MIA_04440_1

Protein

Length

1,800 amino acids

Protein function

EGGNOG:	0QDZF		Reverse transcriptase (RNA-dependent DNA polymerase)
CGD closest match:	CAL0000185137	orf19.2669	Uncharacterized protein

Protein alignments

	%id	Aln length	E-value
UniRef50_A5DXY5	48.10%	501	2e-139	Uncharacterized protein n=2 Tax=Lodderomyces elongisporus (strain ATCC 11503 / CBS 2605 / JCM 1781 / NBRC 1676 / NRRL YB-4239) TaxID=379508 RepID=A5DXY5_LODEL
MCA_01665_1	39.11%	496	3e-99	MCA_01665_1
A0A161HJI4_9ASCO	29.49%	495	5e-58	Gag-pol fusion protein OS=Sugiyamaella lignohabitans GN=AWJ20_4398 PE=4 SV=1
YCH4_YEAST	38.38%	284	2e-60	Putative transposon Ty5-1 protein YCL074W OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=TY5A PE=5 SV=2
A0A1D8PLS1_CANAL	25.71%	560	2e-44	Uncharacterized protein OS=Candida albicans (strain SC5314 / ATCC MYA-2876) GN=orf19.2669 PE=4 SV=1

Mitochondrial localization by mitoprotII

Probability of mitochondrial location: 0.1924
Predicted cleavage: 15

Protein family membership

None predicted.

Domains and repeats

Domain
216 - 281
Zinc finger, CCHC-type (IPR001878)
649 - 700
GAG-pre-integrase domain (IPR025724)
709 - 912
Ribonuclease H-like domain (IPR012337)
Integrase, catalytic core (IPR001584)
1,285 - 1,527
Reverse transcriptase, RNA-dependent DNA polymerase (IPR013103)

1 200 400 600 800 1000 1200 1400 1600 1800 1800

Detailed signature matches

IPR001878

Zinc finger, CCHC-type
1. SM00343 (c2hcfinal6)
  
  240 - 256
  
  SMART
  SM00343 (c2hcfinal6)
2. G3DSA:4.10.60.10
  
  216 - 281
  
  GENE3D
  G3DSA:4.10.60.10 (G3DSA:4.10.60.10)
3. SSF57756 (Retroviru...)
  
  229 - 259
  
  SUPERFAMILY
  SSF57756 (Retrovirus zinc finger-like domains)
4. PS50158 (ZF_CCHC)
  
  241 - 255
  
  PROSITE profiles
  PS50158 (ZF_CCHC)
IPR025724

GAG-pre-integrase domain
1. PF13976 (gag_pre-in...)
  
  649 - 700
  
  Pfam
  PF13976 (gag_pre-integrs)
IPR012337

Ribonuclease H-like domain
1. SSF53098 (Ribonucle...)
  
  714 - 891
  
  SUPERFAMILY
  SSF53098 (Ribonuclease H-like)
2. G3DSA:3.30.42...
  
  709 - 912
  
  GENE3D
  G3DSA:3.30.420.10 (G3DSA:3.30.420.10)
IPR001584

Integrase, catalytic core
1. PF00665 (rve)
  
  714 - 832
  
  Pfam
  PF00665 (rve)
2. PS50994 (INTEGRASE)
  
  712 - 882
  
  PROSITE profiles
  PS50994 (INTEGRASE)
IPR013103

Reverse transcriptase, RNA-dependent DNA polymerase
1. PF07727 (RVT_2)
  
  1,285 - 1,527
  
  Pfam
  PF07727 (RVT_2)

no IPR

Unintegrated signatures

CYTOPLASMIC_D... (C...)

1 - 466

PHOBIUS
CYTOPLASMIC_DOMAIN (Cytoplasmic domain)
NON_CYTOPLASM... (N...)

490 - 1,800

PHOBIUS
NON_CYTOPLASMIC_DOMAIN (Non cytoplasmic domain)
SSF56672 (DNA/RNA p...)

1,537 - 1,719

SUPERFAMILY
SSF56672 (DNA/RNA polymerases)

1,284 - 1,507

SUPERFAMILY
SSF56672 (DNA/RNA polymerases)
TMhelix

467 - 489

TMHMM
TMhelix (TMhelix)
TRANSMEMBRANE (Tran...)

467 - 489

PHOBIUS
TRANSMEMBRANE (Transmembrane region)
cd09272 (RNase_HI_R...)

1,613 - 1,751

CDD
cd09272 (RNase_HI_RT_Ty1)
mobidb-lite (disord...)

248 - 280

MobiDB Lite
mobidb-lite (disorder_prediction)

993 - 1,127

MobiDB Lite
mobidb-lite (disorder_prediction)

Residue annotation

active site cd09272

1,617D

active site
1,617D, 1,660E, 1,694D, 1,743D
CDD
cd09272 (RNase_HI_RT_Ty1)

1,660E

active site
1,617D, 1,660E, 1,694D, 1,743D
CDD
cd09272 (RNase_HI_RT_Ty1)

1,694D

active site
1,617D, 1,660E, 1,694D, 1,743D
CDD
cd09272 (RNase_HI_RT_Ty1)

1,743D

active site
1,617D, 1,660E, 1,694D, 1,743D
CDD
cd09272 (RNase_HI_RT_Ty1)
RNA/DNA hybrid bin...

1,617D

RNA/DNA hybrid binding site
1,617D, 1,618A, 1,619D, 1,620Y, 1,655S, 1,656S, 1,657V, 1,660E, 1,694D, 1,720V, 1,747K
CDD
cd09272 (RNase_HI_RT_Ty1)

1,618A

RNA/DNA hybrid binding site
1,617D, 1,618A, 1,619D, 1,620Y, 1,655S, 1,656S, 1,657V, 1,660E, 1,694D, 1,720V, 1,747K
CDD
cd09272 (RNase_HI_RT_Ty1)

1,619D

RNA/DNA hybrid binding site
1,617D, 1,618A, 1,619D, 1,620Y, 1,655S, 1,656S, 1,657V, 1,660E, 1,694D, 1,720V, 1,747K
CDD
cd09272 (RNase_HI_RT_Ty1)

1,620Y

RNA/DNA hybrid binding site
1,617D, 1,618A, 1,619D, 1,620Y, 1,655S, 1,656S, 1,657V, 1,660E, 1,694D, 1,720V, 1,747K
CDD
cd09272 (RNase_HI_RT_Ty1)

1,655S

RNA/DNA hybrid binding site
1,617D, 1,618A, 1,619D, 1,620Y, 1,655S, 1,656S, 1,657V, 1,660E, 1,694D, 1,720V, 1,747K
CDD
cd09272 (RNase_HI_RT_Ty1)

1,656S

RNA/DNA hybrid binding site
1,617D, 1,618A, 1,619D, 1,620Y, 1,655S, 1,656S, 1,657V, 1,660E, 1,694D, 1,720V, 1,747K
CDD
cd09272 (RNase_HI_RT_Ty1)

1,657V

RNA/DNA hybrid binding site
1,617D, 1,618A, 1,619D, 1,620Y, 1,655S, 1,656S, 1,657V, 1,660E, 1,694D, 1,720V, 1,747K
CDD
cd09272 (RNase_HI_RT_Ty1)

1,660E

RNA/DNA hybrid binding site
1,617D, 1,618A, 1,619D, 1,620Y, 1,655S, 1,656S, 1,657V, 1,660E, 1,694D, 1,720V, 1,747K
CDD
cd09272 (RNase_HI_RT_Ty1)

1,694D

RNA/DNA hybrid binding site
1,617D, 1,618A, 1,619D, 1,620Y, 1,655S, 1,656S, 1,657V, 1,660E, 1,694D, 1,720V, 1,747K
CDD
cd09272 (RNase_HI_RT_Ty1)

1,720V

RNA/DNA hybrid binding site
1,617D, 1,618A, 1,619D, 1,620Y, 1,655S, 1,656S, 1,657V, 1,660E, 1,694D, 1,720V, 1,747K
CDD
cd09272 (RNase_HI_RT_Ty1)

1,747K

RNA/DNA hybrid binding site
1,617D, 1,618A, 1,619D, 1,620Y, 1,655S, 1,656S, 1,657V, 1,660E, 1,694D, 1,720V, 1,747K
CDD
cd09272 (RNase_HI_RT_Ty1)

Protein sequence

>MIA_04440_1
MTTRVTVQQVMGRTSAYINLTSSEKLNPLNYHAWATKILLGLRAMNNGVHLYVEAGTVSPGPNESLDDLTASLDTVVHDV
ILNNISPELIEQVNDVAIRGRDLWLYLHQEYSQLQPADVISLMKSLYAVNIESGPKLVSSIRTHVATWRSIYQSLSPDSV
VAFNILASMSPNCERLIQYALEHRFDGNNADNLDINNFVRNADKWAKLLKLTGSPATLSTEAFSASSKRHGNRSRNNVLT
CFRCKKRGHTSNRCPASWDEAQAAQQDKDKDDKESKDGKSSRAWFAEAVDGFTQIVDDDSFVSYAFESKTSDVCEKFGID
CSESDDDSESELFDSSEPYNGDEDYFDYQVSESCSNSELDVVPAADPFLGCTEDPCFVSEPCVGSGDDPCDPESFIGSPI
ISFVPEPLVGSDDGVFVEPFPSDVTSLADPVVDSCSFDIDSMADPIVDPSSSVTHPTDTKKSRLSPLLYLFVFAFLLLSL
ILLVLLHLFPFLDSRQSVHQAFVAESAASAEAHLVVGPDTFDFIHDTGATSHICNNISYFTNLRPTSTTIRGLGSSSAKA
VGDIVVDVLGQDGNPFDRVVLRDVLYVPNIPRNLFAARRAIAGGCQFIENLDHISAVENGTTRVHAIAMGDLYFCRFRVV
LPSKPVHEVFAIEPSANLWHQRLGHASVEVLKKLSKSLSVKPTHFEVVDSHKCDACLGGKATSLPFTGTTEKATRPLELL
VSDLSGPHVATPVGHRYVLTIMDFYSRYSYVELLVKKSDASLAIRNYIARCESYFSGDSRGNRVSYFHSDNGGEYISKDL
EQFFVSKGIKHTFTVPHTPQQNGVAERLNRTLFEKAKSMLLYSHAPDYLWGEAVKCANYLRNRLPSRTTGGVCPLQLWTG
KPPSYQHMRVFGCRANVVIADAKRESKLSSNIDPGVFVGYSLDRTAYRVLVDSSIVEARDVYFDESQFPFMKSDKELSSN
ASGIAFGIGCGSGSMSGPRADCNDSGTGSSVGLNSIAGSNSSSGSSFGSRRSLPDVTSVSGSGSSASSTSGSTGSSPSPS
LSSSPRSSSSLVVHKPRSVRRILPSSSAPLESPTLPSLPSSPILPTSDQDPSVSSPIGPSRSDLLQVGSPAGESDNSGDS
GISQRGVISGFTRIPPSVELEIVPLDQEPPSSDLVKSLDQAGVSVDTKDDTQAILPKSRSRVIAVRLPSVTPAKRPSSHD
FVAPPSKHRCDDSAKLAASSVVPAKRRAEQESISYRRLKSLRFEYDDVALLVFTDPTTFEQAMSSEDAEFWLEACVLEMS
SLNRNRTWDLVDLPPGRKAINSKWVFKIKRKADGSIERYKARLVCIGFSQVEGIDYTETFAPVVRYETVRIVLAIAAQFG
FQVHHMDVETAFLNGDLKEDIYMRQPKGFIVKGQESKVCHLNKSLYGLKQAPLCWNEKIHGALVKLGFVRNESDYGVYTK
GRGSAMVIIALYVDDLLIAGSSTHAIANTKSSLSSMFKMKDLGLVEQFLGMRVKQSPYHITVDVCRYIVDMLEEFGMQNC
SSVKTPLPTRDLSDFSESDAAADPSLYRSIIGKLIYAANCARPDLAVAVSFLCRYMQNPKSMHMEAAKHTLRYLKGSAQL
GLEYRAQKVYKLVGYSDADYAQDKQDRKSFTGYVFILSGGPITWACRKQVSPASSSVESEYMSLSDASKECFWINQLLSL
CQIPVPLPVTMFEDNQGCIALAQNPVFHRRTKHIDVRYHVVRHYIRSGVIHLEYLDTQVMLADMFTKNLGRVKFETLRGL
LGMKAVGDSATRGGVEHAMLSQTSACENCWEIMVDGLCLF

GO term prediction

Biological Process

GO:0015074 DNA integration

Molecular Function

GO:0003676 nucleic acid binding
GO:0008270 zinc ion binding

Cellular Component