Protein

MIA_03970_1

Length
299 amino acids

Browser: contig05:380017-381040+

Protein function

EGGNOG:0PFK1TRM8Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA (By similarity)
SGD closest match:S000002360TRM8tRNA (guanine-N(7)-)-methyltransferase
CGD closest match:CAL0000183898TRM8tRNA (guanine-N(7)-)-methyltransferase

Protein alignments

%idAln lengthE-value
MCA_02166_181.85%2703e-163MCA_02166_1
A0A060TDX9_BLAAD67.71%2884e-136tRNA (guanine-N(7)-)-methyltransferase OS=Blastobotrys adeninivorans GN=TRM8 PE=3 SV=1
A0A1E3PQA1_9ASCO63.93%3058e-136tRNA (guanine-N(7)-)-methyltransferase OS=Nadsonia fulvescens var. elongata DSM 6958 GN=TRM8 PE=3 SV=1
TRMB_YARLI69.49%2727e-134tRNA (guanine-N(7)-)-methyltransferase OS=Yarrowia lipolytica (strain CLIB 122 / E 150) GN=TRM8 PE=3 SV=1
UniRef50_Q6CE1269.49%2722e-130tRNA (guanine-N(7)-)-methyltransferase n=11 Tax=Dikarya TaxID=451864 RepID=TRMB_YARLI
A0A0J9XAM2_GEOCN63.97%2971e-133tRNA (guanine-N(7)-)-methyltransferase OS=Geotrichum candidum GN=TRM8 PE=3 SV=1
TRMB_YEAST69.37%2714e-130tRNA (guanine-N(7)-)-methyltransferase OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=TRM8 PE=1 SV=1
TRMB_CANAL65.99%2471e-118tRNA (guanine-N(7)-)-methyltransferase OS=Candida albicans (strain SC5314 / ATCC MYA-2876) GN=TRM8 PE=3 SV=3
A0A161HG11_9ASCO74.02%2049e-112tRNA (guanine-N(7)-)-methyltransferase OS=Sugiyamaella lignohabitans GN=TRM8 PE=3 SV=1
A0A1E4TJX0_9ASCO67.95%1563e-77tRNA (guanine(46)-N(7))-methyltransferase OS=Tortispora caseinolytica NRRL Y-17796 GN=CANCADRAFT_30330 PE=3 SV=1

Mitochondrial localization by mitoprotII

Probability of mitochondrial location: 0.2007

Protein family membership

Domains and repeats

  1. Domain
1 50 100 150 200 250 299

Detailed signature matches

  1. Family IPR003358
    tRNA (guanine-N-7) methyltransferase, Trmb type
    1. PS51625 (SAM_MT_TRMB)
    2. PF02390 (Methyltran...)
    3. TIGR00091
  2. Family IPR025763
    tRNA (guanine-N-7) methyltransferase catalytic subunit Trm8, eukaryote
    1. MF_03055 (tRNA_meth...)
  3. Domain IPR029063
    S-adenosyl-L-methionine-dependent methyltransferase
    1. SSF53335 (S-adenosy...)
Unintegrated signatures no IPR
Unintegrated signatures
  1. G3DSA:3.40.50...
  2. cd02440 (AdoMet_MTases)

Residue annotation

  1. S-adenosylmethioni...

Protein sequence

>MIA_03970_1
MTEPIEKQTEESVVAAVVDTPVEGEAHSEIIHKRKAYRLEQEAKRKVLKTVKFDEKDKDDEIKLPKKKYYRQRAHSNPFS
DHQLEYPKSPKDMKWDVLFPIIRDDPSKKVTIADIGCGFGGLLMSLSPAFPDKCILGMEIRVQVTQFVEDKIIALRKKHA
DENAYNNIAVLRGNAMKFLPNFFEKGQLEKIFFCFPDPHFKSKKHKARIVTTTLCSEYAYVLKPGGVVYTITDVKDLHEW
MVKHLDEHPLFERMSEDFEKNDTCAKIMYTDTEEGKKVARNEGDKFVACFKRLETPLEI

GO term prediction

Biological Process

GO:0006400 tRNA modification

Molecular Function

GO:0008176 tRNA (guanine-N7-)-methyltransferase activity

Cellular Component

None predicted.