Protein

MCA_02166_1

Length
301 amino acids

Gene name: TRM8

Description: tRNA (guanine-N(7)-)-methyltransferase

Browser: contigB:443634-444678-

RNA-seq: read pairs 931, FPKM 38.1, percentile rank 59.3% (100% = highest expression)

Protein function

Annotation:TRM8tRNA (guanine-N(7)-)-methyltransferase
KEGG:K03439trmB tRNA (guanine-N7-)-methyltransferase [EC:2.1.1.33]
EGGNOG:0PFK1TRM8Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA (By similarity)
SGD closest match:S000002360TRM8tRNA (guanine-N(7)-)-methyltransferase
CGD closest match:CAL0000183898TRM8tRNA (guanine-N(7)-)-methyltransferase

Protein alignments

%idAln lengthE-value
MIA_03970_184.07%2704e-176MIA_03970_1
A0A1E3PQA1_9ASCO69.05%2942e-153tRNA (guanine-N(7)-)-methyltransferase OS=Nadsonia fulvescens var. elongata DSM 6958 GN=TRM8 PE=3 SV=1
A0A060TDX9_BLAAD72.53%2731e-146tRNA (guanine-N(7)-)-methyltransferase OS=Blastobotrys adeninivorans GN=TRM8 PE=3 SV=1
TRMB_YARLI68.20%2831e-143tRNA (guanine-N(7)-)-methyltransferase OS=Yarrowia lipolytica (strain CLIB 122 / E 150) GN=TRM8 PE=3 SV=1
UniRef50_Q6CE1268.20%2833e-140tRNA (guanine-N(7)-)-methyltransferase n=11 Tax=Dikarya TaxID=451864 RepID=TRMB_YARLI
A0A0J9XAM2_GEOCN67.02%2825e-141tRNA (guanine-N(7)-)-methyltransferase OS=Geotrichum candidum GN=TRM8 PE=3 SV=1
TRMB_YEAST68.66%2682e-135tRNA (guanine-N(7)-)-methyltransferase OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=TRM8 PE=1 SV=1
TRMB_CANAL58.39%2984e-123tRNA (guanine-N(7)-)-methyltransferase OS=Candida albicans (strain SC5314 / ATCC MYA-2876) GN=TRM8 PE=3 SV=3
A0A161HG11_9ASCO75.00%2044e-115tRNA (guanine-N(7)-)-methyltransferase OS=Sugiyamaella lignohabitans GN=TRM8 PE=3 SV=1
A0A1E4TJX0_9ASCO65.38%1563e-74tRNA (guanine(46)-N(7))-methyltransferase OS=Tortispora caseinolytica NRRL Y-17796 GN=CANCADRAFT_30330 PE=3 SV=1

Mitochondrial localization by mitoprotII

Probability of mitochondrial location: 0.0339

Protein family membership

Domains and repeats

  1. Domain
1 50 100 150 200 250 301

Detailed signature matches

  1. Family IPR003358
    tRNA (guanine-N-7) methyltransferase, Trmb type
    1. PS51625 (SAM_MT_TRMB)
    2. PF02390 (Methyltran...)
    3. TIGR00091
  2. Family IPR025763
    tRNA (guanine-N-7) methyltransferase catalytic subunit Trm8, eukaryote
    1. MF_03055 (tRNA_meth...)
  3. Domain IPR029063
    S-adenosyl-L-methionine-dependent methyltransferase
    1. SSF53335 (S-adenosy...)
Unintegrated signatures no IPR
Unintegrated signatures
  1. G3DSA:3.40.50...
  2. cd02440 (AdoMet_MTases)
  3. mobidb-lite (disord...)

Residue annotation

  1. S-adenosylmethioni...

Protein sequence

>MCA_02166_1
MSEKKEIENSDLSKSLDISSNQETVEKSTTESQNEVMHKRKAYRIQQEEKRKALKTQKFEEKDDTVKLPKKKFYRQRAHS
NPFSDHQLEYPKSPKDMDWATLFPAIRDDPSKKVTIADIGCGFGGLLMALSPQFPDKCILGMEIRVQVTQFVEDKIIALR
KKHADSNGYNNISVIRGNAMKFLPNFFEKGQLEKIFFCFPDPHFKSKKHKARIVTTTLCSEYAYVLKPGGIVYTITDVKD
LHDWMVEHLESHPLFERMPKEFEENDICAKTMYVETEEGKKVARNKGSKYVACFKRLDISK

GO term prediction

Biological Process

GO:0006400 tRNA modification

Molecular Function

GO:0008176 tRNA (guanine-N7-)-methyltransferase activity

Cellular Component

None predicted.