MCA_00852_1

Protein

Length

1,642 amino acids

Gene name: PAN2

Description: PAB-dependent poly(A)-specific ribonuclease subunit PAN2

Browser: contigA:2667481-2672542+

RNA-seq: read pairs 2273, FPKM 17.1, percentile rank 37.1% (100% = highest expression)

Protein function

Annotation:		PAN2	PAB-dependent poly(A)-specific ribonuclease subunit PAN2
KEGG:	K12571	PAN2	PAB-dependent poly(A)-specific ribonuclease subunit 2 [EC:3.1.13.4]
EGGNOG:	0PIB0	PAN2	Catalytic subunit of the poly(A)-nuclease (PAN) deadenylation complex involved in cytoplasmic mRNA turnover. PAN specifically shortens poly(A) tails of RNA when the poly(A) stretch is bound by polyadenylate-binding protein PAB1. Deadenylation of the 3'-tail to a length that is too short to bind PAB1 induces mRNA decay. May also be involved in post- transcriptional maturation of mRNA poly(A) tails (By similarity)
SGD closest match:	S000003062	PAN2	PAB-dependent poly(A)-specific ribonuclease subunit PAN2
CGD closest match:	CAL0000194881	PAN2	PAB-dependent poly(A)-specific ribonuclease subunit PAN2

Protein alignments

	%id	Aln length	E-value
MIA_02927_1	50.20%	749	0.0	MIA_02927_1
A0A167EV86_9ASCO	66.56%	302	4e-121	Pan2p OS=Sugiyamaella lignohabitans GN=PAN2 PE=4 SV=1
UniRef50_A0A167EV86	66.56%	302	1e-117	Pan2p n=1 Tax=Sugiyamaella lignohabitans TaxID=796027 RepID=A0A167EV86_9ASCO
A0A0J9X5Y0_GEOCN	66.45%	301	7e-120	PAB-dependent poly(A)-specific ribonuclease subunit PAN2 OS=Geotrichum candidum GN=PAN2 PE=3 SV=1
A0A060T903_BLAAD	65.88%	296	2e-117	PAB-dependent poly(A)-specific ribonuclease subunit PAN2 OS=Blastobotrys adeninivorans GN=PAN2 PE=3 SV=1
A0A1E3PRD9_9ASCO	52.85%	333	2e-103	PAB-dependent poly(A)-specific ribonuclease subunit PAN2 OS=Nadsonia fulvescens var. elongata DSM 6958 GN=PAN2 PE=3 SV=1
PAN2_CANAL	52.44%	307	3e-89	PAB-dependent poly(A)-specific ribonuclease subunit PAN2 OS=Candida albicans (strain SC5314 / ATCC MYA-2876) GN=PAN2 PE=3 SV=2
PAN2_YARLI	47.35%	340	1e-81	PAB-dependent poly(A)-specific ribonuclease subunit PAN2 OS=Yarrowia lipolytica (strain CLIB 122 / E 150) GN=PAN2 PE=3 SV=2
PAN2_YEAST	48.50%	301	6e-80	PAB-dependent poly(A)-specific ribonuclease subunit PAN2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=PAN2 PE=1 SV=1
A0A1E4TAQ6_9ASCO	46.61%	221	5e-50	Uncharacterized protein (Fragment) OS=Tortispora caseinolytica NRRL Y-17796 GN=CANCADRAFT_32279 PE=4 SV=1

Mitochondrial localization by mitoprotII

Probability of mitochondrial location: 0.0642

Protein family membership

PAB-dependent poly(A)-specific ribonuclease subunit PAN2 (IPR030843)

Domains and repeats

Domain
13 - 372
WD40/YVTN repeat-like-containing domain (IPR015943)
WD40-repeat-containing domain (IPR017986)
646 - 775
PAN2 domain (IPR028881)
849 - 1,098
PAN2 domain (IPR028881)
1,402 - 1,596
Ribonuclease H-like domain (IPR012337)
Exonuclease, RNase T/DNA polymerase III (IPR013520)

1 200 400 600 800 1000 1200 1400 1642

Detailed signature matches

IPR030843

PAB-dependent poly(A)-specific ribonuclease subunit PAN2
1. MF_03182 (PAN2)
  
  1 - 1,622
  
  HAMAP
  MF_03182 (PAN2)
IPR015943

WD40/YVTN repeat-like-containing domain
1. G3DSA:2.130.1...
  
  13 - 372
  
  GENE3D
  G3DSA:2.130.10.10 (G3DSA:2.130.10.10)
IPR017986

WD40-repeat-containing domain
1. SSF50978 (WD40 repe...)
  
  18 - 82
  
  SUPERFAMILY
  SSF50978 (WD40 repeat-like)
  
  172 - 335
  
  SUPERFAMILY
  SSF50978 (WD40 repeat-like)
IPR028881

PAN2 domain
1. PF13423 (UCH_1)
  
  646 - 775
  
  Pfam
  PF13423 (UCH_1)
  
  849 - 1,098
  
  Pfam
  PF13423 (UCH_1)
IPR012337

Ribonuclease H-like domain
1. SSF53098 (Ribonucle...)
  
  1,412 - 1,595
  
  SUPERFAMILY
  SSF53098 (Ribonuclease H-like)
2. G3DSA:3.30.42...
  
  1,402 - 1,595
  
  GENE3D
  G3DSA:3.30.420.10 (G3DSA:3.30.420.10)
IPR013520

Exonuclease, RNase T/DNA polymerase III
1. PF00929 (RNase_T)
  
  1,413 - 1,587
  
  Pfam
  PF00929 (RNase_T)
2. SM00479 (exoiiiendus)
  
  1,411 - 1,596
  
  SMART
  SM00479 (exoiiiendus)

no IPR

Unintegrated signatures

Coil

1,640 - 1,642

COILS
Coil (Coil)
G3DSA:3.90.70.10

639 - 1,359

GENE3D
G3DSA:3.90.70.10 (G3DSA:3.90.70.10)
SSF54001 (Cysteine ...)

916 - 1,057

SUPERFAMILY
SSF54001 (Cysteine proteinases)

644 - 772

SUPERFAMILY
SSF54001 (Cysteine proteinases)

1,318 - 1,356

SUPERFAMILY
SSF54001 (Cysteine proteinases)

1,234 - 1,258

SUPERFAMILY
SSF54001 (Cysteine proteinases)
cd02257 (Peptidase_C19)

1,318 - 1,356

CDD
cd02257 (Peptidase_C19)
cd06143 (PAN2_exo)

1,413 - 1,588

CDD
cd06143 (PAN2_exo)
mobidb-lite (disord...)

1,249 - 1,281

MobiDB Lite
mobidb-lite (disorder_prediction)

479 - 509

MobiDB Lite
mobidb-lite (disorder_prediction)

539 - 572

MobiDB Lite
mobidb-lite (disorder_prediction)

1,049 - 1,144

MobiDB Lite
mobidb-lite (disorder_prediction)

971 - 991

MobiDB Lite
mobidb-lite (disorder_prediction)

Residue annotation

Active Site cd02257

1,329D

Active Site
1,329D
CDD
cd02257 (Peptidase_C19)
catalytic site cd0...

1,416D

catalytic site
1,416D, 1,418E, 1,526D, 1,574H, 1,579D
CDD
cd06143 (PAN2_exo)

1,418E

catalytic site
1,416D, 1,418E, 1,526D, 1,574H, 1,579D
CDD
cd06143 (PAN2_exo)

1,526D

catalytic site
1,416D, 1,418E, 1,526D, 1,574H, 1,579D
CDD
cd06143 (PAN2_exo)

1,574H

catalytic site
1,416D, 1,418E, 1,526D, 1,574H, 1,579D
CDD
cd06143 (PAN2_exo)

1,579D

catalytic site
1,416D, 1,418E, 1,526D, 1,574H, 1,579D
CDD
cd06143 (PAN2_exo)
substrate binding ...

1,416D

substrate binding site
1,416D, 1,417T, 1,418E, 1,419F, 1,421L, 1,477L, 1,481S, 1,521H, 1,522G, 1,524Q, 1,525N, 1,574H, 1,579D
CDD
cd06143 (PAN2_exo)

1,417T

substrate binding site
1,416D, 1,417T, 1,418E, 1,419F, 1,421L, 1,477L, 1,481S, 1,521H, 1,522G, 1,524Q, 1,525N, 1,574H, 1,579D
CDD
cd06143 (PAN2_exo)

1,418E

substrate binding site
1,416D, 1,417T, 1,418E, 1,419F, 1,421L, 1,477L, 1,481S, 1,521H, 1,522G, 1,524Q, 1,525N, 1,574H, 1,579D
CDD
cd06143 (PAN2_exo)

1,419F

substrate binding site
1,416D, 1,417T, 1,418E, 1,419F, 1,421L, 1,477L, 1,481S, 1,521H, 1,522G, 1,524Q, 1,525N, 1,574H, 1,579D
CDD
cd06143 (PAN2_exo)

1,421L

substrate binding site
1,416D, 1,417T, 1,418E, 1,419F, 1,421L, 1,477L, 1,481S, 1,521H, 1,522G, 1,524Q, 1,525N, 1,574H, 1,579D
CDD
cd06143 (PAN2_exo)

1,477L

substrate binding site
1,416D, 1,417T, 1,418E, 1,419F, 1,421L, 1,477L, 1,481S, 1,521H, 1,522G, 1,524Q, 1,525N, 1,574H, 1,579D
CDD
cd06143 (PAN2_exo)

1,481S

substrate binding site
1,416D, 1,417T, 1,418E, 1,419F, 1,421L, 1,477L, 1,481S, 1,521H, 1,522G, 1,524Q, 1,525N, 1,574H, 1,579D
CDD
cd06143 (PAN2_exo)

1,521H

substrate binding site
1,416D, 1,417T, 1,418E, 1,419F, 1,421L, 1,477L, 1,481S, 1,521H, 1,522G, 1,524Q, 1,525N, 1,574H, 1,579D
CDD
cd06143 (PAN2_exo)

1,522G

substrate binding site
1,416D, 1,417T, 1,418E, 1,419F, 1,421L, 1,477L, 1,481S, 1,521H, 1,522G, 1,524Q, 1,525N, 1,574H, 1,579D
CDD
cd06143 (PAN2_exo)

1,524Q

substrate binding site
1,416D, 1,417T, 1,418E, 1,419F, 1,421L, 1,477L, 1,481S, 1,521H, 1,522G, 1,524Q, 1,525N, 1,574H, 1,579D
CDD
cd06143 (PAN2_exo)

1,525N

substrate binding site
1,416D, 1,417T, 1,418E, 1,419F, 1,421L, 1,477L, 1,481S, 1,521H, 1,522G, 1,524Q, 1,525N, 1,574H, 1,579D
CDD
cd06143 (PAN2_exo)

1,574H

substrate binding site
1,416D, 1,417T, 1,418E, 1,419F, 1,421L, 1,477L, 1,481S, 1,521H, 1,522G, 1,524Q, 1,525N, 1,574H, 1,579D
CDD
cd06143 (PAN2_exo)

1,579D

substrate binding site
1,416D, 1,417T, 1,418E, 1,419F, 1,421L, 1,477L, 1,481S, 1,521H, 1,522G, 1,524Q, 1,525N, 1,574H, 1,579D
CDD
cd06143 (PAN2_exo)
active site cd06143

1,416D

active site
1,416D, 1,417T, 1,418E, 1,419F, 1,421L, 1,477L, 1,481S, 1,521H, 1,522G, 1,524Q, 1,525N, 1,526D, 1,574H, 1,579D
CDD
cd06143 (PAN2_exo)

1,417T

active site
1,416D, 1,417T, 1,418E, 1,419F, 1,421L, 1,477L, 1,481S, 1,521H, 1,522G, 1,524Q, 1,525N, 1,526D, 1,574H, 1,579D
CDD
cd06143 (PAN2_exo)

1,418E

active site
1,416D, 1,417T, 1,418E, 1,419F, 1,421L, 1,477L, 1,481S, 1,521H, 1,522G, 1,524Q, 1,525N, 1,526D, 1,574H, 1,579D
CDD
cd06143 (PAN2_exo)

1,419F

active site
1,416D, 1,417T, 1,418E, 1,419F, 1,421L, 1,477L, 1,481S, 1,521H, 1,522G, 1,524Q, 1,525N, 1,526D, 1,574H, 1,579D
CDD
cd06143 (PAN2_exo)

1,421L

active site
1,416D, 1,417T, 1,418E, 1,419F, 1,421L, 1,477L, 1,481S, 1,521H, 1,522G, 1,524Q, 1,525N, 1,526D, 1,574H, 1,579D
CDD
cd06143 (PAN2_exo)

1,477L

active site
1,416D, 1,417T, 1,418E, 1,419F, 1,421L, 1,477L, 1,481S, 1,521H, 1,522G, 1,524Q, 1,525N, 1,526D, 1,574H, 1,579D
CDD
cd06143 (PAN2_exo)

1,481S

active site
1,416D, 1,417T, 1,418E, 1,419F, 1,421L, 1,477L, 1,481S, 1,521H, 1,522G, 1,524Q, 1,525N, 1,526D, 1,574H, 1,579D
CDD
cd06143 (PAN2_exo)

1,521H

active site
1,416D, 1,417T, 1,418E, 1,419F, 1,421L, 1,477L, 1,481S, 1,521H, 1,522G, 1,524Q, 1,525N, 1,526D, 1,574H, 1,579D
CDD
cd06143 (PAN2_exo)

1,522G

active site
1,416D, 1,417T, 1,418E, 1,419F, 1,421L, 1,477L, 1,481S, 1,521H, 1,522G, 1,524Q, 1,525N, 1,526D, 1,574H, 1,579D
CDD
cd06143 (PAN2_exo)

1,524Q

active site
1,416D, 1,417T, 1,418E, 1,419F, 1,421L, 1,477L, 1,481S, 1,521H, 1,522G, 1,524Q, 1,525N, 1,526D, 1,574H, 1,579D
CDD
cd06143 (PAN2_exo)

1,525N

active site
1,416D, 1,417T, 1,418E, 1,419F, 1,421L, 1,477L, 1,481S, 1,521H, 1,522G, 1,524Q, 1,525N, 1,526D, 1,574H, 1,579D
CDD
cd06143 (PAN2_exo)

1,526D

active site
1,416D, 1,417T, 1,418E, 1,419F, 1,421L, 1,477L, 1,481S, 1,521H, 1,522G, 1,524Q, 1,525N, 1,526D, 1,574H, 1,579D
CDD
cd06143 (PAN2_exo)

1,574H

active site
1,416D, 1,417T, 1,418E, 1,419F, 1,421L, 1,477L, 1,481S, 1,521H, 1,522G, 1,524Q, 1,525N, 1,526D, 1,574H, 1,579D
CDD
cd06143 (PAN2_exo)

1,579D

active site
1,416D, 1,417T, 1,418E, 1,419F, 1,421L, 1,477L, 1,481S, 1,521H, 1,522G, 1,524Q, 1,525N, 1,526D, 1,574H, 1,579D
CDD
cd06143 (PAN2_exo)

Protein sequence

>MCA_00852_1
MDNWHETLRVPIYRGPQIQPHYKLTSCLSFDNSQDILWSGSSDGYVTSFYSPSLRLYDSVHSQFNAPVSQIITNEKTVFA
LSPHSIKLLSRRGIIQDQIQNPTEFKSLSCMSLGREHATSPELLVGGNATNEILSLNLAAPGGSIGKEFTRIKLSDYGST
NIPHIGGDMSDPSAVKIVRTSHSLAVGKSNGVVDIIDTNSNQVVRSFKAHGGLISDMDAKETTLLTCGYSLRKNGLILDP
LVNIYDLRAGKSRTPIAFPAGAGFVRLHPKLSTCAFIASQTGQIQLIDFANSASSQVRLHQATLSTSGVLTSFEVSPSGD
FFALGEGSMIQLWNHSNIDSLADASFIERYSASVPLQFPVDPAAYILNTNTPATSLYPTPPMPIFDIDDYSAPLSSVGMP
YYQDELLSNWRPEPSMPMIFDEGMPPSRLPPEVLSQIQDATTFGVLNSSTNSSALNGAIPNSNMLKNTPDIRKTAPEFGG
IGVMGFFPPTPPSSNSSTEDPNNDSNQEEKVNKIRGNLAQKYVSILDLKKNTYRNAPKFISERQAISSRNRGGSNGASST
SSHPNLSSPNSAVEASGVYAKSSVETSFENNLDKATATKLETLMSSDLAEIQAGLIPQSYHRLEIKYSKFGIADFDFGYY
NETKFSGLEPQFVNSYCNPLLQLYRYSMPIFEFALNNLKHNTLDEKSLLSELGLLFDMLNKAQGQHVAATNFVKTLSAIP
QANALGLIVDDAHPINSFTNASLIHSSNASSNINLSSANSSNINNNISNNNSPAFQNIHQHHNHPNFANSSPAVDRRIEN
SNITNPGYFASGSAHNFDHTNMHHPNSAAGFVHQTVNNNNSRAGNAEEEDTIENVLVNLLGKLPSSNAIYRPKSEGMSSV
LTNGSHHAVSSNPSILYSQGLLLQAFSRFLMERMAIDERAYDNTSHKFDDIAGIHLTTDVSYPSCSNKSSRDQVIYTLQL
RAPVLDKQPIDPNIQKPNLFNTTKDPNSDTTSHDAFLQILQDSLEKKTTTRGFCNACHKYQSMVTTKTVYKLPEVLNLHV
PLDVPFATTNPAKQNIPKNNTNAHPPPSSKNSSISATHGSSNLYYSPSNSSSHDSNSSSHTPLFIPSANNGGSYQQQQPP
PGLHNFNNSQQGLMGYPSGGQHQQYEAPLFSPFISSKAPEFTNPLSGAAAHQYSQQQQQQPQLPQPYWRKERWPATEFAV
QRSNVTKSDSSNEERFKYTVIPSKDYNQSRGDQKYELIGLVVHISANPSGGSSSAGSDSILSSPSKGSNKSASSSSNGQS
LPGFECQQNNLIAFINVPIPPEDDPDTYKDNKDSSSVKEPKKQWHLFNDFLVKPVTESEVLNFSYLWKTPVVLLYQLVPP
TPRPPFDFEAWKAELDTSILYRDHFSTGFRESAKREYELLDPLTEAPQPGTLVAIDTEFVLLQHEETEIISDGTKSLLRP
KQMSLARVSVVRGQGPKEGVPFIDDFIETTDTIVDYLTEFSGIEPGDLDPWTSKRGGLVTLQTSYKRLWLLLNLGCVFIG
HGLQNDFRAINIQVPNNQVADTLNLFYLPEYKRRLSLKFLIYALLDEKVQTGNHDSIEDAVSALKLFKVYQKINRLGPDT
FTALLHKVYQEGKKYNFKPPPPSSNIPKPVELQKYIQSHIKE

GO term prediction

Biological Process

GO:0000289 nuclear-transcribed mRNA poly(A) tail shortening
GO:0006397 mRNA processing

Molecular Function

GO:0003676 nucleic acid binding
GO:0004535 poly(A)-specific ribonuclease activity
GO:0005515 protein binding

Cellular Component

GO:0031251 PAN complex

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Entry type

Status

Per-residue features