Protein
MIA_02796_1
Length
298 amino acids
Browser: contig03:1783573-1784470+
Protein function
| EGGNOG: | 0PGKF | DPH5 | S-adenosyl-L-methionine-dependent methyltransferase that catalyzes the trimethylation of the amino group of the modified target histidine residue in translation elongation factor 2 (EF- 2), to form an intermediate called diphthine. The three successive methylation reactions represent the second step of diphthamide biosynthesis (By similarity) |
|---|---|---|---|
| SGD closest match: | S000004162 | DPH5 | Diphthine methyl ester synthase |
Protein alignments
| %id | Aln length | E-value | ||
|---|---|---|---|---|
| MCA_05501_1 | 86.411% | 287 | 0.0 | MCA_05501_1 |
| A0A0J9XG75_GEOCN | 79.801% | 302 | 5.73e-172 | Similar to Saccharomyces cerevisiae YLR172C DPH5 Methyltransferase required for synthesis of diphthamide OS=Geotrichum candidum GN=BN980_GECA15s00615g PE=3 SV=1 |
| A0A167C7Z2_9ASCO | 79.038% | 291 | 7.37e-168 | Diphthine synthase OS=Sugiyamaella lignohabitans GN=DPH5 PE=3 SV=1 |
| A0A060T3E6_BLAAD | 78.547% | 289 | 6.91e-168 | ARAD1C32516p OS=Blastobotrys adeninivorans GN=GNLVRS02_ARAD1C32516g PE=3 SV=1 |
| DPH51_CANAL | 75.172% | 290 | 3.90e-166 | Diphthine methyl ester synthase 1 OS=Candida albicans (strain SC5314 / ATCC MYA-2876) GN=DPH5 PE=3 SV=2 |
| A0A1E3PEY5_9ASCO | 77.703% | 296 | 3.99e-164 | Diphthamide biosynthesis methyltransferase OS=Nadsonia fulvescens var. elongata DSM 6958 GN=NADFUDRAFT_27882 PE=3 SV=1 |
| DPH5_YARLI | 71.477% | 298 | 3.64e-161 | Diphthine methyl ester synthase OS=Yarrowia lipolytica (strain CLIB 122 / E 150) GN=DPH5 PE=3 SV=1 |
| UniRef50_Q6C1E0 | 71.477% | 298 | 8.42e-158 | Diphthine methyl ester synthase n=160 Tax=Eukaryota TaxID=2759 RepID=DPH5_YARLI |
| DPH5_YEAST | 69.580% | 286 | 1.99e-149 | Diphthine methyl ester synthase OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=DPH5 PE=1 SV=1 |
| A0A1E4T9H4_9ASCO | 67.958% | 284 | 1.47e-141 | Uncharacterized protein OS=Tortispora caseinolytica NRRL Y-17796 GN=CANCADRAFT_32998 PE=3 SV=1 |
Mitochondrial localization by mitoprotII
Probability of mitochondrial location: 0.1269
Protein family membership
- Diphthine synthase (IPR004551)
Domains and repeats
-
Domain
-
Domain
1
50
100
150
200
250
298
Detailed signature matches
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-
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cd11647 (Diphthine_...)
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MF_01084 (Diphthine...)
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PIRSF036432 (Diphth...)
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Residue annotation
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homodimer interfac...
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SAM binding site c...
Protein sequence
>MIA_02796_1 MLYLIGLGLSDVSDITVRGLETVRKCKRVYLEAYTSILMAADQASLEKYYGREVILADRELVEGGSDDILAGAQTEDVAF LVVGDPLGATTHTDLILRARELKIPYNIIHNASVMNAVGACGLQLYNFGQTVSLVFFTDSWKPESFYDKVLENRQLGLHT LLLLDIKVKEQSIENMARGRLIYEPPRYMSVATAAQQMLEIEEKRQQGAYGPNTPCIGVSRLGAPTQAFRADTLEQLADY DAGEPLHSLIILGNRVHDLETDFIVDFAIDEERLRKAIKDDAENFKKPEPPIVNMYDD
GO term prediction
Biological Process
GO:0008152 metabolic process
GO:0017183 peptidyl-diphthamide biosynthetic process from peptidyl-histidine
Molecular Function
GO:0004164 diphthine synthase activity
GO:0008168 methyltransferase activity
Cellular Component
None predicted.