MIA_03869_1

Protein

Length

1,392 amino acids

Protein function

EGGNOG:	0PHVU	PGUG_01875	phosphoribosylformylglycinamidine synthase
SGD closest match:	S000003293	ADE6	Phosphoribosylformylglycinamidine synthase
CGD closest match:	CAL0000175495	ADE6	Phosphoribosylformylglycinamidine synthase

Protein alignments

	%id	Aln length
MCA_03194_1	77.928%	1409	MCA_03194_1
A0A0J9XCW9_GEOCN	73.994%	1392	Similar to Saccharomyces cerevisiae YGR061C ADE6 Formylglycinamidine-ribonucleotide (FGAM)-synthetase,catalyzes a step in the 'de novo' purine nucleotide biosynthetic pathway OS=Geotrichum candidum GN=BN980_GECA08s02496g PE=3 SV=1
A0A167E6F4_9ASCO	67.983%	1393	Phosphoribosylformylglycinamidine synthase OS=Sugiyamaella lignohabitans GN=ADE6 PE=3 SV=1
A0A060T9I1_BLAAD	65.161%	1395	ARAD1C44902p OS=Blastobotrys adeninivorans GN=GNLVRS02_ARAD1C44902g PE=3 SV=1
Q6BZY1_YARLI	64.685%	1396	YALI0F30019p OS=Yarrowia lipolytica (strain CLIB 122 / E 150) GN=YALI0_F30019g PE=3 SV=1
A0A1E3PM19_9ASCO	65.214%	1400	Phosphoribosylformylglycinamidin OS=Nadsonia fulvescens var. elongata DSM 6958 GN=NADFUDRAFT_51253 PE=3 SV=1
UniRef50_K0KCX6	62.376%	1414	Phosphoribosylformylglycinamidine synthase n=45 Tax=saccharomyceta TaxID=716545 RepID=K0KCX6_WICCF
PUR4_YEAST	58.392%	1430	Phosphoribosylformylglycinamidine synthase OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=ADE6 PE=1 SV=2
A0A1E4TKF4_9ASCO	58.720%	1422	Uncharacterized protein OS=Tortispora caseinolytica NRRL Y-17796 GN=CANCADRAFT_30462 PE=3 SV=1
Q59MZ5_CANAL	56.159%	1437	Phosphoribosylformylglycinamidine synthase OS=Candida albicans (strain SC5314 / ATCC MYA-2876) GN=ADE6 PE=3 SV=1

Mitochondrial localization by mitoprotII

Probability of mitochondrial location: 0.3882
Predicted cleavage: 18

Protein family membership

Phosphoribosylformylglycinamidine synthase (IPR010073)

Domains and repeats

Domain
270 - 299
PurM-like, N-terminal domain (IPR016188)
300 - 507
PurM-like, N-terminal domain (IPR016188)
725 - 904
PurM-like, N-terminal domain (IPR016188)
1,127 - 1,392
Class I glutamine amidotransferase-like (IPR029062)
Glutamine amidotransferase (IPR017926)
Domain
509 - 747
AIR synthase-related protein, C-terminal domain (IPR010918)
909 - 1,121
AIR synthase-related protein, C-terminal domain (IPR010918)

1 200 400 600 800 1000 1200 1392

Detailed signature matches

IPR010073

Phosphoribosylformylglycinamidine synthase
1. MF_00419 (PurL_1)
  
  3 - 1,392
  
  HAMAP
  MF_00419 (PurL_1)
2. TIGR01735
  
  153 - 1,391
  
  TIGRFAMs
  TIGR01735 (TIGR01735)
IPR016188

PurM-like, N-terminal domain
1. SSF55326 (PurM N-te...)
  
  303 - 506
  
  SUPERFAMILY
  SSF55326 (PurM N-terminal domain-like)
  
  725 - 904
  
  SUPERFAMILY
  SSF55326 (PurM N-terminal domain-like)
2. G3DSA:3.30.13...
  
  300 - 507
  
  GENE3D
  G3DSA:3.30.1330.10 (G3DSA:3.30.1330.10)
  
  270 - 299
  
  GENE3D
  G3DSA:3.30.1330.10 (G3DSA:3.30.1330.10)
  
  736 - 901
  
  GENE3D
  G3DSA:3.30.1330.10 (G3DSA:3.30.1330.10)
IPR010918

AIR synthase-related protein, C-terminal domain
1. PF02769 (AIRS_C)
  
  516 - 673
  
  Pfam
  PF02769 (AIRS_C)
  
  921 - 1,055
  
  Pfam
  PF02769 (AIRS_C)
2. SSF56042 (PurM C-te...)
  
  912 - 1,121
  
  SUPERFAMILY
  SSF56042 (PurM C-terminal domain-like)
  
  514 - 747
  
  SUPERFAMILY
  SSF56042 (PurM C-terminal domain-like)
3. G3DSA:3.90.65...
  
  909 - 1,085
  
  GENE3D
  G3DSA:3.90.650.10 (G3DSA:3.90.650.10)
  
  509 - 691
  
  GENE3D
  G3DSA:3.90.650.10 (G3DSA:3.90.650.10)
IPR029062

Class I glutamine amidotransferase-like
1. G3DSA:3.40.50...
  
  1,127 - 1,392
  
  GENE3D
  G3DSA:3.40.50.880 (G3DSA:3.40.50.880)
2. SSF52317 (Class I g...)
  
  1,127 - 1,391
  
  SUPERFAMILY
  SSF52317 (Class I glutamine amidotransferase-like)
IPR017926

Glutamine amidotransferase
1. PS51273 (GATASE_TYPE_1)
  
  1,131 - 1,392
  
  PROSITE profiles
  PS51273 (GATASE_TYPE_1)

no IPR

Unintegrated signatures

PF13507 (GATase_5)

1,128 - 1,391

Pfam
PF13507 (GATase_5)
SM01211 (GATase_5_2)

1,128 - 1,391

SMART
SM01211 (GATase_5_2)
SSF109736 (FGAM syn...)

246 - 302

SUPERFAMILY
SSF109736 (FGAM synthase PurL, linker domain)
SSF82697 (PurS-like)

149 - 241

SUPERFAMILY
SSF82697 (PurS-like)
cd01740 (GATase1_FG...)

1,131 - 1,388

CDD
cd01740 (GATase1_FGAR_AT)
cd02203 (PurL_repeat1)

287 - 684

CDD
cd02203 (PurL_repeat1)
cd02204 (PurL_repeat2)

771 - 1,044

CDD
cd02204 (PurL_repeat2)
mobidb-lite (disord...)

382 - 410

MobiDB Lite
mobidb-lite (disorder_prediction)

111 - 149

MobiDB Lite
mobidb-lite (disorder_prediction)

Residue annotation

dimerization inter...

370H

dimerization interface
370H, 371F, 372L, 373A, 375V, 400D, 412A, 415A, 418A, 470Y, 493P, 494V, 498G
CDD
cd02203 (PurL_repeat1)

371F

dimerization interface
370H, 371F, 372L, 373A, 375V, 400D, 412A, 415A, 418A, 470Y, 493P, 494V, 498G
CDD
cd02203 (PurL_repeat1)

372L

dimerization interface
370H, 371F, 372L, 373A, 375V, 400D, 412A, 415A, 418A, 470Y, 493P, 494V, 498G
CDD
cd02203 (PurL_repeat1)

373A

dimerization interface
370H, 371F, 372L, 373A, 375V, 400D, 412A, 415A, 418A, 470Y, 493P, 494V, 498G
CDD
cd02203 (PurL_repeat1)

375V

dimerization interface
370H, 371F, 372L, 373A, 375V, 400D, 412A, 415A, 418A, 470Y, 493P, 494V, 498G
CDD
cd02203 (PurL_repeat1)

400D

dimerization interface
370H, 371F, 372L, 373A, 375V, 400D, 412A, 415A, 418A, 470Y, 493P, 494V, 498G
CDD
cd02203 (PurL_repeat1)

412A

dimerization interface
370H, 371F, 372L, 373A, 375V, 400D, 412A, 415A, 418A, 470Y, 493P, 494V, 498G
CDD
cd02203 (PurL_repeat1)

415A

dimerization interface
370H, 371F, 372L, 373A, 375V, 400D, 412A, 415A, 418A, 470Y, 493P, 494V, 498G
CDD
cd02203 (PurL_repeat1)

418A

dimerization interface
370H, 371F, 372L, 373A, 375V, 400D, 412A, 415A, 418A, 470Y, 493P, 494V, 498G
CDD
cd02203 (PurL_repeat1)

470Y

dimerization interface
370H, 371F, 372L, 373A, 375V, 400D, 412A, 415A, 418A, 470Y, 493P, 494V, 498G
CDD
cd02203 (PurL_repeat1)

493P

dimerization interface
370H, 371F, 372L, 373A, 375V, 400D, 412A, 415A, 418A, 470Y, 493P, 494V, 498G
CDD
cd02203 (PurL_repeat1)

494V

dimerization interface
370H, 371F, 372L, 373A, 375V, 400D, 412A, 415A, 418A, 470Y, 493P, 494V, 498G
CDD
cd02203 (PurL_repeat1)

498G

dimerization interface
370H, 371F, 372L, 373A, 375V, 400D, 412A, 415A, 418A, 470Y, 493P, 494V, 498G
CDD
cd02203 (PurL_repeat1)
ATP binding site c...

396G

ATP binding site
396G, 400D, 468A, 469G, 470Y
CDD
cd02203 (PurL_repeat1)

400D

ATP binding site
396G, 400D, 468A, 469G, 470Y
CDD
cd02203 (PurL_repeat1)

468A

ATP binding site
396G, 400D, 468A, 469G, 470Y
CDD
cd02203 (PurL_repeat1)

469G

ATP binding site
396G, 400D, 468A, 469G, 470Y
CDD
cd02203 (PurL_repeat1)

470Y

ATP binding site
396G, 400D, 468A, 469G, 470Y
CDD
cd02203 (PurL_repeat1)
dimerization inter...

784G

dimerization interface
784G, 785E, 786S, 787F, 789C, 814N, 827R, 830A, 833M, 868K, 886L, 887T, 891T
CDD
cd02204 (PurL_repeat2)

785E

dimerization interface
784G, 785E, 786S, 787F, 789C, 814N, 827R, 830A, 833M, 868K, 886L, 887T, 891T
CDD
cd02204 (PurL_repeat2)

786S

dimerization interface
784G, 785E, 786S, 787F, 789C, 814N, 827R, 830A, 833M, 868K, 886L, 887T, 891T
CDD
cd02204 (PurL_repeat2)

787F

dimerization interface
784G, 785E, 786S, 787F, 789C, 814N, 827R, 830A, 833M, 868K, 886L, 887T, 891T
CDD
cd02204 (PurL_repeat2)

789C

dimerization interface
784G, 785E, 786S, 787F, 789C, 814N, 827R, 830A, 833M, 868K, 886L, 887T, 891T
CDD
cd02204 (PurL_repeat2)

814N

dimerization interface
784G, 785E, 786S, 787F, 789C, 814N, 827R, 830A, 833M, 868K, 886L, 887T, 891T
CDD
cd02204 (PurL_repeat2)

827R

dimerization interface
784G, 785E, 786S, 787F, 789C, 814N, 827R, 830A, 833M, 868K, 886L, 887T, 891T
CDD
cd02204 (PurL_repeat2)

830A

dimerization interface
784G, 785E, 786S, 787F, 789C, 814N, 827R, 830A, 833M, 868K, 886L, 887T, 891T
CDD
cd02204 (PurL_repeat2)

833M

dimerization interface
784G, 785E, 786S, 787F, 789C, 814N, 827R, 830A, 833M, 868K, 886L, 887T, 891T
CDD
cd02204 (PurL_repeat2)

868K

dimerization interface
784G, 785E, 786S, 787F, 789C, 814N, 827R, 830A, 833M, 868K, 886L, 887T, 891T
CDD
cd02204 (PurL_repeat2)

886L

dimerization interface
784G, 785E, 786S, 787F, 789C, 814N, 827R, 830A, 833M, 868K, 886L, 887T, 891T
CDD
cd02204 (PurL_repeat2)

887T

dimerization interface
784G, 785E, 786S, 787F, 789C, 814N, 827R, 830A, 833M, 868K, 886L, 887T, 891T
CDD
cd02204 (PurL_repeat2)

891T

dimerization interface
784G, 785E, 786S, 787F, 789C, 814N, 827R, 830A, 833M, 868K, 886L, 887T, 891T
CDD
cd02204 (PurL_repeat2)
ATP binding site c...

810E

ATP binding site
810E, 814N, 866V, 867G, 868K
CDD
cd02204 (PurL_repeat2)

814N

ATP binding site
810E, 814N, 866V, 867G, 868K
CDD
cd02204 (PurL_repeat2)

866V

ATP binding site
810E, 814N, 866V, 867G, 868K
CDD
cd02204 (PurL_repeat2)

867G

ATP binding site
810E, 814N, 866V, 867G, 868K
CDD
cd02204 (PurL_repeat2)

868K

ATP binding site
810E, 814N, 866V, 867G, 868K
CDD
cd02204 (PurL_repeat2)
putative active si...

1,182G

putative active site
1,182G, 1,183F, 1,187D, 1,225C, 1,229Q, 1,293A, 1,294H, 1,356M, 1,358H, 1,359P, 1,360E, 1,361R
CDD
cd01740 (GATase1_FGAR_AT)

1,183F

putative active site
1,182G, 1,183F, 1,187D, 1,225C, 1,229Q, 1,293A, 1,294H, 1,356M, 1,358H, 1,359P, 1,360E, 1,361R
CDD
cd01740 (GATase1_FGAR_AT)

1,187D

putative active site
1,182G, 1,183F, 1,187D, 1,225C, 1,229Q, 1,293A, 1,294H, 1,356M, 1,358H, 1,359P, 1,360E, 1,361R
CDD
cd01740 (GATase1_FGAR_AT)

1,225C

putative active site
1,182G, 1,183F, 1,187D, 1,225C, 1,229Q, 1,293A, 1,294H, 1,356M, 1,358H, 1,359P, 1,360E, 1,361R
CDD
cd01740 (GATase1_FGAR_AT)

1,229Q

putative active site
1,182G, 1,183F, 1,187D, 1,225C, 1,229Q, 1,293A, 1,294H, 1,356M, 1,358H, 1,359P, 1,360E, 1,361R
CDD
cd01740 (GATase1_FGAR_AT)

1,293A

putative active site
1,182G, 1,183F, 1,187D, 1,225C, 1,229Q, 1,293A, 1,294H, 1,356M, 1,358H, 1,359P, 1,360E, 1,361R
CDD
cd01740 (GATase1_FGAR_AT)

1,294H

putative active site
1,182G, 1,183F, 1,187D, 1,225C, 1,229Q, 1,293A, 1,294H, 1,356M, 1,358H, 1,359P, 1,360E, 1,361R
CDD
cd01740 (GATase1_FGAR_AT)

1,356M

putative active site
1,182G, 1,183F, 1,187D, 1,225C, 1,229Q, 1,293A, 1,294H, 1,356M, 1,358H, 1,359P, 1,360E, 1,361R
CDD
cd01740 (GATase1_FGAR_AT)

1,358H

putative active site
1,182G, 1,183F, 1,187D, 1,225C, 1,229Q, 1,293A, 1,294H, 1,356M, 1,358H, 1,359P, 1,360E, 1,361R
CDD
cd01740 (GATase1_FGAR_AT)

1,359P

putative active site
1,182G, 1,183F, 1,187D, 1,225C, 1,229Q, 1,293A, 1,294H, 1,356M, 1,358H, 1,359P, 1,360E, 1,361R
CDD
cd01740 (GATase1_FGAR_AT)

1,360E

putative active site
1,182G, 1,183F, 1,187D, 1,225C, 1,229Q, 1,293A, 1,294H, 1,356M, 1,358H, 1,359P, 1,360E, 1,361R
CDD
cd01740 (GATase1_FGAR_AT)

1,361R

putative active site
1,182G, 1,183F, 1,187D, 1,225C, 1,229Q, 1,293A, 1,294H, 1,356M, 1,358H, 1,359P, 1,360E, 1,361R
CDD
cd01740 (GATase1_FGAR_AT)
catalytic triad cd...

1,225C

catalytic triad
1,225C, 1,358H, 1,360E
CDD
cd01740 (GATase1_FGAR_AT)

1,358H

catalytic triad
1,225C, 1,358H, 1,360E
CDD
cd01740 (GATase1_FGAR_AT)

1,360E

catalytic triad
1,225C, 1,358H, 1,360E
CDD
cd01740 (GATase1_FGAR_AT)

Protein sequence

>MIA_03869_1
MTLIALPGPVAYSPFRISSLINHINTSLNSSAVVAIRSVYVHYADIAPSSSILAPASGPLDDANDDAAKDKPISKLALLK
ALLEYDSKPDPSDPLTQALLDSITDSASAQTAAAVSSATAPSGPPTAPALDQPSTSTDSLESSSTSSIASPLPENTYLIR
VIPRPGTVSPWSSKATNIAQVCGLGDDVLRIERGIDFIFQVRKSFPFEEYLKSGVFLDYVYDRMTQYIPSGTPSSEDLFA
HHKPKPLVHVPRNGIPEANKTLGLALDQAEIDYLVDSFPAREPTDVELFMFAQVNSEHCRHKIFNADWIIDGEAKPYTLF
KMIRNTHNTNPQYTISAYSDNSAVLQGAKSYFFAPDPVSHVYTKDPETQHFLAKVETHNHPTAVSPFPGAATGSGGEIRD
EGATGRGSRPKAGLAGYAVSDLLIPDFKQPWELDVGKPAHISSALDIMLQAPIGAASFNNEFGRPNIAGYFRTLTTTVTN
AAGKQEIRGFHKPVMVAGGLGTVRPQFALKDRGINPGSAIIVLGGPAMLIGLGGGAASSVASGDAAAHLDFASVQRGNPE
MQRRAQMVIDACVALGPANPIQSIHDVGAGGLSNALTELVHDNDLGAKFELRDVPSIEPGMSPMEIWCNEAQERYVLAVA
PNDLEAFETICKRERAIYGVVGHATSEKRLILTDRLLGSTPIDIEMSVLFGKPPRMIRNTTTKPLKLSPFDQSLAAYSVS
PVDAISTALERVLSLPSVGSKSFLITIGDRSVTGLITRDQMVGPWQTPVSDAAVTLSSFGVSHGESFACGERPSLALINP
GASAKVAVAESLLNLYASDVQDLQRVRLSANWMSAASHEGEGAALYEAVQAIGLDLCPALGVSIPVGKDSMSMKMKWNNR
EVTAPLTCNITAFAPVDDVARTWTPQLQRVQDSKLVLVDLGLGQNRLGGSALAQVFGQVGDEAPNVDDNSLLRTFLKTLE
KLHKSTNLVQAYHDRSDGGLITTLLEMAFAGRVGLDINLEASFGDAISQLFNEELGAVFQVTDADEFIAFLTEEGIPPQA
ISVVAQVNTQKEQYINISYGGSRIYSNNRATLQQVWSSTSHRLQRLRDNPKSADQEFSNILDNSDPGLSFKLTYDPTENI
VANLLPQKPRVAILREQGVNGHNEMAYAMMLAGFTPVDVHMSDLAAGRFDLADFVGLAACGGFSYGDVLGAGAGWAKSVL
YNAELRAMFKHFFNDRSDTFALGVCNGCQFLSRLSSIIPGAENWPLFERNASEQYEARTVMVEVVDDATNSAQDQPSIFF
SGMRGSRFPVAVAHGEGRASFESSEAAKQFAKDNLTVLRFVDNYGKTTERYPYNPNGSPEGVTGIRTPNGRVLAMMPHPE
RVTVLETNSWYPDNVAEGPWLRLFRNARKWVA

GO term prediction

Biological Process

GO:0006189 'de novo' IMP biosynthetic process

Molecular Function

GO:0004642 phosphoribosylformylglycinamidine synthase activity

Cellular Component

None predicted.

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Entry type

Status

Per-residue features