Protein

MIA_03338_1

Length
2,331 amino acids

Browser: contig04:911626-918769+

Protein function

EGGNOG:0PGZDPOL2DNA polymerase
SGD closest match:S000005206POL2DNA polymerase epsilon catalytic subunit A
CGD closest match:CAL0000195005POL2DNA polymerase epsilon catalytic subunit

Protein alignments

%idAln lengthE-value
MCA_06247_169.425%23320.0MCA_06247_1
A0A0J9XFD1_GEOCN60.603%23200.0Similar to Saccharomyces cerevisiae YNL262W POL2 Catalytic subunit of DNA polymerase (II) epsilon OS=Geotrichum candidum GN=BN980_GECA11s03783g PE=4 SV=1
A0A060TA90_BLAAD54.810%23390.0ARAD1D21846p OS=Blastobotrys adeninivorans GN=GNLVRS02_ARAD1D21846g PE=4 SV=1
A0A1E3PE40_9ASCO56.596%20620.0DNA polymerase epsilon catalytic subunit A OS=Nadsonia fulvescens var. elongata DSM 6958 GN=NADFUDRAFT_53495 PE=4 SV=1
UniRef50_Q4WXH851.234%23500.0DNA polymerase epsilon catalytic subunit A n=48 Tax=leotiomyceta TaxID=716546 RepID=DPOE_ASPFU
A0A1D8PTD0_CANAL49.656%23260.0DNA polymerase epsilon catalytic subunit OS=Candida albicans (strain SC5314 / ATCC MYA-2876) GN=POL2 PE=4 SV=1
A0A167C349_9ASCO54.300%20700.0DNA polymerase epsilon catalytic subunit OS=Sugiyamaella lignohabitans GN=POL2 PE=4 SV=1
DPOE_YEAST47.353%23420.0DNA polymerase epsilon catalytic subunit A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=POL2 PE=1 SV=1
A0A1E4TE88_9ASCO50.665%20310.0Uncharacterized protein OS=Tortispora caseinolytica NRRL Y-17796 GN=CANCADRAFT_1791 PE=4 SV=1
DPOE_YARLI41.777%23410.0DNA polymerase epsilon catalytic subunit A OS=Yarrowia lipolytica (strain CLIB 122 / E 150) GN=POL2 PE=3 SV=1

Mitochondrial localization by mitoprotII

Probability of mitochondrial location: 0.5549

Protein family membership

Domains and repeats

  1. Domain
  2. Domain
  3. Domain
1 500 1000 1500 2000 2331

Detailed signature matches

  1. Family IPR006172
    DNA-directed DNA polymerase, family B
    1. SM00486 (polmehr3)
  2. Domain IPR012337
    Ribonuclease H-like domain
    1. SSF53098 (Ribonucle...)
    2. G3DSA:3.30.42...
  3. Domain IPR006133
    DNA-directed DNA polymerase, family B, exonuclease domain
    1. PF03104 (DNA_pol_B_...)
  4. Domain IPR006134
    DNA-directed DNA polymerase, family B, multifunctional domain
    1. PF00136 (DNA_pol_B)
  5. Domain IPR023211
    DNA polymerase, palm domain
    1. G3DSA:3.90.16...
  6. Domain IPR013697
    DNA polymerase epsilon, catalytic subunit A, C-terminal
    1. PF08490 (DUF1744)
    2. SM01159 (DUF1744_2)
Unintegrated signatures no IPR
Unintegrated signatures
  1. Coil
  2. G3DSA:3.30.34...
  3. SSF56672 (DNA/RNA p...)
  4. cd05535 (POLBc_epsilon)
  5. cd05779 (DNA_polB_e...)
  6. mobidb-lite (disord...)

Residue annotation

  1. catalytic site cd0...
  2. active site cd05779
  3. substrate binding ...
  4. active site cd05535
  5. metal-binding site...

Protein sequence

>MIA_03338_1
MPNPSAQRNGRSMRSYKPSSKPSISGIGLPMDATSVSQKFEGVIETDHLDDKLGFPRYDSGPKKVGWLVNMHSTTRQLDN
SPVNGNAGVDYYFLDEEGGGFKATVLFDPYFLIICKKGYEAEIEEYLKKVLEGVLKNIKKIVKEDLQLPNHLLGNKQTLI
QLTFHNVTDLLAARRIIAPLVEKNKSKKDIKDTYEEIALSSINVDFDDLNTDKKLIQDPSELIYEIREYDVPYHVRVAID
MDIRVGKWYTVESKSGVIQLTEMPEREIRADPVVLAFDIETTKLPLKFPDAAIDKVMMISYMIDGEGFLITNREIVSQDI
DDFEYTPKPEYPGVFTIFNEPDEKALLERFFEHVQEAKPSVVATFNGDFFDWPFVEKRAAVHGIDMYTEIGFKIDSEEEY
KSTQCVHMDCFRWVKRDSYLPQGSQGLKAVTTAKLGYNPIEIDPELMTPYAIEKPQTMAEYSVSDAVATYYLYMKYVHPF
IFSLCNIIPLNPDEVLRKGTGTLCEMLLMVQAYKGSIVLPNKHTDPLERFYKGHLLETETYVGGHVESLEAGVFRSDIPA
QFEVDTSAVDELLRDLDNALVFSIEVEASKKLEDVTNYDEVKQKITDALMILKNEPKRNEKPSIYHVDVASMYPNIMTTN
RLQPDSMITEEDCAACDFNRPGKNCDRRLPWAWRGEFFPAKLEEYMMIRRALENEVFPAKSPNAPPIPFTSLSASEQASQ
IKKRLTEYSRKVYHRVHKTETIEREAIICQRENPFYVNTVRDFRDRRYHFKTLQKVWKKKAGEVPANDAAGKEDAKKMIV
LYDSLQLAHKVILNSFYGYVMRKGSRWYSMEMAGVTCLTGATIIQLARSLVERLGRPLELDTDGIWCILPGSFPENFTFD
LKDGKKIFISYPCVMLNHLVHAKFTNHQYQTLIDSKTHSYSTMSDNSIFFEVDGPYKAMVLPTSKEEGKGLKKRYAVFND
DGSLAELKGFELKRRGELKLIKTFQSQIFKEFLQGTTLKECYGAVARVADSWLDILESKGATLDREELLELISENKSMSK
SLEEYGNQKSTSITTAGRLAEFLGASMVKDRGLNCKYIIADRPAGAPVTERAIPVAIFSSERNVKSHFLRKWLKDSSLEE
FDPRSIIDWGYYWERLASTIQKIITIPAGLQNVENPVPRVPHPEWLEKRVRQQTQNFKQKSLSNYFKKTKGPAPFANGNF
SESQIGDIEDIGNGSSKTNAAVSAQPKMAKVTRVSKRRNDASKTTQPDEEFEKLPDKVPPIDGEYSDWLSYQKKMWKIQK
AERLRRRQVFGTSGSAVSSIRATGIASMMQNQVQQAFGSGIWNIVQILNNSSKPGEVRVTVSIGGKLQNVRVKVPRKIYV
NFKTGFIVSDLEKELPNCKVEKAPNSVLPDGSTSTLLYRLTMTEEVYLDEIDKPTSILKHPLVEGIYESKIDSSQNMVLD
IGLACSLSNKQPGLLGKGLASGFELEWLSPYYDKSSIHIKAKHDYLDSVSFSYIHIIHVSFLDHHFVIIIPTWSSKAHIL
ISRPIAQGQPFPNLSRIYTSLLAERNFNIQQHGDDKRKLFEYPNSMIFEETTFNSLTRLFQRANKILEALQSEKGIQTIV
ALQSPTPDRFKGMVRTVNDFPICQIRATNTSSFPPLGWHQIAAKKMIAQFFGLNKWLVHLHDLAKYSKIPLCNLQWDDMR
YVIDVNYARRLRAAGLVLWWSSTPLPDEGGKENDAMALSEIDGLDLPVVNNPGLYTTVCLDLSMRNLVINTVLTASLLMN
AEGGGGSSGNSISMTTEYENGQVEETVVPFVEGAFSTPAVTALSSMVKGWWNQALKEDESSPLAMFSDAMVNSFVSWVSS
RDSFMYHRTLLQHVNTLTQKTFIQLLREVRKSGSRLVFADQSHLVLLTSKSSVETSYAYANYLIKTIHSKPFFQFLDLEI
SCYWGRLLWMDDVNFGGLGCVEIDATSSNEVPLELALKWDIKGFLPPRLALEFDNWITKFLEVVDDKVRSYDPHASADSD
SDNEKPVRATQIPHSSEDNSKMLKGVVQSLEKQFLSRIRALIQKQHFCMKVNIEEAIAEGNEEDIASYKSFNTVSHPGSL
AQAFTFATTTLGIPIMSGAGVSTNGYNGQVTAMGNSYIHNQNITNNNVLQLVKFVCAIFSLAKSIELEVRLFRRHALSIF
EIREFSTEGNFSNPTASLRVSNFSCSNCGDVRDVDLCRGVSERIQYAYKLWQRAQKQQQETTNNPLDDTAFGKSMSLGEC
MECGHPYSRLQIEEQLIQQVLRLLTQYQVQDLKCSRCKRVREDDMSPHCPCSGDWVESLPRDEVARKIAIYAHSAGFYGL
KLLSGFIEDLF

GO term prediction

Biological Process

GO:0006260 DNA replication

Molecular Function

GO:0000166 nucleotide binding
GO:0003676 nucleic acid binding
GO:0003677 DNA binding
GO:0003887 DNA-directed DNA polymerase activity
GO:0008270 zinc ion binding
GO:0008408 3'-5' exonuclease activity

Cellular Component

GO:0005634 nucleus