Protein

MCA_06247_1

Length
2,291 amino acids

Gene name: POL2

Description: DNA polymerase epsilon catalytic subunit A

Browser: contigD:3665791-3672845+

RNA-seq: read pairs 606, FPKM 3.3, percentile rank 14.2% (100% = highest expression)

Protein function

Annotation:POL2DNA polymerase epsilon catalytic subunit A
KEGG:K02324POLE1 DNA polymerase epsilon subunit 1 [EC:2.7.7.7]
EGGNOG:0PGZDPOL2DNA polymerase
SGD closest match:S000005206POL2DNA polymerase epsilon catalytic subunit A
CGD closest match:CAL0000195005POL2DNA polymerase epsilon catalytic subunit

Protein alignments

%idAln lengthE-value
MIA_03338_169.48%23300.0MIA_03338_1
A0A0J9XFD1_GEOCN60.41%23010.0Similar to Saccharomyces cerevisiae YNL262W POL2 Catalytic subunit of DNA polymerase (II) epsilon OS=Geotrichum candidum GN=BN980_GECA11s03783g PE=4 SV=1
A0A1E3PE40_9ASCO54.65%22890.0DNA polymerase epsilon catalytic subunit A OS=Nadsonia fulvescens var. elongata DSM 6958 GN=NADFUDRAFT_53495 PE=4 SV=1
A0A060TA90_BLAAD54.53%23180.0ARAD1D21846p OS=Blastobotrys adeninivorans GN=GNLVRS02_ARAD1D21846g PE=4 SV=1
UniRef50_Q6BNG251.66%23130.0DNA polymerase epsilon catalytic subunit A n=179 Tax=Fungi TaxID=4751 RepID=DPOE_DEBHA
A0A1D8PTD0_CANAL51.72%22970.0DNA polymerase epsilon catalytic subunit OS=Candida albicans (strain SC5314 / ATCC MYA-2876) GN=POL2 PE=4 SV=1
A0A167C349_9ASCO55.69%20490.0DNA polymerase epsilon catalytic subunit OS=Sugiyamaella lignohabitans GN=POL2 PE=4 SV=1
DPOE_YEAST48.55%23090.0DNA polymerase epsilon catalytic subunit A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=POL2 PE=1 SV=1
A0A1E4TE88_9ASCO49.21%22780.0Uncharacterized protein OS=Tortispora caseinolytica NRRL Y-17796 GN=CANCADRAFT_1791 PE=4 SV=1
DPOE_YARLI40.92%23070.0DNA polymerase epsilon catalytic subunit A OS=Yarrowia lipolytica (strain CLIB 122 / E 150) GN=POL2 PE=3 SV=1

Mitochondrial localization by mitoprotII

Probability of mitochondrial location: 0.0203

Protein family membership

Domains and repeats

  1. Domain
  2. Domain
1 500 1000 1500 2000 2291

Detailed signature matches

  1. Family IPR006172
    DNA-directed DNA polymerase, family B
    1. SM00486 (polmehr3)
  2. Domain IPR012337
    Ribonuclease H-like domain
    1. SSF53098 (Ribonucle...)
    2. G3DSA:3.30.42...
  3. Domain IPR006133
    DNA-directed DNA polymerase, family B, exonuclease domain
    1. PF03104 (DNA_pol_B_...)
  4. Domain IPR023211
    DNA polymerase, palm domain
    1. G3DSA:3.90.16...
  5. Domain IPR006134
    DNA-directed DNA polymerase, family B, multifunctional domain
    1. PF00136 (DNA_pol_B)
  6. Domain IPR013697
    DNA polymerase epsilon, catalytic subunit A, C-terminal
    1. PF08490 (DUF1744)
    2. SM01159 (DUF1744_2)
Unintegrated signatures no IPR
Unintegrated signatures
  1. Coil
  2. G3DSA:3.30.34...
  3. SSF56672 (DNA/RNA p...)
  4. cd05535 (POLBc_epsilon)
  5. cd05779 (DNA_polB_e...)
  6. mobidb-lite (disord...)

Residue annotation

  1. catalytic site cd0...
  2. active site cd05779
  3. substrate binding ...
  4. active site cd05535
  5. metal-binding site...

Protein sequence

>MCA_06247_1
MSMKPPKNGQMRGYKPSSKPSISGAGIPMEATSISQRFESVHVTDKLNEKLGFPRYESGSKKVGWLVNMHSTTRQLDNSV
LNGNAGVDYYFLDEEGGGFSATVFYDPYFLIIGKKGYETEIEDYLKKALDGILKSTNRIQKEDLKLPNHLLGHKQMLIQL
NFHNVTDLLAARKLIMPLIQKNKEKKEIKDSYEEIALSNINNDLEDSSSNQRIIQDPSELIYDIREYDVPYHVRVAIDKD
IRVGKWYTVESKAGMIELTEMPEREIRADPVVLAFDIETTKLPLKFPDATIDKVMMISYMIDGEGFLITNREIVSQDIDD
FEYTPKPEYKGVFTIFNEPDEKSLLLRFFEHIQDAKPSVIATFNGDFFDWPFVEKRAAVNGIDMYEEIGFKIDSEAEYKS
AHCCHMDCFRWVKRDSYLPQGSQGLKAVTTAKLGYNPIEIDPELMTPYAIEKPQTMAEYSVSDAVATYYLYMKYVHPFIF
SLCNIIPLNPDEVLRKGTGTLCEMLLMVQAYKGSIVLPNKHTDPVERFYKGHLLETETYVGGHVESLEAGVFRSDIPSKF
EVDPTAVDEVLNDLDNALIFSIEVEAQKKLEDVTNYDEVKEAIAKELLELKNNPNRTEKPSIYHVDVASMYPNIMTTNRL
QPDSMITEEDCASCDFNRPGKTCDRRLPWAWRGEYFPAKMDEYLMIRRALENEVFPGKRPNAPPIPFQELSPTEKAAQIK
KRLTEYSRKVYHKIHQTETIEREAIICQRENPFYVNTVRDFRDRRYHFKTLQKVWKKKASEIDSSNAAAKEDAKKMIVLY
DSLQLAHKVILNSFYGYVMRKGSRWYSMEMAGVTCLTGATIIQMARSLVERLGRPLELDTDGIWCILPGSFPENFTFNLK
DGKKIFVSYPCVMLNHLVHARFTNHQYQTLVDPKTFKYSTMSDNSIFFEVDGPYKAMVLPTSKEEGKGLKKRYAVFNDDG
SLAELKGFELKRRGELKLIKTFQSQIFKEFLNGTTLEECYGAVARVANSWLDILESKGTTLDREELLDLISENRSMSKSL
EEYGDQKSTSITTAKRLAEFLGASMVKDRGLNCKYIISDRPYGAPVAERAIPVAIFSAEKSVKSHYMRKWLKDPSLDEFD
PRAILDWQYYWERLASTIQKIITIPAALQDVENPVPRIPHPDWLERKVNAKHDKFKQKKLSNFFQKASPKDDSAKIVDIE
DIGKSKDQLTAASIMPKVGKVSKVSKRTVASVDVEAPEEEFMTLPSEAPSIDGPYSEWLKYQKQVWKLQKQERARHKQVF
GQATAHRSTGIASMIQNQVEQAYSSENWSIVQVASSERPGEIKVTVSITGKLQTVKVKVPRKFYVNFKTGRRVPSFEEIQ
KQLPNCKIERAPTMVLPDGSTSTTLIRVTTSETIFIEKMNRVDSILRHPLVEGIYETSIDSVQNAVLELGICCRLDDSQP
GLLGKGLTSGFNLDWLIPVREDKNQRRSLPYLDSISFQYIHVVHISFSDYQVVNIISTHSNKAFTLVLRPTSQSQSLPNL
SRMYSSMLEEKLSKHGGKDPRQGHFITLPDQLEFEDFTFNNTYRFFGKVNKILETLQAERAIQTIAVLQSPTPERIKTMV
RAINDFPICQLREVDTNTLPAIAWQSLAGKKLLSQFFGLDKWLLHLHELSKYSQIPICNLTYDDMRYVMDVSYARKLRSN
GLVLWWSPKFMPDEGGKELDFTALAESEVAELPTVNNPGLHTSVCIDLDVRNLMINTVLTSSLIMNAEGTNSAINTTIVE
SNTRDGTVEEMVIPFVEDAFSTPAITALSSIVKTWWNEALREDEGFAATYSDSILSSFLSWVSSRGSFMYHKQLYQHVKT
LCKKVFIQLLREFRKVGSRIVFANQSRVVILTSKTSVETAYSYANYLVKSIKSKSFFHFLDINISQYWGRLIWMDEVNYG
GLRCKDIGADEESTKLQLVMNWDLCEFLPTKLSREFTKWVSAFIYAVDERLKAAMMDVDGEDGQESRPTQLPNSEQDDGE
ILKGVAKSLEKQLLARIKSLIQQQYMAQSVNLEEAEEQGNLEAIQLVDDFAVVDHPGSIADALESATAISKKNHSNNNIA
TSFGKAGSVSVSINNNMVLQLAKFLCAVFALAKPIELEVRLVRRHILALFEIREFSMDGNFTYPVSSLKIPNYTCTYCCE
VQDVDICRGVSRRLKQQRLRYSKNGGDNANSKLVFSLGTCQECGKELNRVELEELLIKQIYKMLTQYQVQDLKCVKCKRI
REDDMSEHCSCSGEWAETVPRSEILKRMSIFSHAAHFYGLKLLQELVDEIL

GO term prediction

Biological Process

GO:0006260 DNA replication

Molecular Function

GO:0000166 nucleotide binding
GO:0003676 nucleic acid binding
GO:0003677 DNA binding
GO:0003887 DNA-directed DNA polymerase activity
GO:0008270 zinc ion binding
GO:0008408 3'-5' exonuclease activity

Cellular Component

GO:0005634 nucleus