MIA_02756_1

Protein

Length

394 amino acids

Protein function

EGGNOG:	0PFSQ	CBR1	Electron donor reductase for cytochrome b5. The cytochrome b5 NADH cytochrome b5 reductase electron transfer system supports the catalytic activity of several sterol biosynthetic enzymes (By similarity)
SGD closest match:	S000001305	CBR1	NADH-cytochrome b5 reductase 1
CGD closest match:	CAL0000182794	CBR1	NADH-cytochrome b5 reductase 1

Protein alignments

	%id	Aln length	E-value
A0A0J9XBF9_GEOCN	37.457%	291	3.18e-48	NADH-cytochrome b5 reductase OS=Geotrichum candidum GN=BN980_GECA08s02144g PE=3 SV=1
A0A1E3PM70_9ASCO	36.082%	291	9.63e-43	NADH-cytochrome b5 reductase OS=Nadsonia fulvescens var. elongata DSM 6958 GN=NADFUDRAFT_45895 PE=3 SV=1
A0A167FNN5_9ASCO	37.457%	291	2.34e-42	NADH-cytochrome b5 reductase OS=Sugiyamaella lignohabitans GN=CBR1 PE=3 SV=1
UniRef50_P0CP15	34.768%	302	1.14e-38	NADH-cytochrome b5 reductase 1 n=20 Tax=Tremellomycetes TaxID=155616 RepID=NCB5R_CRYNB
A0A060T2E0_BLAAD	35.739%	291	6.02e-42	NADH-cytochrome b5 reductase OS=Blastobotrys adeninivorans GN=GNLVRS02_ARAD1C27984g PE=3 SV=1
NCB5R_CANAL	36.426%	291	4.36e-40	NADH-cytochrome b5 reductase 1 OS=Candida albicans (strain SC5314 / ATCC MYA-2876) GN=CBR1 PE=3 SV=1
NCB5R_YARLI	32.646%	291	1.96e-39	NADH-cytochrome b5 reductase 1 OS=Yarrowia lipolytica (strain CLIB 122 / E 150) GN=CBR1 PE=3 SV=1
MCA_00705_1	32.423%	293	6.28e-38	MCA_00705_1
NCB5R_YEAST	33.333%	300	7.62e-37	NADH-cytochrome b5 reductase 1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=CBR1 PE=1 SV=2
A0A1E4TIF7_9ASCO	32.877%	292	3.07e-29	NADH-cytochrome b5 reductase OS=Tortispora caseinolytica NRRL Y-17796 GN=CANCADRAFT_71204 PE=3 SV=1

Mitochondrial localization by mitoprotII

Probability of mitochondrial location: 0.9905
Predicted cleavage: 55

Protein family membership

NADH:cytochrome b5 reductase (CBR) (IPR001834)

Domains and repeats

Domain
99 - 235
Riboflavin synthase-like beta-barrel (IPR017938)
Ferredoxin reductase-type FAD-binding domain (IPR017927)
Oxidoreductase, FAD-binding domain (IPR008333)
244 - 369
Oxidoreductase FAD/NAD(P)-binding (IPR001433)

1 50 100 150 200 250 300 350 394

Detailed signature matches

IPR001834

NADH:cytochrome b5 reductase (CBR)
1. PR00406 (CYTB5RDTASE)
  
  134 - 145
  
  PRINTS
  PR00406 (CYTB5RDTASE)
  
  355 - 363
  
  PRINTS
  PR00406 (CYTB5RDTASE)
  
  155 - 162
  
  PRINTS
  PR00406 (CYTB5RDTASE)
  
  243 - 262
  
  PRINTS
  PR00406 (CYTB5RDTASE)
IPR017938

Riboflavin synthase-like beta-barrel
1. SSF63380 (Riboflavi...)
  
  99 - 159
  
  SUPERFAMILY
  SSF63380 (Riboflavin synthase domain-like)
  
  186 - 226
  
  SUPERFAMILY
  SSF63380 (Riboflavin synthase domain-like)
IPR017927

Ferredoxin reductase-type FAD-binding domain
1. PS51384 (FAD_FR)
  
  103 - 235
  
  PROSITE profiles
  PS51384 (FAD_FR)
IPR008333

Oxidoreductase, FAD-binding domain
1. PF00970 (FAD_binding_6)
  
  108 - 164
  
  Pfam
  PF00970 (FAD_binding_6)
  
  171 - 227
  
  Pfam
  PF00970 (FAD_binding_6)
IPR001433

Oxidoreductase FAD/NAD(P)-binding
1. PF00175 (NAD_binding_1)
  
  244 - 369
  
  Pfam
  PF00175 (NAD_binding_1)

no IPR

Unintegrated signatures

G3DSA:2.40.30.10

93 - 222

GENE3D
G3DSA:2.40.30.10 (G3DSA:2.40.30.10)
G3DSA:3.40.50.80

223 - 382

GENE3D
G3DSA:3.40.50.80 (G3DSA:3.40.50.80)
NON_CYTOPLASM... (N...)

22 - 394

PHOBIUS
NON_CYTOPLASMIC_DOMAIN (Non cytoplasmic domain)
SIGNAL_PEPTIDE (Sig...)

1 - 21

PHOBIUS
SIGNAL_PEPTIDE (Signal Peptide)
SIGNAL_PEPTID... (S...)

17 - 21

PHOBIUS
SIGNAL_PEPTIDE_C_REGION (Signal peptide C-region)
SIGNAL_PEPTID... (S...)

5 - 16

PHOBIUS
SIGNAL_PEPTIDE_H_REGION (Signal peptide H-region)
SIGNAL_PEPTID... (S...)

1 - 4

PHOBIUS
SIGNAL_PEPTIDE_N_REGION (Signal peptide N-region)
SSF52343 (Ferredoxi...)

229 - 381

SUPERFAMILY
SSF52343 (Ferredoxin reductase-like, C-terminal NADP-linked domain)
SignalP-noTM

1 - 19

SignalP euk
SignalP-noTM (SignalP-noTM)
TMhelix

5 - 24

TMHMM
TMhelix (TMhelix)
cd06183 (cyt_b5_red...)

108 - 394

CDD
cd06183 (cyt_b5_reduct_like)
mobidb-lite (disord...)

40 - 88

MobiDB Lite
mobidb-lite (disorder_prediction)

Residue annotation

FAD binding pocket...

141C

FAD binding pocket
141C, 155R, 156N, 157Y, 158T, 198L, 199V, 200K, 202Y, 204S, 205G, 206N, 207V, 208S, 249T, 252I, 253P, 394F
CDD
cd06183 (cyt_b5_reduct_like)

155R

FAD binding pocket
141C, 155R, 156N, 157Y, 158T, 198L, 199V, 200K, 202Y, 204S, 205G, 206N, 207V, 208S, 249T, 252I, 253P, 394F
CDD
cd06183 (cyt_b5_reduct_like)

156N

FAD binding pocket
141C, 155R, 156N, 157Y, 158T, 198L, 199V, 200K, 202Y, 204S, 205G, 206N, 207V, 208S, 249T, 252I, 253P, 394F
CDD
cd06183 (cyt_b5_reduct_like)

157Y

FAD binding pocket
141C, 155R, 156N, 157Y, 158T, 198L, 199V, 200K, 202Y, 204S, 205G, 206N, 207V, 208S, 249T, 252I, 253P, 394F
CDD
cd06183 (cyt_b5_reduct_like)

158T

FAD binding pocket
141C, 155R, 156N, 157Y, 158T, 198L, 199V, 200K, 202Y, 204S, 205G, 206N, 207V, 208S, 249T, 252I, 253P, 394F
CDD
cd06183 (cyt_b5_reduct_like)

198L

FAD binding pocket
141C, 155R, 156N, 157Y, 158T, 198L, 199V, 200K, 202Y, 204S, 205G, 206N, 207V, 208S, 249T, 252I, 253P, 394F
CDD
cd06183 (cyt_b5_reduct_like)

199V

FAD binding pocket
141C, 155R, 156N, 157Y, 158T, 198L, 199V, 200K, 202Y, 204S, 205G, 206N, 207V, 208S, 249T, 252I, 253P, 394F
CDD
cd06183 (cyt_b5_reduct_like)

200K

FAD binding pocket
141C, 155R, 156N, 157Y, 158T, 198L, 199V, 200K, 202Y, 204S, 205G, 206N, 207V, 208S, 249T, 252I, 253P, 394F
CDD
cd06183 (cyt_b5_reduct_like)

202Y

FAD binding pocket
141C, 155R, 156N, 157Y, 158T, 198L, 199V, 200K, 202Y, 204S, 205G, 206N, 207V, 208S, 249T, 252I, 253P, 394F
CDD
cd06183 (cyt_b5_reduct_like)

204S

FAD binding pocket
141C, 155R, 156N, 157Y, 158T, 198L, 199V, 200K, 202Y, 204S, 205G, 206N, 207V, 208S, 249T, 252I, 253P, 394F
CDD
cd06183 (cyt_b5_reduct_like)

205G

FAD binding pocket
141C, 155R, 156N, 157Y, 158T, 198L, 199V, 200K, 202Y, 204S, 205G, 206N, 207V, 208S, 249T, 252I, 253P, 394F
CDD
cd06183 (cyt_b5_reduct_like)

206N

FAD binding pocket
141C, 155R, 156N, 157Y, 158T, 198L, 199V, 200K, 202Y, 204S, 205G, 206N, 207V, 208S, 249T, 252I, 253P, 394F
CDD
cd06183 (cyt_b5_reduct_like)

207V

FAD binding pocket
141C, 155R, 156N, 157Y, 158T, 198L, 199V, 200K, 202Y, 204S, 205G, 206N, 207V, 208S, 249T, 252I, 253P, 394F
CDD
cd06183 (cyt_b5_reduct_like)

208S

FAD binding pocket
141C, 155R, 156N, 157Y, 158T, 198L, 199V, 200K, 202Y, 204S, 205G, 206N, 207V, 208S, 249T, 252I, 253P, 394F
CDD
cd06183 (cyt_b5_reduct_like)

249T

FAD binding pocket
141C, 155R, 156N, 157Y, 158T, 198L, 199V, 200K, 202Y, 204S, 205G, 206N, 207V, 208S, 249T, 252I, 253P, 394F
CDD
cd06183 (cyt_b5_reduct_like)

252I

FAD binding pocket
141C, 155R, 156N, 157Y, 158T, 198L, 199V, 200K, 202Y, 204S, 205G, 206N, 207V, 208S, 249T, 252I, 253P, 394F
CDD
cd06183 (cyt_b5_reduct_like)

253P

FAD binding pocket
141C, 155R, 156N, 157Y, 158T, 198L, 199V, 200K, 202Y, 204S, 205G, 206N, 207V, 208S, 249T, 252I, 253P, 394F
CDD
cd06183 (cyt_b5_reduct_like)

394F

FAD binding pocket
141C, 155R, 156N, 157Y, 158T, 198L, 199V, 200K, 202Y, 204S, 205G, 206N, 207V, 208S, 249T, 252I, 253P, 394F
CDD
cd06183 (cyt_b5_reduct_like)
FAD binding motif ...

155R

FAD binding motif
155R, 157Y, 158T
CDD
cd06183 (cyt_b5_reduct_like)

157Y

FAD binding motif
155R, 157Y, 158T
CDD
cd06183 (cyt_b5_reduct_like)

158T

FAD binding motif
155R, 157Y, 158T
CDD
cd06183 (cyt_b5_reduct_like)
NAD binding pocket...

200K

NAD binding pocket
200K, 202Y, 249T, 250G, 294I, 295N, 296D, 320S, 332I, 360G, 361P, 362P, 363P, 364M
CDD
cd06183 (cyt_b5_reduct_like)

202Y

NAD binding pocket
200K, 202Y, 249T, 250G, 294I, 295N, 296D, 320S, 332I, 360G, 361P, 362P, 363P, 364M
CDD
cd06183 (cyt_b5_reduct_like)

249T

NAD binding pocket
200K, 202Y, 249T, 250G, 294I, 295N, 296D, 320S, 332I, 360G, 361P, 362P, 363P, 364M
CDD
cd06183 (cyt_b5_reduct_like)

250G

NAD binding pocket
200K, 202Y, 249T, 250G, 294I, 295N, 296D, 320S, 332I, 360G, 361P, 362P, 363P, 364M
CDD
cd06183 (cyt_b5_reduct_like)

294I

NAD binding pocket
200K, 202Y, 249T, 250G, 294I, 295N, 296D, 320S, 332I, 360G, 361P, 362P, 363P, 364M
CDD
cd06183 (cyt_b5_reduct_like)

295N

NAD binding pocket
200K, 202Y, 249T, 250G, 294I, 295N, 296D, 320S, 332I, 360G, 361P, 362P, 363P, 364M
CDD
cd06183 (cyt_b5_reduct_like)

296D

NAD binding pocket
200K, 202Y, 249T, 250G, 294I, 295N, 296D, 320S, 332I, 360G, 361P, 362P, 363P, 364M
CDD
cd06183 (cyt_b5_reduct_like)

320S

NAD binding pocket
200K, 202Y, 249T, 250G, 294I, 295N, 296D, 320S, 332I, 360G, 361P, 362P, 363P, 364M
CDD
cd06183 (cyt_b5_reduct_like)

332I

NAD binding pocket
200K, 202Y, 249T, 250G, 294I, 295N, 296D, 320S, 332I, 360G, 361P, 362P, 363P, 364M
CDD
cd06183 (cyt_b5_reduct_like)

360G

NAD binding pocket
200K, 202Y, 249T, 250G, 294I, 295N, 296D, 320S, 332I, 360G, 361P, 362P, 363P, 364M
CDD
cd06183 (cyt_b5_reduct_like)

361P

NAD binding pocket
200K, 202Y, 249T, 250G, 294I, 295N, 296D, 320S, 332I, 360G, 361P, 362P, 363P, 364M
CDD
cd06183 (cyt_b5_reduct_like)

362P

NAD binding pocket
200K, 202Y, 249T, 250G, 294I, 295N, 296D, 320S, 332I, 360G, 361P, 362P, 363P, 364M
CDD
cd06183 (cyt_b5_reduct_like)

363P

NAD binding pocket
200K, 202Y, 249T, 250G, 294I, 295N, 296D, 320S, 332I, 360G, 361P, 362P, 363P, 364M
CDD
cd06183 (cyt_b5_reduct_like)

364M

NAD binding pocket
200K, 202Y, 249T, 250G, 294I, 295N, 296D, 320S, 332I, 360G, 361P, 362P, 363P, 364M
CDD
cd06183 (cyt_b5_reduct_like)
phosphate binding ...

205G

phosphate binding motif
205G, 208S, 211I, 217G, 225P, 230G
CDD
cd06183 (cyt_b5_reduct_like)

208S

phosphate binding motif
205G, 208S, 211I, 217G, 225P, 230G
CDD
cd06183 (cyt_b5_reduct_like)

211I

phosphate binding motif
205G, 208S, 211I, 217G, 225P, 230G
CDD
cd06183 (cyt_b5_reduct_like)

217G

phosphate binding motif
205G, 208S, 211I, 217G, 225P, 230G
CDD
cd06183 (cyt_b5_reduct_like)

225P

phosphate binding motif
205G, 208S, 211I, 217G, 225P, 230G
CDD
cd06183 (cyt_b5_reduct_like)

230G

phosphate binding motif
205G, 208S, 211I, 217G, 225P, 230G
CDD
cd06183 (cyt_b5_reduct_like)
beta-alpha-beta st...

244M

beta-alpha-beta structure motif
244M, 248G, 249T, 250G, 251I, 253P
CDD
cd06183 (cyt_b5_reduct_like)

248G

beta-alpha-beta structure motif
244M, 248G, 249T, 250G, 251I, 253P
CDD
cd06183 (cyt_b5_reduct_like)

249T

beta-alpha-beta structure motif
244M, 248G, 249T, 250G, 251I, 253P
CDD
cd06183 (cyt_b5_reduct_like)

250G

beta-alpha-beta structure motif
244M, 248G, 249T, 250G, 251I, 253P
CDD
cd06183 (cyt_b5_reduct_like)

251I

beta-alpha-beta structure motif
244M, 248G, 249T, 250G, 251I, 253P
CDD
cd06183 (cyt_b5_reduct_like)

253P

beta-alpha-beta structure motif
244M, 248G, 249T, 250G, 251I, 253P
CDD
cd06183 (cyt_b5_reduct_like)

Protein sequence

>MIA_02756_1
MLYQLASFLLFTIAVLATAHRLALLPPRVLRAIAARSSRNKSLRNISSNPPPRSTPKSPSSSSQSHPQTQIDPEIGPPAD
PNIPNGASSGLSLLNASSVLLRKTPASFPLIHKSHLSESTAIYRFALPRPTDSLGLAPGQCITVLAPLHGKILRRNYTPV
TLDSDFYANTKSTASSKTTTQHNTTTNYTNTRGYFELLVKSYPSGNVSKHIASLPLGACIQVLGPSSSAGSFVYSPHMAR
HINMIAAGTGIIPIYQLLLYILSSPDLSSSHHAHHYPHHLNPNHAHPSISLIYINDSERDVLLKRQLDSLPIDITYIYTS
SLPDSQSSDSPIILSRDIFTKHTLLPDKNNNQSVRLLLSGPPPMVSAMRKVAVDAGYAPARKGPSRPDDQVFVF

GO term prediction

Biological Process

GO:0055114 oxidation-reduction process

Molecular Function

GO:0016491 oxidoreductase activity

Cellular Component

None predicted.

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Entry type

Status

Per-residue features