MIA_04504_1

Protein

Length

1,229 amino acids

Browser: contig06:205127-208817-

Protein function

EGGNOG:	0PGRI	PUF3	mRNA binding protein Pumilio 2
SGD closest match:	S000003936	PUF3	mRNA-binding protein PUF3
CGD closest match:	CAL0000187550	PUF3	mRNA-binding protein

Protein alignments

	%id	Aln length	E-value
A0A060T1G3_BLAAD	68.876%	347	6.22e-172	ARAD1C20284p OS=Blastobotrys adeninivorans GN=GNLVRS02_ARAD1C20284g PE=4 SV=1
UniRef50_A0A060T1G3	68.876%	347	1.54e-168	ARAD1C20284p n=1 Tax=Blastobotrys adeninivorans TaxID=409370 RepID=A0A060T1G3_BLAAD
A0A167D7D5_9ASCO	66.476%	349	4.94e-163	mRNA-binding protein PUF3 OS=Sugiyamaella lignohabitans GN=PUF3 PE=4 SV=1
A0A0J9YHB9_GEOCN	65.110%	364	3.14e-163	Similar to Saccharomyces cerevisiae YGL178W MPT5 Member of the Puf family of RNA-binding proteins OS=Geotrichum candidum GN=BN980_GECA01s02122g PE=4 SV=1
MCA_06319_1	64.470%	349	1.82e-155	MCA_06319_1
A0A1E3PMR1_9ASCO	62.286%	350	1.82e-148	ARM repeat-containing protein OS=Nadsonia fulvescens var. elongata DSM 6958 GN=NADFUDRAFT_50632 PE=4 SV=1
Q6C669_YARLI	61.850%	346	8.70e-147	YALI0E12001p OS=Yarrowia lipolytica (strain CLIB 122 / E 150) GN=YALI0_E12001g PE=4 SV=1
A0A1D8PMA5_CANAL	50.000%	358	4.25e-109	mRNA-binding protein OS=Candida albicans (strain SC5314 / ATCC MYA-2876) GN=PUF3 PE=4 SV=1
PUF3_YEAST	47.554%	368	4.18e-101	mRNA-binding protein PUF3 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=PUF3 PE=1 SV=1
A0A1E4TM35_9ASCO	41.691%	343	1.53e-86	Uncharacterized protein OS=Tortispora caseinolytica NRRL Y-17796 GN=CANCADRAFT_20765 PE=4 SV=1

Mitochondrial localization by mitoprotII

Probability of mitochondrial location: 0.2845
Predicted cleavage: 202

Protein family membership

None predicted.

Domains and repeats

Domain
464 - 693
WD40/YVTN repeat-like-containing domain (IPR015943)
875 - 1,228
Armadillo-type fold (IPR016024)
Armadillo-like helical (IPR011989)
Pumilio homology domain (IPR033133)
Pumilio, RNA binding domain (IPR033712)
Repeat
897 - 932
Pumilio RNA-binding repeat (IPR001313)
933 - 968
Pumilio RNA-binding repeat (IPR001313)
969 - 1,004
Pumilio RNA-binding repeat (IPR001313)
1,005 - 1,040
Pumilio RNA-binding repeat (IPR001313)
1,041 - 1,076
Pumilio RNA-binding repeat (IPR001313)
1,077 - 1,112
Pumilio RNA-binding repeat (IPR001313)
1,113 - 1,148
Pumilio RNA-binding repeat (IPR001313)
1,163 - 1,198
Pumilio RNA-binding repeat (IPR001313)

1 200 400 600 800 1000 1229

Detailed signature matches

IPR015943

WD40/YVTN repeat-like-containing domain
1. G3DSA:2.130.1...
  
  464 - 693
  
  GENE3D
  G3DSA:2.130.10.10 (G3DSA:2.130.10.10)
IPR016024

Armadillo-type fold
1. SSF48371 (ARM repeat)
  
  878 - 1,219
  
  SUPERFAMILY
  SSF48371 (ARM repeat)
IPR011989

Armadillo-like helical
1. G3DSA:1.25.10.10
  
  875 - 1,228
  
  GENE3D
  G3DSA:1.25.10.10 (G3DSA:1.25.10.10)
IPR033133

Pumilio homology domain
1. PS50303 (PUM_HD)
  
  877 - 1,224
  
  PROSITE profiles
  PS50303 (PUM_HD)
IPR033712

Pumilio, RNA binding domain
1. cd07920 (Pumilio)
  
  894 - 1,216
  
  CDD
  cd07920 (Pumilio)
IPR001313

Pumilio RNA-binding repeat
1. PS50302 (PUM)
  
  1,005 - 1,040
  
  PROSITE profiles
  PS50302 (PUM)
  
  969 - 1,004
  
  PROSITE profiles
  PS50302 (PUM)
  
  897 - 932
  
  PROSITE profiles
  PS50302 (PUM)
  
  1,113 - 1,148
  
  PROSITE profiles
  PS50302 (PUM)
  
  1,163 - 1,198
  
  PROSITE profiles
  PS50302 (PUM)
  
  1,077 - 1,112
  
  PROSITE profiles
  PS50302 (PUM)
  
  1,041 - 1,076
  
  PROSITE profiles
  PS50302 (PUM)
  
  933 - 968
  
  PROSITE profiles
  PS50302 (PUM)
2. PF00806 (PUF)
  
  934 - 959
  
  Pfam
  PF00806 (PUF)
  
  898 - 928
  
  Pfam
  PF00806 (PUF)
  
  1,006 - 1,039
  
  Pfam
  PF00806 (PUF)
  
  970 - 1,003
  
  Pfam
  PF00806 (PUF)
  
  1,171 - 1,196
  
  Pfam
  PF00806 (PUF)
  
  1,085 - 1,111
  
  Pfam
  PF00806 (PUF)
  
  1,042 - 1,075
  
  Pfam
  PF00806 (PUF)
3. SM00025 (pum_5)
  
  969 - 1,004
  
  SMART
  SM00025 (pum_5)
  
  1,077 - 1,112
  
  SMART
  SM00025 (pum_5)
  
  933 - 968
  
  SMART
  SM00025 (pum_5)
  
  1,113 - 1,148
  
  SMART
  SM00025 (pum_5)
  
  1,005 - 1,040
  
  SMART
  SM00025 (pum_5)
  
  1,163 - 1,198
  
  SMART
  SM00025 (pum_5)
  
  1,041 - 1,076
  
  SMART
  SM00025 (pum_5)
  
  897 - 932
  
  SMART
  SM00025 (pum_5)

no IPR

Unintegrated signatures

Coil

468 - 488

COILS
Coil (Coil)
G3DSA:1.10.11...

241 - 363

GENE3D
G3DSA:1.10.110.10 (G3DSA:1.10.110.10)
mobidb-lite (disord...)

655 - 800

MobiDB Lite
mobidb-lite (disorder_prediction)

849 - 875

MobiDB Lite
mobidb-lite (disorder_prediction)

242 - 293

MobiDB Lite
mobidb-lite (disorder_prediction)

101 - 167

MobiDB Lite
mobidb-lite (disorder_prediction)

434 - 625

MobiDB Lite
mobidb-lite (disorder_prediction)

381 - 402

MobiDB Lite
mobidb-lite (disorder_prediction)

1 - 64

MobiDB Lite
mobidb-lite (disorder_prediction)

Residue annotation

RNA binding site c...

948N

RNA binding site
948N, 949Y, 952Q, 984C, 985R, 988Q, 1,021H, 1,024Q, 1,056C, 1,057R, 1,060Q, 1,089Q, 1,090Y, 1,092N, 1,093Y, 1,096Q, 1,125K, 1,126F, 1,128S, 1,129N, 1,132E, 1,175Q, 1,176F, 1,178N, 1,179Y, 1,182Q, 1,212V, 1,215H
CDD
cd07920 (Pumilio)

949Y

RNA binding site
948N, 949Y, 952Q, 984C, 985R, 988Q, 1,021H, 1,024Q, 1,056C, 1,057R, 1,060Q, 1,089Q, 1,090Y, 1,092N, 1,093Y, 1,096Q, 1,125K, 1,126F, 1,128S, 1,129N, 1,132E, 1,175Q, 1,176F, 1,178N, 1,179Y, 1,182Q, 1,212V, 1,215H
CDD
cd07920 (Pumilio)

952Q

RNA binding site
948N, 949Y, 952Q, 984C, 985R, 988Q, 1,021H, 1,024Q, 1,056C, 1,057R, 1,060Q, 1,089Q, 1,090Y, 1,092N, 1,093Y, 1,096Q, 1,125K, 1,126F, 1,128S, 1,129N, 1,132E, 1,175Q, 1,176F, 1,178N, 1,179Y, 1,182Q, 1,212V, 1,215H
CDD
cd07920 (Pumilio)

984C

RNA binding site
948N, 949Y, 952Q, 984C, 985R, 988Q, 1,021H, 1,024Q, 1,056C, 1,057R, 1,060Q, 1,089Q, 1,090Y, 1,092N, 1,093Y, 1,096Q, 1,125K, 1,126F, 1,128S, 1,129N, 1,132E, 1,175Q, 1,176F, 1,178N, 1,179Y, 1,182Q, 1,212V, 1,215H
CDD
cd07920 (Pumilio)

985R

RNA binding site
948N, 949Y, 952Q, 984C, 985R, 988Q, 1,021H, 1,024Q, 1,056C, 1,057R, 1,060Q, 1,089Q, 1,090Y, 1,092N, 1,093Y, 1,096Q, 1,125K, 1,126F, 1,128S, 1,129N, 1,132E, 1,175Q, 1,176F, 1,178N, 1,179Y, 1,182Q, 1,212V, 1,215H
CDD
cd07920 (Pumilio)

988Q

RNA binding site
948N, 949Y, 952Q, 984C, 985R, 988Q, 1,021H, 1,024Q, 1,056C, 1,057R, 1,060Q, 1,089Q, 1,090Y, 1,092N, 1,093Y, 1,096Q, 1,125K, 1,126F, 1,128S, 1,129N, 1,132E, 1,175Q, 1,176F, 1,178N, 1,179Y, 1,182Q, 1,212V, 1,215H
CDD
cd07920 (Pumilio)

1,021H

RNA binding site
948N, 949Y, 952Q, 984C, 985R, 988Q, 1,021H, 1,024Q, 1,056C, 1,057R, 1,060Q, 1,089Q, 1,090Y, 1,092N, 1,093Y, 1,096Q, 1,125K, 1,126F, 1,128S, 1,129N, 1,132E, 1,175Q, 1,176F, 1,178N, 1,179Y, 1,182Q, 1,212V, 1,215H
CDD
cd07920 (Pumilio)

1,024Q

RNA binding site
948N, 949Y, 952Q, 984C, 985R, 988Q, 1,021H, 1,024Q, 1,056C, 1,057R, 1,060Q, 1,089Q, 1,090Y, 1,092N, 1,093Y, 1,096Q, 1,125K, 1,126F, 1,128S, 1,129N, 1,132E, 1,175Q, 1,176F, 1,178N, 1,179Y, 1,182Q, 1,212V, 1,215H
CDD
cd07920 (Pumilio)

1,056C

RNA binding site
948N, 949Y, 952Q, 984C, 985R, 988Q, 1,021H, 1,024Q, 1,056C, 1,057R, 1,060Q, 1,089Q, 1,090Y, 1,092N, 1,093Y, 1,096Q, 1,125K, 1,126F, 1,128S, 1,129N, 1,132E, 1,175Q, 1,176F, 1,178N, 1,179Y, 1,182Q, 1,212V, 1,215H
CDD
cd07920 (Pumilio)

1,057R

RNA binding site
948N, 949Y, 952Q, 984C, 985R, 988Q, 1,021H, 1,024Q, 1,056C, 1,057R, 1,060Q, 1,089Q, 1,090Y, 1,092N, 1,093Y, 1,096Q, 1,125K, 1,126F, 1,128S, 1,129N, 1,132E, 1,175Q, 1,176F, 1,178N, 1,179Y, 1,182Q, 1,212V, 1,215H
CDD
cd07920 (Pumilio)

1,060Q

RNA binding site
948N, 949Y, 952Q, 984C, 985R, 988Q, 1,021H, 1,024Q, 1,056C, 1,057R, 1,060Q, 1,089Q, 1,090Y, 1,092N, 1,093Y, 1,096Q, 1,125K, 1,126F, 1,128S, 1,129N, 1,132E, 1,175Q, 1,176F, 1,178N, 1,179Y, 1,182Q, 1,212V, 1,215H
CDD
cd07920 (Pumilio)

1,089Q

RNA binding site
948N, 949Y, 952Q, 984C, 985R, 988Q, 1,021H, 1,024Q, 1,056C, 1,057R, 1,060Q, 1,089Q, 1,090Y, 1,092N, 1,093Y, 1,096Q, 1,125K, 1,126F, 1,128S, 1,129N, 1,132E, 1,175Q, 1,176F, 1,178N, 1,179Y, 1,182Q, 1,212V, 1,215H
CDD
cd07920 (Pumilio)

1,090Y

RNA binding site
948N, 949Y, 952Q, 984C, 985R, 988Q, 1,021H, 1,024Q, 1,056C, 1,057R, 1,060Q, 1,089Q, 1,090Y, 1,092N, 1,093Y, 1,096Q, 1,125K, 1,126F, 1,128S, 1,129N, 1,132E, 1,175Q, 1,176F, 1,178N, 1,179Y, 1,182Q, 1,212V, 1,215H
CDD
cd07920 (Pumilio)

1,092N

RNA binding site
948N, 949Y, 952Q, 984C, 985R, 988Q, 1,021H, 1,024Q, 1,056C, 1,057R, 1,060Q, 1,089Q, 1,090Y, 1,092N, 1,093Y, 1,096Q, 1,125K, 1,126F, 1,128S, 1,129N, 1,132E, 1,175Q, 1,176F, 1,178N, 1,179Y, 1,182Q, 1,212V, 1,215H
CDD
cd07920 (Pumilio)

1,093Y

RNA binding site
948N, 949Y, 952Q, 984C, 985R, 988Q, 1,021H, 1,024Q, 1,056C, 1,057R, 1,060Q, 1,089Q, 1,090Y, 1,092N, 1,093Y, 1,096Q, 1,125K, 1,126F, 1,128S, 1,129N, 1,132E, 1,175Q, 1,176F, 1,178N, 1,179Y, 1,182Q, 1,212V, 1,215H
CDD
cd07920 (Pumilio)

1,096Q

RNA binding site
948N, 949Y, 952Q, 984C, 985R, 988Q, 1,021H, 1,024Q, 1,056C, 1,057R, 1,060Q, 1,089Q, 1,090Y, 1,092N, 1,093Y, 1,096Q, 1,125K, 1,126F, 1,128S, 1,129N, 1,132E, 1,175Q, 1,176F, 1,178N, 1,179Y, 1,182Q, 1,212V, 1,215H
CDD
cd07920 (Pumilio)

1,125K

RNA binding site
948N, 949Y, 952Q, 984C, 985R, 988Q, 1,021H, 1,024Q, 1,056C, 1,057R, 1,060Q, 1,089Q, 1,090Y, 1,092N, 1,093Y, 1,096Q, 1,125K, 1,126F, 1,128S, 1,129N, 1,132E, 1,175Q, 1,176F, 1,178N, 1,179Y, 1,182Q, 1,212V, 1,215H
CDD
cd07920 (Pumilio)

1,126F

RNA binding site
948N, 949Y, 952Q, 984C, 985R, 988Q, 1,021H, 1,024Q, 1,056C, 1,057R, 1,060Q, 1,089Q, 1,090Y, 1,092N, 1,093Y, 1,096Q, 1,125K, 1,126F, 1,128S, 1,129N, 1,132E, 1,175Q, 1,176F, 1,178N, 1,179Y, 1,182Q, 1,212V, 1,215H
CDD
cd07920 (Pumilio)

1,128S

RNA binding site
948N, 949Y, 952Q, 984C, 985R, 988Q, 1,021H, 1,024Q, 1,056C, 1,057R, 1,060Q, 1,089Q, 1,090Y, 1,092N, 1,093Y, 1,096Q, 1,125K, 1,126F, 1,128S, 1,129N, 1,132E, 1,175Q, 1,176F, 1,178N, 1,179Y, 1,182Q, 1,212V, 1,215H
CDD
cd07920 (Pumilio)

1,129N

RNA binding site
948N, 949Y, 952Q, 984C, 985R, 988Q, 1,021H, 1,024Q, 1,056C, 1,057R, 1,060Q, 1,089Q, 1,090Y, 1,092N, 1,093Y, 1,096Q, 1,125K, 1,126F, 1,128S, 1,129N, 1,132E, 1,175Q, 1,176F, 1,178N, 1,179Y, 1,182Q, 1,212V, 1,215H
CDD
cd07920 (Pumilio)

1,132E

RNA binding site
948N, 949Y, 952Q, 984C, 985R, 988Q, 1,021H, 1,024Q, 1,056C, 1,057R, 1,060Q, 1,089Q, 1,090Y, 1,092N, 1,093Y, 1,096Q, 1,125K, 1,126F, 1,128S, 1,129N, 1,132E, 1,175Q, 1,176F, 1,178N, 1,179Y, 1,182Q, 1,212V, 1,215H
CDD
cd07920 (Pumilio)

1,175Q

RNA binding site
948N, 949Y, 952Q, 984C, 985R, 988Q, 1,021H, 1,024Q, 1,056C, 1,057R, 1,060Q, 1,089Q, 1,090Y, 1,092N, 1,093Y, 1,096Q, 1,125K, 1,126F, 1,128S, 1,129N, 1,132E, 1,175Q, 1,176F, 1,178N, 1,179Y, 1,182Q, 1,212V, 1,215H
CDD
cd07920 (Pumilio)

1,176F

RNA binding site
948N, 949Y, 952Q, 984C, 985R, 988Q, 1,021H, 1,024Q, 1,056C, 1,057R, 1,060Q, 1,089Q, 1,090Y, 1,092N, 1,093Y, 1,096Q, 1,125K, 1,126F, 1,128S, 1,129N, 1,132E, 1,175Q, 1,176F, 1,178N, 1,179Y, 1,182Q, 1,212V, 1,215H
CDD
cd07920 (Pumilio)

1,178N

RNA binding site
948N, 949Y, 952Q, 984C, 985R, 988Q, 1,021H, 1,024Q, 1,056C, 1,057R, 1,060Q, 1,089Q, 1,090Y, 1,092N, 1,093Y, 1,096Q, 1,125K, 1,126F, 1,128S, 1,129N, 1,132E, 1,175Q, 1,176F, 1,178N, 1,179Y, 1,182Q, 1,212V, 1,215H
CDD
cd07920 (Pumilio)

1,179Y

RNA binding site
948N, 949Y, 952Q, 984C, 985R, 988Q, 1,021H, 1,024Q, 1,056C, 1,057R, 1,060Q, 1,089Q, 1,090Y, 1,092N, 1,093Y, 1,096Q, 1,125K, 1,126F, 1,128S, 1,129N, 1,132E, 1,175Q, 1,176F, 1,178N, 1,179Y, 1,182Q, 1,212V, 1,215H
CDD
cd07920 (Pumilio)

1,182Q

RNA binding site
948N, 949Y, 952Q, 984C, 985R, 988Q, 1,021H, 1,024Q, 1,056C, 1,057R, 1,060Q, 1,089Q, 1,090Y, 1,092N, 1,093Y, 1,096Q, 1,125K, 1,126F, 1,128S, 1,129N, 1,132E, 1,175Q, 1,176F, 1,178N, 1,179Y, 1,182Q, 1,212V, 1,215H
CDD
cd07920 (Pumilio)

1,212V

RNA binding site
948N, 949Y, 952Q, 984C, 985R, 988Q, 1,021H, 1,024Q, 1,056C, 1,057R, 1,060Q, 1,089Q, 1,090Y, 1,092N, 1,093Y, 1,096Q, 1,125K, 1,126F, 1,128S, 1,129N, 1,132E, 1,175Q, 1,176F, 1,178N, 1,179Y, 1,182Q, 1,212V, 1,215H
CDD
cd07920 (Pumilio)

1,215H

RNA binding site
948N, 949Y, 952Q, 984C, 985R, 988Q, 1,021H, 1,024Q, 1,056C, 1,057R, 1,060Q, 1,089Q, 1,090Y, 1,092N, 1,093Y, 1,096Q, 1,125K, 1,126F, 1,128S, 1,129N, 1,132E, 1,175Q, 1,176F, 1,178N, 1,179Y, 1,182Q, 1,212V, 1,215H
CDD
cd07920 (Pumilio)

Protein sequence

>MIA_04504_1
MPQTFIQLESQHSTCPPPQQQPQPQPRPANGVFGPHHHHHHQNLHHHHPNITNKHNNNNINYHHHQPYHILPTLPHIPTP
VSPRLIHPIPVPAISLVPPSASPPTFVSSSKAPPRIHPARSPSLSPRSLAAPTTTISPPPPLSSAQPGGTPSSPPPPSSS
SVSPGNSFASTPAASSLVFTPQNFSVFAASSRLFAPPSAAAISVFSSPLSKSLVGTPQESLTASSSFIASSITAINTNTA
TSISNSSSSSSTSLSNTSPASSTCSIEKSAQPETPSSVSDFSSNPSSFSSTSSISPQLSLASLAVGPKQQQKQQQQPQLQ
QQLHPNHHTTFNLYNSTTTTPSTGSVGTPVISSSMASSSAPFAPRSASVAAASAATTSPIWTTPASLTSSPGPAITGPRH
NSVSEIDREFGELSLGYGSKSSFSGPSVGAPGLKTLPSLTSSPASGPPSKMATPTTTPFKPLSVSLHYQQQQQQQQQQQQ
QHQQQQHQQQLQSQQQMQNQPSSRRNSALSDSKFFPDFVGGSPASTEPGLRTSALSPPPHAQLQLQSSTSSQPPAQPTPH
HQQLHLNQQQPSPAFTPTPSIMSYRSSPRFNGSAVSSASSTHATLQRSAQPLPPPSQSFISSPAQPLQPANNLISLLDRF
ANIGDVTRTAELHAHSDYSSRHGSVSSIYSTGSPAPGGPSLYGGDSSYASTSPFASPVPSRLGSISGVSSISSPWQQSST
ASASTVVSPSMTTTLSPRPFDSPMVDLSMRSPLPQHQYASVFSSPETSWQSQPQLQQQPQPQQPQQLLAHPSQQPSPQQI
PLEFDQQPFRASPSSNLRPNEKTLRQQLAYQQQYPETTNYHSYPRQIQGRARSSGSISNGANGNGNAGTPLPYDSSMRSP
LLEEFRGNKQRKYELRDIYGHIVEFSGDQYGSRFIQSKLETASSEDKEVLFLELRSNALQLMTDVFGNYVIQKFFELGNQ
MQKTMLAKQMEGHVLSLSLQMYGCRVIQKAIEHILTDQQARLIKELDGHVLQCIKDQNGNHVVQKAIERIPSEHIGFILR
AFDGQVFNLATHAYGCRVIQRMLEYCDESAQQELLRDLHHYSYHLVDDQYGNYVMQHVIERGKPEDRDKIIELVRVSLLT
FSRHKFASNVVEKCIVYGTPDQRAAYIDVLLGGPADTAPPMDSAASPLSLMMKDQFANYVIQKLLECAEKPQRDRLARHV
KAQLQVLKKMSVGKHVTSIERLLDECSEV

GO term prediction

Biological Process

None predicted.

Molecular Function

GO:0003723 RNA binding
GO:0005488 binding
GO:0005515 protein binding

Cellular Component

None predicted.

Filter view on

Entry type

Status

Per-residue features

MIA_04504_1

Protein function

Protein alignments

Mitochondrial localization by mitoprotII

Protein family membership

Domains and repeats

Detailed signature matches

Residue annotation

Protein sequence

GO term prediction

Biological Process

Molecular Function

Cellular Component