MIA_03366_1

Protein

Length

495 amino acids

Protein function

EGGNOG:	0PFKN	ALD5	Aldehyde dehydrogenase
SGD closest match:	S000000875	ALD5	Aldehyde dehydrogenase 5, mitochondrial
CGD closest match:	CAL0000188934	ALD5	Aldehyde dehydrogenase (NAD(P)(+))

Protein alignments

	%id	Aln length
MCA_05354_1	83.434%	495	MCA_05354_1
A0A0J9X459_GEOCN	80.730%	493	Similar to Saccharomyces cerevisiae YER073W ALD5 Mitochondrial aldehyde dehydrogenase OS=Geotrichum candidum GN=BN980_GECA02s02694g PE=3 SV=1
A0A060T678_BLAAD	69.753%	486	ARAD1C17776p OS=Blastobotrys adeninivorans GN=GNLVRS02_ARAD1C17776g PE=3 SV=1
A0A1E3PQ22_9ASCO	71.941%	474	Aldehyde dehydrogenase OS=Nadsonia fulvescens var. elongata DSM 6958 GN=NADFUDRAFT_49830 PE=3 SV=1
A0A167DZ78_9ASCO	66.598%	485	Aldehyde dehydrogenase (NADP(+)) ALD4 OS=Sugiyamaella lignohabitans GN=ALD4 PE=3 SV=1
UniRef50_J7R5Z3	63.008%	492	Uncharacterized protein n=129 Tax=Eukaryota TaxID=2759 RepID=J7R5Z3_KAZNA
ALDH5_YEAST	63.061%	490	Aldehyde dehydrogenase 5, mitochondrial OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=ALD5 PE=1 SV=4
Q6CD79_YARLI	62.424%	495	YALI0C03025p OS=Yarrowia lipolytica (strain CLIB 122 / E 150) GN=YALI0_C03025g PE=3 SV=1
A0A1E4TLM3_9ASCO	62.146%	494	Uncharacterized protein OS=Tortispora caseinolytica NRRL Y-17796 GN=CANCADRAFT_43149 PE=3 SV=1
A0A1D8PGT5_CANAL	58.969%	485	Aldehyde dehydrogenase (NAD(P)(+)) OS=Candida albicans (strain SC5314 / ATCC MYA-2876) GN=ALD5 PE=3 SV=1

Mitochondrial localization by mitoprotII

Probability of mitochondrial location: 0.1244

Protein family membership

None predicted.

Domains and repeats

Domain
13 - 280
Aldehyde dehydrogenase N-terminal domain (IPR016162)
Domain
13 - 490
Aldehyde/histidinol dehydrogenase (IPR016161)
Aldehyde dehydrogenase domain (IPR015590)

1 50 100 150 200 250 300 350 400 450 495

Detailed signature matches

IPR016162

Aldehyde dehydrogenase N-terminal domain
1. G3DSA:3.40.60...
  
  13 - 280
  
  GENE3D
  G3DSA:3.40.605.10 (G3DSA:3.40.605.10)
IPR016161

Aldehyde/histidinol dehydrogenase
1. SSF53720 (ALDH-like)
  
  13 - 490
  
  SUPERFAMILY
  SSF53720 (ALDH-like)
IPR015590

Aldehyde dehydrogenase domain
1. PF00171 (Aldedh)
  
  27 - 487
  
  Pfam
  PF00171 (Aldedh)
IPR029510

Aldehyde dehydrogenase, glutamic acid active site
1. PS00687 (ALDEHYDE_D...)
  
  262 - 269
  
  PROSITE patterns
  PS00687 (ALDEHYDE_DEHYDR_GLU)
IPR016160

Aldehyde dehydrogenase, cysteine active site
1. PS00070 (ALDEHYDE_D...)
  
  290 - 301
  
  PROSITE patterns
  PS00070 (ALDEHYDE_DEHYDR_CYS)

no IPR

Unintegrated signatures

G3DSA:3.40.30...

281 - 472

GENE3D
G3DSA:3.40.309.10 (G3DSA:3.40.309.10)
cd07091 (ALDH_F1-2_...)

17 - 489

CDD
cd07091 (ALDH_F1-2_Ald2-like)

Residue annotation

NAD(P) binding sit...

161V

NAD(P) binding site
161V, 162I, 164W, 165N, 188K, 191E, 220Y, 221G, 224G, 225Q, 238F, 239T, 240G, 241S, 244T, 247H, 248V, 263E, 264L, 265G, 297C, 394E, 396F, 422L, 460F
CDD
cd07091 (ALDH_F1-2_Ald2-like)

162I

NAD(P) binding site
161V, 162I, 164W, 165N, 188K, 191E, 220Y, 221G, 224G, 225Q, 238F, 239T, 240G, 241S, 244T, 247H, 248V, 263E, 264L, 265G, 297C, 394E, 396F, 422L, 460F
CDD
cd07091 (ALDH_F1-2_Ald2-like)

164W

NAD(P) binding site
161V, 162I, 164W, 165N, 188K, 191E, 220Y, 221G, 224G, 225Q, 238F, 239T, 240G, 241S, 244T, 247H, 248V, 263E, 264L, 265G, 297C, 394E, 396F, 422L, 460F
CDD
cd07091 (ALDH_F1-2_Ald2-like)

165N

NAD(P) binding site
161V, 162I, 164W, 165N, 188K, 191E, 220Y, 221G, 224G, 225Q, 238F, 239T, 240G, 241S, 244T, 247H, 248V, 263E, 264L, 265G, 297C, 394E, 396F, 422L, 460F
CDD
cd07091 (ALDH_F1-2_Ald2-like)

188K

NAD(P) binding site
161V, 162I, 164W, 165N, 188K, 191E, 220Y, 221G, 224G, 225Q, 238F, 239T, 240G, 241S, 244T, 247H, 248V, 263E, 264L, 265G, 297C, 394E, 396F, 422L, 460F
CDD
cd07091 (ALDH_F1-2_Ald2-like)

191E

NAD(P) binding site
161V, 162I, 164W, 165N, 188K, 191E, 220Y, 221G, 224G, 225Q, 238F, 239T, 240G, 241S, 244T, 247H, 248V, 263E, 264L, 265G, 297C, 394E, 396F, 422L, 460F
CDD
cd07091 (ALDH_F1-2_Ald2-like)

220Y

NAD(P) binding site
161V, 162I, 164W, 165N, 188K, 191E, 220Y, 221G, 224G, 225Q, 238F, 239T, 240G, 241S, 244T, 247H, 248V, 263E, 264L, 265G, 297C, 394E, 396F, 422L, 460F
CDD
cd07091 (ALDH_F1-2_Ald2-like)

221G

NAD(P) binding site
161V, 162I, 164W, 165N, 188K, 191E, 220Y, 221G, 224G, 225Q, 238F, 239T, 240G, 241S, 244T, 247H, 248V, 263E, 264L, 265G, 297C, 394E, 396F, 422L, 460F
CDD
cd07091 (ALDH_F1-2_Ald2-like)

224G

NAD(P) binding site
161V, 162I, 164W, 165N, 188K, 191E, 220Y, 221G, 224G, 225Q, 238F, 239T, 240G, 241S, 244T, 247H, 248V, 263E, 264L, 265G, 297C, 394E, 396F, 422L, 460F
CDD
cd07091 (ALDH_F1-2_Ald2-like)

225Q

NAD(P) binding site
161V, 162I, 164W, 165N, 188K, 191E, 220Y, 221G, 224G, 225Q, 238F, 239T, 240G, 241S, 244T, 247H, 248V, 263E, 264L, 265G, 297C, 394E, 396F, 422L, 460F
CDD
cd07091 (ALDH_F1-2_Ald2-like)

238F

NAD(P) binding site
161V, 162I, 164W, 165N, 188K, 191E, 220Y, 221G, 224G, 225Q, 238F, 239T, 240G, 241S, 244T, 247H, 248V, 263E, 264L, 265G, 297C, 394E, 396F, 422L, 460F
CDD
cd07091 (ALDH_F1-2_Ald2-like)

239T

NAD(P) binding site
161V, 162I, 164W, 165N, 188K, 191E, 220Y, 221G, 224G, 225Q, 238F, 239T, 240G, 241S, 244T, 247H, 248V, 263E, 264L, 265G, 297C, 394E, 396F, 422L, 460F
CDD
cd07091 (ALDH_F1-2_Ald2-like)

240G

NAD(P) binding site
161V, 162I, 164W, 165N, 188K, 191E, 220Y, 221G, 224G, 225Q, 238F, 239T, 240G, 241S, 244T, 247H, 248V, 263E, 264L, 265G, 297C, 394E, 396F, 422L, 460F
CDD
cd07091 (ALDH_F1-2_Ald2-like)

241S

NAD(P) binding site
161V, 162I, 164W, 165N, 188K, 191E, 220Y, 221G, 224G, 225Q, 238F, 239T, 240G, 241S, 244T, 247H, 248V, 263E, 264L, 265G, 297C, 394E, 396F, 422L, 460F
CDD
cd07091 (ALDH_F1-2_Ald2-like)

244T

NAD(P) binding site
161V, 162I, 164W, 165N, 188K, 191E, 220Y, 221G, 224G, 225Q, 238F, 239T, 240G, 241S, 244T, 247H, 248V, 263E, 264L, 265G, 297C, 394E, 396F, 422L, 460F
CDD
cd07091 (ALDH_F1-2_Ald2-like)

247H

NAD(P) binding site
161V, 162I, 164W, 165N, 188K, 191E, 220Y, 221G, 224G, 225Q, 238F, 239T, 240G, 241S, 244T, 247H, 248V, 263E, 264L, 265G, 297C, 394E, 396F, 422L, 460F
CDD
cd07091 (ALDH_F1-2_Ald2-like)

248V

NAD(P) binding site
161V, 162I, 164W, 165N, 188K, 191E, 220Y, 221G, 224G, 225Q, 238F, 239T, 240G, 241S, 244T, 247H, 248V, 263E, 264L, 265G, 297C, 394E, 396F, 422L, 460F
CDD
cd07091 (ALDH_F1-2_Ald2-like)

263E

NAD(P) binding site
161V, 162I, 164W, 165N, 188K, 191E, 220Y, 221G, 224G, 225Q, 238F, 239T, 240G, 241S, 244T, 247H, 248V, 263E, 264L, 265G, 297C, 394E, 396F, 422L, 460F
CDD
cd07091 (ALDH_F1-2_Ald2-like)

264L

NAD(P) binding site
161V, 162I, 164W, 165N, 188K, 191E, 220Y, 221G, 224G, 225Q, 238F, 239T, 240G, 241S, 244T, 247H, 248V, 263E, 264L, 265G, 297C, 394E, 396F, 422L, 460F
CDD
cd07091 (ALDH_F1-2_Ald2-like)

265G

NAD(P) binding site
161V, 162I, 164W, 165N, 188K, 191E, 220Y, 221G, 224G, 225Q, 238F, 239T, 240G, 241S, 244T, 247H, 248V, 263E, 264L, 265G, 297C, 394E, 396F, 422L, 460F
CDD
cd07091 (ALDH_F1-2_Ald2-like)

297C

NAD(P) binding site
161V, 162I, 164W, 165N, 188K, 191E, 220Y, 221G, 224G, 225Q, 238F, 239T, 240G, 241S, 244T, 247H, 248V, 263E, 264L, 265G, 297C, 394E, 396F, 422L, 460F
CDD
cd07091 (ALDH_F1-2_Ald2-like)

394E

NAD(P) binding site
161V, 162I, 164W, 165N, 188K, 191E, 220Y, 221G, 224G, 225Q, 238F, 239T, 240G, 241S, 244T, 247H, 248V, 263E, 264L, 265G, 297C, 394E, 396F, 422L, 460F
CDD
cd07091 (ALDH_F1-2_Ald2-like)

396F

NAD(P) binding site
161V, 162I, 164W, 165N, 188K, 191E, 220Y, 221G, 224G, 225Q, 238F, 239T, 240G, 241S, 244T, 247H, 248V, 263E, 264L, 265G, 297C, 394E, 396F, 422L, 460F
CDD
cd07091 (ALDH_F1-2_Ald2-like)

422L

NAD(P) binding site
161V, 162I, 164W, 165N, 188K, 191E, 220Y, 221G, 224G, 225Q, 238F, 239T, 240G, 241S, 244T, 247H, 248V, 263E, 264L, 265G, 297C, 394E, 396F, 422L, 460F
CDD
cd07091 (ALDH_F1-2_Ald2-like)

460F

NAD(P) binding site
161V, 162I, 164W, 165N, 188K, 191E, 220Y, 221G, 224G, 225Q, 238F, 239T, 240G, 241S, 244T, 247H, 248V, 263E, 264L, 265G, 297C, 394E, 396F, 422L, 460F
CDD
cd07091 (ALDH_F1-2_Ald2-like)
catalytic residues...

165N

catalytic residues
165N, 263E, 294G, 297C
CDD
cd07091 (ALDH_F1-2_Ald2-like)

263E

catalytic residues
165N, 263E, 294G, 297C
CDD
cd07091 (ALDH_F1-2_Ald2-like)

294G

catalytic residues
165N, 263E, 294G, 297C
CDD
cd07091 (ALDH_F1-2_Ald2-like)

297C

catalytic residues
165N, 263E, 294G, 297C
CDD
cd07091 (ALDH_F1-2_Ald2-like)

Protein sequence

>MIA_03366_1
MAQSVNIKFPNGLSADIPTGLFINNEFVKSNDGKTFPVYNPSTEEQICEVYEANEADVDAAVDAAEAAFEDWGFGDSSRR
GRLLTKLADLIEEHSETLASIESLNNGKPMSLARIDVQLSADCIRSYAGWADKLYGDVLPAGDDNYFSYVRREPIGVCGQ
VIPWNFPLLMWAWKIGPALAAGNTVVLKSAESTPLSALYASTLVKEAGIPPGVLNIVSGYGQTGQYISSHMRIKKIAFTG
STATGRHVMKAAATSNLKKVTLELGGKSPNIVFADADLDKAVEFANMGIYFNSGEVCCAGSRIYVQDTIYDTFLEKLKAR
AEKNVVGDPFDDNTFQGPQTSKIQLDRILSYIEHGKNEGARVVTGGKRLDRPGYFIHPTIFADVTEDMKIVKEEIFGPVV
TITKFSTTDEVIKLANNTEYGLAAGVHTTNINKALHVSAKIHAGTVWVNTYNDFHHNVPFGGYGQSGIGRELGREGILES
YTQPKSVRVKLDMAA

GO term prediction

Biological Process

GO:0008152 metabolic process
GO:0055114 oxidation-reduction process

Molecular Function

GO:0016491 oxidoreductase activity

Cellular Component