0A161HJI4_9ASCO	29.49%	495	4e-58	Gag-pol fusion protein OS=Sugiyamaella lignohabitans GN=AWJ20_4398 PE=4 SV=1
YCH4_YEAST	38.38%	284	3e-60	Putative transposon Ty5-1 protein YCL074W OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=TY5A PE=5 SV=2
A0A1D8PLS1_CANAL	25.71%	560	2e-44	Uncharacterized protein OS=Candida albicans (strain SC5314 / ATCC MYA-2876) GN=orf19.2669 PE=4 SV=1

Mitochondrial localization by mitoprotII

Probability of mitochondrial location: 0.1180
Predicted cleavage: 15

Protein family membership

None predicted.

Domains and repeats

Domain
216 - 281
Zinc finger, CCHC-type (IPR001878)
690 - 741
GAG-pre-integrase domain (IPR025724)
750 - 953
Ribonuclease H-like domain (IPR012337)
Integrase, catalytic core (IPR001584)
1,333 - 1,575
Reverse transcriptase, RNA-dependent DNA polymerase (IPR013103)

1 200 400 600 800 1000 1200 1400 1600 1848

Detailed signature matches

IPR001878

Zinc finger, CCHC-type
1. SM00343 (c2hcfinal6)
  
  240 - 256
  
  SMART
  SM00343 (c2hcfinal6)
2. G3DSA:4.10.60.10
  
  216 - 281
  
  GENE3D
  G3DSA:4.10.60.10 (G3DSA:4.10.60.10)
3. SSF57756 (Retroviru...)
  
  229 - 259
  
  SUPERFAMILY
  SSF57756 (Retrovirus zinc finger-like domains)
4. PS50158 (ZF_CCHC)
  
  241 - 255
  
  PROSITE profiles
  PS50158 (ZF_CCHC)
IPR025724

GAG-pre-integrase domain
1. PF13976 (gag_pre-in...)
  
  690 - 741
  
  Pfam
  PF13976 (gag_pre-integrs)
IPR012337

Ribonuclease H-like domain
1. SSF53098 (Ribonucle...)
  
  755 - 932
  
  SUPERFAMILY
  SSF53098 (Ribonuclease H-like)
2. G3DSA:3.30.42...
  
  750 - 953
  
  GENE3D
  G3DSA:3.30.420.10 (G3DSA:3.30.420.10)
IPR001584

Integrase, catalytic core
1. PF00665 (rve)
  
  755 - 873
  
  Pfam
  PF00665 (rve)
2. PS50994 (INTEGRASE)
  
  753 - 923
  
  PROSITE profiles
  PS50994 (INTEGRASE)
IPR013103

Reverse transcriptase, RNA-dependent DNA polymerase
1. PF07727 (RVT_2)
  
  1,333 - 1,575
  
  Pfam
  PF07727 (RVT_2)

no IPR

Unintegrated signatures

CYTOPLASMIC_D... (C...)

1 - 507

PHOBIUS
CYTOPLASMIC_DOMAIN (Cytoplasmic domain)
NON_CYTOPLASM... (N...)

531 - 1,848

PHOBIUS
NON_CYTOPLASMIC_DOMAIN (Non cytoplasmic domain)
SSF56672 (DNA/RNA p...)

1,585 - 1,767

SUPERFAMILY
SSF56672 (DNA/RNA polymerases)

1,332 - 1,555

SUPERFAMILY
SSF56672 (DNA/RNA polymerases)
TMhelix

508 - 530

TMHMM
TMhelix (TMhelix)
TRANSMEMBRANE (Tran...)

508 - 530

PHOBIUS
TRANSMEMBRANE (Transmembrane region)
cd09272 (RNase_HI_R...)

1,661 - 1,799

CDD
cd09272 (RNase_HI_RT_Ty1)
mobidb-lite (disord...)

248 - 280

MobiDB Lite
mobidb-lite (disorder_prediction)

1,034 - 1,186

MobiDB Lite
mobidb-lite (disorder_prediction)

Residue annotation

active site cd09272

1,665D

active site
1,665D, 1,708E, 1,742D, 1,791D
CDD
cd09272 (RNase_HI_RT_Ty1)

1,708E

active site
1,665D, 1,708E, 1,742D, 1,791D
CDD
cd09272 (RNase_HI_RT_Ty1)

1,742D

active site
1,665D, 1,708E, 1,742D, 1,791D
CDD
cd09272 (RNase_HI_RT_Ty1)

1,791D

active site
1,665D, 1,708E, 1,742D, 1,791D
CDD
cd09272 (RNase_HI_RT_Ty1)
RNA/DNA hybrid bin...

1,665D

RNA/DNA hybrid binding site
1,665D, 1,666A, 1,667D, 1,668Y, 1,703S, 1,704S, 1,705V, 1,708E, 1,742D, 1,768V, 1,795K
CDD
cd09272 (RNase_HI_RT_Ty1)

1,666A

RNA/DNA hybrid binding site
1,665D, 1,666A, 1,667D, 1,668Y, 1,703S, 1,704S, 1,705V, 1,708E, 1,742D, 1,768V, 1,795K
CDD
cd09272 (RNase_HI_RT_Ty1)

1,667D

RNA/DNA hybrid binding site
1,665D, 1,666A, 1,667D, 1,668Y, 1,703S, 1,704S, 1,705V, 1,708E, 1,742D, 1,768V, 1,795K
CDD
cd09272 (RNase_HI_RT_Ty1)

1,668Y

RNA/DNA hybrid binding site
1,665D, 1,666A, 1,667D, 1,668Y, 1,703S, 1,704S, 1,705V, 1,708E, 1,742D, 1,768V, 1,795K
CDD
cd09272 (RNase_HI_RT_Ty1)

1,703S

RNA/DNA hybrid binding site
1,665D, 1,666A, 1,667D, 1,668Y, 1,703S, 1,704S, 1,705V, 1,708E, 1,742D, 1,768V, 1,795K
CDD
cd09272 (RNase_HI_RT_Ty1)

1,704S

RNA/DNA hybrid binding site
1,665D, 1,666A, 1,667D, 1,668Y, 1,703S, 1,704S, 1,705V, 1,708E, 1,742D, 1,768V, 1,795K
CDD
cd09272 (RNase_HI_RT_Ty1)

1,705V

RNA/DNA hybrid binding site
1,665D, 1,666A, 1,667D, 1,668Y, 1,703S, 1,704S, 1,705V, 1,708E, 1,742D, 1,768V, 1,795K
CDD
cd09272 (RNase_HI_RT_Ty1)

1,708E

RNA/DNA hybrid binding site
1,665D, 1,666A, 1,667D, 1,668Y, 1,703S, 1,704S, 1,705V, 1,708E, 1,742D, 1,768V, 1,795K
CDD
cd09272 (RNase_HI_RT_Ty1)

1,742D

RNA/DNA hybrid binding site
1,665D, 1,666A, 1,667D, 1,668Y, 1,703S, 1,704S, 1,705V, 1,708E, 1,742D, 1,768V, 1,795K
CDD
cd09272 (RNase_HI_RT_Ty1)

1,768V

RNA/DNA hybrid binding site
1,665D, 1,666A, 1,667D, 1,668Y, 1,703S, 1,704S, 1,705V, 1,708E, 1,742D, 1,768V, 1,795K
CDD
cd09272 (RNase_HI_RT_Ty1)

1,795K

RNA/DNA hybrid binding site
1,665D, 1,666A, 1,667D, 1,668Y, 1,703S, 1,704S, 1,705V, 1,708E, 1,742D, 1,768V, 1,795K
CDD
cd09272 (RNase_HI_RT_Ty1)

Protein sequence

>MIA_04855_1
MTTRVTVQQVMGRTSAYINLTSSEKLNPLNYHAWATKILLGLRAMNNGVHLYVEAGTVSPGPNESLDDLTASLDTVVHDV
ILNNISPELIEQVNDVAIRGRDLWLYLHQEYSQLQPADVISLMKSLYAVNIESGPKLVSSIRTHVATWRSIYQSLSPDSV
VAFNILASMSPNCERLIQYALEHRFDGNNADNLDINNFVRNADKWAKLLKLTGSPATLSTEAFSASSKRHGNRSRNNVLT
CFRCKKRGHTSNRCPASWDEAQAAQQDKDKDDKESKDGKSSRAWFAEAVDGFTQIVDDDSFVSYAFESKTSDVCEKFGID
CSESDDDSESELFDSSEPYNGDEDYFDYQVSESCSNSELDVVPAADPFLGCTEDPCFVSEPCVGSGDDPCDPESFIGSPI
ISFVPEPLVGSDDGVCLPCFDFLVDPVVDPSPCDIICLADPAVDPCSSVVDSLVDSVVEPFPSDVTSLADPVVDSCSFDI
DSMADPIVDPSSSVTHPTDTKKSRLSPLLYLFVFAFLLLSLILLVLLHLFPFLDSRQSVHQAFVAESAASAEAHLVVGPD
TFDFIHDTGATSHICNNISYFTNLRPTSTTIRGLGSSSAKAVGDIVVDVLGQDGNPFDRVVLRDVLYVPNIPRNLFAARR
AIAGGCQFIENLDHISAVENGTTRVHAIAMGDLYFCRFRVVLPSKPVHEVFAIEPSANLWHQRLGHASVEVLKKLSKSLS
VKPTHFEVVDSHKCDACLGGKATSLPFTGTTEKATRPLELLVSDLSGPHVATPVGHRYVLTIMDFYSRYSYVELLVKKSD
ASLAIRNYIARCESYFSGDSRGNRVSYFHSDNGGEYISKDLEQFFVSKGIKHTFTVPHTPQQNGVAERLNRTLFEKAKSM
LLYSHAPDYLWGEAVKCANYLRNRLPSRTTGGVCPLQLWTGKPPSYQHMRVFGCRANVVIADAKRESKLSSNIDPGVFVG
YSLDRTAYRVLVDSSIVEARDVYFDESQFPFMKSDKELSSNASGIAFGIGCGSGSMSGPRADCNDSGTGSSVGLNSIAGS
NSSSGSSFGSRRSLPDVTSVSGSGSSASSTSGSTGSSPSPSLSSSPRSSSSLVVHKPRSVRRILPSSSAPLESPTLPSLP
SSPILPTSDQDPSVSSPIGPSRSDLLQVGSPAGESDNSGDSGISQRGGDIGFHPDVIASSPSPPSVELEIVPLDQEPPSS
DLVKSLDQAGVSVDTKDDTQAILPKSRSRVIAVRLPSVTPAKRPSSHDFVAPPSKHRCDDSAKLAASSVVPAKRRAEQES
ISYRRLKSLRFEYDDVALLVFTDPTTFEQAMSSEDAEFWLEACVLEMSSLNRNRTWDLVDLPPGRKAINSKWVFKIKRKA
DGSIERYKARLVCIGFSQVEGIDYTETFAPVVRYETVRIVLAIAAQFGFQVHHMDVETAFLNGDLKEDIYMRQPKGFIVK
GQESKVCHLNKSLYGLKQAPLCWNEKIHGALVKLGFVRNESDYGVYTKGRGSAMVIIALYVDDLLIAGSSTHAIANTKSS
LSSMFKMKDLGLVEQFLGMRVKQSPYHITVDVCRYIVDMLEEFGMQNCSSVKTPLPTRDLSDFSESDAAADPSLYRSIIG
KLIYAANCARPDLAVAVSFLCRYMQNPKSMHMEAAKHTLRYLKGSAQLGLEYRAQKVYKLVGYSDADYAQDKQDRKSFTG
YVFILSGGPITWACRKQVSPASSSVESEYMSLSDASKECFWINQLLSLCQIPVPLPVTMFEDNQGCIALAQNPVFHRRTK
HIDVRYHVVRHYIRSGVIHLEYLDTQVMLADMFTKNLGRVKFETLRGLLGMKAVGDSATRGGVEHAMLSQTSACENCWEI
MVDGLCLF

GO term prediction

Biological Process

GO:0015074 DNA integration

Molecular Function

GO:0003676 nucleic acid binding
GO:0008270 zinc ion binding

Cellular Component