MIA_02426_1

Protein

Length

1,576 amino acids

Protein function

EGGNOG:	0PFY9	REV3	DNA polymerase
SGD closest match:	S000006088	REV3	DNA polymerase zeta catalytic subunit
CGD closest match:	CAL0000176488	REV3	DNA polymerase

Protein alignments

	%id	Aln length
A0A0J9X563_GEOCN	43.754%	1625	DNA polymerase OS=Geotrichum candidum GN=BN980_GECA02s02595g PE=3 SV=1
UniRef50_A0A0J9X563	43.754%	1625	DNA polymerase n=1 Tax=Geotrichum candidum TaxID=1173061 RepID=A0A0J9X563_GEOCN
MCA_04178_1	57.455%	1006	MCA_04178_1
A0A161HKH0_9ASCO	49.550%	1001	DNA polymerase OS=Sugiyamaella lignohabitans GN=REV3 PE=3 SV=1
A0A060T9Z7_BLAAD	46.742%	1059	DNA polymerase OS=Blastobotrys adeninivorans GN=GNLVRS02_ARAD1D19118g PE=3 SV=1
A0A1E3PNB5_9ASCO	47.239%	1014	DNA polymerase (Fragment) OS=Nadsonia fulvescens var. elongata DSM 6958 GN=NADFUDRAFT_8577 PE=3 SV=1
Q6CB61_YARLI	45.243%	946	DNA polymerase OS=Yarrowia lipolytica (strain CLIB 122 / E 150) GN=YALI0_C21648g PE=3 SV=1
DPOZ_YEAST	42.857%	980	DNA polymerase zeta catalytic subunit OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=REV3 PE=1 SV=1
A0A1D8PKE9_CANAL	44.876%	1005	DNA polymerase OS=Candida albicans (strain SC5314 / ATCC MYA-2876) GN=REV3 PE=3 SV=1
A0A1E4TKE0_9ASCO	41.622%	752	DNA polymerase OS=Tortispora caseinolytica NRRL Y-17796 GN=CANCADRAFT_21996 PE=3 SV=1

Mitochondrial localization by mitoprotII

Probability of mitochondrial location: 0.3276

Protein family membership

DNA-directed DNA polymerase, family B (IPR006172)

Domains and repeats

Domain
52 - 204
Ribonuclease H-like domain (IPR012337)
DNA-directed DNA polymerase, family B, exonuclease domain (IPR006133)
727 - 996
Ribonuclease H-like domain (IPR012337)
DNA-directed DNA polymerase, family B, exonuclease domain (IPR006133)
1,037 - 1,296
DNA polymerase, palm domain (IPR023211)
Domain
983 - 1,437
DNA-directed DNA polymerase, family B, multifunctional domain (IPR006134)
1,475 - 1,552
C4-type zinc-finger of DNA polymerase delta (IPR025687)

1 200 400 600 800 1000 1200 1400 1576

Detailed signature matches

IPR006172

DNA-directed DNA polymerase, family B
1. PR00106 (DNAPOLB)
  
  1,216 - 1,224
  
  PRINTS
  PR00106 (DNAPOLB)
  
  1,159 - 1,171
  
  PRINTS
  PR00106 (DNAPOLB)
  
  1,049 - 1,062
  
  PRINTS
  PR00106 (DNAPOLB)
2. SM00486 (polmehr3)
  
  749 - 1,230
  
  SMART
  SM00486 (polmehr3)
IPR012337

Ribonuclease H-like domain
1. SSF53098 (Ribonucle...)
  
  749 - 996
  
  SUPERFAMILY
  SSF53098 (Ribonuclease H-like)
  
  52 - 197
  
  SUPERFAMILY
  SSF53098 (Ribonuclease H-like)
2. G3DSA:3.30.42...
  
  727 - 973
  
  GENE3D
  G3DSA:3.30.420.10 (G3DSA:3.30.420.10)
IPR006133

DNA-directed DNA polymerase, family B, exonuclease domain
1. PF03104 (DNA_pol_B_...)
  
  760 - 923
  
  Pfam
  PF03104 (DNA_pol_B_exo1)
  
  63 - 204
  
  Pfam
  PF03104 (DNA_pol_B_exo1)
IPR006134

DNA-directed DNA polymerase, family B, multifunctional domain
1. PF00136 (DNA_pol_B)
  
  983 - 1,437
  
  Pfam
  PF00136 (DNA_pol_B)
IPR023211

DNA polymerase, palm domain
1. G3DSA:3.90.16...
  
  1,037 - 1,296
  
  GENE3D
  G3DSA:3.90.1600.10 (G3DSA:3.90.1600.10)
IPR025687

C4-type zinc-finger of DNA polymerase delta
1. PF14260 (zf-C4pol)
  
  1,475 - 1,552
  
  Pfam
  PF14260 (zf-C4pol)
IPR017964

DNA-directed DNA polymerase, family B, conserved site
1. PS00116 (DNA_POLYME...)
  
  1,218 - 1,226
  
  PROSITE patterns
  PS00116 (DNA_POLYMERASE_B)

no IPR

Unintegrated signatures

G3DSA:3.30.34...

44 - 214

GENE3D
G3DSA:3.30.342.10 (G3DSA:3.30.342.10)
SSF56672 (DNA/RNA p...)

997 - 1,452

SUPERFAMILY
SSF56672 (DNA/RNA polymerases)
TMhelix

121 - 143

TMHMM
TMhelix (TMhelix)
cd05534 (POLBc_zeta)

997 - 1,444

CDD
cd05534 (POLBc_zeta)
cd05778 (DNA_polB_z...)

750 - 971

CDD
cd05778 (DNA_polB_zeta_exo)
mobidb-lite (disord...)

455 - 477

MobiDB Lite
mobidb-lite (disorder_prediction)

Residue annotation

active site cd05534

1,053D

active site
1,053D, 1,056S, 1,057L, 1,058Y, 1,135R, 1,163K, 1,167N, 1,222D
CDD
cd05534 (POLBc_zeta)

1,056S

active site
1,053D, 1,056S, 1,057L, 1,058Y, 1,135R, 1,163K, 1,167N, 1,222D
CDD
cd05534 (POLBc_zeta)

1,057L

active site
1,053D, 1,056S, 1,057L, 1,058Y, 1,135R, 1,163K, 1,167N, 1,222D
CDD
cd05534 (POLBc_zeta)

1,058Y

active site
1,053D, 1,056S, 1,057L, 1,058Y, 1,135R, 1,163K, 1,167N, 1,222D
CDD
cd05534 (POLBc_zeta)

1,135R

active site
1,053D, 1,056S, 1,057L, 1,058Y, 1,135R, 1,163K, 1,167N, 1,222D
CDD
cd05534 (POLBc_zeta)

1,163K

active site
1,053D, 1,056S, 1,057L, 1,058Y, 1,135R, 1,163K, 1,167N, 1,222D
CDD
cd05534 (POLBc_zeta)

1,167N

active site
1,053D, 1,056S, 1,057L, 1,058Y, 1,135R, 1,163K, 1,167N, 1,222D
CDD
cd05534 (POLBc_zeta)

1,222D

active site
1,053D, 1,056S, 1,057L, 1,058Y, 1,135R, 1,163K, 1,167N, 1,222D
CDD
cd05534 (POLBc_zeta)
metal-binding site...

1,220D

metal-binding site
1,220D, 1,222D, 1,223S
CDD
cd05534 (POLBc_zeta)

1,222D

metal-binding site
1,220D, 1,222D, 1,223S
CDD
cd05534 (POLBc_zeta)

1,223S

metal-binding site
1,220D, 1,222D, 1,223S
CDD
cd05534 (POLBc_zeta)

Protein sequence

>MIA_02426_1
MDKTGQQLRILYRKPEDCAVEVQINNLDYYQTSPTAFDRQTNPVDWSSRPLPSIPVIRIYGTTAHDQHVMVHIHGIFPYL
YVEYTGSLVEYEVRNYIRKLNKAINDALILAYRRNKDSPNSVFVANIVLCKGVPFYGFHVGWVPYLKIYLLNPSHISKLS
ELLNTGSILGTKMQPYESHIPYILQFLTDFNLTGCGLLRLSAALFRGPLSSENIGLGSQSVLHADGLFHNKSVSPNLVLP
IRYFPKMATCALEIDVDASQILNRENVVERNLHHDFSEAFTSKKNQEKNTSNGDKTNFESRYTGGIKYVNSLKEVWLDEY
QRRHMEGGGTYTSPQDSISRIASSNECERWKLGCELEKEVRARVQQAHKEKNWGKTFQNFTIQRDYIFAVPKALKTVNVM
FTPLELVLTRLEKLNMLPSEPNENKEFENMKEKYDNSDDEKIENLSENLEETAEKKIKGTKDKDDNPEHFKKKHLQNYDS
KIETENVKDGGLTDSAAGFISELDSSDFSFSSFEFSLPEISKTKTSTRKRSAEPDFEESISKPKVLCSSTKNLVATSQYT
IPQTFTFSQEQLSFNTASEKQNFSQILSVFPSSKKTLAFSLKPPTKSEVQASWKLFKLPTETSRKPFYGIPSDIPKHPVV
FSGVEYNVKSNTAPFLPPFKFSSSLNLDEVANILNKTKPKITGKVLKYNVIPPSFSEVQKWLELETQEKANHQLLYSQVN
GPTPKNKYGFKFATQKRNESRKINIATTKYLSSMTLELHISTRKDLHPDPNKDPVLALFWGYQSEYMSEDPVEGCIFVAK
DIHEQKKIASILSKSLIVKFVSLEVDLIDELINTVLDYDPDILSGYEINSASWGYIIQRSRTKYEYDLCEMLSRVLDKAN
VKVGDRWGYTHASAIRISGRHMLNIWRVLRNELNLLKYTLENVAFHVLHYRIPFYTFESLTRWYDSTLVSETEITIKYHI
QRLRVSMDLMDSQEIITRTCEEARIIGIDFYSVFYRGSQYKVESILTRIAKAENFIMISPSKKQVAEQNAIECIPLILEP
KTNFYTSPIVVLDFQSLYPSVMIAYNYCYSTCLGRIHNWRGRNKLGVSDLKLPAGLLSLLQRENITIAPNGMMYVESNIR
KSLLAKMLTEFLDTRVMVKHGMKTNKDNAAFQKLMNSRQLALKLIANVTYGYTSATYSGRMPCVEIADSIVQTGRETMER
AIEHINANQSRWGADVIYGDTDSLFLHFDGRSKEEAFILGNEIANEITNMNPQPMKLKFEKVYWPCILQTKKRYVGYMWE
YPDQKTPVFNAKGIETVRRDGIPAQQKIEEKALHILFDTCDLSKVKEYLLTEFTKIQSRKIPLSDLCFAKEVRLGSYRSR
PPPGAILSKRRAIYDPYSAPQYRERVPYVVVSGPPGARISDRCMAPEDILASDGLFVPDVEYYITNVLIPPLERLLSLAG
ADVRRWYQEMPRKSGFDTALTRVSGIRGFVQNAACRSCGCKVISTNRDYITEMLCTRCRNNLGEVSAKLITRAKKREKKV
KDLDSICQNCAKISPGVLVECISNDCPIYFARAKAASQHNDSLTYDWPLLTKINDW

GO term prediction

Biological Process

None predicted.

Molecular Function

GO:0000166 nucleotide binding
GO:0003676 nucleic acid binding
GO:0003677 DNA binding
GO:0003887 DNA-directed DNA polymerase activity
GO:0008408 3'-5' exonuclease activity

Cellular Component