Protein

MIA_03882_1

Length
692 amino acids

Browser: contig05:125905-128031-

Protein function

EGGNOG:0PFW8KU70Single stranded DNA-dependent ATP-dependent helicase. Involved in non-homologous end joining (NHEJ) DNA double strand break repair. DNA-binding is sequence-independent but has a high affinity to nicks in double stranded DNA and to the ends of duplex DNA. Binds to naturally occurring chromosomal ends, and therefore provides chromosomal end protection. Required also for telomere recombination to repair telomeric ends in the absence of telomerase. ku70, of the ku70 ku80 heterodimer, binds to the stem loop of tlc1, the RNA component of telomerase. Involved in telomere maintenance. Interacts with telomeric repeats and subtelomeric sequences thereby controlling telomere length and protecting against subtelomeric rearrangement. Maintains telomeric chromatin, which is involved in silencing the expression of genes located at the telomere. Required for mating-type switching (By similarity)
SGD closest match:S000004897YKU70ATP-dependent DNA helicase II subunit 1
CGD closest match:CAL0000186914CAS1ATP-dependent DNA helicase

Protein alignments

%idAln lengthE-value
MCA_05664_128.878%7935.04e-81MCA_05664_1
A0A0J9XI50_GEOCN30.036%5563.98e-61Similar to Saccharomyces cerevisiae YMR284W YKU70 Subunit of the telomeric Ku complex (Yku70p-Yku80p) OS=Geotrichum candidum GN=BN980_GECA19s02155g PE=4 SV=1
UniRef50_A0A0J9XI5030.036%5568.14e-58Similar to Saccharomyces cerevisiae YMR284W YKU70 Subunit of the telomeric Ku complex (Yku70p-Yku80p) n=1 Tax=Geotrichum candidum TaxID=1173061 RepID=A0A0J9XI50_GEOCN
A0A1E3PT04_9ASCO29.636%5501.98e-53Ku DNA-binding complex, Ku70 subunit OS=Nadsonia fulvescens var. elongata DSM 6958 GN=NADFUDRAFT_63968 PE=4 SV=1
A0A060TBQ9_BLAAD27.094%5618.67e-47ARAD1D36938p OS=Blastobotrys adeninivorans GN=GNLVRS02_ARAD1D36938g PE=4 SV=1
KU70_YARLI27.512%4185.32e-41ATP-dependent DNA helicase II subunit 1 OS=Yarrowia lipolytica (strain CLIB 122 / E 150) GN=KU70 PE=3 SV=1
A0A1E4TM08_9ASCO28.313%4982.84e-40Uncharacterized protein OS=Tortispora caseinolytica NRRL Y-17796 GN=CANCADRAFT_1273 PE=4 SV=1
A0A167C337_9ASCO30.721%3193.32e-32Yku70p OS=Sugiyamaella lignohabitans GN=YKU70 PE=4 SV=1
KU70_YEAST22.705%4142.29e-17ATP-dependent DNA helicase II subunit 1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=YKU70 PE=1 SV=2
A0A1D8PF81_CANAL28.283%1981.86e-15ATP-dependent DNA helicase OS=Candida albicans (strain SC5314 / ATCC MYA-2876) GN=CAS1 PE=4 SV=1

Mitochondrial localization by mitoprotII

Probability of mitochondrial location: 0.1593

Protein family membership

Domains and repeats

  1. Domain
  2. Domain
1 100 200 300 400 500 600 692

Detailed signature matches

  1. Family IPR006165
    Ku70
    1. PIRSF003033 (Ku70)
  2. Domain IPR002035
    von Willebrand factor, type A
    1. SSF53300 (vWA-like)
    2. G3DSA:3.40.50...
  3. Domain IPR005161
    Ku70/Ku80, N-terminal alpha/beta
    1. PF03731 (Ku_N)
  4. Domain IPR016194
    SPOC-like, C-terminal domain
    1. SSF100939 (SPOC dom...)
    2. G3DSA:2.40.29...
  5. Domain IPR006164
    Ku70/Ku80 beta-barrel domain
    1. PF02735 (Ku)
    2. SM00559 (ku_4)
  6. Domain IPR005160
    Ku70/Ku80 C-terminal arm
    1. PF03730 (Ku_C)
Unintegrated signatures no IPR
Unintegrated signatures
  1. G3DSA:1.10.16...
  2. cd00788 (KU70)
  3. mobidb-lite (disord...)

Residue annotation

  1. DNA binding site c...
  2. heterodimer interf...

Protein sequence

>MIA_03882_1
MAPKDPWDDFAPATDETDFLNDADYAPEKDALILIIESTPSFYAPGPSPTQPHALMLLLAQIHAALFKRTLYDKSSLAAV
VLFNTNAPSPTVLIDLAVPSIADLQTFNLLATSRAHFEQSFAPAPPQASSSNSLLAALDAAASMLASKKLASYSSRRILL
FTDRDSPLHAPQIKLAAKTRLRDLAAAGAAFIPCFIHPRPESPFDTRLFYEDILLAAASSDQDSPLPAAPCSLAEARLAP
ALLALQSPPKRSLFHLPLHLSPSLSFGLQAYALISPQSVPRPVDVLAVTDKTTHTIERIPANRKRIAISTETGKTLAPEN
VVRAYKFSDVFVPFTRDQMAAMRNFGPPQLRLLGFKDAAAAGWEYSLEKSYFLYPSESHYGGSKRTYAALHRSLTQKKKV
ALVWAILRANAPPRIAVIVPHGPTVSSTSSDDSKQAAMHPSFPTGLHLVPLAYADDLRHLPPSITNPPSRPAPPSAHLVA
SAKTVIQALRMSIGYSPRRYPNPHLARFYHTLQQAVFEQERDPALSPDQSLPKYNSIYKRARSPILAWTAELRQLEYQPA
QASTYASRVTAKRAHDGGGGGDTAPAPKRAAPRNDYRATDDTSALARGGRRPRNAGATTETAALGRAQQHVLFERLRHQV
RAYPLGARFEDVTAAQLREFADEAGIATRASTKAGLRSAVSRYFDEHFGTGA

GO term prediction

Biological Process

GO:0000723 telomere maintenance
GO:0006303 double-strand break repair via nonhomologous end joining

Molecular Function

GO:0003677 DNA binding
GO:0003684 damaged DNA binding
GO:0004003 ATP-dependent DNA helicase activity
GO:0042162 telomeric DNA binding

Cellular Component

GO:0005634 nucleus
GO:0043564 Ku70:Ku80 complex