MIA_02927_1

Protein

Length

1,223 amino acids

Browser: contig03:2098485-2102208+

Protein function

EGGNOG:	0PIB0	PAN2	Catalytic subunit of the poly(A)-nuclease (PAN) deadenylation complex involved in cytoplasmic mRNA turnover. PAN specifically shortens poly(A) tails of RNA when the poly(A) stretch is bound by polyadenylate-binding protein PAB1. Deadenylation of the 3'-tail to a length that is too short to bind PAB1 induces mRNA decay. May also be involved in post- transcriptional maturation of mRNA poly(A) tails (By similarity)
SGD closest match:	S000003062	PAN2	PAB-dependent poly(A)-specific ribonuclease subunit PAN2
CGD closest match:	CAL0000194881	PAN2	PAB-dependent poly(A)-specific ribonuclease subunit PAN2

Protein alignments

	%id	Aln length	E-value
MCA_00852_1	50.469%	747	0.0	MCA_00852_1
UniRef50_A1CEK7	31.908%	1263	4.25e-171	PAB-dependent poly(A)-specific ribonuclease subunit pan2 n=66 Tax=leotiomyceta TaxID=716546 RepID=PAN2_ASPCL
A0A0J9X5Y0_GEOCN	48.799%	541	6.51e-145	PAB-dependent poly(A)-specific ribonuclease subunit PAN2 OS=Geotrichum candidum GN=PAN2 PE=3 SV=1
A0A167EV86_9ASCO	47.091%	550	3.46e-139	Pan2p OS=Sugiyamaella lignohabitans GN=PAN2 PE=4 SV=1
A0A060T903_BLAAD	46.692%	529	4.80e-137	PAB-dependent poly(A)-specific ribonuclease subunit PAN2 OS=Blastobotrys adeninivorans GN=PAN2 PE=3 SV=1
A0A1E3PRD9_9ASCO	39.459%	555	7.73e-111	PAB-dependent poly(A)-specific ribonuclease subunit PAN2 OS=Nadsonia fulvescens var. elongata DSM 6958 GN=PAN2 PE=3 SV=1
PAN2_YARLI	39.250%	507	3.48e-102	PAB-dependent poly(A)-specific ribonuclease subunit PAN2 OS=Yarrowia lipolytica (strain CLIB 122 / E 150) GN=PAN2 PE=3 SV=2
PAN2_CANAL	42.334%	437	6.72e-91	PAB-dependent poly(A)-specific ribonuclease subunit PAN2 OS=Candida albicans (strain SC5314 / ATCC MYA-2876) GN=PAN2 PE=3 SV=2
PAN2_YEAST	46.130%	323	7.75e-78	PAB-dependent poly(A)-specific ribonuclease subunit PAN2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=PAN2 PE=1 SV=1
A0A1E4TAQ6_9ASCO	31.778%	450	1.27e-50	Uncharacterized protein (Fragment) OS=Tortispora caseinolytica NRRL Y-17796 GN=CANCADRAFT_32279 PE=4 SV=1

Mitochondrial localization by mitoprotII

Probability of mitochondrial location: 0.1468

Protein family membership

PAB-dependent poly(A)-specific ribonuclease subunit PAN2 (IPR030843)

Domains and repeats

Domain
2 - 364
WD40/YVTN repeat-like-containing domain (IPR015943)
WD40-repeat-containing domain (IPR017986)
563 - 954
Ubiquitin specific protease domain (IPR028889)
PAN2 domain (IPR028881)
1,003 - 1,196
Ribonuclease H-like domain (IPR012337)
Exonuclease, RNase T/DNA polymerase III (IPR013520)

1 200 400 600 800 1000 1223

Detailed signature matches

IPR030843

PAB-dependent poly(A)-specific ribonuclease subunit PAN2
1. MF_03182 (PAN2)
  
  1 - 1,221
  
  HAMAP
  MF_03182 (PAN2)
IPR015943

WD40/YVTN repeat-like-containing domain
1. G3DSA:2.130.1...
  
  2 - 364
  
  GENE3D
  G3DSA:2.130.10.10 (G3DSA:2.130.10.10)
IPR017986

WD40-repeat-containing domain
1. SSF50978 (WD40 repe...)
  
  160 - 333
  
  SUPERFAMILY
  SSF50978 (WD40 repeat-like)
  
  12 - 59
  
  SUPERFAMILY
  SSF50978 (WD40 repeat-like)
IPR028889

Ubiquitin specific protease domain
1. PS50235 (USP_3)
  
  563 - 954
  
  PROSITE profiles
  PS50235 (USP_3)
IPR028881

PAN2 domain
1. PF13423 (UCH_1)
  
  563 - 881
  
  Pfam
  PF13423 (UCH_1)
IPR012337

Ribonuclease H-like domain
1. SSF53098 (Ribonucle...)
  
  1,007 - 1,191
  
  SUPERFAMILY
  SSF53098 (Ribonuclease H-like)
2. G3DSA:3.30.42...
  
  1,003 - 1,196
  
  GENE3D
  G3DSA:3.30.420.10 (G3DSA:3.30.420.10)
IPR013520

Exonuclease, RNase T/DNA polymerase III
1. PF00929 (RNase_T)
  
  1,012 - 1,186
  
  Pfam
  PF00929 (RNase_T)
2. SM00479 (exoiiiendus)
  
  1,010 - 1,195
  
  SMART
  SM00479 (exoiiiendus)

no IPR

Unintegrated signatures

G3DSA:3.90.70.10

556 - 956

GENE3D
G3DSA:3.90.70.10 (G3DSA:3.90.70.10)
SSF54001 (Cysteine ...)

559 - 659

SUPERFAMILY
SSF54001 (Cysteine proteinases)

866 - 978

SUPERFAMILY
SSF54001 (Cysteine proteinases)

736 - 832

SUPERFAMILY
SSF54001 (Cysteine proteinases)
cd06143 (PAN2_exo)

1,012 - 1,187

CDD
cd06143 (PAN2_exo)
mobidb-lite (disord...)

884 - 907

MobiDB Lite
mobidb-lite (disorder_prediction)

Residue annotation

catalytic site cd0...

1,015D

catalytic site
1,015D, 1,017E, 1,125D, 1,173H, 1,178D
CDD
cd06143 (PAN2_exo)

1,017E

catalytic site
1,015D, 1,017E, 1,125D, 1,173H, 1,178D
CDD
cd06143 (PAN2_exo)

1,125D

catalytic site
1,015D, 1,017E, 1,125D, 1,173H, 1,178D
CDD
cd06143 (PAN2_exo)

1,173H

catalytic site
1,015D, 1,017E, 1,125D, 1,173H, 1,178D
CDD
cd06143 (PAN2_exo)

1,178D

catalytic site
1,015D, 1,017E, 1,125D, 1,173H, 1,178D
CDD
cd06143 (PAN2_exo)
active site cd06143

1,015D

active site
1,015D, 1,016T, 1,017E, 1,018F, 1,020L, 1,076L, 1,080S, 1,120H, 1,121G, 1,123D, 1,124N, 1,125D, 1,173H, 1,178D
CDD
cd06143 (PAN2_exo)

1,016T

active site
1,015D, 1,016T, 1,017E, 1,018F, 1,020L, 1,076L, 1,080S, 1,120H, 1,121G, 1,123D, 1,124N, 1,125D, 1,173H, 1,178D
CDD
cd06143 (PAN2_exo)

1,017E

active site
1,015D, 1,016T, 1,017E, 1,018F, 1,020L, 1,076L, 1,080S, 1,120H, 1,121G, 1,123D, 1,124N, 1,125D, 1,173H, 1,178D
CDD
cd06143 (PAN2_exo)

1,018F

active site
1,015D, 1,016T, 1,017E, 1,018F, 1,020L, 1,076L, 1,080S, 1,120H, 1,121G, 1,123D, 1,124N, 1,125D, 1,173H, 1,178D
CDD
cd06143 (PAN2_exo)

1,020L

active site
1,015D, 1,016T, 1,017E, 1,018F, 1,020L, 1,076L, 1,080S, 1,120H, 1,121G, 1,123D, 1,124N, 1,125D, 1,173H, 1,178D
CDD
cd06143 (PAN2_exo)

1,076L

active site
1,015D, 1,016T, 1,017E, 1,018F, 1,020L, 1,076L, 1,080S, 1,120H, 1,121G, 1,123D, 1,124N, 1,125D, 1,173H, 1,178D
CDD
cd06143 (PAN2_exo)

1,080S

active site
1,015D, 1,016T, 1,017E, 1,018F, 1,020L, 1,076L, 1,080S, 1,120H, 1,121G, 1,123D, 1,124N, 1,125D, 1,173H, 1,178D
CDD
cd06143 (PAN2_exo)

1,120H

active site
1,015D, 1,016T, 1,017E, 1,018F, 1,020L, 1,076L, 1,080S, 1,120H, 1,121G, 1,123D, 1,124N, 1,125D, 1,173H, 1,178D
CDD
cd06143 (PAN2_exo)

1,121G

active site
1,015D, 1,016T, 1,017E, 1,018F, 1,020L, 1,076L, 1,080S, 1,120H, 1,121G, 1,123D, 1,124N, 1,125D, 1,173H, 1,178D
CDD
cd06143 (PAN2_exo)

1,123D

active site
1,015D, 1,016T, 1,017E, 1,018F, 1,020L, 1,076L, 1,080S, 1,120H, 1,121G, 1,123D, 1,124N, 1,125D, 1,173H, 1,178D
CDD
cd06143 (PAN2_exo)

1,124N

active site
1,015D, 1,016T, 1,017E, 1,018F, 1,020L, 1,076L, 1,080S, 1,120H, 1,121G, 1,123D, 1,124N, 1,125D, 1,173H, 1,178D
CDD
cd06143 (PAN2_exo)

1,125D

active site
1,015D, 1,016T, 1,017E, 1,018F, 1,020L, 1,076L, 1,080S, 1,120H, 1,121G, 1,123D, 1,124N, 1,125D, 1,173H, 1,178D
CDD
cd06143 (PAN2_exo)

1,173H

active site
1,015D, 1,016T, 1,017E, 1,018F, 1,020L, 1,076L, 1,080S, 1,120H, 1,121G, 1,123D, 1,124N, 1,125D, 1,173H, 1,178D
CDD
cd06143 (PAN2_exo)

1,178D

active site
1,015D, 1,016T, 1,017E, 1,018F, 1,020L, 1,076L, 1,080S, 1,120H, 1,121G, 1,123D, 1,124N, 1,125D, 1,173H, 1,178D
CDD
cd06143 (PAN2_exo)
substrate binding ...

1,015D

substrate binding site
1,015D, 1,016T, 1,017E, 1,018F, 1,020L, 1,076L, 1,080S, 1,120H, 1,121G, 1,123D, 1,124N, 1,173H, 1,178D
CDD
cd06143 (PAN2_exo)

1,016T

substrate binding site
1,015D, 1,016T, 1,017E, 1,018F, 1,020L, 1,076L, 1,080S, 1,120H, 1,121G, 1,123D, 1,124N, 1,173H, 1,178D
CDD
cd06143 (PAN2_exo)

1,017E

substrate binding site
1,015D, 1,016T, 1,017E, 1,018F, 1,020L, 1,076L, 1,080S, 1,120H, 1,121G, 1,123D, 1,124N, 1,173H, 1,178D
CDD
cd06143 (PAN2_exo)

1,018F

substrate binding site
1,015D, 1,016T, 1,017E, 1,018F, 1,020L, 1,076L, 1,080S, 1,120H, 1,121G, 1,123D, 1,124N, 1,173H, 1,178D
CDD
cd06143 (PAN2_exo)

1,020L

substrate binding site
1,015D, 1,016T, 1,017E, 1,018F, 1,020L, 1,076L, 1,080S, 1,120H, 1,121G, 1,123D, 1,124N, 1,173H, 1,178D
CDD
cd06143 (PAN2_exo)

1,076L

substrate binding site
1,015D, 1,016T, 1,017E, 1,018F, 1,020L, 1,076L, 1,080S, 1,120H, 1,121G, 1,123D, 1,124N, 1,173H, 1,178D
CDD
cd06143 (PAN2_exo)

1,080S

substrate binding site
1,015D, 1,016T, 1,017E, 1,018F, 1,020L, 1,076L, 1,080S, 1,120H, 1,121G, 1,123D, 1,124N, 1,173H, 1,178D
CDD
cd06143 (PAN2_exo)

1,120H

substrate binding site
1,015D, 1,016T, 1,017E, 1,018F, 1,020L, 1,076L, 1,080S, 1,120H, 1,121G, 1,123D, 1,124N, 1,173H, 1,178D
CDD
cd06143 (PAN2_exo)

1,121G

substrate binding site
1,015D, 1,016T, 1,017E, 1,018F, 1,020L, 1,076L, 1,080S, 1,120H, 1,121G, 1,123D, 1,124N, 1,173H, 1,178D
CDD
cd06143 (PAN2_exo)

1,123D

substrate binding site
1,015D, 1,016T, 1,017E, 1,018F, 1,020L, 1,076L, 1,080S, 1,120H, 1,121G, 1,123D, 1,124N, 1,173H, 1,178D
CDD
cd06143 (PAN2_exo)

1,124N

substrate binding site
1,015D, 1,016T, 1,017E, 1,018F, 1,020L, 1,076L, 1,080S, 1,120H, 1,121G, 1,123D, 1,124N, 1,173H, 1,178D
CDD
cd06143 (PAN2_exo)

1,173H

substrate binding site
1,015D, 1,016T, 1,017E, 1,018F, 1,020L, 1,076L, 1,080S, 1,120H, 1,121G, 1,123D, 1,124N, 1,173H, 1,178D
CDD
cd06143 (PAN2_exo)

1,178D

substrate binding site
1,015D, 1,016T, 1,017E, 1,018F, 1,020L, 1,076L, 1,080S, 1,120H, 1,121G, 1,123D, 1,124N, 1,173H, 1,178D
CDD
cd06143 (PAN2_exo)

Protein sequence

>MIA_02927_1
MDDWNELTSLPVFSRPYASVSATFFDPAQDLLWAGASDGFLASFYTQALRPYASTRVHASLPVLQIAAADKALLALTPSS
LRLSSRRATAKATLDAPASLAGSLTCMAPARDSDLYIGVGSSSATALPGAAATNEILKLNLDAPAASVFASASKIPLSPG
SAGAVKIIRTAHALVAGKSNGAVDIIDPNSNRVVKSFQAHTSIISDLDARETTLLTCGYSLRKTGPAGQLLLDPLVNIFD
LRAGKPLPPVPFPAGAGFVRLHPKLSTCAFIASQTGQIQLIDFATPASTQVRLHQAALPSSGTLSGLEVSSSGDFLALTD
GAYVQLWSNSADDPAFNEFAAPLEFPIDPAVYTHPISSMIPGQPRRIDIDDYSTPLSAIGMPYYKDELLSSWSIEPGMPM
VFDQGMPSPKLPPDVLRQLQAAINDNASTEIHPTDPVLGGIGLTGISAHNHARRNVAQKYVSFKDLKRRTVAAGPKFISE
RGGINAAAAAAAAAAAASNPLVSTTNLLNESIQTFSSTMAENDTSTKEEQSPVPPYYRRLEIKYSKFGIADFDFGYYNHT
KYSGLEPQFVNSYCNPLLQLYCYSQPIYEFALNQLKHNSLDDKSLLAELGFLFDMLHKARGLHCAAANFVKALATIPQAN
ALGLVTDDAHPFRLGSRSLSVSSTAATTTQPLPPAASSLAAICSRTLSMATGSASGYVSGHGHGHDHGQGLLLQAFARFL
MERMAIDERAYDSSAREFEKIAGIVVETSTHSPACQSHSVLETIVYSLELVPPKAPFLQTLQASLDKRSRSSKAWCDSCR
KFHPQTATRSVRARLPKVLNLHVPLTEEPLPAGTWPDPVFAVRTETVAANDSSNTCLKIVAQPEAGDDIYELLGLVVEIQ
TDASADSTAPASASSATSGPRPSRNPADTHLISFVKIEGQWHLFNDFLVKPVSEAEALQVTDWRSPVVLLYQQKEPPSDA
PPEPFDYDAWRNSFDTSILYHDHFAAGFRAGLKRDYELLDPACEAPTPGTLVAMDTEFVLLQHEETEIISDGTKSLVRPK
QVSLARVSVVRGAGPKQGVPFIDDFIATSETIVDYLTEYSGVEQGDLDPWTSTRASLVTQQTSYKRLWLLLNLGCVFVGH
GLDNDFRTIGIHVPAAQVVDTLELFYLPEYKRRLSLKFLAYALLHENVQTGNHDSIEDAVTALRLYKEYLRVSSKGPDAF
VALLHQLYKEGKKCNFRPPPKEV

GO term prediction

Biological Process

GO:0000289 nuclear-transcribed mRNA poly(A) tail shortening
GO:0006397 mRNA processing

Molecular Function

GO:0003676 nucleic acid binding
GO:0004535 poly(A)-specific ribonuclease activity
GO:0005515 protein binding

Cellular Component

GO:0031251 PAN complex

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Entry type

Status

Per-residue features