MIA_02719_1

Protein

Length

2,125 amino acids

Protein function

EGGNOG:	0PGAG	BRR2	helicase
SGD closest match:	S000000974	BRR2	Pre-mRNA-splicing helicase BRR2
CGD closest match:	CAL0000181172	orf19.3098	ATP-dependent RNA helicase

Protein alignments

	%id	Aln length
MCA_01507_1	68.568%	2192	MCA_01507_1
A0A0J9XKE9_GEOCN	63.924%	2151	Similar to Saccharomyces cerevisiae YER172C BRR2 RNA-dependent ATPase RNA helicase (DEIH box) OS=Geotrichum candidum GN=BN980_GECA23s00197g PE=4 SV=1
A0A060TE03_BLAAD	56.152%	2162	ARAD1D03982p OS=Blastobotrys adeninivorans GN=GNLVRS02_ARAD1D03982g PE=4 SV=1
UniRef50_A0A060TE03	56.152%	2162	ARAD1D03982p n=2 Tax=Saccharomycetales TaxID=4892 RepID=A0A060TE03_BLAAD
A0A1E3PM47_9ASCO	56.536%	2142	Sec63-domain-containing protein OS=Nadsonia fulvescens var. elongata DSM 6958 GN=NADFUDRAFT_50185 PE=4 SV=1
A0A167FZE2_9ASCO	58.333%	1968	Brr2p OS=Sugiyamaella lignohabitans GN=BRR2 PE=4 SV=1
Q6C0Y6_YARLI	49.015%	2132	YALI0F20724p OS=Yarrowia lipolytica (strain CLIB 122 / E 150) GN=YALI0_F20724g PE=4 SV=1
A0A1E4TCW3_9ASCO	42.646%	2162	Uncharacterized protein OS=Tortispora caseinolytica NRRL Y-17796 GN=CANCADRAFT_4197 PE=4 SV=1
BRR2_YEAST	42.476%	2173	Pre-mRNA-splicing helicase BRR2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=BRR2 PE=1 SV=2
A0A1D8PMR5_CANAL	42.697%	2054	ATP-dependent RNA helicase OS=Candida albicans (strain SC5314 / ATCC MYA-2876) GN=orf19.3098 PE=4 SV=1

Mitochondrial localization by mitoprotII

Probability of mitochondrial location: 0.0207

Protein family membership

None predicted.

Domains and repeats

Domain
457 - 671
Helicase superfamily 1/2, ATP-binding domain (IPR014001)
DEAD/DEAH box helicase domain (IPR011545)
692 - 898
Helicase, C-terminal (IPR001650)
961 - 1,270
Sec63 domain (IPR004179)
1,305 - 1,510
Helicase superfamily 1/2, ATP-binding domain (IPR014001)
DEAD/DEAH box helicase domain (IPR011545)
1,791 - 2,109
Sec63 domain (IPR004179)
Domain
468 - 639
P-loop containing nucleoside triphosphate hydrolase (IPR027417)
668 - 721
P-loop containing nucleoside triphosphate hydrolase (IPR027417)
759 - 852
P-loop containing nucleoside triphosphate hydrolase (IPR027417)
1,211 - 1,487
P-loop containing nucleoside triphosphate hydrolase (IPR027417)
1,496 - 1,700
P-loop containing nucleoside triphosphate hydrolase (IPR027417)
2,006 - 2,113
Immunoglobulin E-set (IPR014756)
Domain
869 - 973
Winged helix-turn-helix DNA-binding domain (IPR011991)
1,162 - 1,273
C2 domain (IPR000008)
1,995 - 2,122
C2 domain (IPR000008)

1 200 400 600 800 1000 1200 1400 1600 1800 2000 2125

Detailed signature matches

IPR014001

Helicase superfamily 1/2, ATP-binding domain
1. PS51192 (HELICASE_A...)
  
  1,318 - 1,494
  
  PROSITE profiles
  PS51192 (HELICASE_ATP_BIND_1)
  
  470 - 654
  
  PROSITE profiles
  PS51192 (HELICASE_ATP_BIND_1)
2. SM00487 (ultradead3)
  
  457 - 671
  
  SMART
  SM00487 (ultradead3)
  
  1,305 - 1,510
  
  SMART
  SM00487 (ultradead3)
IPR011545

DEAD/DEAH box helicase domain
1. PF00270 (DEAD)
  
  463 - 640
  
  Pfam
  PF00270 (DEAD)
  
  1,311 - 1,475
  
  Pfam
  PF00270 (DEAD)
IPR027417

P-loop containing nucleoside triphosphate hydrolase
1. SSF52540 (P-loop co...)
  
  759 - 852
  
  SUPERFAMILY
  SSF52540 (P-loop containing nucleoside triphosphate hydrolases)
  
  668 - 721
  
  SUPERFAMILY
  SSF52540 (P-loop containing nucleoside triphosphate hydrolases)
  
  468 - 639
  
  SUPERFAMILY
  SSF52540 (P-loop containing nucleoside triphosphate hydrolases)
  
  1,496 - 1,700
  
  SUPERFAMILY
  SSF52540 (P-loop containing nucleoside triphosphate hydrolases)
  
  1,211 - 1,487
  
  SUPERFAMILY
  SSF52540 (P-loop containing nucleoside triphosphate hydrolases)
IPR001650

Helicase, C-terminal
1. PS51194 (HELICASE_CTER)
  
  692 - 898
  
  PROSITE profiles
  PS51194 (HELICASE_CTER)
2. SM00490 (helicmild6)
  
  753 - 841
  
  SMART
  SM00490 (helicmild6)
3. PF00271 (Helicase_C)
  
  695 - 841
  
  Pfam
  PF00271 (Helicase_C)
IPR011991

Winged helix-turn-helix DNA-binding domain
1. SSF46785 ("Winged h...)
  
  869 - 973
  
  SUPERFAMILY
  SSF46785 ("Winged helix" DNA-binding domain)
IPR004179

Sec63 domain
1. SM00973 (Sec63_2)
  
  1,791 - 2,109
  
  SMART
  SM00973 (Sec63_2)
  
  961 - 1,270
  
  SMART
  SM00973 (Sec63_2)
2. PF02889 (Sec63)
  
  1,791 - 2,107
  
  Pfam
  PF02889 (Sec63)
  
  961 - 1,266
  
  Pfam
  PF02889 (Sec63)
IPR000008

C2 domain
1. G3DSA:2.60.40...
  
  1,162 - 1,273
  
  GENE3D
  G3DSA:2.60.40.150 (G3DSA:2.60.40.150)
  
  1,995 - 2,122
  
  GENE3D
  G3DSA:2.60.40.150 (G3DSA:2.60.40.150)
IPR014756

Immunoglobulin E-set
1. SSF81296 (E set dom...)
  
  2,006 - 2,113
  
  SUPERFAMILY
  SSF81296 (E set domains)

no IPR

Unintegrated signatures

G3DSA:1.10.15...

1,935 - 1,994

GENE3D
G3DSA:1.10.150.20 (G3DSA:1.10.150.20)
G3DSA:1.10.33...

990 - 1,105

GENE3D
G3DSA:1.10.3380.10 (G3DSA:1.10.3380.10)

1,819 - 1,934

GENE3D
G3DSA:1.10.3380.10 (G3DSA:1.10.3380.10)
G3DSA:3.40.50...

434 - 661

GENE3D
G3DSA:3.40.50.300 (G3DSA:3.40.50.300)

662 - 876

GENE3D
G3DSA:3.40.50.300 (G3DSA:3.40.50.300)

1,526 - 1,713

GENE3D
G3DSA:3.40.50.300 (G3DSA:3.40.50.300)

1,289 - 1,501

GENE3D
G3DSA:3.40.50.300 (G3DSA:3.40.50.300)
PIRSF039073 (BRR2)

1 - 2,121

PIRSF
PIRSF039073 (BRR2)
SSF158702 (Sec63 N-...)

990 - 1,163

SUPERFAMILY
SSF158702 (Sec63 N-terminal domain-like)

1,820 - 1,995

SUPERFAMILY
SSF158702 (Sec63 N-terminal domain-like)
cd00046 (DEXDc)

1,334 - 1,473

CDD
cd00046 (DEXDc)

477 - 636

CDD
cd00046 (DEXDc)
cd00079 (HELICc)

665 - 841

CDD
cd00079 (HELICc)
mobidb-lite (disord...)

1 - 26

MobiDB Lite
mobidb-lite (disorder_prediction)

195 - 234

MobiDB Lite
mobidb-lite (disorder_prediction)

Residue annotation

ATP binding site c...

486G

ATP binding site
486G, 487A, 488G, 489K, 490T
CDD
cd00046 (DEXDc)

487A

ATP binding site
486G, 487A, 488G, 489K, 490T
CDD
cd00046 (DEXDc)

488G

ATP binding site
486G, 487A, 488G, 489K, 490T
CDD
cd00046 (DEXDc)

489K

ATP binding site
486G, 487A, 488G, 489K, 490T
CDD
cd00046 (DEXDc)

490T

ATP binding site
486G, 487A, 488G, 489K, 490T
CDD
cd00046 (DEXDc)
putative Mg++ bind...

596D

putative Mg++ binding site
596D, 597E, 598I, 599H
CDD
cd00046 (DEXDc)

597E

putative Mg++ binding site
596D, 597E, 598I, 599H
CDD
cd00046 (DEXDc)

598I

putative Mg++ binding site
596D, 597E, 598I, 599H
CDD
cd00046 (DEXDc)

599H

putative Mg++ binding site
596D, 597E, 598I, 599H
CDD
cd00046 (DEXDc)
nucleotide binding...

707H

nucleotide binding region
707H, 708S, 709R, 710K, 766H, 767H, 793T, 794A, 795T
CDD
cd00079 (HELICc)

708S

nucleotide binding region
707H, 708S, 709R, 710K, 766H, 767H, 793T, 794A, 795T
CDD
cd00079 (HELICc)

709R

nucleotide binding region
707H, 708S, 709R, 710K, 766H, 767H, 793T, 794A, 795T
CDD
cd00079 (HELICc)

710K

nucleotide binding region
707H, 708S, 709R, 710K, 766H, 767H, 793T, 794A, 795T
CDD
cd00079 (HELICc)

766H

nucleotide binding region
707H, 708S, 709R, 710K, 766H, 767H, 793T, 794A, 795T
CDD
cd00079 (HELICc)

767H

nucleotide binding region
707H, 708S, 709R, 710K, 766H, 767H, 793T, 794A, 795T
CDD
cd00079 (HELICc)

793T

nucleotide binding region
707H, 708S, 709R, 710K, 766H, 767H, 793T, 794A, 795T
CDD
cd00079 (HELICc)

794A

nucleotide binding region
707H, 708S, 709R, 710K, 766H, 767H, 793T, 794A, 795T
CDD
cd00079 (HELICc)

795T

nucleotide binding region
707H, 708S, 709R, 710K, 766H, 767H, 793T, 794A, 795T
CDD
cd00079 (HELICc)
ATP-binding site c...

801N

ATP-binding site
801N, 832Q, 836R, 839R
CDD
cd00079 (HELICc)

832Q

ATP-binding site
801N, 832Q, 836R, 839R
CDD
cd00079 (HELICc)

836R

ATP-binding site
801N, 832Q, 836R, 839R
CDD
cd00079 (HELICc)

839R

ATP-binding site
801N, 832Q, 836R, 839R
CDD
cd00079 (HELICc)
ATP binding site c...

1,334G

ATP binding site
1,334G, 1,335N, 1,336G, 1,337K, 1,338I
CDD
cd00046 (DEXDc)

1,335N

ATP binding site
1,334G, 1,335N, 1,336G, 1,337K, 1,338I
CDD
cd00046 (DEXDc)

1,336G

ATP binding site
1,334G, 1,335N, 1,336G, 1,337K, 1,338I
CDD
cd00046 (DEXDc)

1,337K

ATP binding site
1,334G, 1,335N, 1,336G, 1,337K, 1,338I
CDD
cd00046 (DEXDc)

1,338I

ATP binding site
1,334G, 1,335N, 1,336G, 1,337K, 1,338I
CDD
cd00046 (DEXDc)
putative Mg++ bind...

1,436D

putative Mg++ binding site
1,436D, 1,437D, 1,438A, 1,439H
CDD
cd00046 (DEXDc)

1,437D

putative Mg++ binding site
1,436D, 1,437D, 1,438A, 1,439H
CDD
cd00046 (DEXDc)

1,438A

putative Mg++ binding site
1,436D, 1,437D, 1,438A, 1,439H
CDD
cd00046 (DEXDc)

1,439H

putative Mg++ binding site
1,436D, 1,437D, 1,438A, 1,439H
CDD
cd00046 (DEXDc)

Protein sequence

>MIA_02719_1
MSNKVLTADRRFVTRNNNESTAEPESLAGKISVKDLGSRATRAKVEKDTIDKKIKTEVSEPKKRNKKGILHSSDTGTTLV
EMSGYAEGLNYYPRTPETREVFDLIMSWIQAFLEDVPHETIRSAADAALTVLKDENMSDKEKKTEIEDILATKISGDKFS
ELLKLSKRITDYINPEEDAELQESRVGEDAGVAVTFEGSDNDDDDIPIDSDDEESEEETPQEYSQENANDMNEDTEVIGK
RRKAQDKDDTIPAHEIDEYWLQRQVGSIYSDAQVTHQKTTEIFKILSSPAPLNEVENEIMEIFDFEHFEMTKKMCKNRDR
IVWLTRLAQASTDSEKDEVTKEMVRQGLDSLLREIHGSTINDTHDKDVDMEDVPVDNQYLNKSIEREPKVVDLDNLVFEQ
GHRINNVSELRLPEGTTKSVTKTYEQYDIPPPRPAQNHVTLVPISSLPLWAQPAFKTTKTLNPVQSSVFPSAFNSSENIL
LCAPTGAGKTNVALLTILETISHYLDLETNNIDLDSFKIVYISPLKALVQEQVREFTKKLESYGIKVAELTGDRNLTKQQ
IAETQMIVTTPEKWDVITRKSSDTSYTNLVRLIIIDEIHLLHDERGPVLESIVSRTLRREAATGEPVRLVGLSATLPNYK
DVARFLRVDVNKGMFFFDSSYRPTPLAQSYIGVTEKKAFKRYQAMNDACYDKVLQYAGQHQVIIFVHSRKETAKTAKYLR
DKAAEEGTLRRFLKSDSAATKLLTSESESVQSADLKDILPSGFAIHHAGLSRADRTSAEDLFRDGYVQVLVSTATLAWGV
NLPAHTVIIKGTQVYSPEKGKWTELSPQDVLQMLGRAGRPGYDKSGDGVIITAHTELSYYMSLINASLPIESQMMAKLVD
CINAEVALGTITSRKDAIEWLGYTYLYVRMLKSPKDYRVGEEYANDPTLINKRQDLSHSALLVLHENNLVRYDPNTGAVK
PTELGRIASHFYISHTSMATYNSNLKPFLTNIEIFRIFSMSEEFKYIPVRQEEKLELSKLLEKAPIPIKETVEEPSAKIN
ILLQAYISRLKLDGFALMADMVYITQSAGRLIRAMYEIALRKGWAPLTRITLDLCKMVEKRLWLSNSPFRQFPNVPGDVI
KRTEASQLPWSKYFDLDSPAEVGQVIRLDSKGSLVYQMLQQFPRLHVDARFRPITLSLLRIELAITPEFTEWNREIHGTA
LTYLLMVEDDDGESILFSDYFILRERYFNSEEPHYVDFTVPISEPIPANYFVSLISEKWLHCESRIVLPFNNLTLPEKFP
AHTPIYDMEPVSVSSLGRKFKSLFDFDFFNKIQTQSFHTLFNTDDSVFIGASGGNGKIVCGELALLRHISEENEGNVVYL
APFQEQVNDRYNDWVKRFGGIIDVSEIQKLTGELSADLKLVENASVILATPVQWDLISRRWKKRANVRKVSLVIADDAHL
IGGMNGAIYENVLSRFRFIATQEEQPIRIVALSVSLANGKDFGKWIGATSHSIFNFPPGERQIPLELHLQSFSIPHHPSF
MISLAHPTYAAIRNLKERESTLIFVENRQQCILTASDLVRLSHADGNVDCFLLAESIKPFLDKIQDENLKESLASGIGYY
YPYMEKSDKKIVEHLFKKKAIQVLLATRESCWSCPNATLVIIMGTQLYEGREHRYIDYTISEILQMIGNASDSDDFNQAL
ILTNTSKREYYRKFLSEALPIESHMPSYLADAFITEISEGIISARNDCMGWLMYTYFFSRLTANPTFYDLTDTSYDGINA
YLSELVENTLDSLAEAKMIELDAESDDSISPLNGAIISSYYNVSYYTMQTFILSLNEKTRLKGMLEIVTSATEFESLPVR
THEDELLVKIHSHLPMKMARFSPYSAHFKAYVLLEAHISRITLSPDLTADQSIILSRVISLLAACVDVLSGEGNLNATYA
MDLSQMIVQAIWNKDSPLKQIPYFTSDMIQRSKDENIKSVYDFVMIDDDVRERILQINEEDPRMVAIANFVNKYPNIEIS
YELVDETIDTDEQSTILVRVEREVDEEDPVVDVTVESQFYTQPKIENWWLVIGETSTNQIYGIRRVMLSKPQQEFKLKFS
IPTGGEHKLSVFCMSDSYFDVDKEIELSVQVKEAESDQEMEDDDE

GO term prediction

Biological Process

None predicted.

Molecular Function

GO:0003676 nucleic acid binding
GO:0005524 ATP binding

Cellular Component

None predicted.

Filter view on

Entry type

Status

Per-residue features

MIA_02719_1

Protein function

Protein alignments

Mitochondrial localization by mitoprotII

Protein family membership

Domains and repeats

Detailed signature matches

Residue annotation

Protein sequence

GO term prediction

Biological Process

Molecular Function

Cellular Component