MIA_02580_1

Protein

Length

1,848 amino acids

Protein function

EGGNOG:	0PFY5	FAS2	fatty acid synthase
SGD closest match:	S000006152	FAS2	Fatty acid synthase subunit alpha
CGD closest match:	CAL0000182497	FAS2	Trifunctional fatty acid synthase subunit

Protein alignments

	%id	Aln length
MCA_05244_1	86.105%	1864	MCA_05244_1
A0A0J9XEN9_GEOCN	80.829%	1857	Similar to Saccharomyces cerevisiae YPL231W FAS2 Alpha subunit of fatty acid synthetase OS=Geotrichum candidum GN=BN980_GECA11s00153g PE=3 SV=1
A0A060T3L0_BLAAD	75.148%	1859	ARAD1A16676p OS=Blastobotrys adeninivorans GN=GNLVRS02_ARAD1A16676g PE=3 SV=1
A0A1E3PQN4_9ASCO	72.830%	1855	Alpha subunit of fatty acid synthase OS=Nadsonia fulvescens var. elongata DSM 6958 GN=FAS2 PE=3 SV=1
A0A161HHZ5_9ASCO	75.194%	1802	Trifunctional fatty acid synthase subunit FAS2 OS=Sugiyamaella lignohabitans GN=FAS2 PE=3 SV=1
A0A1D8PK65_CANAL	66.490%	1892	Trifunctional fatty acid synthase subunit OS=Candida albicans (strain SC5314 / ATCC MYA-2876) GN=FAS2 PE=3 SV=1
UniRef50_P43098	66.244%	1893	Fatty acid synthase subunit alpha n=21 Tax=saccharomyceta TaxID=716545 RepID=FAS2_CANAX
Q6CE16_YARLI	66.702%	1862	YALI0B19382p OS=Yarrowia lipolytica (strain CLIB 122 / E 150) GN=YALI0_B19382g PE=4 SV=1
FAS2_YEAST	62.771%	1891	Fatty acid synthase subunit alpha OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=FAS2 PE=1 SV=2
A0A1E4TI24_9ASCO	61.946%	1850	Uncharacterized protein OS=Tortispora caseinolytica NRRL Y-17796 GN=CANCADRAFT_32052 PE=4 SV=1

Mitochondrial localization by mitoprotII

Probability of mitochondrial location: 0.0907

Protein family membership

Holo-[acyl carrier protein] synthase (IPR002582)
- Fatty acid synthase alpha subunit, yeast (IPR026025)

Domains and repeats

Domain
12 - 63
Acyl transferase/acyl hydrolase/lysophospholipase (IPR016035)
636 - 877
NAD(P)-binding domain (IPR016040)
1,145 - 1,348
Beta-ketoacyl synthase, N-terminal (IPR014030)
1,487 - 1,576
Beta-ketoacyl synthase, C-terminal (IPR014031)
1,728 - 1,848
4'-phosphopantetheinyl transferase superfamily (IPR008278)
Phosphopantetheine-protein transferase domain (IPR004568)
Domain
981 - 1,044
Thiolase-like (IPR016039)
1,081 - 1,391
Thiolase-like (IPR016039)
1,483 - 1,624
Thiolase-like (IPR016039)

1 200 400 600 800 1000 1200 1400 1600 1848

Detailed signature matches

IPR002582

Holo-[acyl carrier protein] synthase
1. MF_00101 (AcpS)
  
  1,731 - 1,848
  
  HAMAP
  MF_00101 (AcpS)
IPR026025

Fatty acid synthase alpha subunit, yeast
1. PIRSF000454 (FAS_ye...)
  
  1 - 1,848
  
  PIRSF
  PIRSF000454 (FAS_yeast_alpha)
IPR016035

Acyl transferase/acyl hydrolase/lysophospholipase
1. SSF52151 (FabD/lyso...)
  
  12 - 63
  
  SUPERFAMILY
  SSF52151 (FabD/lysophospholipase-like)
IPR016040

NAD(P)-binding domain
1. SSF51735 (NAD(P)-bi...)
  
  636 - 877
  
  SUPERFAMILY
  SSF51735 (NAD(P)-binding Rossmann-fold domains)
IPR016039

Thiolase-like
1. SSF53901 (Thiolase-...)
  
  1,150 - 1,362
  
  SUPERFAMILY
  SSF53901 (Thiolase-like)
  
  981 - 1,044
  
  SUPERFAMILY
  SSF53901 (Thiolase-like)
  
  1,487 - 1,620
  
  SUPERFAMILY
  SSF53901 (Thiolase-like)
  
  1,324 - 1,391
  
  SUPERFAMILY
  SSF53901 (Thiolase-like)
2. G3DSA:3.40.47.10
  
  1,081 - 1,367
  
  GENE3D
  G3DSA:3.40.47.10 (G3DSA:3.40.47.10)
  
  1,483 - 1,624
  
  GENE3D
  G3DSA:3.40.47.10 (G3DSA:3.40.47.10)
IPR014030

Beta-ketoacyl synthase, N-terminal
1. PF00109 (ketoacyl-synt)
  
  1,145 - 1,348
  
  Pfam
  PF00109 (ketoacyl-synt)
IPR014031

Beta-ketoacyl synthase, C-terminal
1. PF02801 (Ketoacyl-s...)
  
  1,487 - 1,576
  
  Pfam
  PF02801 (Ketoacyl-synt_C)
IPR008278

4'-phosphopantetheinyl transferase superfamily
1. PF01648 (ACPS)
  
  1,731 - 1,839
  
  Pfam
  PF01648 (ACPS)
2. SSF56214 (4'-phosph...)
  
  1,730 - 1,847
  
  SUPERFAMILY
  SSF56214 (4'-phosphopantetheinyl transferase)
3. G3DSA:3.90.47...
  
  1,728 - 1,848
  
  GENE3D
  G3DSA:3.90.470.20 (G3DSA:3.90.470.20)
IPR004568

Phosphopantetheine-protein transferase domain
1. TIGR00556
  
  1,729 - 1,846
  
  TIGRFAMs
  TIGR00556 (TIGR00556)
2. PD004282
  
  1,734 - 1,821
  
  ProDom
  PD004282 (PD004282)

no IPR

Unintegrated signatures

Coil

1,843 - 1,848

COILS
Coil (Coil)
G3DSA:3.40.36...

4 - 73

GENE3D
G3DSA:3.40.366.10 (G3DSA:3.40.366.10)
G3DSA:3.40.50...

636 - 881

GENE3D
G3DSA:3.40.50.720 (G3DSA:3.40.50.720)
cd00828 (elong_cond...)

1,097 - 1,618

CDD
cd00828 (elong_cond_enzymes)
cd08950 (KR_fFAS_SD...)

632 - 893

CDD
cd08950 (KR_fFAS_SDR_c_like)
mobidb-lite (disord...)

1,307 - 1,337

MobiDB Lite
mobidb-lite (disorder_prediction)

484 - 504

MobiDB Lite
mobidb-lite (disorder_prediction)

Residue annotation

putative NAD(P) bi...

645G

putative NAD(P) binding site
645G, 647G, 648A, 651I, 670T, 671S, 672R, 734F, 735A, 736A, 788P, 789L, 790S, 802Y, 806K, 832I, 833G, 834W, 835T
CDD
cd08950 (KR_fFAS_SDR_c_like)

647G

putative NAD(P) binding site
645G, 647G, 648A, 651I, 670T, 671S, 672R, 734F, 735A, 736A, 788P, 789L, 790S, 802Y, 806K, 832I, 833G, 834W, 835T
CDD
cd08950 (KR_fFAS_SDR_c_like)

648A

putative NAD(P) binding site
645G, 647G, 648A, 651I, 670T, 671S, 672R, 734F, 735A, 736A, 788P, 789L, 790S, 802Y, 806K, 832I, 833G, 834W, 835T
CDD
cd08950 (KR_fFAS_SDR_c_like)

651I

putative NAD(P) binding site
645G, 647G, 648A, 651I, 670T, 671S, 672R, 734F, 735A, 736A, 788P, 789L, 790S, 802Y, 806K, 832I, 833G, 834W, 835T
CDD
cd08950 (KR_fFAS_SDR_c_like)

670T

putative NAD(P) binding site
645G, 647G, 648A, 651I, 670T, 671S, 672R, 734F, 735A, 736A, 788P, 789L, 790S, 802Y, 806K, 832I, 833G, 834W, 835T
CDD
cd08950 (KR_fFAS_SDR_c_like)

671S

putative NAD(P) binding site
645G, 647G, 648A, 651I, 670T, 671S, 672R, 734F, 735A, 736A, 788P, 789L, 790S, 802Y, 806K, 832I, 833G, 834W, 835T
CDD
cd08950 (KR_fFAS_SDR_c_like)

672R

putative NAD(P) binding site
645G, 647G, 648A, 651I, 670T, 671S, 672R, 734F, 735A, 736A, 788P, 789L, 790S, 802Y, 806K, 832I, 833G, 834W, 835T
CDD
cd08950 (KR_fFAS_SDR_c_like)

734F

putative NAD(P) binding site
645G, 647G, 648A, 651I, 670T, 671S, 672R, 734F, 735A, 736A, 788P, 789L, 790S, 802Y, 806K, 832I, 833G, 834W, 835T
CDD
cd08950 (KR_fFAS_SDR_c_like)

735A

putative NAD(P) binding site
645G, 647G, 648A, 651I, 670T, 671S, 672R, 734F, 735A, 736A, 788P, 789L, 790S, 802Y, 806K, 832I, 833G, 834W, 835T
CDD
cd08950 (KR_fFAS_SDR_c_like)

736A

putative NAD(P) binding site
645G, 647G, 648A, 651I, 670T, 671S, 672R, 734F, 735A, 736A, 788P, 789L, 790S, 802Y, 806K, 832I, 833G, 834W, 835T
CDD
cd08950 (KR_fFAS_SDR_c_like)

788P

putative NAD(P) binding site
645G, 647G, 648A, 651I, 670T, 671S, 672R, 734F, 735A, 736A, 788P, 789L, 790S, 802Y, 806K, 832I, 833G, 834W, 835T
CDD
cd08950 (KR_fFAS_SDR_c_like)

789L

putative NAD(P) binding site
645G, 647G, 648A, 651I, 670T, 671S, 672R, 734F, 735A, 736A, 788P, 789L, 790S, 802Y, 806K, 832I, 833G, 834W, 835T
CDD
cd08950 (KR_fFAS_SDR_c_like)

790S

putative NAD(P) binding site
645G, 647G, 648A, 651I, 670T, 671S, 672R, 734F, 735A, 736A, 788P, 789L, 790S, 802Y, 806K, 832I, 833G, 834W, 835T
CDD
cd08950 (KR_fFAS_SDR_c_like)

802Y

putative NAD(P) binding site
645G, 647G, 648A, 651I, 670T, 671S, 672R, 734F, 735A, 736A, 788P, 789L, 790S, 802Y, 806K, 832I, 833G, 834W, 835T
CDD
cd08950 (KR_fFAS_SDR_c_like)

806K

putative NAD(P) binding site
645G, 647G, 648A, 651I, 670T, 671S, 672R, 734F, 735A, 736A, 788P, 789L, 790S, 802Y, 806K, 832I, 833G, 834W, 835T
CDD
cd08950 (KR_fFAS_SDR_c_like)

832I

putative NAD(P) binding site
645G, 647G, 648A, 651I, 670T, 671S, 672R, 734F, 735A, 736A, 788P, 789L, 790S, 802Y, 806K, 832I, 833G, 834W, 835T
CDD
cd08950 (KR_fFAS_SDR_c_like)

833G

putative NAD(P) binding site
645G, 647G, 648A, 651I, 670T, 671S, 672R, 734F, 735A, 736A, 788P, 789L, 790S, 802Y, 806K, 832I, 833G, 834W, 835T
CDD
cd08950 (KR_fFAS_SDR_c_like)

834W

putative NAD(P) binding site
645G, 647G, 648A, 651I, 670T, 671S, 672R, 734F, 735A, 736A, 788P, 789L, 790S, 802Y, 806K, 832I, 833G, 834W, 835T
CDD
cd08950 (KR_fFAS_SDR_c_like)

835T

putative NAD(P) binding site
645G, 647G, 648A, 651I, 670T, 671S, 672R, 734F, 735A, 736A, 788P, 789L, 790S, 802Y, 806K, 832I, 833G, 834W, 835T
CDD
cd08950 (KR_fFAS_SDR_c_like)
active site cd08950

758M

active site
758M, 790S, 802Y, 806K
CDD
cd08950 (KR_fFAS_SDR_c_like)

790S

active site
758M, 790S, 802Y, 806K
CDD
cd08950 (KR_fFAS_SDR_c_like)

802Y

active site
758M, 790S, 802Y, 806K
CDD
cd08950 (KR_fFAS_SDR_c_like)

806K

active site
758M, 790S, 802Y, 806K
CDD
cd08950 (KR_fFAS_SDR_c_like)
active site cd00828

1,267C

active site
1,267C, 1,507H, 1,548H
CDD
cd00828 (elong_cond_enzymes)

1,507H

active site
1,267C, 1,507H, 1,548H
CDD
cd00828 (elong_cond_enzymes)

1,548H

active site
1,267C, 1,507H, 1,548H
CDD
cd00828 (elong_cond_enzymes)

Protein sequence

>MIA_02580_1
MKPEVEQELAHILLTELLAYQFASPVRWIETQDVILKDYNAERVVEIGPSPTLAGMATRTLKAKYESYDAALSLQRQVLC
YSKDTKEIYYTPDPPAEPEAPAAAPAAVAAPAAAPAAAAAPVAAAPAAGPAAAVDDTPVKAIEILHALVAQKLKKTLDQV
PLSKSIKDLVGGKSTVQNEILGDLGKEFGGAPDKPEEVPLGELAEAFQQSFSGQLGKQSSSLISKLMSSKMPGGFSITSA
RKYLSDRYGLGQGRQDSVFLVAITQEPKNRLGAEPEAKAFLDECANKYAASAGISLSAGAGAGAGGAGAAAGGAVIDSAA
FEELTKDQKYLVRQQLELFANYLKVDIRKGDKALLAQKEATALLQTELDLWNQEHGEFYAQGIKPVFSPLKARTYDSYWN
WARQDALKMFWDVIFGKLDTVDREIVANCISLMNRANPTFIDFVQYHMDHVPTERGATYKLVKELGIQLLENCKESLNAD
PVYKDVNYPTGPQTTVDEKGNMSYKEVPRPSVRKLEHYVQEMAQGGPITKDPKANAQAELARIYQTLSSQNSNSASKTKL
DSLYAELNKVIEAINADTSSAKTSEKIDRVSASVPKDTVPFLHLKSRSGDEWSYDRTLSGAYLDGLETAAKEGITFANKN
VLITGAGAGSIGADILQGLLSGGAKVVVTTSRYSRQVTEFYQSIYTRYGAAGSTLIVVPFNQASKTDVLGLVDFIYNDPK
KGGLGWDLDFVIPFAAIPENGIEIDGIDSKSELAHRLMLTNVLRLVGAVKTHKANNGVITRPAQVILPLSPNHGTFGADG
LYSESKLSLETLFNRWHSESWGPYLTICGAVIGWTRGTGLMSQNNAIAEGVEKMGVRTFSQKEMAFNILGLLTPQIVKLA
QQSPVFADLNGGLQFIENLKDLTGRLRSELAEKTDIRRAVSIETAIEHRVTNGETADAPYRRVVVQPRANETYAFPTLKS
FDELKKVAPELEGMLDLERVVVVTGFAEVGPWGNARTRWQMEANGTFSLEGAIEMAWIMGLIKHHNGPLKGKPYSGWVDA
KTNEPVDDKDIKAKYEAYVLDHSGIRLIEPELFNGYDPLKKQFLQEVIIEHDLEPFESSKEAADQFKLEQGDKVETFEIE
GTDQFTVRFLKGARILVPKALKFNRLVAGQIPTGWDARRYGISEDTISQVDPVTLYALVSTVEALMASGITDPFEFYKYV
HLSEVGNCSGSGMGGVSALRGMFKDRYLDIPVQNDILQESFINTMSAWVNMLLISSSGPIKTPVGACATSIESADVGIET
ILSGKAKIVFIGGYDDFQEEGSYEFGNMKATSDSTTEEAHGRTPAEMSRPTSTSRSGFMEAQGAGIQVAMSAKLAIEMGV
PIYGIVALTATATDKIGRSVPAPGRGILTTARENQDNKFPHRLLDIKYRRKQLEKRRTQVKEWLNDEYEYLADEVEALKK
GDDKFDAATYYKERAEQIDFEAARQEKEARNHWGNEFWKQDPRIAPLRGALATFNLTIDDIGIASFHGTSTKANDKNESA
TINDMLNHLGRSKGNPVLGVFQKYLTGHPKGAAGAWMLNGVLQILNSGIVPGNRNADNVDPVMEKFDKILYPSITMQTDG
IKAASVTSFGFGQKGAQLIAIHADYVLAALSKSQYEEYAKKVAARYKKAYRYMHNAIAEQTMFVAKNKPPYTDDQESSVY
LNPLARVDYDKKTESLIFNQKKIVQAKYGSSSGHTEEVVKALAQSNTSSNVGVDVELLSAVNIENETFLERNFTEAERKY
CAAQADPQASFTGTWSAKEAVFKALNTAGKGAGAALDEIEILRSPKGAPYVVLHKDAEAAAKKEGVKSVKISISHNEFQA
VAVAIAEK

GO term prediction

Biological Process

GO:0006633 fatty acid biosynthetic process
GO:0008152 metabolic process
GO:0042759 long-chain fatty acid biosynthetic process

Molecular Function

GO:0000287 magnesium ion binding
GO:0003824 catalytic activity
GO:0004312 fatty acid synthase activity
GO:0008897 holo-[acyl-carrier-protein] synthase activity

Cellular Component

None predicted.

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Entry type

Status

Per-residue features