MCA_05354_1

Protein

Length

494 amino acids

Gene name: ALD5B

Description: Aldehyde dehydrogenase

Browser: contigD:1112945-1114430-

RNA-seq: read pairs 293, FPKM 7.3, percentile rank 21.1% (100% = highest expression)

Protein function

Annotation:		ALD5B	Aldehyde dehydrogenase
KEGG:	K00128	ALDH	aldehyde dehydrogenase (NAD+) [EC:1.2.1.3]
EGGNOG:	0PFKN	ALD5	Aldehyde dehydrogenase
SGD closest match:	S000005901	ALD4	Potassium-activated aldehyde dehydrogenase, mitochondrial
CGD closest match:	CAL0000188934	ALD5	Aldehyde dehydrogenase (NAD(P)(+))

Protein alignments

	%id	Aln length
A0A0J9X459_GEOCN	84.76%	492	Similar to Saccharomyces cerevisiae YER073W ALD5 Mitochondrial aldehyde dehydrogenase OS=Geotrichum candidum GN=BN980_GECA02s02694g PE=3 SV=1
MIA_03366_1	83.43%	495	MIA_03366_1
A0A060T678_BLAAD	70.31%	485	ARAD1C17776p OS=Blastobotrys adeninivorans GN=GNLVRS02_ARAD1C17776g PE=3 SV=1
A0A1E3PQ22_9ASCO	71.67%	473	Aldehyde dehydrogenase OS=Nadsonia fulvescens var. elongata DSM 6958 GN=NADFUDRAFT_49830 PE=3 SV=1
A0A167DZ78_9ASCO	67.77%	484	Aldehyde dehydrogenase (NADP(+)) ALD4 OS=Sugiyamaella lignohabitans GN=ALD4 PE=3 SV=1
UniRef50_W9CDG4	62.74%	475	Aldehyde dehydrogenase n=19 Tax=cellular organisms TaxID=131567 RepID=W9CDG4_9HELO
ALDH4_YEAST	61.71%	491	Potassium-activated aldehyde dehydrogenase, mitochondrial OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=ALD4 PE=1 SV=2
Q6CD79_YARLI	62.14%	486	YALI0C03025p OS=Yarrowia lipolytica (strain CLIB 122 / E 150) GN=YALI0_C03025g PE=3 SV=1
A0A1E4TLM3_9ASCO	62.55%	494	Uncharacterized protein OS=Tortispora caseinolytica NRRL Y-17796 GN=CANCADRAFT_43149 PE=3 SV=1
A0A1D8PGT5_CANAL	59.30%	484	Aldehyde dehydrogenase (NAD(P)(+)) OS=Candida albicans (strain SC5314 / ATCC MYA-2876) GN=ALD5 PE=3 SV=1

Mitochondrial localization by mitoprotII

Probability of mitochondrial location: 0.0772

Protein family membership

None predicted.

Domains and repeats

Domain
13 - 279
Aldehyde dehydrogenase N-terminal domain (IPR016162)
Domain
13 - 489
Aldehyde/histidinol dehydrogenase (IPR016161)
Aldehyde dehydrogenase domain (IPR015590)

1 50 100 150 200 250 300 350 400 450 494

Detailed signature matches

IPR016162

Aldehyde dehydrogenase N-terminal domain
1. G3DSA:3.40.60...
  
  13 - 279
  
  GENE3D
  G3DSA:3.40.605.10 (G3DSA:3.40.605.10)
IPR016161

Aldehyde/histidinol dehydrogenase
1. SSF53720 (ALDH-like)
  
  13 - 489
  
  SUPERFAMILY
  SSF53720 (ALDH-like)
IPR015590

Aldehyde dehydrogenase domain
1. PF00171 (Aldedh)
  
  28 - 486
  
  Pfam
  PF00171 (Aldedh)
IPR029510

Aldehyde dehydrogenase, glutamic acid active site
1. PS00687 (ALDEHYDE_D...)
  
  261 - 268
  
  PROSITE patterns
  PS00687 (ALDEHYDE_DEHYDR_GLU)
IPR016160

Aldehyde dehydrogenase, cysteine active site
1. PS00070 (ALDEHYDE_D...)
  
  289 - 300
  
  PROSITE patterns
  PS00070 (ALDEHYDE_DEHYDR_CYS)

no IPR

Unintegrated signatures

G3DSA:3.40.30...

280 - 471

GENE3D
G3DSA:3.40.309.10 (G3DSA:3.40.309.10)
cd07091 (ALDH_F1-2_...)

17 - 488

CDD
cd07091 (ALDH_F1-2_Ald2-like)

Residue annotation

NAD(P) binding sit...

160V

NAD(P) binding site
160V, 161I, 163W, 164N, 187K, 190E, 219Y, 220G, 223G, 224Q, 237F, 238T, 239G, 240S, 243T, 246H, 247V, 262E, 263L, 264G, 296C, 393E, 395F, 421L, 459F
CDD
cd07091 (ALDH_F1-2_Ald2-like)

161I

NAD(P) binding site
160V, 161I, 163W, 164N, 187K, 190E, 219Y, 220G, 223G, 224Q, 237F, 238T, 239G, 240S, 243T, 246H, 247V, 262E, 263L, 264G, 296C, 393E, 395F, 421L, 459F
CDD
cd07091 (ALDH_F1-2_Ald2-like)

163W

NAD(P) binding site
160V, 161I, 163W, 164N, 187K, 190E, 219Y, 220G, 223G, 224Q, 237F, 238T, 239G, 240S, 243T, 246H, 247V, 262E, 263L, 264G, 296C, 393E, 395F, 421L, 459F
CDD
cd07091 (ALDH_F1-2_Ald2-like)

164N

NAD(P) binding site
160V, 161I, 163W, 164N, 187K, 190E, 219Y, 220G, 223G, 224Q, 237F, 238T, 239G, 240S, 243T, 246H, 247V, 262E, 263L, 264G, 296C, 393E, 395F, 421L, 459F
CDD
cd07091 (ALDH_F1-2_Ald2-like)

187K

NAD(P) binding site
160V, 161I, 163W, 164N, 187K, 190E, 219Y, 220G, 223G, 224Q, 237F, 238T, 239G, 240S, 243T, 246H, 247V, 262E, 263L, 264G, 296C, 393E, 395F, 421L, 459F
CDD
cd07091 (ALDH_F1-2_Ald2-like)

190E

NAD(P) binding site
160V, 161I, 163W, 164N, 187K, 190E, 219Y, 220G, 223G, 224Q, 237F, 238T, 239G, 240S, 243T, 246H, 247V, 262E, 263L, 264G, 296C, 393E, 395F, 421L, 459F
CDD
cd07091 (ALDH_F1-2_Ald2-like)

219Y

NAD(P) binding site
160V, 161I, 163W, 164N, 187K, 190E, 219Y, 220G, 223G, 224Q, 237F, 238T, 239G, 240S, 243T, 246H, 247V, 262E, 263L, 264G, 296C, 393E, 395F, 421L, 459F
CDD
cd07091 (ALDH_F1-2_Ald2-like)

220G

NAD(P) binding site
160V, 161I, 163W, 164N, 187K, 190E, 219Y, 220G, 223G, 224Q, 237F, 238T, 239G, 240S, 243T, 246H, 247V, 262E, 263L, 264G, 296C, 393E, 395F, 421L, 459F
CDD
cd07091 (ALDH_F1-2_Ald2-like)

223G

NAD(P) binding site
160V, 161I, 163W, 164N, 187K, 190E, 219Y, 220G, 223G, 224Q, 237F, 238T, 239G, 240S, 243T, 246H, 247V, 262E, 263L, 264G, 296C, 393E, 395F, 421L, 459F
CDD
cd07091 (ALDH_F1-2_Ald2-like)

224Q

NAD(P) binding site
160V, 161I, 163W, 164N, 187K, 190E, 219Y, 220G, 223G, 224Q, 237F, 238T, 239G, 240S, 243T, 246H, 247V, 262E, 263L, 264G, 296C, 393E, 395F, 421L, 459F
CDD
cd07091 (ALDH_F1-2_Ald2-like)

237F

NAD(P) binding site
160V, 161I, 163W, 164N, 187K, 190E, 219Y, 220G, 223G, 224Q, 237F, 238T, 239G, 240S, 243T, 246H, 247V, 262E, 263L, 264G, 296C, 393E, 395F, 421L, 459F
CDD
cd07091 (ALDH_F1-2_Ald2-like)

238T

NAD(P) binding site
160V, 161I, 163W, 164N, 187K, 190E, 219Y, 220G, 223G, 224Q, 237F, 238T, 239G, 240S, 243T, 246H, 247V, 262E, 263L, 264G, 296C, 393E, 395F, 421L, 459F
CDD
cd07091 (ALDH_F1-2_Ald2-like)

239G

NAD(P) binding site
160V, 161I, 163W, 164N, 187K, 190E, 219Y, 220G, 223G, 224Q, 237F, 238T, 239G, 240S, 243T, 246H, 247V, 262E, 263L, 264G, 296C, 393E, 395F, 421L, 459F
CDD
cd07091 (ALDH_F1-2_Ald2-like)

240S

NAD(P) binding site
160V, 161I, 163W, 164N, 187K, 190E, 219Y, 220G, 223G, 224Q, 237F, 238T, 239G, 240S, 243T, 246H, 247V, 262E, 263L, 264G, 296C, 393E, 395F, 421L, 459F
CDD
cd07091 (ALDH_F1-2_Ald2-like)

243T

NAD(P) binding site
160V, 161I, 163W, 164N, 187K, 190E, 219Y, 220G, 223G, 224Q, 237F, 238T, 239G, 240S, 243T, 246H, 247V, 262E, 263L, 264G, 296C, 393E, 395F, 421L, 459F
CDD
cd07091 (ALDH_F1-2_Ald2-like)

246H

NAD(P) binding site
160V, 161I, 163W, 164N, 187K, 190E, 219Y, 220G, 223G, 224Q, 237F, 238T, 239G, 240S, 243T, 246H, 247V, 262E, 263L, 264G, 296C, 393E, 395F, 421L, 459F
CDD
cd07091 (ALDH_F1-2_Ald2-like)

247V

NAD(P) binding site
160V, 161I, 163W, 164N, 187K, 190E, 219Y, 220G, 223G, 224Q, 237F, 238T, 239G, 240S, 243T, 246H, 247V, 262E, 263L, 264G, 296C, 393E, 395F, 421L, 459F
CDD
cd07091 (ALDH_F1-2_Ald2-like)

262E

NAD(P) binding site
160V, 161I, 163W, 164N, 187K, 190E, 219Y, 220G, 223G, 224Q, 237F, 238T, 239G, 240S, 243T, 246H, 247V, 262E, 263L, 264G, 296C, 393E, 395F, 421L, 459F
CDD
cd07091 (ALDH_F1-2_Ald2-like)

263L

NAD(P) binding site
160V, 161I, 163W, 164N, 187K, 190E, 219Y, 220G, 223G, 224Q, 237F, 238T, 239G, 240S, 243T, 246H, 247V, 262E, 263L, 264G, 296C, 393E, 395F, 421L, 459F
CDD
cd07091 (ALDH_F1-2_Ald2-like)

264G

NAD(P) binding site
160V, 161I, 163W, 164N, 187K, 190E, 219Y, 220G, 223G, 224Q, 237F, 238T, 239G, 240S, 243T, 246H, 247V, 262E, 263L, 264G, 296C, 393E, 395F, 421L, 459F
CDD
cd07091 (ALDH_F1-2_Ald2-like)

296C

NAD(P) binding site
160V, 161I, 163W, 164N, 187K, 190E, 219Y, 220G, 223G, 224Q, 237F, 238T, 239G, 240S, 243T, 246H, 247V, 262E, 263L, 264G, 296C, 393E, 395F, 421L, 459F
CDD
cd07091 (ALDH_F1-2_Ald2-like)

393E

NAD(P) binding site
160V, 161I, 163W, 164N, 187K, 190E, 219Y, 220G, 223G, 224Q, 237F, 238T, 239G, 240S, 243T, 246H, 247V, 262E, 263L, 264G, 296C, 393E, 395F, 421L, 459F
CDD
cd07091 (ALDH_F1-2_Ald2-like)

395F

NAD(P) binding site
160V, 161I, 163W, 164N, 187K, 190E, 219Y, 220G, 223G, 224Q, 237F, 238T, 239G, 240S, 243T, 246H, 247V, 262E, 263L, 264G, 296C, 393E, 395F, 421L, 459F
CDD
cd07091 (ALDH_F1-2_Ald2-like)

421L

NAD(P) binding site
160V, 161I, 163W, 164N, 187K, 190E, 219Y, 220G, 223G, 224Q, 237F, 238T, 239G, 240S, 243T, 246H, 247V, 262E, 263L, 264G, 296C, 393E, 395F, 421L, 459F
CDD
cd07091 (ALDH_F1-2_Ald2-like)

459F

NAD(P) binding site
160V, 161I, 163W, 164N, 187K, 190E, 219Y, 220G, 223G, 224Q, 237F, 238T, 239G, 240S, 243T, 246H, 247V, 262E, 263L, 264G, 296C, 393E, 395F, 421L, 459F
CDD
cd07091 (ALDH_F1-2_Ald2-like)
catalytic residues...

164N

catalytic residues
164N, 262E, 293G, 296C
CDD
cd07091 (ALDH_F1-2_Ald2-like)

262E

catalytic residues
164N, 262E, 293G, 296C
CDD
cd07091 (ALDH_F1-2_Ald2-like)

293G

catalytic residues
164N, 262E, 293G, 296C
CDD
cd07091 (ALDH_F1-2_Ald2-like)

296C

catalytic residues
164N, 262E, 293G, 296C
CDD
cd07091 (ALDH_F1-2_Ald2-like)

Protein sequence

>MCA_05354_1
MAESVNVKFPNGISADIPTGLFINNKFVPSADGKKFPVNNPTTEEVICEVYEAGEKDVDEAVNAAEAAFEAWGFGDSTKR
GRLLNDLADKFEEHAETLASIESLNNGKPMALARIDVQLSADTIRNYGGWANKIYGDVIDSGEEHFNYTRREPIGVCGQV
IPWNFPLLMFSWKIGPALAAGNCVVLKSAESTPLSALYAANLAKEVGFPPGVLNVVSGYGQTGQYITSHMKIKKIAFTGS
TATGRHVMKAAAASNLKKVTLELGGKSPNIVFDDAELDKAVEFANMGIFFNSGEVCCAGSRIYVQEGIYEKFLEKLKARA
EKNVVGDPFDDKTFQGPQTSKVQLDRILSYIEHGKQEGARVITGGKRVDRKGYFVHPTIFADVTEDMKIVKEEIFGPVVT
ITKFKDTEDVIKMANDTEYGLAAGIHTTNINKALHVANRIRAGTVWINTYNDFHHNVPFGGFNQSGIGRELGKEGIMEAY
TQPKSVRVKIDMGA

GO term prediction

Biological Process

GO:0008152 metabolic process
GO:0055114 oxidation-reduction process

Molecular Function

GO:0016491 oxidoreductase activity

Cellular Component