Protein
MIA_03662_1
Length
377 amino acids
Browser: contig04:1823047-1824232-
Protein function
| EGGNOG: | 0PFFR | FEN1 | Structure-specific nuclease with 5'-flap endonuclease and 5'-3' exonuclease activities involved in DNA replication and repair. During DNA replication, cleaves the 5'-overhanging flap structure that is generated by displacement synthesis when DNA polymerase encounters the 5'-end of a downstream Okazaki fragment. It enters the flap from the 5'-end and then tracks to cleave the flap base, leaving a nick for ligation. Also involved in the long patch base excision repair (LP-BER) pathway, by cleaving within the apurinic apyrimidinic (AP) site-terminated flap. Acts as a genome stabilization factor that prevents flaps from equilibrating into structurs that lead to duplications and deletions. Also possesses 5'-3' exonuclease activity on nicked or gapped double- stranded DNA, and exhibits RNase H activity. Also involved in replication and repair of rDNA and in repairing mitochondrial DNA (By similarity) |
|---|---|---|---|
| SGD closest match: | S000001596 | RAD27 | Flap endonuclease 1 |
| CGD closest match: | CAL0000190371 | RAD27 | Flap endonuclease 1 |
Protein alignments
| %id | Aln length | E-value | ||
|---|---|---|---|---|
| A0A0J9X3X9_GEOCN | 75.731% | 342 | 7.16e-168 | Flap endonuclease 1 OS=Geotrichum candidum GN=FEN1 PE=3 SV=1 |
| MCA_06097_1 | 76.316% | 342 | 1.72e-166 | MCA_06097_1 |
| A0A1E3PSX6_9ASCO | 68.915% | 341 | 2.57e-153 | Flap endonuclease 1 OS=Nadsonia fulvescens var. elongata DSM 6958 GN=FEN1 PE=3 SV=1 |
| A0A060SZC7_BLAAD | 69.412% | 340 | 2.69e-147 | Flap endonuclease 1 OS=Blastobotrys adeninivorans GN=FEN1 PE=3 SV=1 |
| FEN1_YARLI | 63.068% | 352 | 1.96e-136 | Flap endonuclease 1 OS=Yarrowia lipolytica (strain CLIB 122 / E 150) GN=FEN1 PE=3 SV=1 |
| UniRef50_Q6C116 | 63.068% | 352 | 4.54e-133 | Flap endonuclease 1 n=417 Tax=Eukaryota TaxID=2759 RepID=FEN1_YARLI |
| FEN1_CANAL | 62.463% | 341 | 2.49e-136 | Flap endonuclease 1 OS=Candida albicans (strain SC5314 / ATCC MYA-2876) GN=RAD27 PE=3 SV=1 |
| A0A1E4THF0_9ASCO | 64.620% | 342 | 4.79e-134 | Flap endonuclease 1 OS=Tortispora caseinolytica NRRL Y-17796 GN=FEN1 PE=3 SV=1 |
| A0A167C4F9_9ASCO | 66.032% | 315 | 3.06e-132 | Flap endonuclease 1 OS=Sugiyamaella lignohabitans GN=RAD27 PE=3 SV=1 |
| FEN1_YEAST | 58.140% | 344 | 2.36e-122 | Flap endonuclease 1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=RAD27 PE=1 SV=1 |
Mitochondrial localization by mitoprotII
Probability of mitochondrial location: 0.5634
Protein family membership
- XPG/Rad2 endonuclease (IPR006084)
- Flap endonuclease 1 (IPR023426)
Domains and repeats
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Domain
-
Domain
-
Domain
1
50
100
150
200
250
300
350
377
Detailed signature matches
no IPR
Unintegrated signatures
-
-
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cd09907 (H3TH_FEN1-Euk)
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mobidb-lite (disord...)
Residue annotation
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FEN1-PCNA interfac...
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metal binding site...
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active site cd09867
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3' dsDNA binding s...
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putative 5' ssDNA ...
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metal binding site...
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putative DNA bindi...
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putative metal bin...
Protein sequence
>MIA_03662_1 MSDNASTAIATREFKNLVGRKVAIDASMSLYQFLIAVRQGDGQQLTDSSGETTSHLVGMFYRTLRIVENGIKPVYVFDGK PPDLKGGELAKRLEQREKAQKAAEELKETGTTEQIAKFERRTVKVTRQQNDEAKRLLKLMGIPYIEAPGEAEAQCAALAR AKKVYAAASEDMDTLTFGSPVVLRYLTAMESKGLPITEITLDTVLRSLELDQDQFIDLCILLGCDYCESIRGVGPVTAFK LIKEHKTLENIIEHIKKTPKSKYKVPEDWPYEAARELFKHPDVQDASKMEIKWTPPDVEGLVEFLVKEKGFSEARVRKGT EKLTKENTGATQGRLEDFFKVKSAPQSSKDLKNANKRKKAAEDAKKASKRVKVTRKK
GO term prediction
Biological Process
GO:0006281 DNA repair
Molecular Function
GO:0003677 DNA binding
GO:0003824 catalytic activity
GO:0004518 nuclease activity
GO:0016788 hydrolase activity, acting on ester bonds
Cellular Component
None predicted.