MIA_02273_1

Protein

Length

451 amino acids

Protein function

EGGNOG:	0PIDE	TUB2	Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain
SGD closest match:	S000001857	TUB2	Tubulin beta chain
CGD closest match:	CAL0000176403	TUB2	Tubulin beta chain

Protein alignments

	%id	Aln length
MCA_06494_1	81.556%	450	MCA_06494_1
TBB2_GEOCN	80.963%	436	Tubulin beta-2 chain OS=Geotrichum candidum PE=3 SV=2
A0A060TBL7_BLAAD	80.896%	424	Tubulin beta chain OS=Blastobotrys adeninivorans GN=GNLVRS02_ARAD1D32890g PE=3 SV=1
A0A1E3PQU8_9ASCO	73.756%	442	Tubulin beta chain OS=Nadsonia fulvescens var. elongata DSM 6958 GN=NADFUDRAFT_20588 PE=3 SV=1
Q6C4G6_YARLI	75.000%	424	Tubulin beta chain OS=Yarrowia lipolytica (strain CLIB 122 / E 150) GN=YALI0_E26961g PE=3 SV=1
A0A1E4TC50_9ASCO	68.822%	433	Tubulin beta chain OS=Tortispora caseinolytica NRRL Y-17796 GN=CANCADRAFT_3974 PE=3 SV=1
UniRef50_A0A063C9B7	63.333%	420	Beta-tubulin n=1990 Tax=Eukaryota TaxID=2759 RepID=A0A063C9B7_9HYPO
TBB_YEAST	63.318%	428	Tubulin beta chain OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=TUB2 PE=1 SV=2
A0A1D8PC97_CANAL	63.658%	421	Tubulin beta chain OS=Candida albicans (strain SC5314 / ATCC MYA-2876) GN=TUB2 PE=3 SV=1
A0A167FK53_9ASCO	76.833%	341	Tubulin beta chain OS=Sugiyamaella lignohabitans GN=TUB2 PE=3 SV=1

Mitochondrial localization by mitoprotII

Probability of mitochondrial location: 0.0500

Protein family membership

Tubulin (IPR000217)
- Beta tubulin (IPR002453)

Domains and repeats

Domain
3 - 256
Tubulin/FtsZ, GTPase domain (IPR003008)
Domain
237 - 421
Tubulin/FtsZ, C-terminal (IPR008280)
Domain
239 - 376
Tubulin/FtsZ, 2-layer sandwich domain (IPR018316)

1 50 100 150 200 250 300 350 400 451

Detailed signature matches

IPR000217

Tubulin
1. PR01161 (TUBULIN)
  
  183 - 203
  
  PRINTS
  PR01161 (TUBULIN)
  
  44 - 63
  
  PRINTS
  PR01161 (TUBULIN)
  
  125 - 143
  
  PRINTS
  PR01161 (TUBULIN)
  
  2 - 22
  
  PRINTS
  PR01161 (TUBULIN)
  
  144 - 165
  
  PRINTS
  PR01161 (TUBULIN)
  
  99 - 123
  
  PRINTS
  PR01161 (TUBULIN)
  
  363 - 391
  
  PRINTS
  PR01161 (TUBULIN)
  
  169 - 182
  
  PRINTS
  PR01161 (TUBULIN)
  
  86 - 97
  
  PRINTS
  PR01161 (TUBULIN)
IPR002453

Beta tubulin
1. PR01163 (BETATUBULIN)
  
  238 - 251
  
  PRINTS
  PR01163 (BETATUBULIN)
  
  258 - 274
  
  PRINTS
  PR01163 (BETATUBULIN)
  
  81 - 92
  
  PRINTS
  PR01163 (BETATUBULIN)
  
  145 - 157
  
  PRINTS
  PR01163 (BETATUBULIN)
  
  205 - 217
  
  PRINTS
  PR01163 (BETATUBULIN)
  
  101 - 119
  
  PRINTS
  PR01163 (BETATUBULIN)
  
  370 - 381
  
  PRINTS
  PR01163 (BETATUBULIN)
  
  405 - 423
  
  PRINTS
  PR01163 (BETATUBULIN)
  
  322 - 336
  
  PRINTS
  PR01163 (BETATUBULIN)
  
  223 - 232
  
  PRINTS
  PR01163 (BETATUBULIN)
  
  340 - 363
  
  PRINTS
  PR01163 (BETATUBULIN)
  
  34 - 51
  
  PRINTS
  PR01163 (BETATUBULIN)
  
  304 - 322
  
  PRINTS
  PR01163 (BETATUBULIN)
IPR003008

Tubulin/FtsZ, GTPase domain
1. PF00091 (Tubulin)
  
  6 - 204
  
  Pfam
  PF00091 (Tubulin)
2. SM00864 (Tubulin_4)
  
  40 - 237
  
  SMART
  SM00864 (Tubulin_4)
3. G3DSA:3.40.50...
  
  3 - 256
  
  GENE3D
  G3DSA:3.40.50.1440 (G3DSA:3.40.50.1440)
4. SSF52490 (Tubulin n...)
  
  5 - 236
  
  SUPERFAMILY
  SSF52490 (Tubulin nucleotide-binding domain-like)
IPR008280

Tubulin/FtsZ, C-terminal
1. SSF55307 (Tubulin C...)
  
  237 - 421
  
  SUPERFAMILY
  SSF55307 (Tubulin C-terminal domain-like)
IPR018316

Tubulin/FtsZ, 2-layer sandwich domain
1. PF03953 (Tubulin_C)
  
  254 - 374
  
  Pfam
  PF03953 (Tubulin_C)
2. G3DSA:3.30.13...
  
  257 - 366
  
  GENE3D
  G3DSA:3.30.1330.20 (G3DSA:3.30.1330.20)
3. SM00865 (Tubulin_C_4)
  
  239 - 376
  
  SMART
  SM00865 (Tubulin_C_4)
IPR017975

Tubulin, conserved site
1. PS00227 (TUBULIN)
  
  133 - 139
  
  PROSITE patterns
  PS00227 (TUBULIN)

no IPR

Unintegrated signatures

G3DSA:1.10.28...

367 - 433

GENE3D
G3DSA:1.10.287.600 (G3DSA:1.10.287.600)
cd02187 (beta_tubulin)

6 - 419

CDD
cd02187 (beta_tubulin)

Residue annotation

nucleotide binding...

7Q

nucleotide binding site
7Q, 131S, 133G, 134G, 135G, 136T, 137G, 174E, 197N, 215H, 219N
CDD
cd02187 (beta_tubulin)

131S

nucleotide binding site
7Q, 131S, 133G, 134G, 135G, 136T, 137G, 174E, 197N, 215H, 219N
CDD
cd02187 (beta_tubulin)

133G

nucleotide binding site
7Q, 131S, 133G, 134G, 135G, 136T, 137G, 174E, 197N, 215H, 219N
CDD
cd02187 (beta_tubulin)

134G

nucleotide binding site
7Q, 131S, 133G, 134G, 135G, 136T, 137G, 174E, 197N, 215H, 219N
CDD
cd02187 (beta_tubulin)

135G

nucleotide binding site
7Q, 131S, 133G, 134G, 135G, 136T, 137G, 174E, 197N, 215H, 219N
CDD
cd02187 (beta_tubulin)

136T

nucleotide binding site
7Q, 131S, 133G, 134G, 135G, 136T, 137G, 174E, 197N, 215H, 219N
CDD
cd02187 (beta_tubulin)

137G

nucleotide binding site
7Q, 131S, 133G, 134G, 135G, 136T, 137G, 174E, 197N, 215H, 219N
CDD
cd02187 (beta_tubulin)

174E

nucleotide binding site
7Q, 131S, 133G, 134G, 135G, 136T, 137G, 174E, 197N, 215H, 219N
CDD
cd02187 (beta_tubulin)

197N

nucleotide binding site
7Q, 131S, 133G, 134G, 135G, 136T, 137G, 174E, 197N, 215H, 219N
CDD
cd02187 (beta_tubulin)

215H

nucleotide binding site
7Q, 131S, 133G, 134G, 135G, 136T, 137G, 174E, 197N, 215H, 219N
CDD
cd02187 (beta_tubulin)

219N

nucleotide binding site
7Q, 131S, 133G, 134G, 135G, 136T, 137G, 174E, 197N, 215H, 219N
CDD
cd02187 (beta_tubulin)
Taxol binding site...

14N

Taxol binding site
14N, 15T, 18E, 19E, 208L, 217D, 220A, 221L, 227S, 263F, 265P, 266L, 267V, 351P, 352K, 353G
CDD
cd02187 (beta_tubulin)

15T

Taxol binding site
14N, 15T, 18E, 19E, 208L, 217D, 220A, 221L, 227S, 263F, 265P, 266L, 267V, 351P, 352K, 353G
CDD
cd02187 (beta_tubulin)

18E

Taxol binding site
14N, 15T, 18E, 19E, 208L, 217D, 220A, 221L, 227S, 263F, 265P, 266L, 267V, 351P, 352K, 353G
CDD
cd02187 (beta_tubulin)

19E

Taxol binding site
14N, 15T, 18E, 19E, 208L, 217D, 220A, 221L, 227S, 263F, 265P, 266L, 267V, 351P, 352K, 353G
CDD
cd02187 (beta_tubulin)

208L

Taxol binding site
14N, 15T, 18E, 19E, 208L, 217D, 220A, 221L, 227S, 263F, 265P, 266L, 267V, 351P, 352K, 353G
CDD
cd02187 (beta_tubulin)

217D

Taxol binding site
14N, 15T, 18E, 19E, 208L, 217D, 220A, 221L, 227S, 263F, 265P, 266L, 267V, 351P, 352K, 353G
CDD
cd02187 (beta_tubulin)

220A

Taxol binding site
14N, 15T, 18E, 19E, 208L, 217D, 220A, 221L, 227S, 263F, 265P, 266L, 267V, 351P, 352K, 353G
CDD
cd02187 (beta_tubulin)

221L

Taxol binding site
14N, 15T, 18E, 19E, 208L, 217D, 220A, 221L, 227S, 263F, 265P, 266L, 267V, 351P, 352K, 353G
CDD
cd02187 (beta_tubulin)

227S

Taxol binding site
14N, 15T, 18E, 19E, 208L, 217D, 220A, 221L, 227S, 263F, 265P, 266L, 267V, 351P, 352K, 353G
CDD
cd02187 (beta_tubulin)

263F

Taxol binding site
14N, 15T, 18E, 19E, 208L, 217D, 220A, 221L, 227S, 263F, 265P, 266L, 267V, 351P, 352K, 353G
CDD
cd02187 (beta_tubulin)

265P

Taxol binding site
14N, 15T, 18E, 19E, 208L, 217D, 220A, 221L, 227S, 263F, 265P, 266L, 267V, 351P, 352K, 353G
CDD
cd02187 (beta_tubulin)

266L

Taxol binding site
14N, 15T, 18E, 19E, 208L, 217D, 220A, 221L, 227S, 263F, 265P, 266L, 267V, 351P, 352K, 353G
CDD
cd02187 (beta_tubulin)

267V

Taxol binding site
14N, 15T, 18E, 19E, 208L, 217D, 220A, 221L, 227S, 263F, 265P, 266L, 267V, 351P, 352K, 353G
CDD
cd02187 (beta_tubulin)

351P

Taxol binding site
14N, 15T, 18E, 19E, 208L, 217D, 220A, 221L, 227S, 263F, 265P, 266L, 267V, 351P, 352K, 353G
CDD
cd02187 (beta_tubulin)

352K

Taxol binding site
14N, 15T, 18E, 19E, 208L, 217D, 220A, 221L, 227S, 263F, 265P, 266L, 267V, 351P, 352K, 353G
CDD
cd02187 (beta_tubulin)

353G

Taxol binding site
14N, 15T, 18E, 19E, 208L, 217D, 220A, 221L, 227S, 263F, 265P, 266L, 267V, 351P, 352K, 353G
CDD
cd02187 (beta_tubulin)
alpha/beta domain ...

63P

alpha/beta domain interface
63P, 64A, 91G, 92N, 93N, 96K, 172V, 174E, 215H
CDD
cd02187 (beta_tubulin)

64A

alpha/beta domain interface
63P, 64A, 91G, 92N, 93N, 96K, 172V, 174E, 215H
CDD
cd02187 (beta_tubulin)

91G

alpha/beta domain interface
63P, 64A, 91G, 92N, 93N, 96K, 172V, 174E, 215H
CDD
cd02187 (beta_tubulin)

92N

alpha/beta domain interface
63P, 64A, 91G, 92N, 93N, 96K, 172V, 174E, 215H
CDD
cd02187 (beta_tubulin)

93N

alpha/beta domain interface
63P, 64A, 91G, 92N, 93N, 96K, 172V, 174E, 215H
CDD
cd02187 (beta_tubulin)

96K

alpha/beta domain interface
63P, 64A, 91G, 92N, 93N, 96K, 172V, 174E, 215H
CDD
cd02187 (beta_tubulin)

172V

alpha/beta domain interface
63P, 64A, 91G, 92N, 93N, 96K, 172V, 174E, 215H
CDD
cd02187 (beta_tubulin)

174E

alpha/beta domain interface
63P, 64A, 91G, 92N, 93N, 96K, 172V, 174E, 215H
CDD
cd02187 (beta_tubulin)

215H

alpha/beta domain interface
63P, 64A, 91G, 92N, 93N, 96K, 172V, 174E, 215H
CDD
cd02187 (beta_tubulin)
beta/alpha domain ...

124Q

beta/alpha domain interface
124Q, 238Q, 239L, 240N, 244R, 249N, 251V, 252P, 253Y, 315A, 316M, 317K, 320E, 340N, 341N, 342V, 343Q, 344T
CDD
cd02187 (beta_tubulin)

238Q

beta/alpha domain interface
124Q, 238Q, 239L, 240N, 244R, 249N, 251V, 252P, 253Y, 315A, 316M, 317K, 320E, 340N, 341N, 342V, 343Q, 344T
CDD
cd02187 (beta_tubulin)

239L

beta/alpha domain interface
124Q, 238Q, 239L, 240N, 244R, 249N, 251V, 252P, 253Y, 315A, 316M, 317K, 320E, 340N, 341N, 342V, 343Q, 344T
CDD
cd02187 (beta_tubulin)

240N

beta/alpha domain interface
124Q, 238Q, 239L, 240N, 244R, 249N, 251V, 252P, 253Y, 315A, 316M, 317K, 320E, 340N, 341N, 342V, 343Q, 344T
CDD
cd02187 (beta_tubulin)

244R

beta/alpha domain interface
124Q, 238Q, 239L, 240N, 244R, 249N, 251V, 252P, 253Y, 315A, 316M, 317K, 320E, 340N, 341N, 342V, 343Q, 344T
CDD
cd02187 (beta_tubulin)

249N

beta/alpha domain interface
124Q, 238Q, 239L, 240N, 244R, 249N, 251V, 252P, 253Y, 315A, 316M, 317K, 320E, 340N, 341N, 342V, 343Q, 344T
CDD
cd02187 (beta_tubulin)

251V

beta/alpha domain interface
124Q, 238Q, 239L, 240N, 244R, 249N, 251V, 252P, 253Y, 315A, 316M, 317K, 320E, 340N, 341N, 342V, 343Q, 344T
CDD
cd02187 (beta_tubulin)

252P

beta/alpha domain interface
124Q, 238Q, 239L, 240N, 244R, 249N, 251V, 252P, 253Y, 315A, 316M, 317K, 320E, 340N, 341N, 342V, 343Q, 344T
CDD
cd02187 (beta_tubulin)

253Y

beta/alpha domain interface
124Q, 238Q, 239L, 240N, 244R, 249N, 251V, 252P, 253Y, 315A, 316M, 317K, 320E, 340N, 341N, 342V, 343Q, 344T
CDD
cd02187 (beta_tubulin)

315A

beta/alpha domain interface
124Q, 238Q, 239L, 240N, 244R, 249N, 251V, 252P, 253Y, 315A, 316M, 317K, 320E, 340N, 341N, 342V, 343Q, 344T
CDD
cd02187 (beta_tubulin)

316M

beta/alpha domain interface
124Q, 238Q, 239L, 240N, 244R, 249N, 251V, 252P, 253Y, 315A, 316M, 317K, 320E, 340N, 341N, 342V, 343Q, 344T
CDD
cd02187 (beta_tubulin)

317K

beta/alpha domain interface
124Q, 238Q, 239L, 240N, 244R, 249N, 251V, 252P, 253Y, 315A, 316M, 317K, 320E, 340N, 341N, 342V, 343Q, 344T
CDD
cd02187 (beta_tubulin)

320E

beta/alpha domain interface
124Q, 238Q, 239L, 240N, 244R, 249N, 251V, 252P, 253Y, 315A, 316M, 317K, 320E, 340N, 341N, 342V, 343Q, 344T
CDD
cd02187 (beta_tubulin)

340N

beta/alpha domain interface
124Q, 238Q, 239L, 240N, 244R, 249N, 251V, 252P, 253Y, 315A, 316M, 317K, 320E, 340N, 341N, 342V, 343Q, 344T
CDD
cd02187 (beta_tubulin)

341N

beta/alpha domain interface
124Q, 238Q, 239L, 240N, 244R, 249N, 251V, 252P, 253Y, 315A, 316M, 317K, 320E, 340N, 341N, 342V, 343Q, 344T
CDD
cd02187 (beta_tubulin)

342V

beta/alpha domain interface
124Q, 238Q, 239L, 240N, 244R, 249N, 251V, 252P, 253Y, 315A, 316M, 317K, 320E, 340N, 341N, 342V, 343Q, 344T
CDD
cd02187 (beta_tubulin)

343Q

beta/alpha domain interface
124Q, 238Q, 239L, 240N, 244R, 249N, 251V, 252P, 253Y, 315A, 316M, 317K, 320E, 340N, 341N, 342V, 343Q, 344T
CDD
cd02187 (beta_tubulin)

344T

beta/alpha domain interface
124Q, 238Q, 239L, 240N, 244R, 249N, 251V, 252P, 253Y, 315A, 316M, 317K, 320E, 340N, 341N, 342V, 343Q, 344T
CDD
cd02187 (beta_tubulin)

Protein sequence

>MIA_02273_1
MRPGSNQVSTAFWNTIIEEHGLDADGHLKPNQDEYSSKRIDVFFSEASNRKYVPRAVAVDLEPATLDTIRSGPLGHIYRP
DNLISGDSGAGNNWAKGFYTEGAELMESVMDAVRHEAEQADSLQGFQLAHSLGGGTGSGLGTLLLTRIKEEYPDRMLSTY
SVLPSPKVSETVTEPYNAVLSFNQLIDHADATYCLDNEALYNICENTLKIARPTHKDLNALIALVMSGVTTCLRYPGQLN
GDLRKLAVNLVPYPRLHFFTAGFAPLVATNAKSFQNYTVPELTQQLFSPTNVMAACNPYHGKYLTISTIFRGKVAMKEVE
DAIAAARQRNFQYFVEWIPNNVQTSVCNVPPKGLETSATFIANSTAIQELLSRISDQFSRMFKRKAFLHWYTNEGMELAE
FGEASSNLKDLILEYQQYQEAGIDDEIVDEEGLIYEDHAVYDSVVDEYQEA

GO term prediction

Biological Process

GO:0007017 microtubule-based process

Molecular Function

GO:0003924 GTPase activity
GO:0005200 structural constituent of cytoskeleton
GO:0005525 GTP binding

Cellular Component

GO:0005874 microtubule

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Entry type

Status

Per-residue features