Protein

MIA_02109_1

Length
658 amino acids

Browser: contig02:2649422-2651457+

Protein function

EGGNOG:0PJNWGUF1Promotes mitochondrial protein synthesis. May act as a fidelity factor of the translation reaction, by catalyzing a one- codon backward translocation of tRNAs on improperly translocated ribosomes. Binds to mitochondrial ribosomes in a GTP-dependent manner (By similarity)
SGD closest match:S000004280GUF1Translation factor GUF1, mitochondrial
CGD closest match:CAL0000194371GUF1Translation factor GUF1, mitochondrial

Protein alignments

%idAln lengthE-value
MCA_02376_183.654%6240.0MCA_02376_1
A0A0J9X6B8_GEOCN77.338%6310.0Translation factor GUF1, mitochondrial OS=Geotrichum candidum GN=GUF1 PE=3 SV=1
A0A167DK46_9ASCO76.274%6280.0Translation factor GUF1, mitochondrial OS=Sugiyamaella lignohabitans GN=GUF1 PE=3 SV=1
A0A1E3PIL8_9ASCO73.096%6170.0Translation factor GUF1, mitochondrial OS=Nadsonia fulvescens var. elongata DSM 6958 GN=GUF1 PE=3 SV=1
A0A060T6X5_BLAAD68.092%6550.0Translation factor GUF1, mitochondrial OS=Blastobotrys adeninivorans GN=GUF1 PE=3 SV=1
UniRef50_A0A1V2L27270.376%6110.0Translation factor GUF1, mitochondrial n=1 Tax=Cyberlindnera fabianii TaxID=36022 RepID=A0A1V2L272_CYBFA
GUF1_YEAST65.811%6230.0Translation factor GUF1, mitochondrial OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=GUF1 PE=1 SV=2
GUF1_CANAL67.921%6110.0Translation factor GUF1, mitochondrial OS=Candida albicans (strain SC5314 / ATCC MYA-2876) GN=GUF1 PE=3 SV=1
A0A1E4TER5_9ASCO64.532%6090.0Translation factor GUF1, mitochondrial OS=Tortispora caseinolytica NRRL Y-17796 GN=GUF1 PE=3 SV=1
GUF1_YARLI63.754%6180.0Translation factor GUF1, mitochondrial OS=Yarrowia lipolytica (strain CLIB 122 / E 150) GN=GUF1 PE=3 SV=1

Mitochondrial localization by mitoprotII

Probability of mitochondrial location: 0.9932
Predicted cleavage: 26

Protein family membership

Domains and repeats

  1. Domain
  2. Domain
1 100 200 300 400 500 600 658

Detailed signature matches

  1. Family IPR006297
    Elongation factor 4
    1. TIGR01393
    2. MF_00071 (LepA)
  2. Domain IPR027417
    P-loop containing nucleoside triphosphate hydrolase
    1. SSF52540 (P-loop co...)
  3. Domain IPR005225
    Small GTP-binding protein domain
    1. TIGR00231
  4. Domain IPR000795
    Transcription factor, GTP-binding domain
    1. PS51722 (G_TR_2)
    2. PF00009 (GTP_EFTU)
    3. PR00315 (ELONGATNFCT)
  5. Domain IPR009000
    Translation protein, beta-barrel domain
    1. SSF50447 (Translati...)
  6. Domain IPR035647
    EF-G domain III/V-like
    1. SSF54980 (EF-G C-te...)
  7. Domain IPR000640
    Elongation factor EFG, domain V-like
    1. PF00679 (EFG_C)
  8. Domain IPR035654
    Elongation factor 4, domain IV
    1. cd03709 (lepA_C)
  9. Domain IPR013842
    GTP-binding protein LepA, C-terminal
    1. PF06421 (LepA_C)
  10. SITE IPR031157
    Tr-type G domain, conserved site
    1. PS00301 (G_TR_1)
Unintegrated signatures no IPR
Unintegrated signatures
  1. G3DSA:2.40.30.10
  2. G3DSA:3.30.70...
  3. G3DSA:3.30.70...
  4. G3DSA:3.40.50...
  5. cd01890 (LepA)
  6. cd16260 (EF4_III)

Residue annotation

  1. G1 box cd01890
  2. putative GEF inter...
  3. GTP/Mg2+ binding s...
  4. Switch I region cd...
  5. G2 box cd01890
  6. G3 box cd01890
  7. Switch II region c...
  8. G4 box cd01890
  9. G5 box cd01890
  10. S12 interface cd16...

Protein sequence

>MIA_02109_1
MTTVAASRAFRSFVRAPWLGFLSRNNSTVTSKLKPKPGAKTETRVASKELQDRIAAIPIENYRNFSIVAHVDHGKSTLSD
RLLELTGVITPGENKQVLDKLDVERERGITVKAQTCTMLYKYKGQDYLLHLVDTPGHVDFRAEVSRSYASCGGALLVVDA
SQGIQAQTVANFYLAYSLGLKLVPVINKIDLDHANIPRAKEQIEETFELDADEAVCISAKTGQNVESVLPAVIENIPPPK
VRIDSPFRCLLVDSWFDSYVGVVLLVYVVDGNVKKGQKIISAHTQLKYEVKDVGIMYPDRLTTPAGLQAGQVGYIVPGMK
DSTDAHIGDTFMHVDHLVEPLPGFEEQKPMVFVGAFPADGVDFKSLDESLRQLCLNDRSVTLQKETSSALGQGWRLGFLG
TLHASVFRERLEKEYGANIIITAPTVPFKVVWKDGTETIVSNPDHFPESGSLSQKVASLQEPYVEALISVPEEYVGTVIK
LCEDNRGNQVNIQYLSNRQALLKYLLPMSQLVDDFFGKLKGLTKGYGSLDYEDAGYLPSSIVKMELVVNGRSVDALAQVL
HRSQVETKGRDWVKRFKEYLSKSQLFEIVIQAKAGNKIVARETIKAKRKDVLAKLHASDISRRQKLLKKQKEGKKVMRSI
GNVQIPQEAYQSFLAKPS

GO term prediction

Biological Process

None predicted.

Molecular Function

GO:0003924 GTPase activity
GO:0005525 GTP binding

Cellular Component

None predicted.