Protein

MCA_00542_1

Length
1,037 amino acids

Gene name: FUN12

Description: Translation initiation factor 5B

Browser: contigA:1689867-1693054-

RNA-seq: read pairs 8624, FPKM 102.7, percentile rank 79.1% (100% = highest expression)

Protein function

Annotation:FUN12Translation initiation factor 5B
KEGG:K03243EIF5B translation initiation factor 5B
EGGNOG:0PFVWPGUG_05887Translation Initiation Factor
SGD closest match:S000000033FUN12Eukaryotic translation initiation factor 5B
CGD closest match:CAL0000190309FUN12Translation initiation factor eIF5B

Protein alignments

%idAln lengthE-value
A0A0J9X6X1_GEOCN75.95%7900.0Similar to Saccharomyces cerevisiae YAL035W FUN12 GTPase required for general translation initiation OS=Geotrichum candidum GN=BN980_GECA04s02188g PE=4 SV=1
MIA_03086_188.03%5850.0MIA_03086_1
A0A060T2U6_BLAAD80.16%6350.0ARAD1A03454p OS=Blastobotrys adeninivorans GN=GNLVRS02_ARAD1A03454g PE=4 SV=1
A0A167D223_9ASCO78.02%6370.0Translation initiation factor eIF5B OS=Sugiyamaella lignohabitans GN=FUN12 PE=4 SV=1
UniRef50_A0A167D22378.02%6370.0Translation initiation factor eIF5B n=208 Tax=Fungi TaxID=4751 RepID=A0A167D223_9ASCO
Q6CI82_YARLI76.87%6270.0YALI0A00803p OS=Yarrowia lipolytica (strain CLIB 122 / E 150) GN=YALI0_A00803g PE=4 SV=1
Q5A782_CANAL76.30%6330.0Translation initiation factor eIF5B OS=Candida albicans (strain SC5314 / ATCC MYA-2876) GN=FUN12 PE=4 SV=1
A0A1E3PU19_9ASCO71.27%7030.0Uncharacterized protein OS=Nadsonia fulvescens var. elongata DSM 6958 GN=NADFUDRAFT_49072 PE=4 SV=1
IF2P_YEAST77.85%6050.0Eukaryotic translation initiation factor 5B OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=FUN12 PE=1 SV=2
A0A1E4TM30_9ASCO69.59%6380.0Uncharacterized protein OS=Tortispora caseinolytica NRRL Y-17796 GN=CANCADRAFT_93335 PE=4 SV=1

Mitochondrial localization by mitoprotII

Probability of mitochondrial location: 0.0011

Protein family membership

None predicted.

Domains and repeats

  1. Domain
  2. Domain
1 100 200 300 400 500 600 700 800 900 1037

Detailed signature matches

  1. Domain IPR027417
    P-loop containing nucleoside triphosphate hydrolase
    1. SSF52540 (P-loop co...)
  2. Domain IPR005225
    Small GTP-binding protein domain
    1. TIGR00231
  3. Domain IPR000795
    Transcription factor, GTP-binding domain
    1. PS51722 (G_TR_2)
    2. PF00009 (GTP_EFTU)
    3. PR00315 (ELONGATNFCT)
  4. Domain IPR004161
    Translation elongation factor EFTu-like, domain 2
    1. PF03144 (GTP_EFTU_D2)
  5. Domain IPR023115
    Translation initiation factor IF- 2, domain 3
    1. SSF52156 (Initiatio...)
    2. PF11987 (IF-2)
    3. G3DSA:3.40.50...
  6. Domain IPR009000
    Translation protein, beta-barrel domain
    1. SSF50447 (Translati...)
Unintegrated signatures no IPR
Unintegrated signatures
  1. Coil
  2. G3DSA:2.40.30.10
  3. G3DSA:3.40.50...
  4. cd01887 (IF2_eIF5B)
  5. cd03703 (aeIF5B_II)
  6. mobidb-lite (disord...)

Residue annotation

  1. G1 box cd01887
  2. putative GEF inter...
  3. GTP/Mg2+ binding s...
  4. Switch I region cd...
  5. G2 box cd01887
  6. G3 box cd01887
  7. Switch II region c...
  8. G4 box cd01887
  9. G5 box cd01887
  10. 18S rRNA binding s...

Protein sequence

>MCA_00542_1
MAKKDKKGKKGGWIDDDEDDEVMDTAPIEFGAESTPEPAAEPPTEAPNDDVEQVTEEIGGLMGALNKSKKKKVAKEEAKA
AEAKAAEESAVQVKSKKEKEREKKEREKLKKKQQAEARKANQKALNAALEEERERAKKMAQALKEEELSKGESQPKAAPA
ASKKKKAGGVSAALQKRLIAQREAADAQRKAEEEEKKRLAEEAARLAEEERIKEEARQKKKERERLKREELRKQGKLLTK
KQKEQQKMAEIRRKQLLESGNIVVEALQGDVSTKKSKPVYEDKKPREKPASAEKKETSKPSTPVSEPISEPVVAAVVEIK
KEEPKEEKAEAEDEPVLESWEDALDSDGEVKDNWEELADESDKEDDDSDKKQAEEEEKEEALEKTPSKSTESTESTLVQP
NELEKAVSEAHKKKDTIEKKYEERIKGAMEKANNKDLRSPICCILGHVDTGKTKLLDKIRQTNVQEGEAGGITQQIGATY
FPIDAIEKKTEVMKKYVNIEYSVPGLLIIDTPGHESFSNLRSRGSSLCNIAILVIDIMHGLEQQTIESIKLLRDRKTPFI
VALNKIDRCYGWEAVPNNSFRDSLARQSSTVQREFQDRYEKAKLALAEQGLNSELYYQNKNVARNVSIVPTSAVTGEGVP
DLLYLLLELTQSRMSKQLMYLSTLEATVLEVKVVEGLGVTIDVVLSNGILREGDRIVLCGQNGPIVTTVRALLTPQPLRE
LRIKSAYVHHKEVKAALGVKIAANDLDKAVAGSRLLLVGPDDDEEELMDEVMDDLTGLLDSVDKTGMGVCVQASTLGSLE
ALLDFLKEMKIPVMSIGIGPVFKRDVMRATTMLEKKPELAIMLCFDVKIDKEAEQYAQENNIRIFNSNIIYHLFDSFTAY
QQQLLEKKRQDNADLAIYPCILKTIQIINKRNPMIIGVDIISGSLRIGTPIAVVKSDPATGAKMILDLGRVVSMEVNHKS
VDIVKKGQAAAGVAMRLSDPTGSSQPTWGRHVDEKDTLYSHITRKSIDVLKDPAFRDGVPREDWLLIKQLKPYFDIK

GO term prediction

Biological Process

None predicted.

Molecular Function

GO:0003924 GTPase activity
GO:0005525 GTP binding

Cellular Component

None predicted.