Protein

MIA_03086_1

Length
1,427 amino acids

Browser: contig04:203478-207808-

Protein function

EGGNOG:0PFVWPGUG_05887Translation Initiation Factor
SGD closest match:S000000033FUN12Eukaryotic translation initiation factor 5B
CGD closest match:CAL0000190309FUN12Translation initiation factor eIF5B

Protein alignments

%idAln lengthE-value
MCA_00542_188.034%5850.0MCA_00542_1
A0A0J9X6X1_GEOCN88.185%5840.0Similar to Saccharomyces cerevisiae YAL035W FUN12 GTPase required for general translation initiation OS=Geotrichum candidum GN=BN980_GECA04s02188g PE=4 SV=1
UniRef50_A0A1S8VSS749.203%10670.0Translation initiation factor aIF-2 n=1 Tax=Batrachochytrium salamandrivorans TaxID=1357716 RepID=A0A1S8VSS7_9FUNG
A0A060T2U6_BLAAD81.368%5850.0ARAD1A03454p OS=Blastobotrys adeninivorans GN=GNLVRS02_ARAD1A03454g PE=4 SV=1
A0A167D223_9ASCO80.513%5850.0Translation initiation factor eIF5B OS=Sugiyamaella lignohabitans GN=FUN12 PE=4 SV=1
Q6CI82_YARLI78.632%5850.0YALI0A00803p OS=Yarrowia lipolytica (strain CLIB 122 / E 150) GN=YALI0_A00803g PE=4 SV=1
Q5A782_CANAL79.316%5850.0Translation initiation factor eIF5B OS=Candida albicans (strain SC5314 / ATCC MYA-2876) GN=FUN12 PE=4 SV=1
A0A1E3PU19_9ASCO76.752%5850.0Uncharacterized protein OS=Nadsonia fulvescens var. elongata DSM 6958 GN=NADFUDRAFT_49072 PE=4 SV=1
IF2P_YEAST77.265%5850.0Eukaryotic translation initiation factor 5B OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=FUN12 PE=1 SV=2
A0A1E4TM30_9ASCO70.588%5780.0Uncharacterized protein OS=Tortispora caseinolytica NRRL Y-17796 GN=CANCADRAFT_93335 PE=4 SV=1

Mitochondrial localization by mitoprotII

Probability of mitochondrial location: 0.0099

Protein family membership

None predicted.

Domains and repeats

  1. Domain
  2. Domain
1 200 400 600 800 1000 1200 1427

Detailed signature matches

  1. Domain IPR007868
    Hom-end-associated Hint
    1. PF05203 (Hom_end_hint)
  2. Domain IPR028992
    Hedgehog/Intein (Hint) domain
    1. SSF51294 (Hedgehog/...)
  3. Domain IPR027417
    P-loop containing nucleoside triphosphate hydrolase
    1. SSF52540 (P-loop co...)
  4. Domain IPR000795
    Transcription factor, GTP-binding domain
    1. PS51722 (G_TR_2)
    2. PF00009 (GTP_EFTU)
  5. Domain IPR023115
    Translation initiation factor IF- 2, domain 3
    1. SSF52156 (Initiatio...)
    2. PF11987 (IF-2)
    3. G3DSA:3.40.50...
  6. Domain IPR009000
    Translation protein, beta-barrel domain
    1. SSF50447 (Translati...)
  7. Domain IPR004161
    Translation elongation factor EFTu-like, domain 2
    1. PF03144 (GTP_EFTU_D2)
Unintegrated signatures no IPR
Unintegrated signatures
  1. Coil
  2. G3DSA:2.170.1...
  3. G3DSA:2.40.30.10
  4. G3DSA:3.40.50...
  5. cd01887 (IF2_eIF5B)
  6. cd03703 (aeIF5B_II)
  7. mobidb-lite (disord...)

Residue annotation

  1. G1 box cd01887
  2. putative GEF inter...
  3. GTP/Mg2+ binding s...
  4. Switch I region cd...
  5. G2 box cd01887
  6. G3 box cd01887
  7. Switch II region c...
  8. G4 box cd01887
  9. G5 box cd01887
  10. 18S rRNA binding s...

Protein sequence

>MIA_03086_1
MAKKDKKGKKTAGYDWDDEEEAQPAAVEFGSEAPEPAAEPAEEPAGPQEPVAASADDMAEEFGGLMGALSKSKKKKVAKE
EAAAAEAKAAEESAVQIKSKKEKEREKKEREKQKKKQQAEQKKASLKAMNAALEESREKAKQEAERMRQEAIKNATSSDA
PATPAPAPKKKKAGGASAALQKRLIAQREEAEARKRAEDEEKARIAAEEARLAEEEKKKAEVRERKKQREREKREELRKA
GKLLTKKQKEQLRHAEIRRQQLLESGNIKVEALQGNQQTSRPTYEDKKKRPQTAEPAETKPAPELPIDEPVVAAVVTIKK
DEAKPEEEEEVLDSWENALDSDEEKEPETKESWDESSEEEEEPKAAPAPKTAEAEAAADEQSLEKALSHAQKNKELIEKK
YEERIKEAQSHANSKDLRSPICVILGHVDTGKCWGRDTPMLMFDGSLRPVQDVRAGDLLMGDDNTPRTVQPGSVIQGNGD
LFRIVPDAASGSDAFVCNGDHVLVLSIAQKPTVVESAGSFAIQSIAVDSKVPAINTVGSFATREQAEAALPEWKPLIWET
SLVEFVAFQKATPDLAALCSMYKPFDSVEYAQGNAFTEAVAKAFGVAPTLEQELDAAKLLGVSLLSSADLTCDKAVATLA
ESLGMLKSKQIPAAIVSASAEHRKAFLSGIVDGSALSGGAPVEHTLAHVDNAQVAQAANELFPDSASKILGSEKLVSGIA
GEVIFDAASSLRKHDAHLTHAWEIASDKPELLVQVRRLARSIGLRAGAVSSTLTISGPAMSSLNKYLTVEANKVAANSAL
YDQVVSSSWGFGIEELGNGDYFGFTLDGNSRLLLGDFTVSHNTKLLDKIRQTNVQEGEAGGITQQIGATYFPISAIEKKT
EVMKKYVKVEYNVPGLLVIDTPGHESFSNLRSRGSSLCNIAILVIDIMHGLEQQTIESIKLLRDRKTPFVVALNKIDRLY
GWESIPNNSFRDSFSKQASSVQREFQDRYEKAKLALAEQGLNSELYFQNKNVARNVSIIPTSAVTGEGVPDLLYLLLELT
QTRMSKQLMYLSTLEATILEVKVVEGLGTTIDVVLSNGVLHEGDRIVLCGQNGPIATNVRALLTPQPLRELRIKSAYVHH
KEVKAALGVKISAPDLEKAVAGSRLLLVGPDDDEEELMDEVMDDLTDLLDSVDKTGLGVCVQASTLGALEALLDFLKDMK
IPVMSIGIGPVYKRDVMRATTMLEKKPELALMLCFDVKIDKDAEQYAKEQNIKIFNADIIYHLFDSFTAYQKQLLEKKRE
DNASLAVYPCVCKTIQIINKRNPMIIGLEVTEGSIRTGTPITVVKKDPESGAKLILDLGRIVSMEVNHKSVDVVKKGQAN
NGVAVRLSDPTSSAQPIWGRHVDEKDTLYSHISRKSIDVLKDPAFRDSVPRDDWVLIKNLKPLFDIK

GO term prediction

Biological Process

GO:0030908 protein splicing

Molecular Function

GO:0003924 GTPase activity
GO:0005525 GTP binding

Cellular Component

None predicted.