MIA_04462_1

Protein

Length

519 amino acids

Protein function

EGGNOG:	0PIXI	PGUG_01925	protease
SGD closest match:	S000004111	YPS3	Aspartic proteinase yapsin-3
CGD closest match:	CAL0000189556	SAP1	Candidapepsin-1

Protein alignments

	%id	Aln length	E-value
MCA_06344_1	55.955%	445	4.13e-156	MCA_06344_1
M1XFD0_GEOCN	56.404%	445	1.56e-147	Aspartic protease OS=Geotrichum candidum GN=YSP3 PE=3 SV=1
UniRef50_M1XFD0	56.404%	445	3.18e-144	Aspartic protease n=1 Tax=Geotrichum candidum TaxID=1173061 RepID=M1XFD0_GEOCN
A0A060TDF2_BLAAD	40.330%	424	1.86e-90	ARAD1D06006p OS=Blastobotrys adeninivorans GN=GNLVRS02_ARAD1D06006g PE=3 SV=1
A0A1E3PJQ9_9ASCO	43.641%	401	2.62e-85	Acid protease OS=Nadsonia fulvescens var. elongata DSM 6958 GN=NADFUDRAFT_46336 PE=3 SV=1
A0A167DZP5_9ASCO	36.303%	449	7.62e-80	Yps1p OS=Sugiyamaella lignohabitans GN=YPS1 PE=3 SV=1
Q6C6E4_YARLI	39.953%	428	7.94e-77	YALI0E10175p OS=Yarrowia lipolytica (strain CLIB 122 / E 150) GN=YALI0_E10175g PE=3 SV=1
YPS3_YEAST	39.806%	412	5.32e-77	Aspartic proteinase yapsin-3 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=YPS3 PE=1 SV=1
CARP1_CANAL	37.121%	396	1.34e-55	Candidapepsin-1 OS=Candida albicans (strain SC5314 / ATCC MYA-2876) GN=SAP1 PE=1 SV=1
A0A1E4TI13_9ASCO	33.067%	375	4.34e-29	Uncharacterized protein OS=Tortispora caseinolytica NRRL Y-17796 GN=CANCADRAFT_123212 PE=3 SV=1

Mitochondrial localization by mitoprotII

Probability of mitochondrial location: 0.0928

Protein family membership

Aspartic peptidase A1 family (IPR001461)

Domains and repeats

Domain
52 - 492
Aspartic peptidase domain (IPR021109)
Peptidase family A1 domain (IPR033121)
Secreted aspartic endopeptidase (IPR033876)

1 50 100 150 200 250 300 350 400 450 519

Detailed signature matches

IPR001461

Aspartic peptidase A1 family
1. PR00792 (PEPSIN)
  
  388 - 403
  
  PRINTS
  PR00792 (PEPSIN)
  
  294 - 305
  
  PRINTS
  PR00792 (PEPSIN)
  
  74 - 94
  
  PRINTS
  PR00792 (PEPSIN)
  
  233 - 246
  
  PRINTS
  PR00792 (PEPSIN)
IPR021109

Aspartic peptidase domain
1. G3DSA:2.40.70.10
  
  55 - 223
  
  GENE3D
  G3DSA:2.40.70.10 (G3DSA:2.40.70.10)
  
  224 - 492
  
  GENE3D
  G3DSA:2.40.70.10 (G3DSA:2.40.70.10)
2. SSF50630 (Acid prot...)
  
  52 - 436
  
  SUPERFAMILY
  SSF50630 (Acid proteases)
IPR033121

Peptidase family A1 domain
1. PS51767 (PEPTIDASE_A1)
  
  68 - 412
  
  PROSITE profiles
  PS51767 (PEPTIDASE_A1)
2. PF00026 (Asp)
  
  68 - 414
  
  Pfam
  PF00026 (Asp)
IPR033876

Secreted aspartic endopeptidase
1. cd05474 (SAP_like)
  
  66 - 414
  
  CDD
  cd05474 (SAP_like)
IPR001969

Aspartic peptidase, active site
1. PS00141 (ASP_PROTEASE)
  
  83 - 94
  
  PROSITE patterns
  PS00141 (ASP_PROTEASE)
  
  294 - 305
  
  PROSITE patterns
  PS00141 (ASP_PROTEASE)

no IPR

Unintegrated signatures

CYTOPLASMIC_D... (C...)

519 - 519

PHOBIUS
CYTOPLASMIC_DOMAIN (Cytoplasmic domain)
NON_CYTOPLASM... (N...)

22 - 499

PHOBIUS
NON_CYTOPLASMIC_DOMAIN (Non cytoplasmic domain)
SIGNAL_PEPTIDE (Sig...)

1 - 21

PHOBIUS
SIGNAL_PEPTIDE (Signal Peptide)
SIGNAL_PEPTID... (S...)

16 - 21

PHOBIUS
SIGNAL_PEPTIDE_C_REGION (Signal peptide C-region)
SIGNAL_PEPTID... (S...)

4 - 15

PHOBIUS
SIGNAL_PEPTIDE_H_REGION (Signal peptide H-region)
SIGNAL_PEPTID... (S...)

1 - 3

PHOBIUS
SIGNAL_PEPTIDE_N_REGION (Signal peptide N-region)
SignalP-noTM

1 - 21

SignalP euk
SignalP-noTM (SignalP-noTM)
TMhelix

496 - 518

TMHMM
TMhelix (TMhelix)
TRANSMEMBRANE (Tran...)

500 - 518

PHOBIUS
TRANSMEMBRANE (Transmembrane region)

Residue annotation

inhibitor binding ...

67Y

inhibitor binding site
67Y, 139F, 142K, 147T, 153W, 188A, 381L, 387F
CDD
cd05474 (SAP_like)

139F

inhibitor binding site
67Y, 139F, 142K, 147T, 153W, 188A, 381L, 387F
CDD
cd05474 (SAP_like)

142K

inhibitor binding site
67Y, 139F, 142K, 147T, 153W, 188A, 381L, 387F
CDD
cd05474 (SAP_like)

147T

inhibitor binding site
67Y, 139F, 142K, 147T, 153W, 188A, 381L, 387F
CDD
cd05474 (SAP_like)

153W

inhibitor binding site
67Y, 139F, 142K, 147T, 153W, 188A, 381L, 387F
CDD
cd05474 (SAP_like)

188A

inhibitor binding site
67Y, 139F, 142K, 147T, 153W, 188A, 381L, 387F
CDD
cd05474 (SAP_like)

381L

inhibitor binding site
67Y, 139F, 142K, 147T, 153W, 188A, 381L, 387F
CDD
cd05474 (SAP_like)

387F

inhibitor binding site
67Y, 139F, 142K, 147T, 153W, 188A, 381L, 387F
CDD
cd05474 (SAP_like)
catalytic residue ...

86D

catalytic residue
86D, 297D
CDD
cd05474 (SAP_like)

297D

catalytic residue
86D, 297D
CDD
cd05474 (SAP_like)
catalytic motif cd...

86D

catalytic motif
86D, 87T, 88G, 89S, 297D, 298S, 299G, 300T
CDD
cd05474 (SAP_like)

87T

catalytic motif
86D, 87T, 88G, 89S, 297D, 298S, 299G, 300T
CDD
cd05474 (SAP_like)

88G

catalytic motif
86D, 87T, 88G, 89S, 297D, 298S, 299G, 300T
CDD
cd05474 (SAP_like)

89S

catalytic motif
86D, 87T, 88G, 89S, 297D, 298S, 299G, 300T
CDD
cd05474 (SAP_like)

297D

catalytic motif
86D, 87T, 88G, 89S, 297D, 298S, 299G, 300T
CDD
cd05474 (SAP_like)

298S

catalytic motif
86D, 87T, 88G, 89S, 297D, 298S, 299G, 300T
CDD
cd05474 (SAP_like)

299G

catalytic motif
86D, 87T, 88G, 89S, 297D, 298S, 299G, 300T
CDD
cd05474 (SAP_like)

300T

catalytic motif
86D, 87T, 88G, 89S, 297D, 298S, 299G, 300T
CDD
cd05474 (SAP_like)
Active site flap c...

140Y

Active site flap
140Y, 141I, 142K, 143Y, 144G, 145D, 147T, 148V, 149S, 150E, 151G
CDD
cd05474 (SAP_like)

141I

Active site flap
140Y, 141I, 142K, 143Y, 144G, 145D, 147T, 148V, 149S, 150E, 151G
CDD
cd05474 (SAP_like)

142K

Active site flap
140Y, 141I, 142K, 143Y, 144G, 145D, 147T, 148V, 149S, 150E, 151G
CDD
cd05474 (SAP_like)

143Y

Active site flap
140Y, 141I, 142K, 143Y, 144G, 145D, 147T, 148V, 149S, 150E, 151G
CDD
cd05474 (SAP_like)

144G

Active site flap
140Y, 141I, 142K, 143Y, 144G, 145D, 147T, 148V, 149S, 150E, 151G
CDD
cd05474 (SAP_like)

145D

Active site flap
140Y, 141I, 142K, 143Y, 144G, 145D, 147T, 148V, 149S, 150E, 151G
CDD
cd05474 (SAP_like)

147T

Active site flap
140Y, 141I, 142K, 143Y, 144G, 145D, 147T, 148V, 149S, 150E, 151G
CDD
cd05474 (SAP_like)

148V

Active site flap
140Y, 141I, 142K, 143Y, 144G, 145D, 147T, 148V, 149S, 150E, 151G
CDD
cd05474 (SAP_like)

149S

Active site flap
140Y, 141I, 142K, 143Y, 144G, 145D, 147T, 148V, 149S, 150E, 151G
CDD
cd05474 (SAP_like)

150E

Active site flap
140Y, 141I, 142K, 143Y, 144G, 145D, 147T, 148V, 149S, 150E, 151G
CDD
cd05474 (SAP_like)

151G

Active site flap
140Y, 141I, 142K, 143Y, 144G, 145D, 147T, 148V, 149S, 150E, 151G
CDD
cd05474 (SAP_like)

Protein sequence

>MIA_04462_1
MLNLPGALLLSTLATSSLALAADVAGTLQLDFAVERTSRVLNHRYGHLRRRAVSGTEEVTLQNELTYYNVEVTLGTPAQT
FNVLIDTGSSDLWVISNENPYCSSSSSSSVSSDSTFDCSTSGTFDSSASSTFNKNNSDFYIKYGDGTVSEGDWATDTFTM
GNISIDNMSFGLGSLTNSTIGILGVGYAANEASVAIKNNPYTYRNLPLRLVDSGYINTPAYSLWLNDMNAETGSILFGGV
DHAKYTGTLQTVPVLKSSTSKTYSAFLVALTEVSISSGDDSTVTSVLNGSPIQALLDSGTSLSYFPKDIANEIVMTQLQG
SYVSSLGYYVQSCNLAGNLTYNFSGVTINVPLSSVLLPLTTDTGRQVYSKGGIPVCIIGILPFSSSFALLGDTFLRNAYV
VYDLQNNQIGLAQAKLNATDSDIEVIKSTIPSATQASLYSATSSIAGLSTATYSNRLRTSIVETVSGTIALGSATDATTT
ATSTAKSAAMAVSAPSPVSMVYLMGFSLFFSAGFFCLFI

GO term prediction

Biological Process

GO:0006508 proteolysis

Molecular Function

GO:0004190 aspartic-type endopeptidase activity

Cellular Component

None predicted.

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Entry type

Status

Per-residue features