Protein

MCA_02376_1

Length
670 amino acids

Gene name: GUF1

Description: Translation factor GUF1, mitochondrial

Browser: contigB:1098319-1100332-

RNA-seq: read pairs 656, FPKM 12.1, percentile rank 29.3% (100% = highest expression)

Protein function

Annotation:GUF1Translation factor GUF1, mitochondrial
KEGG:K21594GUF1 translation factor GUF1, mitochondrial [EC:3.6.5.-]
EGGNOG:0PJNWGUF1Promotes mitochondrial protein synthesis. May act as a fidelity factor of the translation reaction, by catalyzing a one- codon backward translocation of tRNAs on improperly translocated ribosomes. Binds to mitochondrial ribosomes in a GTP-dependent manner (By similarity)
SGD closest match:S000004280GUF1Translation factor GUF1, mitochondrial
CGD closest match:CAL0000194371GUF1Translation factor GUF1, mitochondrial

Protein alignments

%idAln lengthE-value
MIA_02109_181.13%6520.0MIA_02109_1
A0A0J9X6B8_GEOCN75.19%6570.0Translation factor GUF1, mitochondrial OS=Geotrichum candidum GN=GUF1 PE=3 SV=1
A0A167DK46_9ASCO73.24%6390.0Translation factor GUF1, mitochondrial OS=Sugiyamaella lignohabitans GN=GUF1 PE=3 SV=1
A0A060T6X5_BLAAD68.99%6450.0Translation factor GUF1, mitochondrial OS=Blastobotrys adeninivorans GN=GUF1 PE=3 SV=1
A0A1E3PIL8_9ASCO70.45%6160.0Translation factor GUF1, mitochondrial OS=Nadsonia fulvescens var. elongata DSM 6958 GN=GUF1 PE=3 SV=1
UniRef50_A0A1V2L27269.77%6120.0Translation factor GUF1, mitochondrial n=1 Tax=Cyberlindnera fabianii TaxID=36022 RepID=A0A1V2L272_CYBFA
GUF1_CANAL65.83%6410.0Translation factor GUF1, mitochondrial OS=Candida albicans (strain SC5314 / ATCC MYA-2876) GN=GUF1 PE=3 SV=1
GUF1_YEAST65.70%6210.0Translation factor GUF1, mitochondrial OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=GUF1 PE=1 SV=2
A0A1E4TER5_9ASCO64.80%6080.0Translation factor GUF1, mitochondrial OS=Tortispora caseinolytica NRRL Y-17796 GN=GUF1 PE=3 SV=1
GUF1_YARLI63.27%6180.0Translation factor GUF1, mitochondrial OS=Yarrowia lipolytica (strain CLIB 122 / E 150) GN=GUF1 PE=3 SV=1

Mitochondrial localization by mitoprotII

Probability of mitochondrial location: 0.9987
Predicted cleavage: 43

Protein family membership

Domains and repeats

  1. Domain
  2. Domain
1 100 200 300 400 500 600 670

Detailed signature matches

  1. Family IPR006297
    Elongation factor 4
    1. TIGR01393
    2. MF_00071 (LepA)
  2. Domain IPR027417
    P-loop containing nucleoside triphosphate hydrolase
    1. SSF52540 (P-loop co...)
  3. Domain IPR005225
    Small GTP-binding protein domain
    1. TIGR00231
  4. Domain IPR000795
    Transcription factor, GTP-binding domain
    1. PS51722 (G_TR_2)
    2. PF00009 (GTP_EFTU)
    3. PR00315 (ELONGATNFCT)
  5. Domain IPR009000
    Translation protein, beta-barrel domain
    1. SSF50447 (Translati...)
  6. Domain IPR035647
    EF-G domain III/V-like
    1. SSF54980 (EF-G C-te...)
  7. Domain IPR000640
    Elongation factor EFG, domain V-like
    1. PF00679 (EFG_C)
  8. Domain IPR035654
    Elongation factor 4, domain IV
    1. cd03709 (lepA_C)
  9. Domain IPR013842
    GTP-binding protein LepA, C-terminal
    1. PF06421 (LepA_C)
  10. SITE IPR031157
    Tr-type G domain, conserved site
    1. PS00301 (G_TR_1)
Unintegrated signatures no IPR
Unintegrated signatures
  1. G3DSA:2.40.30.10
  2. G3DSA:3.30.70...
  3. G3DSA:3.30.70...
  4. G3DSA:3.40.50...
  5. cd01890 (LepA)
  6. cd03699 (EF4_II)
  7. cd16260 (EF4_III)

Residue annotation

  1. G1 box cd01890
  2. putative GEF inter...
  3. GTP/Mg2+ binding s...
  4. Switch I region cd...
  5. G2 box cd01890
  6. G3 box cd01890
  7. Switch II region c...
  8. G4 box cd01890
  9. G5 box cd01890
  10. tRNA binding site ...
  11. S12 interface cd16...

Protein sequence

>MCA_02376_1
MRLLNPTTSPFSFLRPVVLRSFRNIPSVSLSSRKISYTSIRYDVAPKIKAGTRSETKVASKELQDRIMAIPIENYRNFSI
VAHVDHGKSTLSDRLLEYTGVIAPGDANKQVLDKLDVERERGITIKAQTCTMLYSENGQDYLLHLVDTPGHVDFRAEVSR
SYASCGGALLVVDASQGVQAQTVANFYLAYSLGLKLIPVINKIDLGHADIPKAKEQIEETFELDPDEAICISAKMGTNIE
QVLPAVIKSIPPPDVHPDNPFRCLLVDSWFDTYLGVVLLVYIVDGSIKKGAKIISAHTQNKYEVKDVGIMYPDRLSTNIL
KAGQVGYIVPGMKSSLDAFVGDTFMKVDQIVEALPGFEEPKPMVFVGAFPADGEDFKTLDESLRQLLLNDRSVTIQKETS
SALGQGWRLGFLGTLHASVFRERLQKEHGASIIITAPTVPFKIVWKNGEETIISNPDNFPNADMMGKVESLQEPYVEALI
SVPQDYIGTVIRLCEDNRGEQVGIQYLANGQALIKYLIPLSQLVDDFFGKLKGSTKGYGSLDYEDAGYKTSQIVKMELLV
NGRSVDALSQVLHRSQVELKGRDWVKRFKEFVKNQLFEVVIQARAANKIVARETVKAKRKDVLAKLHASDITRRQKLLKK
QKEGKKFMRSIGNVQIPQEAYQSFLSKSQV

GO term prediction

Biological Process

None predicted.

Molecular Function

GO:0003924 GTPase activity
GO:0005525 GTP binding

Cellular Component

None predicted.