MIA_03361_1

Protein

Length

526 amino acids

Protein function

EGGNOG:	0PFKN	ALD5	Aldehyde dehydrogenase
SGD closest match:	S000000875	ALD5	Aldehyde dehydrogenase 5, mitochondrial
CGD closest match:	CAL0000188934	ALD5	Aldehyde dehydrogenase (NAD(P)(+))

Protein alignments

	%id	Aln length
MCA_05381_1	75.449%	501	MCA_05381_1
A0A0J9XHI0_GEOCN	72.600%	500	Similar to Saccharomyces cerevisiae YOR374W ALD4 Mitochondrial aldehyde dehydrogenase OS=Geotrichum candidum GN=BN980_GECA18s01209g PE=3 SV=1
A0A060T678_BLAAD	64.182%	483	ARAD1C17776p OS=Blastobotrys adeninivorans GN=GNLVRS02_ARAD1C17776g PE=3 SV=1
ALDH5_YEAST	64.000%	500	Aldehyde dehydrogenase 5, mitochondrial OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=ALD5 PE=1 SV=4
A0A1E3PQ22_9ASCO	67.158%	475	Aldehyde dehydrogenase OS=Nadsonia fulvescens var. elongata DSM 6958 GN=NADFUDRAFT_49830 PE=3 SV=1
UniRef50_P46367	62.626%	495	Potassium-activated aldehyde dehydrogenase, mitochondrial n=71 Tax=Saccharomycetales TaxID=4892 RepID=ALDH4_YEAST
A0A167DZ78_9ASCO	65.618%	477	Aldehyde dehydrogenase (NADP(+)) ALD4 OS=Sugiyamaella lignohabitans GN=ALD4 PE=3 SV=1
A0A1D8PGT5_CANAL	62.702%	496	Aldehyde dehydrogenase (NAD(P)(+)) OS=Candida albicans (strain SC5314 / ATCC MYA-2876) GN=ALD5 PE=3 SV=1
Q6C7J6_YARLI	62.626%	495	YALI0E00264p OS=Yarrowia lipolytica (strain CLIB 122 / E 150) GN=YALI0_E00264g PE=3 SV=1
A0A1E4TLM3_9ASCO	58.537%	492	Uncharacterized protein OS=Tortispora caseinolytica NRRL Y-17796 GN=CANCADRAFT_43149 PE=3 SV=1

Mitochondrial localization by mitoprotII

Probability of mitochondrial location: 0.9969
Predicted cleavage: 30

Protein family membership

None predicted.

Domains and repeats

Domain
41 - 522
Aldehyde/histidinol dehydrogenase (IPR016161)
Aldehyde dehydrogenase domain (IPR015590)
Domain
46 - 312
Aldehyde dehydrogenase N-terminal domain (IPR016162)

1 50 100 150 200 250 300 350 400 450 500 526

Detailed signature matches

IPR016161

Aldehyde/histidinol dehydrogenase
1. SSF53720 (ALDH-like)
  
  41 - 522
  
  SUPERFAMILY
  SSF53720 (ALDH-like)
IPR016162

Aldehyde dehydrogenase N-terminal domain
1. G3DSA:3.40.60...
  
  46 - 312
  
  GENE3D
  G3DSA:3.40.605.10 (G3DSA:3.40.605.10)
IPR015590

Aldehyde dehydrogenase domain
1. PF00171 (Aldedh)
  
  60 - 518
  
  Pfam
  PF00171 (Aldedh)
IPR029510

Aldehyde dehydrogenase, glutamic acid active site
1. PS00687 (ALDEHYDE_D...)
  
  294 - 301
  
  PROSITE patterns
  PS00687 (ALDEHYDE_DEHYDR_GLU)
IPR016160

Aldehyde dehydrogenase, cysteine active site
1. PS00070 (ALDEHYDE_D...)
  
  322 - 333
  
  PROSITE patterns
  PS00070 (ALDEHYDE_DEHYDR_CYS)

no IPR

Unintegrated signatures

G3DSA:3.40.30...

313 - 504

GENE3D
G3DSA:3.40.309.10 (G3DSA:3.40.309.10)
NON_CYTOPLASM... (N...)

18 - 526

PHOBIUS
NON_CYTOPLASMIC_DOMAIN (Non cytoplasmic domain)
SIGNAL_PEPTIDE (Sig...)

1 - 17

PHOBIUS
SIGNAL_PEPTIDE (Signal Peptide)
SIGNAL_PEPTID... (S...)

13 - 17

PHOBIUS
SIGNAL_PEPTIDE_C_REGION (Signal peptide C-region)
SIGNAL_PEPTID... (S...)

4 - 12

PHOBIUS
SIGNAL_PEPTIDE_H_REGION (Signal peptide H-region)
SIGNAL_PEPTID... (S...)

1 - 3

PHOBIUS
SIGNAL_PEPTIDE_N_REGION (Signal peptide N-region)
cd07091 (ALDH_F1-2_...)

49 - 520

CDD
cd07091 (ALDH_F1-2_Ald2-like)

Residue annotation

NAD(P) binding sit...

193I

NAD(P) binding site
193I, 194I, 196W, 197N, 220K, 223E, 252F, 253G, 256G, 257A, 270F, 271T, 272G, 273S, 276T, 279Y, 280I, 295E, 296L, 297G, 329C, 426E, 428F, 454L, 492F
CDD
cd07091 (ALDH_F1-2_Ald2-like)

194I

NAD(P) binding site
193I, 194I, 196W, 197N, 220K, 223E, 252F, 253G, 256G, 257A, 270F, 271T, 272G, 273S, 276T, 279Y, 280I, 295E, 296L, 297G, 329C, 426E, 428F, 454L, 492F
CDD
cd07091 (ALDH_F1-2_Ald2-like)

196W

NAD(P) binding site
193I, 194I, 196W, 197N, 220K, 223E, 252F, 253G, 256G, 257A, 270F, 271T, 272G, 273S, 276T, 279Y, 280I, 295E, 296L, 297G, 329C, 426E, 428F, 454L, 492F
CDD
cd07091 (ALDH_F1-2_Ald2-like)

197N

NAD(P) binding site
193I, 194I, 196W, 197N, 220K, 223E, 252F, 253G, 256G, 257A, 270F, 271T, 272G, 273S, 276T, 279Y, 280I, 295E, 296L, 297G, 329C, 426E, 428F, 454L, 492F
CDD
cd07091 (ALDH_F1-2_Ald2-like)

220K

NAD(P) binding site
193I, 194I, 196W, 197N, 220K, 223E, 252F, 253G, 256G, 257A, 270F, 271T, 272G, 273S, 276T, 279Y, 280I, 295E, 296L, 297G, 329C, 426E, 428F, 454L, 492F
CDD
cd07091 (ALDH_F1-2_Ald2-like)

223E

NAD(P) binding site
193I, 194I, 196W, 197N, 220K, 223E, 252F, 253G, 256G, 257A, 270F, 271T, 272G, 273S, 276T, 279Y, 280I, 295E, 296L, 297G, 329C, 426E, 428F, 454L, 492F
CDD
cd07091 (ALDH_F1-2_Ald2-like)

252F

NAD(P) binding site
193I, 194I, 196W, 197N, 220K, 223E, 252F, 253G, 256G, 257A, 270F, 271T, 272G, 273S, 276T, 279Y, 280I, 295E, 296L, 297G, 329C, 426E, 428F, 454L, 492F
CDD
cd07091 (ALDH_F1-2_Ald2-like)

253G

NAD(P) binding site
193I, 194I, 196W, 197N, 220K, 223E, 252F, 253G, 256G, 257A, 270F, 271T, 272G, 273S, 276T, 279Y, 280I, 295E, 296L, 297G, 329C, 426E, 428F, 454L, 492F
CDD
cd07091 (ALDH_F1-2_Ald2-like)

256G

NAD(P) binding site
193I, 194I, 196W, 197N, 220K, 223E, 252F, 253G, 256G, 257A, 270F, 271T, 272G, 273S, 276T, 279Y, 280I, 295E, 296L, 297G, 329C, 426E, 428F, 454L, 492F
CDD
cd07091 (ALDH_F1-2_Ald2-like)

257A

NAD(P) binding site
193I, 194I, 196W, 197N, 220K, 223E, 252F, 253G, 256G, 257A, 270F, 271T, 272G, 273S, 276T, 279Y, 280I, 295E, 296L, 297G, 329C, 426E, 428F, 454L, 492F
CDD
cd07091 (ALDH_F1-2_Ald2-like)

270F

NAD(P) binding site
193I, 194I, 196W, 197N, 220K, 223E, 252F, 253G, 256G, 257A, 270F, 271T, 272G, 273S, 276T, 279Y, 280I, 295E, 296L, 297G, 329C, 426E, 428F, 454L, 492F
CDD
cd07091 (ALDH_F1-2_Ald2-like)

271T

NAD(P) binding site
193I, 194I, 196W, 197N, 220K, 223E, 252F, 253G, 256G, 257A, 270F, 271T, 272G, 273S, 276T, 279Y, 280I, 295E, 296L, 297G, 329C, 426E, 428F, 454L, 492F
CDD
cd07091 (ALDH_F1-2_Ald2-like)

272G

NAD(P) binding site
193I, 194I, 196W, 197N, 220K, 223E, 252F, 253G, 256G, 257A, 270F, 271T, 272G, 273S, 276T, 279Y, 280I, 295E, 296L, 297G, 329C, 426E, 428F, 454L, 492F
CDD
cd07091 (ALDH_F1-2_Ald2-like)

273S

NAD(P) binding site
193I, 194I, 196W, 197N, 220K, 223E, 252F, 253G, 256G, 257A, 270F, 271T, 272G, 273S, 276T, 279Y, 280I, 295E, 296L, 297G, 329C, 426E, 428F, 454L, 492F
CDD
cd07091 (ALDH_F1-2_Ald2-like)

276T

NAD(P) binding site
193I, 194I, 196W, 197N, 220K, 223E, 252F, 253G, 256G, 257A, 270F, 271T, 272G, 273S, 276T, 279Y, 280I, 295E, 296L, 297G, 329C, 426E, 428F, 454L, 492F
CDD
cd07091 (ALDH_F1-2_Ald2-like)

279Y

NAD(P) binding site
193I, 194I, 196W, 197N, 220K, 223E, 252F, 253G, 256G, 257A, 270F, 271T, 272G, 273S, 276T, 279Y, 280I, 295E, 296L, 297G, 329C, 426E, 428F, 454L, 492F
CDD
cd07091 (ALDH_F1-2_Ald2-like)

280I

NAD(P) binding site
193I, 194I, 196W, 197N, 220K, 223E, 252F, 253G, 256G, 257A, 270F, 271T, 272G, 273S, 276T, 279Y, 280I, 295E, 296L, 297G, 329C, 426E, 428F, 454L, 492F
CDD
cd07091 (ALDH_F1-2_Ald2-like)

295E

NAD(P) binding site
193I, 194I, 196W, 197N, 220K, 223E, 252F, 253G, 256G, 257A, 270F, 271T, 272G, 273S, 276T, 279Y, 280I, 295E, 296L, 297G, 329C, 426E, 428F, 454L, 492F
CDD
cd07091 (ALDH_F1-2_Ald2-like)

296L

NAD(P) binding site
193I, 194I, 196W, 197N, 220K, 223E, 252F, 253G, 256G, 257A, 270F, 271T, 272G, 273S, 276T, 279Y, 280I, 295E, 296L, 297G, 329C, 426E, 428F, 454L, 492F
CDD
cd07091 (ALDH_F1-2_Ald2-like)

297G

NAD(P) binding site
193I, 194I, 196W, 197N, 220K, 223E, 252F, 253G, 256G, 257A, 270F, 271T, 272G, 273S, 276T, 279Y, 280I, 295E, 296L, 297G, 329C, 426E, 428F, 454L, 492F
CDD
cd07091 (ALDH_F1-2_Ald2-like)

329C

NAD(P) binding site
193I, 194I, 196W, 197N, 220K, 223E, 252F, 253G, 256G, 257A, 270F, 271T, 272G, 273S, 276T, 279Y, 280I, 295E, 296L, 297G, 329C, 426E, 428F, 454L, 492F
CDD
cd07091 (ALDH_F1-2_Ald2-like)

426E

NAD(P) binding site
193I, 194I, 196W, 197N, 220K, 223E, 252F, 253G, 256G, 257A, 270F, 271T, 272G, 273S, 276T, 279Y, 280I, 295E, 296L, 297G, 329C, 426E, 428F, 454L, 492F
CDD
cd07091 (ALDH_F1-2_Ald2-like)

428F

NAD(P) binding site
193I, 194I, 196W, 197N, 220K, 223E, 252F, 253G, 256G, 257A, 270F, 271T, 272G, 273S, 276T, 279Y, 280I, 295E, 296L, 297G, 329C, 426E, 428F, 454L, 492F
CDD
cd07091 (ALDH_F1-2_Ald2-like)

454L

NAD(P) binding site
193I, 194I, 196W, 197N, 220K, 223E, 252F, 253G, 256G, 257A, 270F, 271T, 272G, 273S, 276T, 279Y, 280I, 295E, 296L, 297G, 329C, 426E, 428F, 454L, 492F
CDD
cd07091 (ALDH_F1-2_Ald2-like)

492F

NAD(P) binding site
193I, 194I, 196W, 197N, 220K, 223E, 252F, 253G, 256G, 257A, 270F, 271T, 272G, 273S, 276T, 279Y, 280I, 295E, 296L, 297G, 329C, 426E, 428F, 454L, 492F
CDD
cd07091 (ALDH_F1-2_Ald2-like)
catalytic residues...

197N

catalytic residues
197N, 295E, 326G, 329C
CDD
cd07091 (ALDH_F1-2_Ald2-like)

295E

catalytic residues
197N, 295E, 326G, 329C
CDD
cd07091 (ALDH_F1-2_Ald2-like)

326G

catalytic residues
197N, 295E, 326G, 329C
CDD
cd07091 (ALDH_F1-2_Ald2-like)

329C

catalytic residues
197N, 295E, 326G, 329C
CDD
cd07091 (ALDH_F1-2_Ald2-like)

Protein sequence

>MIA_03361_1
MSRSLTQAARALKNASAARSVFAHTIRCYSASALPLKTPITLPNGINYEQPTGLFINNEFVASQSGKTFSVESPSTQHTV
AEVYEGGEADVDIAVKAAEEAFKSWSQTPPSERGLLLSKLADKVHENAELLASIESFDNGKALTLARGDVALVEKVIRYY
AGIADKIYGTQIPTNDGFFTYTAREPLGVCGQIIPWNFPLLMWSWKIGPALATGNTVVLKTAESTPLSALVVSQFVKEVG
IPAGVVNIISGFGPTGAAIAKHPRIKKVAFTGSTATGRYIMKAAAESNLKKVTLELGGKSPHIIFDDADLDTAVEVAALG
IFYNSGEVCSAGSRLYVQDTIYDEFINRLKARAEAISVGDPFDNETFQGSQTSKAQLDKILKYINLGKEEGARLVTGGER
LDRPGYFIAPTVFADVKEDMRIVREEIFGPVIVATKFSSVAEVVDLANNSEYGLGAGLHTEDINKAIDVAGRIHSGSVWV
NTYNAFHEAVPFGGFNQSGMGREMGEQSIDNYTQTKAVRIKINPKN

GO term prediction

Biological Process

GO:0008152 metabolic process
GO:0055114 oxidation-reduction process

Molecular Function

GO:0016491 oxidoreductase activity

Cellular Component