MCA_03194_1

Protein

Length

1,351 amino acids

Gene name: ADE6

Description: Phosphoribosylformylglycinamidine synthase

Browser: contigB:3614654-3618853-

RNA-seq: read pairs 8414, FPKM 76.9, percentile rank 74.6% (100% = highest expression)

Protein function

Annotation:		ADE6	Phosphoribosylformylglycinamidine synthase
KEGG:	K01952	purL	phosphoribosylformylglycinamidine synthase [EC:6.3.5.3]
EGGNOG:	0PHVU	PGUG_01875	phosphoribosylformylglycinamidine synthase
SGD closest match:	S000003293	ADE6	Phosphoribosylformylglycinamidine synthase
CGD closest match:	CAL0000175495	ADE6	Phosphoribosylformylglycinamidine synthase

Protein alignments

	%id	Aln length
MIA_03869_1	78.12%	1408	MIA_03869_1
A0A0J9XCW9_GEOCN	77.33%	1350	Similar to Saccharomyces cerevisiae YGR061C ADE6 Formylglycinamidine-ribonucleotide (FGAM)-synthetase,catalyzes a step in the 'de novo' purine nucleotide biosynthetic pathway OS=Geotrichum candidum GN=BN980_GECA08s02496g PE=3 SV=1
A0A167E6F4_9ASCO	71.06%	1351	Phosphoribosylformylglycinamidine synthase OS=Sugiyamaella lignohabitans GN=ADE6 PE=3 SV=1
A0A060T9I1_BLAAD	68.88%	1353	ARAD1C44902p OS=Blastobotrys adeninivorans GN=GNLVRS02_ARAD1C44902g PE=3 SV=1
Q6BZY1_YARLI	68.14%	1356	YALI0F30019p OS=Yarrowia lipolytica (strain CLIB 122 / E 150) GN=YALI0_F30019g PE=3 SV=1
A0A1E3PM19_9ASCO	68.11%	1358	Phosphoribosylformylglycinamidin OS=Nadsonia fulvescens var. elongata DSM 6958 GN=NADFUDRAFT_51253 PE=3 SV=1
UniRef50_K0KCX6	63.85%	1375	Phosphoribosylformylglycinamidine synthase n=45 Tax=saccharomyceta TaxID=716545 RepID=K0KCX6_WICCF
PUR4_YEAST	61.29%	1390	Phosphoribosylformylglycinamidine synthase OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=ADE6 PE=1 SV=2
A0A1E4TKF4_9ASCO	60.29%	1385	Uncharacterized protein OS=Tortispora caseinolytica NRRL Y-17796 GN=CANCADRAFT_30462 PE=3 SV=1
Q59MZ5_CANAL	58.11%	1399	Phosphoribosylformylglycinamidine synthase OS=Candida albicans (strain SC5314 / ATCC MYA-2876) GN=ADE6 PE=3 SV=1

Mitochondrial localization by mitoprotII

Probability of mitochondrial location: 0.8853
Predicted cleavage: 18

Protein family membership

Phosphoribosylformylglycinamidine synthase (IPR010073)

Domains and repeats

Domain
211 - 242
PurM-like, N-terminal domain (IPR016188)
243 - 450
PurM-like, N-terminal domain (IPR016188)
452 - 641
AIR synthase-related protein, C-terminal domain (IPR010918)
668 - 847
PurM-like, N-terminal domain (IPR016188)
855 - 1,063
AIR synthase-related protein, C-terminal domain (IPR010918)
1,068 - 1,351
Class I glutamine amidotransferase-like (IPR029062)
Glutamine amidotransferase (IPR017926)

1 200 400 600 800 1000 1200 1351

Detailed signature matches

IPR010073

Phosphoribosylformylglycinamidine synthase
1. MF_00419 (PurL_1)
  
  3 - 1,351
  
  HAMAP
  MF_00419 (PurL_1)
2. TIGR01735
  
  7 - 1,350
  
  TIGRFAMs
  TIGR01735 (TIGR01735)
IPR016188

PurM-like, N-terminal domain
1. SSF55326 (PurM N-te...)
  
  668 - 847
  
  SUPERFAMILY
  SSF55326 (PurM N-terminal domain-like)
  
  246 - 449
  
  SUPERFAMILY
  SSF55326 (PurM N-terminal domain-like)
2. G3DSA:3.30.13...
  
  211 - 242
  
  GENE3D
  G3DSA:3.30.1330.10 (G3DSA:3.30.1330.10)
  
  243 - 450
  
  GENE3D
  G3DSA:3.30.1330.10 (G3DSA:3.30.1330.10)
  
  679 - 844
  
  GENE3D
  G3DSA:3.30.1330.10 (G3DSA:3.30.1330.10)
IPR010918

AIR synthase-related protein, C-terminal domain
1. PF02769 (AIRS_C)
  
  864 - 994
  
  Pfam
  PF02769 (AIRS_C)
  
  459 - 616
  
  Pfam
  PF02769 (AIRS_C)
2. SSF56042 (PurM C-te...)
  
  855 - 1,063
  
  SUPERFAMILY
  SSF56042 (PurM C-terminal domain-like)
  
  458 - 641
  
  SUPERFAMILY
  SSF56042 (PurM C-terminal domain-like)
3. G3DSA:3.90.65...
  
  855 - 1,027
  
  GENE3D
  G3DSA:3.90.650.10 (G3DSA:3.90.650.10)
  
  452 - 634
  
  GENE3D
  G3DSA:3.90.650.10 (G3DSA:3.90.650.10)
IPR029062

Class I glutamine amidotransferase-like
1. G3DSA:3.40.50...
  
  1,069 - 1,350
  
  GENE3D
  G3DSA:3.40.50.880 (G3DSA:3.40.50.880)
2. SSF52317 (Class I g...)
  
  1,068 - 1,350
  
  SUPERFAMILY
  SSF52317 (Class I glutamine amidotransferase-like)
IPR017926

Glutamine amidotransferase
1. PS51273 (GATASE_TYPE_1)
  
  1,073 - 1,351
  
  PROSITE profiles
  PS51273 (GATASE_TYPE_1)

no IPR

Unintegrated signatures

PF13507 (GATase_5)

1,070 - 1,350

Pfam
PF13507 (GATase_5)
SM01211 (GATase_5_2)

1,070 - 1,350

SMART
SM01211 (GATase_5_2)
SSF109736 (FGAM syn...)

189 - 245

SUPERFAMILY
SSF109736 (FGAM synthase PurL, linker domain)
SSF82697 (PurS-like)

4 - 182

SUPERFAMILY
SSF82697 (PurS-like)
cd01740 (GATase1_FG...)

1,073 - 1,347

CDD
cd01740 (GATase1_FGAR_AT)
cd02203 (PurL_repeat1)

230 - 627

CDD
cd02203 (PurL_repeat1)
cd02204 (PurL_repeat2)

727 - 988

CDD
cd02204 (PurL_repeat2)
mobidb-lite (disord...)

1,213 - 1,232

MobiDB Lite
mobidb-lite (disorder_prediction)

326 - 352

MobiDB Lite
mobidb-lite (disorder_prediction)

Residue annotation

dimerization inter...

313H

dimerization interface
313H, 314F, 315L, 316A, 318V, 343D, 355A, 358A, 361A, 413Y, 436P, 437I, 441G
CDD
cd02203 (PurL_repeat1)

314F

dimerization interface
313H, 314F, 315L, 316A, 318V, 343D, 355A, 358A, 361A, 413Y, 436P, 437I, 441G
CDD
cd02203 (PurL_repeat1)

315L

dimerization interface
313H, 314F, 315L, 316A, 318V, 343D, 355A, 358A, 361A, 413Y, 436P, 437I, 441G
CDD
cd02203 (PurL_repeat1)

316A

dimerization interface
313H, 314F, 315L, 316A, 318V, 343D, 355A, 358A, 361A, 413Y, 436P, 437I, 441G
CDD
cd02203 (PurL_repeat1)

318V

dimerization interface
313H, 314F, 315L, 316A, 318V, 343D, 355A, 358A, 361A, 413Y, 436P, 437I, 441G
CDD
cd02203 (PurL_repeat1)

343D

dimerization interface
313H, 314F, 315L, 316A, 318V, 343D, 355A, 358A, 361A, 413Y, 436P, 437I, 441G
CDD
cd02203 (PurL_repeat1)

355A

dimerization interface
313H, 314F, 315L, 316A, 318V, 343D, 355A, 358A, 361A, 413Y, 436P, 437I, 441G
CDD
cd02203 (PurL_repeat1)

358A

dimerization interface
313H, 314F, 315L, 316A, 318V, 343D, 355A, 358A, 361A, 413Y, 436P, 437I, 441G
CDD
cd02203 (PurL_repeat1)

361A

dimerization interface
313H, 314F, 315L, 316A, 318V, 343D, 355A, 358A, 361A, 413Y, 436P, 437I, 441G
CDD
cd02203 (PurL_repeat1)

413Y

dimerization interface
313H, 314F, 315L, 316A, 318V, 343D, 355A, 358A, 361A, 413Y, 436P, 437I, 441G
CDD
cd02203 (PurL_repeat1)

436P

dimerization interface
313H, 314F, 315L, 316A, 318V, 343D, 355A, 358A, 361A, 413Y, 436P, 437I, 441G
CDD
cd02203 (PurL_repeat1)

437I

dimerization interface
313H, 314F, 315L, 316A, 318V, 343D, 355A, 358A, 361A, 413Y, 436P, 437I, 441G
CDD
cd02203 (PurL_repeat1)

441G

dimerization interface
313H, 314F, 315L, 316A, 318V, 343D, 355A, 358A, 361A, 413Y, 436P, 437I, 441G
CDD
cd02203 (PurL_repeat1)
ATP binding site c...

339G

ATP binding site
339G, 343D, 411A, 412G, 413Y
CDD
cd02203 (PurL_repeat1)

343D

ATP binding site
339G, 343D, 411A, 412G, 413Y
CDD
cd02203 (PurL_repeat1)

411A

ATP binding site
339G, 343D, 411A, 412G, 413Y
CDD
cd02203 (PurL_repeat1)

412G

ATP binding site
339G, 343D, 411A, 412G, 413Y
CDD
cd02203 (PurL_repeat1)

413Y

ATP binding site
339G, 343D, 411A, 412G, 413Y
CDD
cd02203 (PurL_repeat1)
dimerization inter...

727G

dimerization interface
727G, 728E, 729S, 730F, 732I, 757N, 770K, 773A, 776M, 811K, 829L, 830A, 834T
CDD
cd02204 (PurL_repeat2)

728E

dimerization interface
727G, 728E, 729S, 730F, 732I, 757N, 770K, 773A, 776M, 811K, 829L, 830A, 834T
CDD
cd02204 (PurL_repeat2)

729S

dimerization interface
727G, 728E, 729S, 730F, 732I, 757N, 770K, 773A, 776M, 811K, 829L, 830A, 834T
CDD
cd02204 (PurL_repeat2)

730F

dimerization interface
727G, 728E, 729S, 730F, 732I, 757N, 770K, 773A, 776M, 811K, 829L, 830A, 834T
CDD
cd02204 (PurL_repeat2)

732I

dimerization interface
727G, 728E, 729S, 730F, 732I, 757N, 770K, 773A, 776M, 811K, 829L, 830A, 834T
CDD
cd02204 (PurL_repeat2)

757N

dimerization interface
727G, 728E, 729S, 730F, 732I, 757N, 770K, 773A, 776M, 811K, 829L, 830A, 834T
CDD
cd02204 (PurL_repeat2)

770K

dimerization interface
727G, 728E, 729S, 730F, 732I, 757N, 770K, 773A, 776M, 811K, 829L, 830A, 834T
CDD
cd02204 (PurL_repeat2)

773A

dimerization interface
727G, 728E, 729S, 730F, 732I, 757N, 770K, 773A, 776M, 811K, 829L, 830A, 834T
CDD
cd02204 (PurL_repeat2)

776M

dimerization interface
727G, 728E, 729S, 730F, 732I, 757N, 770K, 773A, 776M, 811K, 829L, 830A, 834T
CDD
cd02204 (PurL_repeat2)

811K

dimerization interface
727G, 728E, 729S, 730F, 732I, 757N, 770K, 773A, 776M, 811K, 829L, 830A, 834T
CDD
cd02204 (PurL_repeat2)

829L

dimerization interface
727G, 728E, 729S, 730F, 732I, 757N, 770K, 773A, 776M, 811K, 829L, 830A, 834T
CDD
cd02204 (PurL_repeat2)

830A

dimerization interface
727G, 728E, 729S, 730F, 732I, 757N, 770K, 773A, 776M, 811K, 829L, 830A, 834T
CDD
cd02204 (PurL_repeat2)

834T

dimerization interface
727G, 728E, 729S, 730F, 732I, 757N, 770K, 773A, 776M, 811K, 829L, 830A, 834T
CDD
cd02204 (PurL_repeat2)
ATP binding site c...

753E

ATP binding site
753E, 757N, 809V, 810G, 811K
CDD
cd02204 (PurL_repeat2)

757N

ATP binding site
753E, 757N, 809V, 810G, 811K
CDD
cd02204 (PurL_repeat2)

809V

ATP binding site
753E, 757N, 809V, 810G, 811K
CDD
cd02204 (PurL_repeat2)

810G

ATP binding site
753E, 757N, 809V, 810G, 811K
CDD
cd02204 (PurL_repeat2)

811K

ATP binding site
753E, 757N, 809V, 810G, 811K
CDD
cd02204 (PurL_repeat2)
putative active si...

1,124G

putative active site
1,124G, 1,125F, 1,129D, 1,167C, 1,171Q, 1,250A, 1,251H, 1,313M, 1,315H, 1,316P, 1,317E, 1,318R
CDD
cd01740 (GATase1_FGAR_AT)

1,125F

putative active site
1,124G, 1,125F, 1,129D, 1,167C, 1,171Q, 1,250A, 1,251H, 1,313M, 1,315H, 1,316P, 1,317E, 1,318R
CDD
cd01740 (GATase1_FGAR_AT)

1,129D

putative active site
1,124G, 1,125F, 1,129D, 1,167C, 1,171Q, 1,250A, 1,251H, 1,313M, 1,315H, 1,316P, 1,317E, 1,318R
CDD
cd01740 (GATase1_FGAR_AT)

1,167C

putative active site
1,124G, 1,125F, 1,129D, 1,167C, 1,171Q, 1,250A, 1,251H, 1,313M, 1,315H, 1,316P, 1,317E, 1,318R
CDD
cd01740 (GATase1_FGAR_AT)

1,171Q

putative active site
1,124G, 1,125F, 1,129D, 1,167C, 1,171Q, 1,250A, 1,251H, 1,313M, 1,315H, 1,316P, 1,317E, 1,318R
CDD
cd01740 (GATase1_FGAR_AT)

1,250A

putative active site
1,124G, 1,125F, 1,129D, 1,167C, 1,171Q, 1,250A, 1,251H, 1,313M, 1,315H, 1,316P, 1,317E, 1,318R
CDD
cd01740 (GATase1_FGAR_AT)

1,251H

putative active site
1,124G, 1,125F, 1,129D, 1,167C, 1,171Q, 1,250A, 1,251H, 1,313M, 1,315H, 1,316P, 1,317E, 1,318R
CDD
cd01740 (GATase1_FGAR_AT)

1,313M

putative active site
1,124G, 1,125F, 1,129D, 1,167C, 1,171Q, 1,250A, 1,251H, 1,313M, 1,315H, 1,316P, 1,317E, 1,318R
CDD
cd01740 (GATase1_FGAR_AT)

1,315H

putative active site
1,124G, 1,125F, 1,129D, 1,167C, 1,171Q, 1,250A, 1,251H, 1,313M, 1,315H, 1,316P, 1,317E, 1,318R
CDD
cd01740 (GATase1_FGAR_AT)

1,316P

putative active site
1,124G, 1,125F, 1,129D, 1,167C, 1,171Q, 1,250A, 1,251H, 1,313M, 1,315H, 1,316P, 1,317E, 1,318R
CDD
cd01740 (GATase1_FGAR_AT)

1,317E

putative active site
1,124G, 1,125F, 1,129D, 1,167C, 1,171Q, 1,250A, 1,251H, 1,313M, 1,315H, 1,316P, 1,317E, 1,318R
CDD
cd01740 (GATase1_FGAR_AT)

1,318R

putative active site
1,124G, 1,125F, 1,129D, 1,167C, 1,171Q, 1,250A, 1,251H, 1,313M, 1,315H, 1,316P, 1,317E, 1,318R
CDD
cd01740 (GATase1_FGAR_AT)
catalytic triad cd...

1,167C

catalytic triad
1,167C, 1,315H, 1,317E
CDD
cd01740 (GATase1_FGAR_AT)

1,315H

catalytic triad
1,167C, 1,315H, 1,317E
CDD
cd01740 (GATase1_FGAR_AT)

1,317E

catalytic triad
1,167C, 1,315H, 1,317E
CDD
cd01740 (GATase1_FGAR_AT)

Protein sequence

>MCA_03194_1
MTLVALAGPKAYSSFRINALINNINHSLNSSAVVAIRSVYVHYVNVAPGSDMFTSSDSTKRDLLDSLLEYDTPADTSDPL
TQTLLTAIKNSSNDLPENSYILRVIPRPGTISPWSSKATNIAHICGLDRDVLRLERGIALVVQVRKGFPFEEYLKSGVFL
EFVYDRMTQTIPNHVPANEDLFSIHDPKPLVHVPRNGIPEANKTLGLALDQAEIDYLVDSFPARDPTDVELFMFAQVNSE
HCRHKIFNADWKIDGELKPYTLFKMIRNTHNTNPQYTISAYSDNAAVLQGAKAFFFAPDPKTLNYIQEPETQHFLAKVET
HNHPTAVSPFPGAATGSGGEIRDEGATGRGSRPKAGLAGFAVSDLLIPDFKQPWELDVGKPSHISSSLDIMIQGPIGAAA
FNNEFGRPNIAGYFRTLTTTVTNHSGKQEIRGFHKPIMVAGGVGTVRPQFALKNRGINPGSAIIVLGGPAMLIGLGGGAA
SSVASGDAAAHLDFASVQRGNPEMQRRAQMVIDTCVGLGSANPIQSIHDVGAGGLSNALTELVHDNDLGARFELRDIPNV
EPGMSPMEIWCCEAQERYVLAVSPNDLPVFEEICKRERAIYGVVGYATSEKRLVLTDKLLGTTPIDIDMSVLFGKPPRLQ
RNATTKPLRLTPFDSTLTSYQVSPVDAVSTALERVLLLPSVGSKSFLITIGDRSVTGLIARDQMVGPWQTPVADTAVTIS
SFGVVHGESFAIGERPTLALISPAASAKMAVAESLLNLYAADVQELERVKLSANWMSAASHEGEGAALYEAVQAIGLDLC
PALGIAIPVGKDSMSMKMKWDGKEVIAPLACNITAFGPVNDVRRTWTPQLRKIQDSKLVLVDLGLGKNRLGGSAVAQVFS
QVGNEAPTVEDNALLRSFLKGLERLHQTDIVTAYHDRSDGGLITTLVEMAFAGRVGLDISLNSPDSDVISALFSEELGAV
FQVTDVDAFVHTISQEGVPAAAVSVIGEINLQKDQYINITFNGSRVYSNTRAALQQIWSSTSNRMQKLRDNPKSADQEFS
NILDNSDPGLSYKLTFDPAENIVVNLISSKPKVAILREQGVNGHNEMAYAMMLAGFTPVDVHMSDLFSGRANLDDFVGLA
ACGGFSYGDVLGAGAGWAKSVLFHKELRAQFEHFFNDRRDTFALGVCNGCQFLSRLKAIIPGAENWPSFERNASEQYEAR
TVMIEVVDDATNPDVTASESSADNANSTSEQQSSIFFSGMRGSRFPVAVAHGEGRASFDSTETYKQFVKDNLTVLRFVDN
YGKTTERYPYNPNGSPDGITGIRTPNGRVLALMPHPERVTVLETNSWYPDGGQVPEGPWLRLFRNARRWVG

GO term prediction

Biological Process

GO:0006189 'de novo' IMP biosynthetic process

Molecular Function

GO:0004642 phosphoribosylformylglycinamidine synthase activity

Cellular Component

None predicted.

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Entry type

Status

Per-residue features